Linewidths in bound state resonances for helium scattering from Si(111)-(1x1)H

Helium-3 spin-echo measurements of resonant scattering from the Si(111)–(1 × 1)H surface, in the energy range 4–14 meV, are presented. The measurements have high energy resolution yet they reveal bound state resonance features with uniformly broad linewidths. We show that exact quantum mechanical calculations of the elastic scattering, using the existing potential for the helium/Si(111)–(1 × 1)H interaction, cannot reproduce the linewidths seen in the experiment. Further calculations rule out inelastic and other mechanisms that might give rise to losses from the elastic scattering channels. We show that corrugation in the attractive part of the atom–surface potential is the most likely origin of the experimental lineshapes

Comments on ``The first detections of the Extragalactic Background Light at 3000, 5500, and 8000 A'' by Bernstein, Freedman and Madore

A critical discussion is presented of the data analysis applied by Bernstein, Freedman and Madore (2002 ApJ, 571, 56; and ApJ 571, 85) in their measurement of the Extragalactic Background Light. There are questionable assumptions in the analysis of the ground-based observations of the Zodiacal Light. The modeling of the Diffuse Galactic Light is based on an underestimated value of the dust column density along the line of sight. Comparison with the previously presented results from the same observations reveals a puzzling situation: in spite of a large difference in the atmospheric scattered light corrections the derived Extragalactic Background Light values are exactly the same. The claim of the paper of a ``detection of the Extragalactic Background Light'' appears premature.Comment: 6 pages, accepted for Ap

TGF-β1 enhances cardiomyogenic differentiation of skeletal muscle-derived adult primitive cells

The optimal medium for cardiac differentiation of adult primitive cells remains to be established. We quantitatively compared the efficacy of IGF-1, dynorphin B, insulin, oxytocin, bFGF, and TGF-beta1 in inducing cardiomyogenic differentiation. Adult mouse skeletal muscle-derived Sca1+/CD45-/c-kit-/Thy-1+ (SM+) and Sca1-/CD45-/c-kit-/Thy-1+ (SM-) cells were cultured in basic medium (BM; DMEM, FBS, IGF-1, dynorphin B) alone and BM supplemented with insulin, oxytocin, bFGF, or TGF-beta1. Cardiac differentiation was evaluated by the expression of cardiac-specific markers at the mRNA (qRT-PCR) and protein (immunocytochemistry) levels. BM+TGF-beta1 upregulated mRNA expression of Nkx2.5 and GATA-4 after 4 days and Myl2 after 9 days. After 30 days, BM+TGF-beta1 induced the greatest extent of cardiac differentiation (by morphology and expression of cardiac markers) in SM- cells. We conclude that TGF-beta1 enhances cardiomyogenic differentiation in skeletal muscle-derived adult primitive cells. This strategy may be utilized to induce cardiac differentiation as well as to examine the cardiomyogenic potential of adult tissue-derived stem/progenitor cells

Porosity microstructures of a sandstone affected by a normal fault

Dans un système de failles normales de la bordure du fossé rhénan, les interactions eaux-roches de part et d’autre de ces failles peuvent contrôler les conditions des circulations fluides. L’objectif de ce travail est de caractériser les structures du réseau poreux dans la zone endommagée autour d’une de ces failles. Il est intéressant d’étudier la relation entre porosité et perméabilité dans cette zone. Des études pétrographiques et pétrophysiques, des mesures microthermométriques sur des inclusions fluides et la composition isotopique de l’oxygène ont permis de caractériser les structures de porosité des roches et notamment des ciments primaires et secondaires. Le couplage de ces approches montre qu’une faille normale peut à la fois jouer le rôle de drain et de barrière à la circulation des fluides. En fonction de la direction de circulation, la faille joue le rôle de drain en laissant remonter les fluides parallèlement au plan de faille et le rôle de barrière, en focalisant les circulations dans le toit. L’anisotropie, notamment des propriétés de transfert héritées des conditions de dépôts fluviatiles, est profondément modifiée par les transferts subits dans le matériau. Ainsi les modifications des transferts dépendent des modifications du réseau poreux : l’hétérogénéité de la structure du réseau et l’anisotropie d’orientation ou de connectivité. Ce modèle de circulation est contrôlé par une interaction entre les modifications des structures du réseau poreux et les circulations fluides, entraînant des modifications de l’anisotropie de certaines propriétés du matériau autour de la faille

The epochs of early-type galaxy formation as a function of environment

The aim of this paper is to set constraints of the epochs of early-type galaxy formation through the 'archaeology' of the stellar populations in local galaxies. Using our models of absorption line indices that account for variable abundance ratios, we derive the stellar population parameters of 124 early-type galaxies in high and low density environments. We find that all three parameters age, metallicity, and alpha/Fe ratio are correlated with velocity dispersion. We further find evidence for an influence of the environment on the stellar population properties. Massive early-type galaxies in low-density environments appear on average ~2 Gyrs younger and slightly more metal-rich than their counterparts in high density environments. No offsets in the alpha/Fe ratios, instead, are detected. We translate the derived ages and alpha/Fe ratios into star formation histories. We show that most star formation activity in early-type galaxies is expected to have happened between redshifts 3 and 5 in high density and between redshifts 1 and 2 in low density environments. We conclude that at least 50 per cent of the total stellar mass density must have already formed at z 1, in good agreement with observational estimates of the total stellar mass density as a function of redshift. Our results suggest that significant mass growth in the early-type galaxy population below z 1 must be restricted to less massive objects, and a significant increase of the stellar mass density between redshifts 1 and 2 should be present caused mainly by the field galaxy population. The results of this paper further imply vigorous star formation episodes in massive objects at z 2-5 and the presence of evolved ellipticals around z 1, both observationally identified as SCUBA galaxies and EROs.Comment: 20 pages, 10 figures, plus appendix, accepted by Ap

Visualization and quantitation of the expression of microRNAs and their target genes in neuroblastoma single cells using imaging cytometry

<p>Abstract</p> <p>Background</p> <p>MicroRNAs (miRNAs) are regulatory molecules that play an important role in many physiological processes, including cell growth, differentiation, and apoptosis. In addition to modulating normal cellular functions, it has also been reported that miRNAs are involved in the development of many pathologies, including cardiovascular diseases, cancer, inflammation, and neurodegeneration. Methods for the sensitive detection and measurement of specific miRNAs and their cellular targets are essential for both basic research endeavours, as well as diagnostic efforts aimed at understanding the role of miRNAs in disease processes.</p> <p>Findings</p> <p>In this study, we describe a novel, imaging cytometry-based protocol that allows for simultaneous visualisation and quantification of miRNAs and their putative targets. We validated this methodology in a neuronal cell line by examining the relationship of the miRNA miR-124 and its known target, cyclin dependent kinase 6 (CDK6). We found that ectopic overexpression of miR-124 resulted in the downregulation of CDK6, decreased cellular proliferation, and induced cellular morphological changes.</p> <p>Conclusions</p> <p>This method is suitable for analysing the expression and cellular localisation of miRNAs and target proteins in small cell subsets within a heterogeneous cell suspension. We believe that our cytometry-based methodology will be easily adaptable to miRNA studies in many areas of biomedical research including neuroscience, stem cell biology, immunology, and oncology.</p

Nuclear stellar discs in low-luminosity elliptical galaxies: NGC 4458 and NGC 4478

We present the detection of nuclear stellar discs in the low-luminosity elliptical galaxies NGC 4458 and NGC 4478, which are known to host a kinematically-decoupled core. Using archival HST imaging, and available absorption line-strength index data based on ground-based spectroscopy, we investigate the photometric parameters and the properties of the stellar populations of these central structures. Their scale length, h, and face-on central surface brightness, mu_0^c, fit on mu_0^c-h relation for galaxy discs. For NGC 4458 these parameters are typical for nuclear discs, while the same quantities for NGC 4478 lie between those of nuclear discs and the discs of discy ellipticals. We present Lick/IDS absorption line-strength measurements of Hbeta, Mgb, along the major and minor axes of the galaxies. We model these data with simple stellar populations that account for the alpha/Fe overabundance. The counter-rotating central disc of NGC 4458 is found to have similar properties to the decoupled cores of bright ellipticals. This galaxy has been found to be uniformly old despite being counter-rotating. In contrast, the cold central disc of NGC 4478 is younger, richer in metals and less overabundant than the main body of the galaxy. This points to a prolonged star formation history, typical of an undisturbed disc-like, gas-rich (possibly pre-enriched) structure.Comment: 11 pages, 8 figures, accepted for pubblication on MNRA

Face-on galaxies NGC 524 and NGC 6340: chemically decoupled nuclei and inclined circumnuclear disks

Central regions of the early-type disk galaxies NGC 524 and NGC 6340 have been investigated with the Multi-Pupil Field Spectrograph at the 6m telescope of the Special Astrophysical Observatory of the Russian Academy of Sciences. I confirm the existence of chemically distinct stellar nuclei in these galaxies which have been claimed earlier. The metallicity differences which are found between the nuclei and the bulges, 0.5 - 1.0 kpc from the centers, reach 0.5 - 0.6 dex. Both nuclei are magnesium overabundant, but the bulges have different magnesium-to-iron ratios: it is solar in NGC 6340 and the same as the nuclear one in NGC 524. The kinematical and morphological analyses reveal the existence of inclined central disks in these galaxies. In NGC 524 the central disk consists of stars, dust, and ionized gas; its extension may be as large as up to R=3 kpc, and it is inclined by more than 20 deg to the global galactic plane. In NGC 6340 only a gaseous polar disk with the radius less than 500 pc is detected.Comment: 25 pages, 15 figures, to appear in Astronomical Journal, August2000 issu

Very small embryonic-like stem cells (VSELs) represent a real challenge in stem cell biology : recent pros and cons in the midst of a lively debate

The concept that adult tissue, including bone marrow (BM), contains early-development cells with broader differentiation potential has again been recently challenged. In response, we would like to review the accumulated evidence from several independent laboratories that adult tissues, including BM, harbor a population of very rare stem cells that may cross germ layers in their differentiation potential. Thus, the BM stem cell compartment hierarchy needs to be revisited. These dormant, early-development cells that our group described as very small embryonic-like stem cells (VSELs) most likely overlap with similar populations of stem cells that have been identified in adult tissues by other investigators as the result of various experimental strategies and have been given various names. As reported, murine VSELs have some pluripotent stem cell characteristics. Moreover, they display several epiblast/germline markers that suggest their embryonic origin and developmental deposition in adult BM. Moreover, at the molecular level, changes in expression of parentally imprinted genes (for example, Igf2–H19) and resistance to insulin/insulin-like growth factor signaling (IIS) regulates their quiescent state in adult tissues. In several emergency situations related to organ damage, VSELs can be activated and mobilized into peripheral blood, and in appropriate animal models they contribute to tissue organ/regeneration. Interestingly, their number correlates with lifespan in mice, and they may also be involved in some malignancies. VSELs have been successfully isolated in several laboratories; however, some investigators experience problems with their isolation