Tolerância à salinidade em feijão (Phaseolus vulgaris L)

One of the applications of the tissue culture technique in plant improvement is the identification of cell lines which show salinity tolerance. Several authors were able to obtain saline stress-tolerant cell lines and show that mechanisms of tolerance to salts have a strong correlation between this phenomenon and a high macromolecule concentration in plant tissues. Callus obtained from embrionic axis of Phaseolus vulgarís cv. IAC carioca in solid medium, supplemented with 0 to 60 mM NaCl, as the salt treatment, were used. Callus harvesting was done on the 13th day, when they were processed for relative growth, protein, proline content and peroxidase acivity. The results show both, a decrease of the relative growth and of protein content in response to the NaCl treatment, as compared to controls. However, there was a significant increase on the proline content and on the peroxidase activity.Uma das aplicações das técnicas da cultura de tecidos no melhoramento é a identificação de linhas de células que apresentam tolerância à salinidade. Vários autores obtiveram linhas de células tolerantes ao estresse salino; e estudo de mecanismos bioquímicos da tolerância a sais em plantas tem demonstrado altas correlações entre estes e o acúmulo de macromoléculas em tecido de plantas superiores. Para verificar essas correlações em feijão (Phaseolus vulgaris cv IAC carioca), calos oriundos de eixos embrionários foram cultivados em meio sólido, suplementado com NaCl nas concentrações de 0 a 60 mM. Após 13 dias de incubação, os calos foram coletados e analisados quanto ao crescimento relativo, teor de proteínas, teor de prolina e atividade da peroxidase. Os parâmetros analisados mostraram decréscimo no crescimento relativo e no de proteínas em resposta ao NaCl. Paralelamente, observou-se aumento significativo no conteúdo de prolina e atividade da enzima peroxidase

Divisão Racional de Terras: um Estudo de Caso em Londrina, PR

At present, the lands fragmentation in little parts for family interests is done by the try and mistakes method, in which, the technician try to delimit the areas in similar dimensions and proportions for each interested. They do it based in theier perception and experience. The aim of this paper was to define a conduct line for the treatment of questions referent to the lands division and verify if the fragmentation proposed in a rural property at Londrina, Paraná state, in Brazil, was fair, considering as a critery, the land suitability. The rational land division was done based at the land use capacity system. It was applied a relative value to each area portion in agreement with its capability. As the equivalent area value of the each fragment consider the land quality, the final results should be similar. However, there is a large diversity of territories. The datas show that the ten resultant areas of the division proposed for the rural imovel present variated proportions of the diferents classes. Like this, with the division proposed by the expert, it was obtained area fragments very diferents related to the land qualit

Impactos Nutricionais E Produção De Pimentão Submetido à Deficiência Hídrica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The sweet pepper is a solanaceous plant of American origin with large consumption in Brazil. In order to study the effects of water stress on the sweet pepper crop, an experiment was conducted in greenhouse. The treatments consisted of soil water content (100%, 85%, 70% and 55% of moisture in the soil field capacity) in a randomized block design with 6 replicates where the experimental units consisted of 16 plants. During the experiment (47, 62, 77 and 92 days after planting) levels of macronutrients (N, P, K, Ca, Mg and S) and micronutrients (B, Cu, Fe, Mn and Zn) present in each plant fraction and the dry mass of plants for the accumulation of these determination were determined. At harvest the number, width and length of fruits from each plant were obtained. Variables were subjected to Tukey test at 5% probability. The water content in the soil interfered significantly with productive variables. To the accumulation of nutrients in the dry matter (leaf + stem) the elements with higher absorption are ordered as follows: K> N> Ca> Mg> S> P and Fe>Mn> Zn> B> Cu. © 2016, Universidade Estadual Paulista - UNESP. All rights reserved.214724735CNPq, Conselho Nacional de Desenvolvimento Científico e TecnológicoConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Avaliação da qualidade de um fertilizante produzido por compostagem conjunta de materiais orgânicos e rochas moídas.

Ao longo de milhares de anos, diferentes povos realizaram uma agricultura baseada no manejo dos materiais disponíveis nas propriedades rurais. Uma das formas para se atingir essa condiçãoé a produção e uso de compostos orgânicos. Este trabalho tevecomo objetivo avaliar a qualidade docompostoorgânico produzido com fontes de nutrientesdeorigem regionalem uma propriedade rural.As matérias-primas utilizadas para a produção do fertilizanteforam cama de bovino, serragem, casca de café e pó de rochas. A compostagem foi iniciada com relação C:N entre 25 e 35 e terminada com relação C:N menor que 15. A realização da aeração do condicionadorfoi feita com um compostador e, a umidificação, com um tanque pipa.Asanálisesquímica, orgânica, biológica e sanitária indicam que o processo de compostagem foi realizado de forma correta. Com isso, o composto exibiu a maioria das garantias mínimas para ser enquadrado como fertilizante orgânico composto classe A, o que pode melhoraras condições físicas, químicas e biológicas do solo, aumentando a autonomia do agricultor e diminuindo sua dependência dos combustíveis fósseis

Acclimation to short-term low temperatures in two Eucalyptus globulus clones with contrasting drought resistance

We tested the hypothesis that Eucalyptus globulus Labill. genotypes that are more resistant to dry environments might also exhibit higher cold tolerances than drought-sensitive plants. The effect of low temperatures was evaluated in acclimated and unacclimated ramets of a drought-resistant clone (CN5) and a drought-sensitive clone (ST51) of E. globulus. We studied the plants’ response via leaf gas exchanges, leaf water and osmotic potentials, concentrations of soluble sugars, several antioxidant enzymes and leaf electrolyte leakage. Progressively lowering air temperatures (from 24/16 to 10/ 2 C, day/night) led to acclimation of both clones. Acclimated ramets exhibited higher photosynthetic rates, stomatal conductances and lower membrane relative injuries when compared to unacclimated ramets. Moreover, low temperatures led to significant increases of soluble sugars and antioxidant enzymes activity (glutathione reductase, ascorbate peroxidase and superoxide dismutases) of both clones in comparison to plants grown at control temperature (24/16 C). On the other hand, none of the clones, either acclimated or not, exhibited signs of photoinhibition under low temperatures and moderate light. The main differences in the responses to low temperatures between the two clones resulted mainly from differences in carbon metabolism, including a higher accumulation of soluble sugars in the drought-resistant clone CN5 as well as a higher capacity for osmotic regulation, as compared to the droughtsensitive clone ST51. Although membrane injury data suggested that both clones had the same inherent freezing tolerance before and after cold acclimation, the results also support the hypothesis that the droughtresistant clone had a greater cold tolerance at intermediate levels of acclimation than the drought-sensitive clone. A higher capacity to acclimate in a short period can allow a clone to maintain an undamaged leaf surface area along sudden frost events, increasing growt

Produção da amoreira-preta ‘Tupy’ sob diferentes épocas de poda.

A amora-preta é uma opção importante para fruticultura paranaense, porém não há informações a respeito do cultivo dessa frutífera nas condições subtropicais do Estado. O objetivo deste trabalho foi avaliar a influência da época de poda na produção da amoreira-preta ‘Tupy’. O trabalho foi realizado em um pomar comercial, conduzido em sistema agroecológico. O delineamento experimental foi em blocos ao acaso, com quatro blocos e seis tratamentos (podas quinzenais realizadas durante o inverno). Em cada parcela, constituída de três plantas úteis, foram coletados dados fenológicos, produtivos e físico-químicos no ciclo de produção 2008/09 e 2009/10. Podas efetuadas no início de julho são as mais indicadas e podas tardias podem prejudicar o desempenho produtivo das amoreiras-pretas ‘Tupy’ no oeste do Paraná