49 research outputs found
Evaporation kinetics in swollen porous polymeric networks
Ponencia presentada en el Congreso Euromar 2014.Fil: Velasco, Manuel Isaac. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Silletta, Emilia Victoria. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Monti, Gustavo Alberto. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Acosta, Rodolfo HĂ©ctor. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Velasco, Manuel Isaac. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Silletta, Emilia Victoria. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Monti, Gustavo Alberto. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Acosta, Rodolfo HĂ©ctor. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Gomez, Cesar Gerardo. Universidad Nacional de CĂłrdoba. Facultad de Ciencias QuĂmicas. Departamento de QuĂmica OrgĂĄnica; Argentina.Fil: Strumia, Miriam Cristina. Universidad Nacional de CĂłrdoba. Facultad de Ciencias QuĂmicas. Departamento de QuĂmica OrgĂĄnica; Argentina.Fil: Gomez, Cesar Gerardo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.Fil: Gomez, Cesar Gerardo. Universidad Nacional de CĂłrdoba. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.Fil: Strumia, Miriam Cristina. Universidad Nacional de CĂłrdoba. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.Polymer matrices with well defined structure and pore sizes are widely used in several areas of chemistry such as catalysis, enzyme immobilization, HPLC, adsorbents or controlled drug release. These polymers have pores in its structure both in the dry and swollen state. Although it is well known that the structures and properties greatly differ between these two states, only few methods provide information about the swollen one, even though most of the applications involve the matrices in this situation. Nuclear Magnetic Resonance (NMR) is a suitable tool for the study of the molecular dynamics of different liquids spatially confined in macro, meso and nanopores, through changes in the relaxation times. In transverse relaxation experiments, either diffusion inside the pore, or relaxation induced by mobility restriction of the liquid near the wall, are additional
sources of relaxation, which are extremely useful in the determination of structural and functional properties.Fil: Velasco, Manuel Isaac. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Silletta, Emilia Victoria. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Monti, Gustavo Alberto. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Acosta, Rodolfo HĂ©ctor. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de FĂsica Enrique Gaviola; Argentina.Fil: Velasco, Manuel Isaac. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Silletta, Emilia Victoria. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Monti, Gustavo Alberto. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Acosta, Rodolfo HĂ©ctor. Universidad Nacional de CĂłrdoba. Facultad de MatemĂĄtica, AstronomĂa y FĂsica; Argentina.Fil: Gomez, Cesar Gerardo. Universidad Nacional de CĂłrdoba. Facultad de Ciencias QuĂmicas. Departamento de QuĂmica OrgĂĄnica; Argentina.Fil: Strumia, Miriam Cristina. Universidad Nacional de CĂłrdoba. Facultad de Ciencias QuĂmicas. Departamento de QuĂmica OrgĂĄnica; Argentina.Fil: Gomez, Cesar Gerardo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.Fil: Gomez, Cesar Gerardo. Universidad Nacional de CĂłrdoba. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.Fil: Strumia, Miriam Cristina. Universidad Nacional de CĂłrdoba. Instituto Multidisciplinario de BiologĂa Vegetal; Argentina.FĂsica de los Materiales Condensado
Living Bacterial Sacrificial Porogens to Engineer Decellularized Porous Scaffolds
Decellularization and cellularization of organs have emerged as disruptive methods in tissue engineering and regenerative medicine. Porous hydrogel scaffolds have widespread applications in tissue engineering, regenerative medicine and drug discovery as viable tissue mimics. However, the existing hydrogel fabrication techniques suffer from limited control over pore interconnectivity, density and size, which leads to inefficient nutrient and oxygen transport to cells embedded in the scaffolds. Here, we demonstrated an innovative approach to develop a new platform for tissue engineered constructs using live bacteria as sacrificial porogens. E.coli were patterned and cultured in an interconnected three-dimensional (3D) hydrogel network. The growing bacteria created interconnected micropores and microchannels. Then, the scafold was decellularized, and bacteria were eliminated from the scaffold through lysing and washing steps. This 3D porous network method combined with bioprinting has the potential to be broadly applicable and compatible with tissue specific applications allowing seeding of stem cells and other cell types
Robust open cellular porous polymer monoliths made from cured colloidal gels of latex particles
The coagulation of oppositely charged latexes, prepared from the soap-free emulsion polymerisation of styrene using water as the reaction medium, resulted in the obtainment of colloidal gels that were porous in nature and held together by electrostatic interactions. Chemical crosslinking, involving the introduction of a water-soluble crosslinker, resulted in the obtainment of stronger chemical bonds between particles affording a rigid porous material known as a monolith. It was found that, in a simpler approach, these materials could be prepared using a single latex where the addition of ammonium persulfate both resulted in the formation of the colloidal gel and initiated the crosslinking process. The pore size of the resulting monoliths was predictable as this was observed to directly correlate to the particle diameter, with larger pores achieved using particles of increased size. All gels obtained in this work were highly mouldable and retained their shape, which allowed for a range of formats to be easily prepared without the requirement of a mould
Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent
A continuous supermacroporous matrix has been developed allowing direct capture of enzyme from non-clarified crude cell homogenate at high flow-rates. The continuous supermacroporous matrix has been produced by radical co-polymerization of acrylamide, allyl glycidyl ether and N,NâČ-methylene-bis(acrylamide) which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the column contains a continuous matrix having interconnected pores of 10â100 m size. Iminodiacetic acid covalently coupled to the cryogel is a rendering possibility for immobilized metal affinity chromatographic purification of recombinant His-tagged lactate dehydrogenase, (His)6-LDH, originating from thermophilic bacterium Bacillus stearothermophilus, but expressed in Escherichia coli. The large pore size of the adsorbent makes it possible to process particulate-containing material without blocking the column. No preliminary filtration or centrifugation is needed before application of crude extract on the supermacroporous column. A total of 210 ml crude homogenate, 75 ml of it non-clarified, was processed on a single 5.0 ml supermacroporous column at flow speeds up to 12.5 ml/min without noticeable impairment of the column properties. Mechanically the cryogel adsorbent is very stable. The continuous matrix could easily be removed from the column, dried at 70 °C and kept in a dry state. After rehydration and reinsertion of the matrix into an empty column, (His)6-LDH was purified as efficiently as on the newly prepared column. The procedure of manufacturing the supermacroporous continuous cryogel is technically simple. Starting materials and initiators are cheap and available and are simply mixed and frozen under specified conditions. Altogether these qualities reveal that the supermacroporous continuous cryogels is a very interesting alternative to existing methods of protein purification from particulate-containing crude extract