Full Characterization of the First 1 Inch Industrial Prototype of a New Concept Photodetector

The VSiPMT (Vacuum Silicon PhotoMultiplier Tube) is an original design for an innovative light detector we proposed with the aim to create new scientific instrumentation for industrial applications and physics research. The idea behind this device is to replace the classical dynode chain of a photomultiplier tube with a silicon photomultiplier, the latter acting as an electron detector and amplifier. The VSiPMT offers very attractive features and unprecedented performance, definitely superior to every other photodetector with comparable sensitive surface, such as: negligible power cosumption, excellent photon counting, easy low-voltage-based stabilization and very good time performance. After the feasibility test of the idea, Hamamatsu Photonics realized for our research group two VSiPMT industrial prototypes, that have been fully characterized. The results of the full characterization of the 1-inch industrial prototype are presented in this work.Comment: 11 pages, 14 figure

CHOOSING BY ADVANTAGES IN PROJECT TEAM DECISIONS: CASE STUDIES OF CONCRETE WORKS IN SOUTH AFRICA TO ICIDA 2016

Published Conference ProceedingsGood decision-making is a fundamental element of improved project delivery in the construction industry. Such decisions determine the accomplishment of performance parameters in a given project. However, suboptimal performance, which is pervasive in the construction industry, has been linked to team decisions that undermine project actions. This problem exists in South African construction. To remedy decision-making pitfalls, the concept of Choosing by Advantages (CBA) is now promoted by lean construction. CBA is defined as the decision-making system, which must be based on the importance of advantages within a project team. The purpose of the proposed study is to explore the application of CBA in South Africa. The case-based method to be used for data collection is intended to reveal the characteristics of CBA system so that the advantages could be used to promote its application in South Africa. The impact of CBA on decisions and actions at the activity level shall be examined for improved project performance in South Africa. CBA method is preferred because it encourages decision, which focuses on the advantages of the project rather than the disadvantages of the project. These methods can be adopted when working either on large or small project

Tectonic control on the petrophysical properties of foredeep sandstone in the Central Apennines, Italy

Petrophysical properties of rocks and their applicability at larger scale are a challenging topic in Earth sciences. Petrophysical properties of rocks are severely affected by boundary conditions, rock fabric/microstructure, and tectonics that require a multiscale approach to be properly defined. Here we (1) report laboratory measurements of density, porosity, permeability, and P wave velocities at increasing confining pressure conducted on Miocene foredeep sandstones (Frosinone Formation); (2) compare the laboratory results with larger-scale geophysical investigations; and (3) discuss the effect of thrusting on the properties of sandstones. At ambient pressure, laboratory porosity varied from 2.2% to 13.8% and P wave velocities (Vp) from 1.5 km/s to 2.7 km/s. The P wave velocity increased with confining pressure, reaching between 3.3 km/s and 4.7 km/s at 100 MPa. In situ Vp profiles, measured using sonic logs, matched the ultrasonic laboratory measurement well. The permeability varied between 1.4 × 10 15m2 and 3.9 × 10 15m2 and was positively correlated with porosity. The porosity and permeability of samples taken at various distances to the Olevano–Antrodoco fault plane progressively decreased with distance while P wave velocity increased. At about 1 km from the fault plane, the relative variations reached 43%, 65%, and 20% for porosity, permeability, and P wave velocity, respectively. This suggests that tectonic loading changed the petrophysical properties inherited from sedimentation and diagenesis. Using field constraints and assuming overburden-related inelastic compaction in the proximity of the fault plane, we conclude that the fault reached the mechanical condition for rupture in compression at differential stress of 64.8 MPa at a depth of 1500 m

Probing the Ultrastructure of Spheroids and Their Uptake of Magnetic Nanoparticles by FIB–SEM

Spheroids are 3D cellular systems largely adopted as model for high-throughput screening of molecules and diagnostics tools. Furthermore, those cellular platforms also represent a model for testing new delivery carries for selective targeting. The coupling between the 3D cell environment and the nanovectors can be explored at the macroscale by optical microscopy. However, the nanomaterial-cell interplay finds major action at the single cell and extracellular matrix level with nanoscale interactions. Electron microscopy offers the resolution to investigate those interactions; however, the specimen preparation finds major drawbacks in its operation time and preciseness. In this context, focused ion beam and scanning electron microscopy (FIB–SEM) allows for fast processing and high resolution of the cell-nanomaterial interface. Here, in fact, a novel approach is shown to prepare large-area 3D spheroid cell culture specimens for FIB–SEM. Sectioning procedures are explored to preserve the peculiar structure of spheroids and their interaction with magnetic nanovectors. The results pave the way for advanced investigations of 3D cellular systems with nano and micromaterials relevant to tissue engineering, bioelectronics, and diagnostics

A new generation photodetector for astroparticle physics: the VSiPMT

The VSiPMT (Vacuum Silicon PhotoMultiplier Tube) is an innovative design we proposed for a revolutionary photon detector. The main idea is to replace the classical dynode chain of a PMT with a SiPM (G-APD), the latter acting as an electron detector and amplifier. The aim is to match the large sensitive area of a photocathode with the performance of the SiPM technology. The VSiPMT has many attractive features. In particular, a low power consumption and an excellent photon counting capability. To prove the feasibility of the idea we first tested the performance of a special non-windowed SiPM by Hamamatsu (MPPC) as electron detector and current amplifier. Thanks to this result Hamamatsu realized two VSiPMT industrial prototypes. In this work, we present the results of a full characterization of the VSiPMT prototype

PTEN as a predictive marker of response to conservative treatment in endometrial hyperplasia and early endometrial cancer. A systematic review and meta-analysis

OBJECTIVE: Several markers have been studied to predict the responsiveness of endometrial hyperplasia (EH) and early endometrial cancer (EEC) to progestin therapy. PTEN has played a major role in this field, although its predictive significance is still undefined. We aimed to assess if loss of PTEN expression on pre-treatment endometrial specimen may be a predictive markers of response to progestins in EH and EEC. STUDY DESIGN: MEDLINE, EMBASE, Web of Sciences, Scopus, ClinicalTrial.gov, OVID and Cochrane Library were searched for relevant articles from the inception to May 2018. All studies assessing PTEN expression as predictive marker in EH and EEC treated with progestin were included. Relative risk (RR) for therapy failure was calculated with 95% confidence interval (CI) and a significant p-value<0.05, with a subgroup analysis based on the histologic category (EEC or EH) and the administration route of progestin (oral or intrauterine). RESULTS: Seven cohort studies assessing 376 patients were included. PTEN loss was not significantly associated with the outcome of therapy in the overall analysis (RR = 1.24, 95% CI, 0.88-1.76, p = 0.21), in + the subgroups of EEC (RR = 0.89, 0.32-2.49, p = 0.83), EH (RR = 1.30, 0.90-1.87 p = 0.16), oral progestin (RR = 1.25 0.88-1.79, p = 0.22) and intrauterine device (RR = 1.02, 0.36-2.87, p = 0.97). CONCLUSION: PTEN seems not to be useful as predictive marker of response to the conservative treatment of EH and EC, regardless of the administration route (oral or intrauterine) of progestins. We advise future researcher not to further assess PTEN as a stand-alone predictive marker

‘Pole Test’ Measurements in Critical Leg Ischaemia

AbstractBackgroundFor the quantification of critical limb ischaemia (CLI) most vascular surgery units use sphygmo-manometric and transcutaneous oxygen pressure (TcPO2) measurements. However, measurements obtained by cuff-manometry can be overestimated especially in diabetic patients because of medial calcification that makes leg arteries less compressible. TcPO2 measurements present a considerable overlap in the values obtained for patients with different degrees of ischaemia and its reproducibility has been questioned. Arterial wall stiffness has less influence on the pole test, based on hydrostatic pressure derived by leg elevation, and this test seems to provide a reliable index of CLI.ObjectiveThe objective of this study was to evaluate the pole pressure test for detection of critical lower limb ischaemia, correlating results with cuff-manometry and transcutaneous oxygen pressure.DesignUniversity hospital-prospective study.Materials and methodsSeventy-four patients (83 legs) with rest pain or gangrene were evaluated by four methods: pole test, cuff-manometry, TcPO2 and arteriography. CLI was present if the following criteria were met: (a) important arteriographic lesions+rest pain with an ankle systolic pressure (ASP) ≤40mmHg and/or a TcPO2 ≤30mmHg, or (b) important arteriographic lesions+tissue loss with an ASP ≤60mmHg and/or a TcPO2 ≤40mmHg. Fifty-seven lower limbs met the criteria for CLI.ResultsMeasurements obtained by cuff-manometry were significantly higher to those obtained by pole test (mean pressure difference: 40mmHg, p<0.001). The difference between the two methods remained statistically significant for both diabetics (50.73, p<0.001) and non-diabetics (31.46, p<0.001). Mean TcPO2 value was 15.51mmHg and there was no important difference between patients with and without diabetes. Overall, there was a correlation between sphygmomanometry and pole test (r=0.481). The correlation persisted for patients without diabetes (r=0.581), but was not evident in patients with diabetes. Correlation between pole test and TcPO2 was observed only for patients with diabetes (r=0.444). There was no correlation between cuff-manometry and TcPO2. The pole test offered an accuracy of 88% for the detection of CLI. The sensitivity of this test was 95% and the specificity 73%

Electron Microscopy for 3D Scaffolds–Cell Biointerface Characterization

Cell fate is largely determined by interactions that occur at the interface between cells and their surrounding microenvironment. For this reason, especially in the field of tissue-engineering, there is a growing interest in developing techniques that allow evaluating cell–material interaction at the nanoscale, particularly focusing on cell adhesion processes. While for 2D culturing systems a consolidated series of tools already satisfy this need, in 3D environments, more closely recapitulating complex in vivo structures, there is still a lack of procedures furthering the comprehension of cell–material interactions. Here, the use of scanning electron microscopy coupled with a focused ion beam (SEM/FIB) for the characterization of cell interactions with 3D scaffolds obtained by different fabrication techniques is reported for the first time. The results clearly show the capability of the developed approach to preserve and finely resolve scaffold–cell interfaces highlighting details such as plasma membrane arrangement, extracellular matrix architecture and composition, and cellular structures playing a role in cell adhesion to the surface. It is anticipated that the developed approach will be relevant for the design of efficient cell-instructive platforms in the study of cellular guidance strategies for tissue-engineering applications as well as for in vitro 3D models

Fault structure and slip localization in carbonate-bearing normal faults: An example from the Northern Apennines of Italy

Carbonate-bearing normal faults are important structures for controlling fluid flow and seismogenesis within the brittle upper crust. Numerous studies have tried to characterize fault zone structure and earthquake slip processes along carbonate-bearing faults. However, due to the different scales of investigation, these studies are not often integrated to provide a comprehensive fault image. Here we present a multi-scale investigation of a normal fault exhumed from seismogenic depths. The fault extends for a length of 10 km with a maximum width of about 1.5 km and consists of 5 sub-parallel and interacting segments. The maximum displacement (370e650 m) of each fault segment is partitioned along sub-parallel slipping zones extending for a total width of about 50 m. Each slipping zone is characterized by slipping surfaces exhibiting different slip plane phenomena. Fault rock development is controlled by the protolith lithology. In massive limestone, moving away from the slip surface, we observe a thin layer (<2 cm) of ultracataclasite, cataclasite (2e10 cm) and fault breccia. In marly limestone, the fault rock consists of a cataclasite with hydrofractures and smectite-rich pressure solution seams. At the micro-nanoscale, the slip surface consists of a continuous and thin (<300 mm) layer composed of coarse calcite grains (~5e20 mm in size) associated with sub-micrometer grains showing fading grain boundaries, voids and/or vesicles, and suggesting thermal decomposition processes. Micrometer-sized calcite crystals show nanoscale polysynthetic twinning affected by the occurrence of subgrain boundaries and polygonalized nanostructures. Investigations at the kilometres-tens of meter scale provide fault images that can be directly compared with high-resolution seismological data and when combined can be used to develop a comprehensive characterization of seismically active fault structures in carbonate lithologies. Micro and nanoscale investigations along the principal slipping zone suggest that different deformation processes, including plastic deformation and thermal decomposition, were active during seismic slip

Study of the stability of a paramagnetic label linked to mesoporous silica surface in contact with rat mesothelial cells in culture.

Stable radicals detectable by electron paramagnetic resonance (EPR) may be use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served as probes in the investigation of the effects of incubation of silica particles with mesothelial cells. A mesoporous silica (MCM-41), prepared by precipitation from a micellar solution, was the most appropriate silica-based particle for this purpose, as its channels allow direct contact with small molecules but not with macromolecules. The cytotoxicity of this amorphous silica is very low, allowing relatively high particle loading in the cell cultures. Both the high surface area of the sample and the large amount of inorganic material extracted from the cell culture provide enough material to run reasonably intense EPR spectra. Computer-aided analysis of the EPR spectra of silica-bound nitroxides provided information on the sensitivity of the labeled silica monitoring different environments, e.g., to follow the path of particles in a mammalian cell culture. Upon contact of the particles with mesothelial cells, the mean distance among the labels at the silica surface decreased as a consequence of the release of oxidizing and/or radical moieties from the cells