An in vitro DNA sensor-based assay to measure receptor-specific adhesion forces of eukaryotic cells and pathogens

Motility of eukaryotic cells or pathogens within tissues is mediated by the turnover of specific interactions with other cells or with the extracellular matrix. Biophysical characterization of these ligand-receptor adhesions helps to unravel the molecular mechanisms driving migration. Traction force microscopy or optical tweezers are typically used to measure the cellular forces exerted by cells on a substrate. However, the spatial resolution of traction force microscopy is limited to ~2 μm and performing experiments with optical traps is very time-consuming. Here we present the production of biomimetic surfaces that enable specific cell adhesion via synthetic ligands and at the same time monitor the transmitted forces by using molecular tension sensors. The ligands were coupled to double-stranded DNA probes with defined force thresholds for DNA unzipping. Receptor-mediated forces in the pN range are thereby semi-quantitatively converted into fluorescence signals, which can be detected by standard fluorescence microscopy at the resolution limit (~0.2 μm). The modular design of the assay allows to vary the presented ligands and the mechanical strength of the DNA probes, which provides a number of possibilities to probe the adhesion of different eukaryotic cell types and pathogens and is exemplified here with osteosarcoma cells and Plasmodium berghei Sporozoites

Boron depletion in 9 to 15 M(circle dot) stars with rotation

The treatment of mixing is still one of the major uncertainties in stellar evolution models. One open question is how well the prescriptions for rotational mixing describe the real effects. We tested the mixing prescriptions included in the Geneva stellar evolution code (GENEC) by following the evolution of surface abundances of light isotopes in massive stars, such as boron and nitrogen. We followed 9, 12 and 15 M(O) models with rotation from the zero age main sequence up to the end of He burning. The calculations show the expected behaviour with faster depletion of boton for faster rotating stars and more massive stars. The mixing at the surface is more efficient, than predicted by prescriptions used in other codes and reproduces the majority of observations very well However two observed stars with strong boron depletion but, no nitrogen enhancement still can not be explained and let the question open whether additional mixing processes are acting in these massive star

Phase separation in a chaotic flow

The phase separation between two immiscible liquids advected by a bidimensional velocity field is investigated numerically by solving the corresponding Cahn-Hilliard equation. We study how the spinodal decomposition process depends on the presence -or absence- of Lagrangian chaos. A fully chaotic flow, in particular, limits the growth of domains and for unequal volume fractions of the liquids, a characteristic exponential distribution of droplet sizes is obtained. The limiting domain size results from a balance between chaotic mixing and spinodal decomposition, measured in terms of Lyapunov exponent and diffusivity constant, respectively.Comment: Minor changes - Version accepted for publication - Physical Review Letter

Umbilical endometriosis

We report two women who presented with a recurrent, mildly painful, bluish nodule in the umbilicus. Both patients complained of local tenderness and occasional bleeding that increased during menstruation. Neither patient had had previous pelvic surgery. Excision of the lesions revealed a primary umbilical endometriosis; in one case, a simultaneous laparoscopy showed a pelvic endometriosis. We review the current literature and discuss the possible etiopathogenesis and when a laparoscopy is indicated to diagnose a concomitant pelvic endometriosis. Umbilical endometriosis is a very rare disease but should be considered in the differential diagnosis of umbilical lesion

Case report of long-term postural tachycardia syndrome in a patient after messenger RNA coronavirus disease-19 vaccination with mRNA-1273

BACKGROUND Postural tachycardia syndrome (POTS) is characterized by orthostatic intolerance and heart rate increase in an upright position without orthostatic hypotension. It has been described after coronavirus disease-19 (COVID-19) as well as after COVID-19 vaccination. CASE SUMMARY A 54-year-old female patient presented with a 9-months history of severe orthostatic intolerance since COVID-19 vaccination with messenger RNA (mRNA)-1273 (Spikevax, Moderna). Except for diet-controlled coeliac disease, the patient was healthy, had no allergies, and did not take regular medication. Tilt table testing revealed a significant heart rate increase to 168 bpm without orthostatic hypotension accompanied by light-headedness, nausea, and syncope, findings consistent with POTS. Potential underlying causes including anaemia, thyroid dysfunction, adrenal insufficiency, pheochromocytoma, (auto)-immune disease, chronic inflammation as well as neurological causes were ruled out. Echocardiography and cardiac stress magnetic resonance imaging (MRI) did not detect structural or functional heart disease or myocardial ischaemia. Forty-eight-hour-electrocardiogram (ECG) showed no tachycardias other than sinus tachycardia. Finally, genomic analysis did not detect an inherited arrhythmia syndrome. Serologic analysis revealed adequate immune response to mRNA-1273 vaccination without signs of previous severe acute respiratory syndrome-coronavirus-2 infection. While ivabradine was not tolerated and metoprolol extended release only slightly improved symptoms, physical exercise reduced orthostatic intolerance moderately. At a 5-months follow-up, the patient remained dependant on assistance for activities of daily living. DISCUSSION The temporal association of POTS with the COVID-19 vaccination in a previously healthy patient and the lack of evidence of an alternative aetiology suggests COVID-19 vaccination is the potential cause of POTS in this patient. To our knowledge, this is the first case reporting severe, long-term, and treatment-refractory POTS following COVID-19 vaccination with mRNA1273

Stellar Evolution in the Early Universe

Massive stars played a key role in the early evolution of the Universe. They formed with the first halos and started the re-ionisation. It is therefore very important to understand their evolution. In this paper, we describe the strong impact of rotation induced mixing and mass loss at very low $Z$. The strong mixing leads to a significant production of primary nitrogen 14, carbon 13 and neon 22. Mass loss during the red supergiant stage allows the production of Wolf-Rayet stars, type Ib,c supernovae and possibly gamma-ray bursts (GRBs) down to almost Z=0 for stars more massive than 60 solar masses. Galactic chemical evolution models calculated with models of rotating stars better reproduce the early evolution of N/O, C/O and C12/C13. We calculated the weak s-process production induced by the primary neon 22 and obtain overproduction factors (relative to the initial composition, Z=1.e-6) between 100-1000 in the mass range 60-90.Comment: 8 pages, 4 figures, proceedings of IAU Symposium 255, "Low-Metallicity Star Formation: From the First stars to Dwarf Galaxies", L.K. Hunt, S. Madden & R. Schneider, ed

Boron depletion in 9 to 15 M stars with rotation

The treatment of mixing is still one of the major uncertainties in stellar evolution models. One open question is how well the prescriptions for rotational mixing describe the real effects. We tested the mixing prescriptions included in the Geneva stellar evolution code (GENEC) by following the evolution of surface abundances of light isotopes in massive stars, such as boron and nitrogen. We followed 9, 12 and 15 M models with rotation from the zero age main sequence up to the end of He burning. The calculations show the expected behaviour with faster depletion of boron for faster rotating stars and more massive stars. The mixing at the surface is more efficient than predicted by prescriptions used in other codes and reproduces the majority of observations very well. However two observed stars with strong boron depletion but no nitrogen enrichment still can not be explained and let the question open whether additional mixing processes are acting in these massive star

Stellar Evolution in the Early Universe

Massive stars played a key role in the early evolution of the Universe. They formed with the first halos and started the re-ionisation. It is therefore very important to understand their evolution. In this paper, we describe the strong impact of rotation induced mixing and mass loss at very low metallicity (Z). The strong mixing leads to a significant production of primary 14N, 13C and 22Ne. Mass loss during the red supergiant stage allows the production of Wolf-Rayet stars, type Ib,c supernovae and possibly gamma-ray bursts (GRBs) down to almost Z = 0 for stars more massive than 60 M. Galactic chemical evolution models calculated with models of rotating stars better reproduce the early evolution of N/O, C/O and 12C/13C. We calculated the weak s-process production induced by the primary 22Ne and obtain overproduction factors (relative to the initial composition, Z = 10−6) between 100-1000 in the mass range 60-90

Embodied GHG emissions of buildings – The hidden challenge for effective climate change mitigation

Buildings are major sources of greenhouse gas (GHG) emissions and contributors to the climate crisis. To meet climate-change mitigation needs, one must go beyond operational energy consumption and related GHG emissions of buildings and address their full life cycle. This study investigates the global trends of GHG emissions arising across the life cycle of buildings by systematically compiling and analysing more than 650 life cycle assessment (LCA) case studies. The results, presented for different energy performance classes based on a final sample of 238 cases, show a clear reduction trend in life cycle GHG emissions due to improved operational energy performance. However, the analysis reveals an increase in relative and absolute contributions of so‐called ‘embodied’ GHG emissions, i.e., emissions arising from manufacturing and processing of building materials. While the average share of embodied GHG emissions from buildings following current energy performance regulations is approximately 20–25% of life cycle GHG emissions, this figure escalates to 45–50% for highly energy-efficient buildings and surpasses 90% in extreme cases. Furthermore, this study analyses GHG emissions at time of occurrence, highlighting the ‘carbon spike’ from building production. Relating the results to existing benchmarks for buildings’ GHG emissions in the Swiss SIA energy efficiency path shows that most cases exceed the target of 11.0 kgCO$^{2}$eq/m$^{2}$a. Considering global GHG reduction targets, these results emphasize the urgent need to reduce GHG emissions of buildings by optimizing both operational and embodied impacts. The analysis further confirmed a need for improving transparency and comparability of LCA studies