Remote-sensing-based analysis of the 1996 surge of Northern Inylchek Glacier, central Tien Shan, Kyrgyzstan

The evolution of Northern Inylchek Glacier and its proglacial lake - Upper Lake Merzbacher - during its 1996 surge and the surrounding decades is analyzed with remote sensing imagery. Overall retreat of the glacier from 1943 to 1996 enlarged the lake to 4 km long and ≈ 100 m deep. The surge in 1996 initiated between 12 September and 7 October and advanced the glacier by 3.7 km to override most of Upper Lake Merzbacher. The surge phase probably ended in December 1996 and involved mean flow velocities across the lower trunk of the glacier that reached 50 m d− 1 over a 32-day period. Water displaced by the surge from Upper Lake Merzbacher, totalling 1.5 × 108 m3 in volume, accelerated filling of Lower Lake Merzbacher downvalley and helped trigger this marginal ice-dammed lake to outburst in a jökulhlaup around late November/early December. The characteristics and duration of the surge render it as similar to temperate glacier surges elsewhere. It may have been facilitated by low basal friction caused by water-saturated sediments in the upper lake bed. Furthermore, bathymetric measurements show that the surge evacuated much sediment into the upper lake, causing its depth to reduce from 20 to 30 m in 1996 to 8 m by 2005 and 2 m by 2011; the corresponding deposition rates imply glacier-catchment specific mean sediment yields of 1.4 to 3.4 × 103 Mg km− 2 a− 1 in the years after the surge. Our study documents novel interactions within a cascade system of glaciers and lakes that exhibits surging and outburst-flood behavior

Multicenter Evaluation of the QIAstat-Dx Respiratory Panel for the Detection of Viruses and Bacteria in Nasopharyngeal Swab Specimens

The QIAstat-Dx Respiratory Panel (QIAstat-Dx RP) is a multiplex in vitro diagnostic test for the qualitative detection of 20 pathogens directly from nasopharyngeal swab (NPS) specimens. The assay is performed using a simple sample-to-answer platform with results available in approximately 69 min. The pathogens identified are adenovirus, coronavirus 229E, coronavirus HKU1, coronavirus NL63, coronavirus OC43, human metapneumovirus A and B, influenza A, influenza A H1, influenza A H3, influenza A H1N1/2009, influenza B, parainfluenza virus 1, parainfluenza virus 2, parainfluenza virus 3, parainfluenza virus 4, rhinovirus/enterovirus, respiratory syncytial virus A and B, Bordetella pertussis, Chlamydophila pneumoniae, and Mycoplasma pneumoniae. This multicenter evaluation provides data obtained from 1,994 prospectively collected and 310 retrospectively collected (archived) NPS specimens with performance compared to that of the BioFire FilmArray Respiratory Panel, version 1.7. The overall percent agreement between QIAstat-Dx RP and the comparator testing was 99.5%. In the prospective cohort, the QIAstat-Dx RP demonstrated a positive percent agreement of 94.0% or greater for the detection of all but four analytes: coronaviruses 229E, NL63, and OC43 and rhinovirus/enterovirus. The test also demonstrated a negative percent agreement of ≥97.9% for all analytes. The QIAstat-Dx RP is a robust and accurate assay for rapid, comprehensive testing for respiratory pathogens

Previous attentional set can induce an attentional blink with task-irrelevant initial targets

Identification of a second target is often impaired by the requirement to process a prior target in a rapid serial visual presentation (RSVP). This is termed the attentional blink. Even when the first target is task-irrelevant an attentional blink may occur providing this first target shares similar features with the second target (contingent capture). An RSVP experiment was undertaken to assess whether this first target can still cause an attentional blink when it did not require a response and did not share any features with the following target. The results revealed that such task-irrelevant targets can induce an attentional blink providing that they were task-relevant on a previous block of trials. This suggests that irrelevant focal stimuli can distract attention on the basis of a previous attentional set

Recurrent \u3cem\u3eStreptococcus equi\u3c/em\u3e subsp. \u3cem\u3ezooepidemicus\u3c/em\u3e Bacteremia in an Infant

We describe a case of an infant with recurrent bacteremia caused by Streptococcus equi subsp. zooepidemicus, likely transmitted from mother to infant. Our case highlights the importance of an epidemiological history and molecular diagnostics in ascertaining insights into transmission, pathogenesis, and optimal management

Asynchronous BCI control using high-frequency SSVEP

<p>Abstract</p> <p>Background</p> <p>Steady-State Visual Evoked Potential (SSVEP) is a visual cortical response evoked by repetitive stimuli with a light source flickering at frequencies above 4 Hz and could be classified into three ranges: low (up to 12 Hz), medium (12-30) and high frequency (> 30 Hz). SSVEP-based Brain-Computer Interfaces (BCI) are principally focused on the low and medium range of frequencies whereas there are only a few projects in the high-frequency range. However, they only evaluate the performance of different methods to extract SSVEP.</p> <p>Methods</p> <p>This research proposed a high-frequency SSVEP-based asynchronous BCI in order to control the navigation of a mobile object on the screen through a scenario and to reach its final destination. This could help impaired people to navigate a robotic wheelchair. There were three different scenarios with different difficulty levels (easy, medium and difficult). The signal processing method is based on Fourier transform and three EEG measurement channels.</p> <p>Results</p> <p>The research obtained accuracies ranging in classification from 65% to 100% with Information Transfer Rate varying from 9.4 to 45 bits/min.</p> <p>Conclusions</p> <p>Our proposed method allows all subjects participating in the study to control the mobile object and to reach a final target without prior training.</p

Bcl-XL Inhibits Membrane Permeabilization by Competing with Bax

Although Bcl-XL and Bax are structurally similar, activated Bax forms large oligomers that permeabilize the outer mitochondrial membrane, thereby committing cells to apoptosis, whereas Bcl-XL inhibits this process. Two different models of Bcl-XL function have been proposed. In one, Bcl-XL binds to an activator, thereby preventing Bax activation. In the other, Bcl-XL binds directly to activated Bax. It has been difficult to sort out which interaction is important in cells, as all three proteins are present simultaneously. We examined the mechanism of Bax activation by tBid and its inhibition by Bcl-XL using full-length recombinant proteins and measuring permeabilization of liposomes and mitochondria in vitro. Our results demonstrate that Bcl-XL and Bax are functionally similar. Neither protein bound to membranes alone. However, the addition of tBid recruited molar excesses of either protein to membranes, indicating that tBid activates both pro- and antiapoptotic members of the Bcl-2 family. Bcl-XL competes with Bax for the activation of soluble, monomeric Bax through interaction with membranes, tBid, or t-Bid-activated Bax, thereby inhibiting Bax binding to membranes, oligomerization, and membrane permeabilization. Experiments in which individual interactions were abolished by mutagenesis indicate that both Bcl-XL–tBid and Bcl-XL–Bax binding contribute to the antiapoptotic function of Bcl-XL. By out-competing Bax for the interactions leading to membrane permeabilization, Bcl-XL ties up both tBid and Bax in nonproductive interactions and inhibits Bax binding to membranes. We propose that because Bcl-XL does not oligomerize it functions like a dominant-negative Bax in the membrane permeabilization process

Multicenter Clinical Evaluation of the Automated Aries Bordetella Assay

Molecular methods offer superior sensitivity and specificity and reduce testing turnaround time from days to hours for detection of Bordetella pertussis and Bordetella parapertussis In this study, we evaluated the performance of the automated PCR-based Aries Bordetella Assay, which detects both B. pertussis and B. parapertussis directly from nasopharyngeal swab specimens. The limits of detection (LoDs) were 1,800 CFU·ml-1 for B. pertussis and 213 CFU·ml-1 for B. parapertussis The assay detected 16/18 unique B. pertussis/B. parapertussis strains. Of 71 potentially cross-reacting organisms, 5 generated false positives in 1/6 replicates; none of 6 additional Bordetella spp. were erroneously detected. Specimens were stable at 20 to 25°C for at least 10 h, at 4 to 8°C for 10 days, and at temperatures not exceeding -70°C for 6 months. Of 1,052 nasopharyngeal specimens from patients with suspected pertussis, 3.0% (n = 32) were B. pertussis positive and 0.2% (n = 2) were B. parapertussis positive. Combining these data with Aries Bordetella Assay data from 57 nasopharyngeal samples with previously confirmed B. pertussis or B. parapertussis data and with data from 50 contrived B. parapertussis samples, the proportions of positive and negative agreement of the respective Aries assays with the reference assays were 97.1% and 99.0% for B. pertussis and 100% and 99.7% for B. parapertussis The Aries Bordetella Assay provides accurate detection and distinction of B. pertussis and B. parapertussis infections within 2 h. (This study has been registered at ClinicalTrials.gov under registration no. NCT02862262.)

Cardiomyocyte-specific estrogen receptor alpha increases angiogenesis, lymphangiogenesis and reduces fibrosis in the female mouse heart post-myocardial infarction

Experimental studies showed that 17{beta}-estradiol (E2) and activated Estrogen Receptors (ER) protect the heart from ischemic injury. However, the underlying molecular mechanisms are not well understood. To investigate the role of ER{alpha} in cardiomyocytes in the setting of myocardial ischemia, we generated transgenic mice with cardiomyocyte-specific overexpression of ER-{alpha} (ER{alpha}-OE) and subjected them to Myocardial Infarction (MI). At the basal level, female and male ER{alpha}-OE mice showed increased Left Ventricular (LV) mass, LV volume and cardiomyocyte length. Two weeks after MI, LV volume was significantly increased and LV wall thickness decreased in female and male WT-mice and male ER{alpha}-OE, but not in female ER{alpha}-OE mice. ER{alpha}-OE enhanced expression of angiogenesis and lymphangiogenesis markers (Vegf, Lyve-1), and neovascularization in the peri-infarct area in both sexes. However, attenuated level of fibrosis and higher phosphorylation of JNK signaling pathway could be detected only in female ER{alpha}-OE after MI. In conclusion, our study indicates that ER{alpha} protects female mouse cardiomyocytes from the sequelae of ischemia through induction of neovascularization in a paracrine fashion and impaired fibrosis, which together may contribute to the attenuation of cardiac remodelling