Effects of mesenchymal stem cell therapy, in association with pharmacologically active microcarriers releasing VEGF, in an ischaemic stroke model in the rat

Few effective therapeutic interventions are available to limit brain damage and functional deficits after ischaemic stroke. Within this context, mesenchymal stem cell (MSC) therapy carries minimal risks while remaining efficacious through the secretion of trophic, protective, neurogenic and angiogenic factors. The limited survival rate of MSCs restricts their beneficial effects. The usefulness of a three-dimensional support, such as a pharmacologically active microcarrier (PAM), on the survival of MSCs during hypoxia has been shown in vitro, especially when the PAMs were loaded with vascular endothelial growth factor (VEGF). In the present study, the effect of MSCs attached to laminin-PAMs (LM-PAMs), releasing VEGF or not, was evaluated in vivo in a model of transient stroke. The parameters assessed were infarct volume, functional recovery and endogenous cellular reactions. LM-PAMs induced the expression of neuronal markers by MSCs both in vitro and in vivo. Moreover, the prolonged release of VEGF increased angiogenesis around the site of implantation of the LM-PAMs and facilitated the migration of immature neurons towards the ischaemic tissue. Nonetheless, MSCs/LM-PAMs-VEGF failed to improve sensorimotor functions. The use of LM-PAMs to convey MSCs and to deliver growth factors could be an effective strategy to repair the brain damage caused by a stroke

Synthesis and in vivo evaluation of new 2-nitroimidazole 18F-radiotracers for PET hypoxia imaging.

LDM-TEPNational audienc

Microdose study of 11C radiolabeled Donecopride, a multi-target-directed ligand for Alzheimer’s disease treatment

LDM-TEPInternational audienc

N-[18F]-FluoropropylJDTic for κ-opioid receptor PET imaging : Radiosynthesis, pre-clinical evaluation, and metabolic investigation in comparison with parent JDTic.

LDM TEPInternational audienc

[18F]Fludarabine-PET in a murine model of multiple myeloma

LDM TEPInternational audiencePurpose Multiple myeloma (MM) is a haematological malignancy that affects plasma cells in the bone marrow. Recently, [ 18 F]fludarabine has been introduced as an innovative PET radiotracer for imaging lymphoma. It demonstrated a great potential for accurate imaging of lymphopro-liferative disorders. With the goal to question the usefulness of [ 18 F]fludarabine-PET in other haematological diseases, an in vivo MM model was investigated. Methods RPMI8226-GFP-Luc MM cells expressing the green fluorescent protein (GFP) as well as the luciferase reporter (Luc) were derived from the parental RPMI8226 cells. They were injected subcutaneously into the flank of nude mice. Myeloma tumour growth was followed using bioluminescence-based imaging (BLI) and characterised by immunohistochemistry (IHC). The tumour specificity of [ 18 F]fludarabine was evaluated and compared to [ 18 F]FDG. Results The tumoural uptake of [ 18 F]FDG was greater than that of [ 18 F]fludarabine. However, the quantitative data extracted from IHC stainings were in better agreement with [ 18 F]fludara-bine, when compared to [ 18 F]FDG. The relationship between the tumoural uptake of [ 18 F]-labelled tracers and the BLI quantitative data was also in favour of [ 18 F]fludarabine

Evaluation of the specificity of [18F]fludarabine PET/CT in a xenograft model of follicular lymphoma: comparison with [18F]FDG and impact of rituximab therapy

LDM TEPInternational audienc

Un nouveau radiotracer pour l’imagerie TEP de l’hypoxie à fort contraste et délai ultra-court : validation pré-clinique dans une modèle de sarcome

LDM TEPNational audienc

CNS lymphoma imaging with [18F]fludarabine-PET: comparison with [18F]FDG in a human lymphoma xenograft model

CERVOXY/LDM TEPInternational audienc

Transport into the central nervous system of [18F]FLT and [18F]Fludarabine for brain tumors

LDM TEPInternational audienc

JDTic-based radiotracers for Kappa Opioid Receptor PET Imaging: Design, Synthesis, Pharmacology, Molecular Modeling Studies, Radiolabelling, in vivo Characterization and Metabolic Investigation

LDM-TEPInternational audienc