6,117 research outputs found
Alterations in the mitochondrial proteome of neuroblastoma cells 2 in response to complex 1 inhibition
Increasing evidence points to mitochondrial dysfunction in Parkinson's disease (PD) associated with complex I dysfunction, but the exact pathways which lead to cell death have not been resolved. 2D-gel electrophoresis profiles of isolated mitochondria from neuroblastoma cells treated with subcytotoxic concentrations of l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP), a well-characterized complex I inhibitor, were assessed to identify associated targets. Up to 27 differentially expressed proteins were observed, of which 16 were identified using peptide mass fingerprinting. Changes in protein levels were validated by immunoprobing ID blots, confirming increases in heat shock cognate 71 kDa (Hsc70), 60 kDa heat shock protein (Hsp60), fumarase, glutamate oxaloacetate transaminase 2, ATP synthase subunit d, and voltage-dependent anion-channel 1 (VDACl). Immunoprobing of 2D blots revealed isoform changes in Hsc70, Hsp60, and VDACl. Subcytoxic concentrations of MPTP modulated a host of mitochondrial proteins including chaperones, metabolic enzymes, oxidative phosphorylation-related proteins, an inner mitochondrial protein (mitofilin), and an outer mitochondrial membrane protein (VDACl). Early changes in chaperones suggest a regulated link between complex 1 inhibition and protein folding. VDACl, a multifunctional protein, may have a key role in signaling between mitochondria and the rest of the cell prior to cell death. Our work provides new important information of relevance to PD
Surface doping in T6/ PDI-8CN2 Heterostructures investigated by transport and photoemission measurements
In this paper, we discuss the surface doping in sexithiophene (T6) organic
field-effect transistors by PDI-8CN2. We show that an accumulation
heterojunction is formed at the interface between the organic semiconductors
and that the consequent band bending in T6 caused by PDI-8CN2 deposition can be
addressed as the cause of the surface doping in T6 transistors. Several
evidences of this phenomenon have been furnished both by electrical transport
and photoemission measurements, namely the increase in the conductivity, the
shift of the threshold voltage and the shift of the T6 HOMO peak towards higher
binding energies.Comment: 5 pages, 5 figure
New excavations in the central and southern sectors of Aeclanum in 2019 (Comune di Mirabella Eclano, Provincia di Avellino, Regione Campania)
Endoscopic palliative management of esophageal and tracheal rupture
The increasing number of patients requiring intensive care and airway support has led to a growing recognition that significant short- and long-term morbidity may be associated with the use of artificial airways;
this is despite significant improvements
in the materials used in laryngeal
tubes, which aim to decrease the trauma
associated with long-term intubation.
We present the first case, to our knowledge,of huge, nontraumatic, esophageal perforation, widely communicating with the trachea, and which was treated successfully with double endoscopic stent placement
Single-Crystal Organic Charge-Transfer Interfaces probed using Schottky-Gated Heterostructures
Organic semiconductors based on small conjugated molecules generally behave
as insulators when undoped, but the hetero-interfaces of two such materials can
show electrical conductivity as large as in a metal. Although charge transfer
is commonly invoked to explain the phenomenon, the details of the process and
the nature of the interfacial charge carriers remain largely unexplored. Here
we use Schottky-gated heterostructures to probe the conducting layer at the
interface between rubrene and PDIF-CN2 single crystals. Gate-modulated
conductivity measurements demonstrate that interfacial transport is due to
electrons, whose mobility exhibits band-like behavior from room temperature to
~ 150 K, and remains as high as ~ 1 cm2V-1s-1 at 30 K for the best devices. The
electron density decreases linearly with decreasing temperature, an observation
that can be explained quantitatively based on the heterostructure band diagram.
These results elucidate the electronic structure of rubrene-PDIF-CN2 interfaces
and show the potential of Schottky-gated organic heterostructures for the
investigation of transport in molecular semiconductors.Comment: 37 pages, 9 Figures (including supplementary information
Ionic liquid crystals based on viologen dimers: tuning the mesomorphism by varying the conformational freedom of the ionic layer
We investigated the liquid crystal behaviour of newly synthesised bistriflimide salts of symmetric viologen dimers. A smectic A phase was observed for intermediate spacer lengths and for relatively long lateral alkyl chains. The systems were characterised by thermal analysis, polarised optical microscopy, X-ray scattering and solid-state NMR. An intermediate ordered smectic phase was also exhibited by the compounds (except for systems with very short lateral chains) consisting of molten layers of alkyl chains and partially ordered ionic layers. These results, relating to the mesomorphic behaviour of viologen salts, are qualitatively compared to those of the more common imidazolium salts, highlighting the importance of the conformational degrees of freedom of the anions and of the cationic core. It appears that fine tuning of the conformational degrees of freedom of the ionic layer is an important component of mesophase stabilisation
Very low bias stress in n-type organic single crystal transistors
Bias stress effects in n-channel organic field-effect transistors (OFETs) are
investigated using PDIF-CN2 single-crystal devices with Cytop gate dielectric,
both under vacuum and in ambient. We find that the amount of bias stress is
very small as compared to all (p-channel) OFETs reported in the literature.
Stressing the PDIF-CN2 devices by applying 80 V to the gate for up to a week
results in a decrease of the source drain current of only ~1% under vacuum and
~10% in air. This remarkable stability of the devices leads to characteristic
time constants, extracted by fitting the data with a stretched exponential -
that are \tau ~ 2\cdot10^9 s in air and \tau ~ 5\cdot10^9 s in vacuum -
approximately two orders of magnitude larger than the best values reported
previously for p-channel OFETs.Comment: Submitted to Applied Physics Letters; 14 pages, 3 figure
Early childhood infections, antistreptococcal and basal ganglia antibodies in adult ADHD: a preliminary study
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