Multianalyte Sensing Of Addictive Over-the-counter (otc) Drugs

A supramolecular sensor array composed of two fluorescent cucurbit[n]uril-type receptors (probe 1 and probe 2) displaying complementary selectivities was tested for its ability to detect and quantify drug-related amines. The fluorimetric titration of the individual probes showed highly variable and cross-reactive analyte-dependent changes in fluorescence. An excellent ability to recognize a variety of analytes was demonstrated in qualitative as well as quantitative assays. Importantly, a successful quantitative analysis of several analytes of interest was achieved in mixtures and in human urine. The throughput and sensitivity surpass those of the current state-of-the-art methods that usually require analyte solid-phase extraction (SPE). These results open up the opportunity for new applications of cucurbit[n]uril-type receptors in sensing and pave the way for the development of simple high-throughput assays for various drugs in the near future

Modelling the covariance structure in marginal multivariate count models: Hunting in Bioko Island.

The main goal of this article is to present a flexible statistical modelling framework to deal with multivariate count data along with longitudinal and repeated measures structures. The covariance structure for each response variable is defined in terms of a covariance link function combined with a matrix linear predictor involving known matrices. In order to specify the joint covariance matrix for the multivariate response vector, the generalized Kronecker product is employed. We take into account the count nature of the data by means of the power dispersion function associated with the Poisson–Tweedie distribution. Furthermore, the score information criterion is extended for selecting the components of the matrix linear predictor. We analyse a data set consisting of prey animals (the main hunted species, the blue duiker Philantomba monticola and other taxa) shot or snared for bushmeat by 52 commercial hunters over a 33-month period in Pico Basilé, Bioko Island, Equatorial Guinea. By taking into account the severely unbalanced repeated measures and longitudinal structures induced by the hunters and a set of potential covariates (which in turn affect the mean and covariance structures), our method can be used to indicate whether there was statistical evidence of a decline in blue duikers and other species hunted during the study period. Determining whether observed drops in the number of animals hunted are indeed true is crucial to assess whether species depletion effects are taking place in exploited areas anywhere in the world. We suggest that our method can be used to more accurately understand the trajectories of animals hunted for commercial or subsistence purposes and establish clear policies to ensure sustainable hunting practices

Spatial Autoregressive Models for Circular Data

A class of autoregressive models for spatial circular data is proposed by assuming that samples of angular measurements are drawn from a multivariate von Mises distribution with mean and concentration parameters that depend on covariates through link functions. The model can flexibly accommodate heteroscedasticity and specific autoregressive correlation structures. Because the computation of the normalizing constant of the multivariate von Mises distribution is unfeasible, inference is based on a computationally tractable Monte Carlo approximation of the log-likelihood. These methods are illustrated on a case study of marine currents in the Northern Adriatic sea

A Copula-Based Hidden Markov Model for Toroidal Time Series

Toroidal time series are temporal sequences of bivariate angular observations that often arise in environmental and ecological studies. A hidden Markov model is proposed for segmenting these data according to a finite number of latent classes, associated with copula-based toroidal densities. The model conveniently integrates circular correlation, multimodality and temporal auto-correlation. A computationally efficient EM algorithm is proposed for parameter estimation. The proposal is illustrated on a time series of wind and sea wave directions