Efficiency of Single Nucleotide Polymorphisms to improve a genetic map of complex pedigree grapevines

A set of 47 SNP (single nucleotide polymorphisms) markers (Cabezas et al. 2011) was tested for their usefulness to improve a genetic map from the cross of GF.GA‑52-42 x 'Solaris' previously established with SSR markers (Schwander et al. 2012). 55.3 % of the SNPs showed informative segregation and 26 SNP markers were localized on 16 of the 19 linkage groups of grapevine. Five chromosome regions with large gaps of recombining SSR markers could be equipped by positioning a SNP marker there. One SNP marker, VV10992, was found linked to the major resistance locus Rpv10 and should be applicable for marker-assisted selection

Grapevine breeding under climate change: Applicability of a molecular marker linked to véraison

Viticulture is of high economic value. Traditional grapevine cultivars (Vitis vinifera L.) are used in adaptation to the climatic conditions from Northern to Southern European growing areas. However, the recent trend of global warming causes quality deficits due to a shift of the plant's development to earlier times in the year. As a consequence, premature ripening happens under warm temperatures and interferes with the balanced accumulation of sugars, acids, aroma profiles and berry coloration. Modern grapevine breeding is challenged to include the ripening behavior as an important trait (besides pathogen resistance and other characteristics) into the development of novel cultivars well suited for sustainable viticulture. Breeders nowadays apply molecular markers to follow the introgression of desired traits on the genetic level. Previous work has identified a molecular tag on grapevine chromosome 16 strongly linked to the time of véraison, the start of the second phase of berry ripening, in a segregating cross population. In this study we analyzed the transferability of this marker to a set of 36 grapevine cultivars commonly used in German viticulture. Association analysis verified the predictive character of the time point of véraison for maturation time and confirmed the diagnostic potential of the véraison-linked marker in this extended set of cultivars

A view into American grapevine history: Vitis vinifera cv. 'Sémillon' is an ancestor of 'Catawba' and 'Concord'

The Vitis vinifera background of 'Catawba' and 'Concord' was investigated by using SSR analysis: 'Sémillon' was shown to be an ancestor of 'Catawba', while the wild parent remains unknown. 'Concord' was confirmed to be an offspring of 'Catawba' and another unknown wild parent. Since these two important American varieties most likely resulted from random natural crosses and successful selection, the original, wild growing wild donors remain unknown

Corrigendum to "High-resolution interpolar difference of atmospheric methane around the Last Glacial Maximum" published in Biogeosciences, 9, 3961–3977, 2012

No abstract available

Multi-omics insights into the biological mechanisms underlying statistical gene-by-lifestyle interactions with smoking and alcohol consumption

Though both genetic and lifestyle factors are known to influence cardiometabolic outcomes, less attention has been given to whether lifestyle exposures can alter the association between a genetic variant and these outcomes. The Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Consortium\u27s Gene-Lifestyle Interactions Working Group has recently published investigations of genome-wide gene-environment interactions in large multi-ancestry meta-analyses with a focus on cigarette smoking and alcohol consumption as lifestyle factors and blood pressure and serum lipids as outcomes. Further description of the biological mechanisms underlying these statistical interactions would represent a significant advance in our understanding of gene-environment interactions, yet accessing and harmonizing individual-level genetic and \u27omics data is challenging. Here, we demonstrate the coordinated use of summary-level data for gene-lifestyle interaction associations on up to 600,000 individuals, differential methylation data, and gene expression data for the characterization and prioritization of loci for future follow-up analyses. Using this approach, we identify 48 genes for which there are multiple sources of functional support for the identified gene-lifestyle interaction. We also identified five genes for which differential expression was observed by the same lifestyle factor for which a gene-lifestyle interaction was found. For instance, in gene-lifestyle interaction analysis, the T allele of rs6490056

Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men.

We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic response of the human organism to the ingestion of food is dependent on metabolic health. The randomized crossover study included seven normal weight and seven obese men consuming three doses (500/1000/1500 kcal) of a high-fat meal. The blood cell transcriptome was measured before and 2, 4, and 6 h after meal ingestion (168 samples). We applied univariate and multivariate statistics to investigate differentially expressed genes in both study groups. We identified 624 probe sets that were up- or down-regulated after the caloric challenge in a dose-dependent manner. These transcripts were most responsive to the 1500 kcal challenge in the obese group and were associated with postprandial insulin and oxidative phosphorylation. Furthermore, the data revealed a separation of the obese group into individuals whose response was close to the normal weight group and individuals with a transcriptional response indicative of a loss of metabolic flexibility. The molecular signature provided by the postprandial transcriptomic response of blood cells to increasing caloric doses of a high-fat meal challenge may represent a sensitive way to evaluate the qualitative impact of food on human health

Power-gated MOS current mode logic (PG-MCML): a power aware DPA-resistant standard cell library

MOS Current Mode Logic (MCML) is one of the most promising logic style to counteract power analysis attacks. Unfortunately, the static power consumption of MCML standard cells is significantly higher compared to equivalent functions implemented using static CMOS logic. As a result, the use of such a logic style is very limited in portable devices. Paradoxically, these devices are the most sensitive to physical attacks, thus the ones which would benefit more from the adoption of MCML

A 60 yr record of atmospheric carbon monoxide reconstructed from Greenland firn air

We present the first reconstruction of the Northern Hemisphere (NH) high latitude atmospheric carbon monoxide (CO) mole fraction from Greenland firn air. Firn air samples were collected at three deep ice core sites in Greenland (NGRIP in 2001, Summit in 2006 and NEEM in 2008). CO records from the three sites agree well with each other as well as with recent atmospheric measurements, indicating that CO is well preserved in the firn at these sites. CO atmospheric history was reconstructed back to the year 1950 from the measurements using a combination of two forward models of gas transport in firn and an inverse model. The reconstructed history suggests that Arctic CO in 1950 was 140–150 nmol mol-1, which is higher than today's values. CO mole fractions rose by 10–15 nmol mol-1 from 1950 to the 1970s and peaked in the 1970s or early 1980s, followed by a ˜ 30 nmol mol-1 decline to today's levels. We compare the CO history with the atmospheric histories of methane, light hydrocarbons, molecular hydrogen, CO stable isotopes and hydroxyl radicals (OH), as well as with published CO emission inventories and results of a historical run from a chemistry-transport model. We find that the reconstructed Greenland CO history cannot be reconciled with available emission inventories unless unrealistically large changes in OH are assumed. We argue that the available CO emission inventories strongly underestimate historical NH emissions, and fail to capture the emission decline starting in the late 1970s, which was most likely due to reduced emissions from road transportation in North America and Europe