Snapshots in time: MicroCT scanning of pottery sherds determines early domestication of sorghum (Sorghum bicolor) in East Africa

MicroCT visualisations of organic inclusions within pottery sherds from Khashm el Girba 23 (KG23), Sudan, reveal domesticated sorghum (Sorghum bicolor subsp. bicolor) at c. 3700–2900 BCE. The percentage of non-shattering spikelet bases was c. 73% of identifiable visualizations, with c. 27% representing wild types. These analyses demonstrate the domestication of sorghum is significantly earlier than suggested by previous archaeological research. These results also demonstrate that microCT scanning is a major qualitative and quantitative advance on pre-existing methods for the investigation of crop remains in pottery sherds, which hitherto have been reliant on surface impressions; it is non-destructive, provides higher resolution 3D imaging of organic inclusions, and enables greater archaeobotanical recovery of inclusions within a sherd. MicroCT analysis of ceramics, mudbrick and other building materials has considerable potential for improving the chronologies and resolution for the domestication of other cereals in the past

Evidence for Sorghum Domestication in Fourth Millennium BC Eastern Sudan: Spikelet Morphology from Ceramic Impressions of the Butana Group

Since the 1970s, the quest for finding the origins of domesticated sorghum in Africa has remained elusive despite the fact that sorghum (Sorghum bicolor (L.) Moench. sensu stricto) is one of the world’s most important cereals. Recognized as originating from wild populations in Africa (Sorghum arundinaceum (Desv.) Stapf), however, the date and cultural context of its domestication has been controversial, with many scholars inferring an early Holocene origin in parallel with better-known cereal domestications. This paper presents firm evidence that the process of domesticating sorghum was present in the far eastern Sahel in the southern Atbai at an archaeological site associated with the Butana Group. Ceramic sherds recovered from excavations undertaken by the Southern Methodist University Butana Project during the 1980s from the largest Butana Group site, KG23, near Kassala, eastern Sudan, were analyzed, and examination of the plant impressions in the pottery revealed diagnostic chaff in which both domesticated and wild sorghum types were identified, thus providing archaeobotanical evidence for the beginnings of cultivation and emergence of domesticated characteristics within sorghum during the fourth millennium BC in eastern Sudan

On the Origins and Dissemination of Domesticated Sorghum and Pearl Millet across Africa and into India: a View from the Butana Group of the Far Eastern Sahel

Four decades have passed since Harlan and Stemler (1976) proposed the eastern Sahelian zone as the most likely center of Sorghum bicolor domestication. Recently, new data on seed impressions on Butana Group pottery, from the fourth millennium BC in the southern Atbai region of the far eastern Sahelian Belt in Africa, show evidence for cultivation activities of sorghum displaying some domestication traits. Pennisetum glaucum may have been undergoing domestication shortly thereafter in the western Sahel, as finds of fully domesticated pearl millet are present in southeastern Mali by the second half of the third millennium BC, and present in eastern Sudan by the early second millennium BC. The dispersal of the latter to India took less than 1000 years according to present data. Here, we review the middle Holocene Sudanese archaeological data for the first time, to situate the origins and spread of these two native summer rainfall cereals in what is proposed to be their eastern Sahelian Sudan gateway to the Red Sea and the Indian Ocean trade

Expression of Distal-less, dachshund, and optomotor blind in Neanthes arenaceodentata (Annelida, Nereididae) does not support homology of appendage-forming mechanisms across the Bilateria

The similarity in the genetic regulation of arthropod and vertebrate appendage formation has been interpreted as the product of a plesiomorphic gene network that was primitively involved in bilaterian appendage development and co-opted to build appendages (in modern phyla) that are not historically related as structures. Data from lophotrochozoans are needed to clarify the pervasiveness of plesiomorphic appendage forming mechanisms. We assayed the expression of three arthropod and vertebrate limb gene orthologs, Distal-less (Dll), dachshund (dac), and optomotor blind (omb), in direct-developing juveniles of the polychaete Neanthes arenaceodentata. Parapodial Dll expression marks premorphogenetic notopodia and neuropodia, becoming restricted to the bases of notopodial cirri and to ventral portions of neuropodia. In outgrowing cephalic appendages, Dll activity is primarily restricted to proximal domains. Dll expression is also prominent in the brain. dac expression occurs in the brain, nerve cord ganglia, a pair of pharyngeal ganglia, presumed interneurons linking a pair of segmental nerves, and in newly differentiating mesoderm. Domains of omb expression include the brain, nerve cord ganglia, one pair of anterior cirri, presumed precursors of dorsal musculature, and the same pharyngeal ganglia and presumed interneurons that express dac. Contrary to their roles in outgrowing arthropod and vertebrate appendages, Dll, dac, and omb lack comparable expression in Neanthes appendages, implying independent evolution of annelid appendage development. We infer that parapodia and arthropodia are not structurally or mechanistically homologous (but their primordia might be), that Dll’s ancestral bilaterian function was in sensory and central nervous system differentiation, and that locomotory appendages possibly evolved from sensory outgrowths

Outbreak of Pneumonia in the Setting of Fatal Pneumococcal Meningitis among US Army Trainees: Potential Role of Chlamydia pneumoniae Infection

<p>Abstract</p> <p>Background</p> <p>Compared to the civilian population, military trainees are often at increased risk for respiratory infections. We investigated an outbreak of radiologically-confirmed pneumonia that was recognized after 2 fatal cases of serotype 7F pneumococcal meningitis were reported in a 303-person military trainee company (Alpha Company).</p> <p>Methods</p> <p>We reviewed surveillance data on pneumonia and febrile respiratory illness at the training facility; conducted chart reviews for cases of radiologically-confirmed pneumonia; and administered surveys and collected nasopharyngeal swabs from trainees in the outbreak battalion (Alpha and Hotel Companies), associated training staff, and trainees newly joining the battalion.</p> <p>Results</p> <p>Among Alpha and Hotel Company trainees, the average weekly attack rates of radiologically-confirmed pneumonia were 1.4% and 1.2% (most other companies at FLW: 0-0.4%). The pneumococcal carriage rate among all Alpha Company trainees was 15% with a predominance of serotypes 7F and 3. <it>Chlamydia pneumoniae </it>was identified from 31% of specimens collected from Alpha Company trainees with respiratory symptoms.</p> <p>Conclusion</p> <p>Although the etiology of the outbreak remains unclear, the identification of both <it>S. pneumoniae </it>and <it>C. pneumoniae </it>among trainees suggests that both pathogens may have contributed either independently or as cofactors to the observed increased incidence of pneumonia in the outbreak battalion and should be considered as possible etiologies in outbreaks of pneumonia in the military population.</p

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection

2000 Ohio-Grape Wine Short Course

Air, water, sun, and fire--the cooper's footprint on the barrel / Henry Work -- Keeping the bugs unhappy; successful barrel sanitation and maintenance / Henry Work -- Recommended methods for cleaning and maintaining oak cooperage / Phil Burton and Henry Work, with Jim Yerkes -- Chip me, stave me, oak me! The romance, dollars and sense of barrel alternatives / Tim DiPlacido -- Oak experiments / Roland Riesen -- Barrel experiment / Nick Ferrante -- Exploring the versatility and potential of vidal / Roland Riesen -- Ferrante: 1999 vineyard planting / Nick Ferrante -- Breeding rootstocks for current and impending viticultural problems / Andrew Walker -- Grape expectations looking toward traditional and non-traditional sponsors to enhance your event / Doniella Winchell -- Assessing grape maturity by taste and by numbers / Thomas Henick-Kling -- Influence of fruit condition on wine quality / James F. Gallander -- Influence of post bottling storage temperature and SO2 on wine quality / T. E. Steiner -- What we do at harvest to help wine quality / Tony Debevc -- Delivering wine quality / Nick Ferrante -- Criteria for selecting rootstocks / Andrew Walker -- A comparison of Pinot noir production in New York and Burgundy / Pascal Durand and Leslie Weston -- A unique approach to harvest labor / Fran Massaro -- New fungicide registrations for grapes in the year 2000 / Michael Ellis -- Studies to determine time of susceptibility of grape berry and rachis tissues to infection by Phomopsis viticola / O. Erincik, L. V. Madden, D. C. Ferree and M. A. Ellis -- Rootstock performance in Ohio / Arnie Esterer -- Growing your own: vinifera grafting experiments (1999) / Ron Barrett -- Developing an effective fungicide spray program for wine grapes in Ohio / Michael Ellis -- Light and fruit set / David C. Ferree, David M. Scurlock and John C. Schmid -- Soil amendments and mulches in tree health management / Harry Hoitink, Matthew Krause and Randy Zondag -- Report of 5th International Symposium on Cool Climate Viticulture and Enology / Roland Riesen -- Control strategies for soil insects in the vineyard / Roger Williams and Dan Fickl

Measuring the Quality of Observational Study Data in an International HIV Research Network

Observational studies of health conditions and outcomes often combine clinical care data from many sites without explicitly assessing the accuracy and completeness of these data. In order to improve the quality of data in an international multi-site observational cohort of HIV-infected patients, the authors conducted on-site, Good Clinical Practice-based audits of the clinical care datasets submitted by participating HIV clinics. Discrepancies between data submitted for research and data in the clinical records were categorized using the audit codes published by the European Organization for the Research and Treatment of Cancer. Five of seven sites had error rates >10% in key study variables, notably laboratory data, weight measurements, and antiretroviral medications. All sites had significant discrepancies in medication start and stop dates. Clinical care data, particularly antiretroviral regimens and associated dates, are prone to substantial error. Verifying data against source documents through audits will improve the quality of databases and research and can be a technique for retraining staff responsible for clinical data collection. The authors recommend that all participants in observational cohorts use data audits to assess and improve the quality of data and to guide future data collection and abstraction efforts at the point of care