Binding branched and linear DNA structures: from isolated clusters to fully bonded gels

The proper design of DNA sequences allows for the formation of well defined supramolecular units with controlled interactions via a consecution of self-assembling processes. Here, we benefit from the controlled DNA self-assembly to experimentally realize particles with well defined valence, namely tetravalent nanostars (A) and bivalent chains (B). We specifically focus on the case in which A particles can only bind to B particles, via appropriately designed sticky-end sequences. Hence AA and BB bonds are not allowed. Such a binary mixture system reproduces with DNA-based particles the physics of poly-functional condensation, with an exquisite control over the bonding process, tuned by the ratio, r, between B and A units and by the temperature, T. We report dynamic light scattering experiments in a window of Ts ranging from 10{\deg}C to 55{\deg}C and an interval of r around the percolation transition to quantify the decay of the density correlation for the different cases. At low T, when all possible bonds are formed, the system behaves as a fully bonded network, as a percolating gel and as a cluster fluid depending on the selected r.Comment: 15 pages, 11 figure

Evidence of a Metal Rich Galactic Bar from the Vertex Deviation of the Velocity Ellipsoid

We combine radial velocities, proper motions, and low resolution abundances for a sample of 315 K and M giants in the Baade's Window (l,b)=(0.9,-4) Galactic bulge field. The velocity ellipsoid of stars with [Fe/H]>-0.5 dex shows a vertex deviation in the plot of radial versus transverse velocity, consistent with that expected from a population with orbits supporting a bar. We demonstrate that the significance of this vertex deviation using non-parametric rank correlation statistic is >99%. The velocity ellipsoid for the metal poor ([FeH]<-0.5) part of the population shows no vertex deviation and is consistent with an isotropic, oblate rotating population. We find no evidence for kinematic subgroups, but there is a mild tendency for the vertical velocity dispersion sigma_b to decrease with increasing metallicity.Comment: 4 pages, ApJ Letters, submitte

Drivers of long-distance spotting during wildfires in south-eastern Australia

We analysed the influence of wildfire area, topography, fuel, surface weather and upper-level weather conditions on long-distance spotting during wildfires. The analysis was based on a large dataset of 338 observations, from aircraft-acquired optical line scans, of spotting wildfires in south-east Australia between 2002 and 2018. Source fire area (a measure of fire activity) was the most important predictor of maximum spotting distance and the number of long-distance spot fires produced (i.e. >500 m from a source fire). Weather (surface and upper-level), vegetation and topographic variables had important secondary effects. Spotting distance and number of long-distance spot fires increased strongly with increasing source fire area, particularly under strong winds and in areas containing dense forest and steep slopes. General vegetation descriptors better predicted spotting compared with bark hazard and presence variables, suggesting systems that measure and map bark spotting potential need improvement. The results from this study have important implications for the development of predictive spotting and wildfire behaviour models

Analysis of variation in distance, number, and distribution of spotting in southeast Australian wildfires

Spotting during wildfires can significantly influence the way wildfires spread and reduce the chances of successful containment by fire crews. However, there is little published empirical evidence of the phenomenon. In this study, we have analysed spotting patterns observed from 251 wildfires from a database of over 8000 aerial line scan images capturing active wildfire across mainland southeast Australia between 2002 and 2018. The images were used to measure spot fire numbers, number of “long-distance” spot fires (> 500 m), and maximum spotting distance. We describe three types of spotting distance distributions, compare patterns among different regions of southeast Australia, and associate these with broad measures of rainfall, elevation, and fuel type. We found a relatively high correlation between spotting distance and numbers; however, there were also several cases of wildfires with low spot fire numbers producing very long-distance spot fires. Most long-distance spotting was associated with a “multi-modal” distribution type, where high numbers of spot fires ignite close to the source fire and isolated or small clumps of spot fires ignite at longer distances. The multi-modal distribution suggests that current models of spotting distance, which typically follow an exponential-shaped distribution, could underestimate long-distance spotting. We also found considerable regional variation in spotting phenomena that may be associated with significant variation in rainfall, topographic ruggedness, and fuel descriptors. East Victoria was the most spot-fire-prone of the regions, particularly in terms of long-distance spotting

Testosterone enables growth and hypertrophy in fusion impaired myoblasts that display myotube atrophy: deciphering the role of androgen and IGF-I receptors

We have previously highlighted the ability of testosterone to improve differentiation and myotube hypertrophy in fusion impaired myoblasts that display reduced myotube hypertrophy at 72hrs (after transfer to low serum media) via multiple population doublings (PD) vs. their parental controls (CON); an observation which is abrogated via PI3K/Akt inhibition (Deane et al. 2013). However, whether the most predominant molecular mechanism responsible for T induced hypertrophy occurs directly via androgen receptor or indirectly via IGF-IR/PI3K/Akt pathway is currently debated. PD and CON C2C12 muscle cells were exposed to low serum conditions in the presence or absence of T (100 nM) ± inhibitors of AR (flutamide/F, 40 μm) and IGF-IR (Picropodophyllin/PPP, 150 nM) for 72 hrs and 7 days (early/late muscle differentiation respectively). T increased AR and Akt abundance, myogenin expression, and myotube hypertrophy, but not ERK1/2 activity in both CON and PD cell types. Akt activity was not increased significantly in either cell type with T. Testosterone was unable to promote early differentiation in the presence of IGF-IR inhibitor (PPP) yet still able to promote appropriate later increases in myotube hypertrophy and AR abundance despite IGF-IR inhibition. The addition of the AR inhibitor powerfully attenuated all T induced increases in differentiation and myotube hypertrophy with corresponding reductions in AR abundance, phosphorylated Akt, ERK1/2 and gene expression of IGF-I, myoD and myogenin with increases in myostatin mRNA both cell types. Interestingly, despite basally reduced differentiation and myotube hypertrophy, PD cells showed larger increased in AR abundance vs. CON cells, a response abrogated in the presence of AR but not IGF-IR inhibitors. Furthermore, T induced increases in Akt abundance were sustained despite the presence of IGF-IR inhibition in PD cells only. However, flutamide alone reduced IGF-IR mRNA in both cell types across time points, with an observed reduction in activity of ERK and Akt, perhaps suggesting that IGF-IR was transcriptionally regulated by AR. However, where testosterone increased AR protein content there was no increases observed in IGF-IR gene expression. Overall, this suggested that sufficient AR was important to enable normal gene expression of IGF-IR and downstream signalling, yet elevated levels of AR due to testosterone had no further effect on IGF-IR, despite testosterone increasing Akt abundance in the presence of IGF-IR inhibitor. In conclusion, testosterones ability to improve differentiation and myotube hypertrophy occurred predominately via increases in AR and Akt abundance in both CON and PD cells, with fusion impaired cells (PD) showing an increased responsiveness to T induced AR levels. Finally, T induced increases in myotube hypertrophy (but not early differentiation) occurred independently of upstream IGF-IR input, however it appears that normal AR function in basal conditions is required for adequate IGF-IR gene expression and downstream Akt abundance

Multivariate spatial smoothing using additive regression splines

We describe additive regression spline models as tools for smooth interpolation of fields that depend on several variables in a spatially varying way. Additive regression models can bypass the usual technical difficulties associated with the curse of dimension. We formulate the additive regression spline minimisation problem and prove that this problem is uniquely solvable under suitable conditions on the data. The resulting additive regression spline may be seen as a special case of general additive tensor product splines. Moreover, we show that additive regression splines may be implemented by a relatively straightforward extension of the methods used in the implementation of standard thin plate splines. The performance of additive regression splines is demonstrated on a simulated noisy data set

Galaxy Orbits for Galaxy Clusters in Sloan Digital Sky Survey and 2dF Galaxy Redshift Survey

We present the results of a study for galaxy orbits in galaxy clusters using a spectroscopic sample of galaxies in Sloan Digital Sky Survey (SDSS) and 2dF Galaxy Redshift Survey (2dFGRS). We have determined the member galaxies of Abell clusters covered by these surveys using the galaxies' redshift and positional data. We have selected 10 clusters using three criteria: the number of member galaxies is greater than or equal to 40, the spatial coverage is complete, and X-ray mass profile is available in the literature. We derive the radial profile of the galaxy number density and velocity dispersion using all, early-type, and late-type galaxies for each cluster. We have investigated the galaxy orbits for our sample clusters with constant and variable velocity anisotropies over the clustercentric distance using Jeans equation. Using all member galaxies, the galaxy orbits are found to be isotropic within the uncertainty for most of sample clusters, although it is difficult to conclude strongly for some clusters due the large errors and the variation as a function of the clustercentric distance in the calculated velocity anisotropies. We investigated the orbital difference between early-type and late-type galaxies for four sample clusters, and found no significant difference between them.Comment: 59 pages, 21 figures. To appear in ApJ. Paper with high resolution figures are available at http://astro.kias.re.kr/~hshwang/papers/orbit.pd

Risk factors for human brucellosis in northern Tanzania

Little is known about the epidemiology of human brucellosis in sub-Saharan Africa. This hampers prevention and control efforts at the individual and population levels. To evaluate risk factors for brucellosis in northern Tanzania, we conducted a study of patients presenting with fever to two hospitals in Moshi, Tanzania. Serum taken at enrollment and at 4–6 week follow-up was tested by Brucella microagglutination test. Among participants with a clinically compatible illness, confirmed brucellosis cases were defined as having a ≥ 4-fold rise in agglutination titer between paired sera or a blood culture positive for Brucella spp., and probable brucellosis cases were defined as having a single reciprocal titer ≥ 160. Controls had reciprocal titers &lt; 20 in paired sera. We collected demographic and clinical information and administered a risk factor questionnaire. Of 562 participants in the analysis, 50 (8.9%) had confirmed or probable brucellosis. Multivariable analysis showed that risk factors for brucellosis included assisting goat or sheep births (Odds ratio [OR] 5.9, 95% confidence interval [CI] 1.4, 24.6) and having contact with cattle (OR 1.2, 95% CI 1.0, 1.4). Consuming boiled or pasteurized dairy products was protective against brucellosis (OR 0.12, 95% CI 0.02, 0.93). No participants received a clinical diagnosis of brucellosis from their healthcare providers. The under-recognition of brucellosis by healthcare workers could be addressed with clinician education and better access to brucellosis diagnostic tests. Interventions focused on protecting livestock keepers, especially those who assist goat or sheep births, are needed

AN ALTERNATIVE METHOD TO THAT OF THE R6 PROCEDURE FOR THE TREATMENT OF COMBINED PRIMARY AND SECONDARY LOADING

ABSTRACT An alternative definition for the assessment point parameter of the R6 defect assessment procedure is proposed, for combined primary and secondary loading. This alternative definition removes the requirement to calculate a plasticity correction factor for secondary stresses, ρ (or V), used in the conventional R6 definition of . To compare these definitions, both the R6 procedure and the alternative method are presented as Crack Driving Force (CDF) estimation schemes. The required inputs to these estimation schemes have been determined from finite element analyses, for the particular case, of a thick-walled cylinder with a fully circumferential internal defect and subjected to internal pressure and a radial through-wall temperature gradient. Comparisons of CDF estimates with those determined from full inelastic finite element analyses have shown, for the cases studied, that both the R6 and alternative approaches provide conservative estimates of CDF compared to those obtained from finite element analyses, with the degree of conservatism far greater for the conventional R6 approach. Further finite element validation with different geometries, loadings and material properties is required before the alternative procedure could be considered for inclusion in the R6 defect assessment procedure as an alternative to the procedure of the main section of the document