F2 region maximum electron density height predictions for South American latitudes

Values of the F2 region maximum electron density height (hmF2) calculated using ground ionosonde data at South American latitudes are used to check the validity of the International Reference Ionosphere (IRI) to predict this variable. With this in mind we compare hmF2 predictions given by the model when measurements of critical frequency of F2 region and propagation parameter M(3000)F2 were used as input parameter in IRI (hmF2IRI-Exp), against those obtained using the standard International Radio Consultative Committee (CCIR) option (hmF2IRI-CCIR)- In this paper we used hmF2 IRI-Exp values because hmF2 measurements were not available for the considered cases. Moreover, a comparison of the measured M(3000)F2 values with the CCIR predictions have been done. The results show that, in general, the standard predictions follow the diurnal tendency observed in the hmF2 IRI-Exp values. At low latitudes the hmF2IRI-Exp values show oscillations not reproduced by the standard option. Cases with disagreements for 24 hours have been observed at high latitudes. Other cases with good agreement have been also obtained. The results suggest that, in general, the standard option of the model gives good hmF2 predictions at South American latitudes. Few cases showed deviation between 15 and 25%. As we expected, the obtained results suggest that the deviation between predicted and measured M(3000)F2 values is the main contribution for the deviation between hmF2IRI-CCIR and hmF2IRI-Exp. The comparison with the results obtained in previous work shows that the IRI performance in predicting M(3000)F2 and hmF2 is better than in predicting foF2.Fil: Ezquer, Rodolfo Gerardo. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Física. Laboratorio de Ionósfera; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Scidá, Luis. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Física. Laboratorio de Ionósfera; ArgentinaFil: Mansilla, Gustavo Adolfo. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Física. Laboratorio de Ionósfera; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Mosert, Marta Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; ArgentinaFil: Herrera, Marcelo F.. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Física. Laboratorio de Ionósfera; Argentin

Propagation of gravity waves and spread F in the low-latitude ionosphere over Tucumán, Argentina, by continuous Doppler sounding: first results

Results of systematic analysis of propagation directions and horizontal velocities of gravity waves (GWs) and spread F structures in low-latitude ionosphere (magnetic inclination ~27°) in Tucumán region, Argentina, are presented. Measurements were carried out by multipoint continuous Doppler system during 1 year from December 2012 to November 2013. It was found that meridian propagation of GWs dominated and that southward propagation prevailed in the local summer. Oblique spread structures observed in Doppler shift spectrograms and associated with spread F propagated roughly eastward at velocities from ~70 to ~180 m/s and were observed at night from ~ September to ~ March. The velocities were computed for 182 events and the azimuths for 64 events. Continuous Doppler sounding makes it possible to analyze more events compared to optical observations often used for propagation studies since the measurements do not depend on weather.Fil: Chum, J.. Institute of Atmospheric Physics; República ChecaFil: Miranda Bonomi, Fernando Alberto. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Electricidad, Electrónica y Computación. Laboratorio de Telecomunicaciones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fišer, J.. Institute of Atmospheric Physics; República ChecaFil: Cabrera, M. A.. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Electricidad, Electrónica y Computación. Laboratorio de Telecomunicaciones; Argentina. Universidad Tecnológica Nacional. Facultad Regional Tucuman; ArgentinaFil: Ezquer, Rodolfo Gerardo. Universidad Tecnológica Nacional. Facultad Regional Tucuman; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Física. Laboratorio de Ionosfera; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Burešová, D.. Institute of Atmospheric Physics; República ChecaFil: Laštovička, J.. Institute of Atmospheric Physics; República ChecaFil: Baše, J.. Institute of Atmospheric Physics; República ChecaFil: Hruška, F.. Institute of Atmospheric Physics; República ChecaFil: Molina, Maria Graciela. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Ciencias de la Computación; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ise, Juan Eduardo. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Electricidad, Electrónica y Computación. Laboratorio de Telecomunicaciones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cangemi, José Ignacio. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología. Departamento de Electricidad, Electrónica y Computación. Laboratorio de Telecomunicaciones; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Šindelářová, T.. Institute of Atmospheric Physics; República Chec

Mannose receptor may be involved in small ruminant lentivirus pathogenesis

Thirty-one sheep naturally infected with small ruminant lentiviruses (SRLV) of known genotype (A or B), and clinically affected with neurological disease, pneumonia or arthritis were used to analyse mannose receptor (MR) expression (transcript levels) and proviral load in virus target tissues (lung, mammary gland, CNS and carpal joints). Control sheep were SRLV-seropositive asymptomatic (n = 3), seronegative (n = 3) or with chronic listeriosis, pseudotuberculosis or parasitic cysts (n = 1 in each case). MR expression and proviral load increased with the severity of lesions in most analyzed organs of the SRLV infected sheep and was detected in the affected tissue involved in the corresponding clinical disease (CNS, lung and carpal joint in neurological disease, pneumonia and arthritis animal groups, respectively). The increased MR expression appeared to be SRLV specific and may have a role in lentiviral pathogenesis.Funded by grants from CICYT AGL2010-22341-C04-01 and Gobierno de Navarra IIQ14064.RI1. We acknowledge the Public University of Navarra and CSIC for fellowships and the JAE-contract (HC and RR)

The Antidiabetic Effect of MSCs Is Not Impaired by Insulin Prophylaxis and Is Not Improved by a Second Dose of Cells

Type 1 diabetes mellitus (T1D) is due to autoimmune destruction of pancreatic beta-cells. Previously, we have shown that intravenously administered bone marrow-derived multipotent mesenchymal stromal cells (MSCs) allows pancreatic islet recovery, improves insulin secretion and reverts hyperglycemia in low doses streptozotocin (STZ)-induced diabetic mice. Here we evaluate whether insulin prophylaxis and the administration of a second dose of cells affect the antidiabetic therapeutic effect of MSC transplantation. Insulitis and subsequent elimination of pancreatic beta-cells was promoted in C57BL/6 mice by the injection of 40 mg/kg/day STZ for five days. Twenty-four days later, diabetic mice were distributed into experimental groups according to if they received or not insulin and/or one or two doses of healthy donor-derived MSCs. Three and half months later: glycemia, pancreatic islets number, insulinemia, glycated hemoglobin level and glucose tolerance were determined in animals that did not received exogenous insulin for the last 1.5 months. Also, we characterized MSCs isolated from mice healthy or diabetic. The therapeutic effect of MSC transplantation was observed in diabetic mice that received or not insulin prophylaxis. Improvements were similar irrespective if they received one or two doses of cells. Compared to MSCs from healthy mice, MSCs from diabetic mice had the same proliferation and adipogenic potentials, but were less abundant, with altered immunophenotype and no osteogenic potential

Exosomes released upon mitochondrial ASncmtRNA knockdown reduce tumorigenic properties of malignant breast cancer cells

Indexación ScopusDuring intercellular communication, cells release extracellular vesicles such as exosomes, which contain proteins, ncRNAs and mRNAs that can influence proliferation and/or trigger apoptosis in recipient cells, and have been proposed to play an essential role in promoting invasion of tumor cells and in the preparation of metastatic niches. Our group proposed the antisense non-coding mitochondrial RNA (ASncmtRNA) as a new target for cancer therapy. ASncmtRNA knockdown using an antisense oligonucleotide (ASO-1537S) causes massive death of tumor cells but not normal cells and strongly reduces metastasis in mice. In this work, we report that exosomes derived from ASO-1537S-treated MDA-MB-231 breast cancer cells (Exo-1537S) inhibits tumorigenesis of recipient cells, in contrast to exosomes derived from control-ASO-treated cells (Exo-C) which, in contrast, enhance these properties. Furthermore, an in vivo murine peritoneal carcinomatosis model showed that Exo-1537S injection reduced tumorigenicity compared to controls. Proteomic analysis revealed the presence of Lactadherin and VE-Cadherin in exosomes derived from untreated cells (Exo-WT) and Exo-C but not in Exo-1537S, and the latter displayed enrichment of proteasomal subunits. These results suggest a role for these proteins in modulation of tumorigenic properties of exosome-recipient cells. Our results shed light on the mechanisms through which ASncmtRNA knockdown affects the preparation of breast cancer metastatic niches in a peritoneal carcinomatosis model. © 2020, The Author(s).https://www-nature-com.recursosbiblioteca.unab.cl/articles/s41598-019-57018-

Observatorio ionosferico Bahía Blanca : Primeros resultados

La ionósfera presenta diferentes comportamientos en baja, media y alta latitud. En Septiembre de 2016, en colaboración con el MINCYT y el Instituto de Geofísica y Vulcanología de Roma (INGV) y con el apoyo de la Universidad Nacional de Tucumán (UNT), la Universidad Tecnológica Nacional (UTN) instaló un moderno ionosonda (Advanced Ionospheric Sounder - AIS) en la Facultad Regional Bahía Blanca (FRBB) (38,7º S, 297,7º E). En este trabajo se presentan los primeros resultados obtenidos con el ionosonda de la FRBB, los que corresponden a Octubre de 2016. Usando medianas y cuartiles de la frecuencia crítica de la región F2 (foF2) se analiza el comportamiento de la ionósfera sobre esta estación de latitud media y, además, se lo compara con el observado en baja latitud, para lo cual se utilizan mediciones simultáneas realizadas con el AIS de Tucumán (26,9º S; 294,6º E). Las mediciones obtenidas con el AIS de la FRBB, también son utilizadas para analizar la confiabilidad del mapa horario de foF2 publicado por el Ionospheric Prediction Service (IPS) de Australia. Los resultados muestran que sobre Bahía Blanca foF2 presenta una variación diaria con un mínimo entre la 1 UT y las 9 UT (antes de las 5 LT) y un máximo a las 17 UT (aprox. 13 LT), para el mes considerado. El estudio comparativo con baja latitud, muestra que los valores de la frecuencia crítica de la región F2 de la ionósfera sobre Tucumán son mayores que los de Bahía Blanca, lo que se debe a la influencia de la Anomalía Ecuatorial sobre la ionósfera de baja latitud. Por otro lado, la variabilidad de foF2 sobre Bahía Blanca mostró ser menor que la correspondiente a la de Tucumán. Además, se muestra un ejemplo de un mapa de foF2 publicado por el IPS con buenos valores para Tucumán pero con una marcada sobreestimación de esta magnitud ionosférica para Bahía Blanca. Esta disparidad de resultados se debe a que el IPS utiliza los datos del AIS de Tucumán para construir el mencionado mapa pero no usa las mediciones del nuevo ionosonda de la FRBB. Se destaca que los datos de la FRBB fueron ofrecidos al IPS para mejorar la confiabilidad del mapa mencionado en la zona Central - Sur de nuestro país.Eje: Acoplamiento Solar-Terrestre en el geoespacio.Facultad de Ciencias Astronómicas y Geofísica

Neonatal mesenchymal stem cell treatment improves myelination impaired by global perinatal asphyxia in rats

Indexación ScopusThe effect of perinatal asphyxia (PA) on oligodendrocyte (OL), neuroinflammation, and cell viability was evaluated in telencephalon of rats at postnatal day (P)1, 7, and 14, a period char-acterized by a spur of neuronal networking, evaluating the effect of mesenchymal stem cell (MSCs)- treatment. The issue was investigated with a rat model of global PA, mimicking a clinical risk oc-curring under labor. PA was induced by immersing fetus-containing uterine horns into a water bath for 21 min (AS), using sibling-caesarean-delivered fetuses (CS) as controls. Two hours after delivery, AS and CS neonates were injected with either 5 μL of vehicle (10% plasma) or 5 × 104 MSCs into the lateral ventricle. Samples were assayed for myelin-basic protein (MBP) levels; Olig-1/Olig-2 tran-scriptional factors; Gglial phenotype; neuroinflammation, and delayed cell death. The main effects were observed at P7, including: (i) A decrease of MBP-immunoreactivity in external capsule, corpus callosum, cingulum, but not in fimbriae of hippocampus; (ii) an increase of Olig-1-mRNA levels; (iii) an increase of IL-6-mRNA, but not in protein levels; (iv) an increase in cell death, including OLs; and (v) MSCs treatment prevented the effect of PA on myelination, OLs number, and cell death. The present findings show that PA induces regional- and developmental-dependent changes on myelination and OLs maturation. Neonatal MSCs treatment improves survival of mature OLs and myelination in telencephalic white matter. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.https://www.mdpi.com/1422-0067/22/6/327

Intranasal administration of mesenchymal stem cell secretome reduces hippocampal oxidative stress, neuroinflammation and cell death, improving the behavioral outcome following perinatal asphyxia

Indexación: Scopus.PerinatalAsphyxia (PA) is a leading cause ofmotor and neuropsychiatric disability associated with sustained oxidative stress, neuroinflammation, and cell death, affecting brain development. Based on a rat model of global PA, we investigated the neuroprotective effect of intranasally administered secretome, derived from human adipose mesenchymal stem cells (MSC-S), preconditioned with either deferoxamine (an hypoxia-mimetic) or TNF-ff+IFN- (pro-inflammatory cytokines). PA was generated by immersing fetus-containing uterine horns in a water bath at 37 ffC for 21 min. Thereafter, 16 ffL of MSC-S (containing 6 ffg of protein derived from 2 ff 105 preconditioned-MSC), or vehicle, were intranasally administered 2 h after birth to asphyxia-exposed and control rats, evaluated at postnatal day (P) 7. Alternatively, pups received a dose of either preconditioned MSC-S or vehicle, both at 2 h and P7, and were evaluated at P14, P30, and P60. The preconditioned MSC-S treatment (i) reversed asphyxia-induced oxidative stress in the hippocampus (oxidized/reduced glutathione); (ii) increased antioxidative Nuclear Erythroid 2-Related Factor 2 (NRF2) translocation; (iii) increased NQO1 antioxidant protein; (iv) reduced neuroinflammation (decreasing nuclearNF-ffB/p65 levels and microglial reactivity); (v) decreased cleaved-caspase-3 cell-death; (vi) improved righting reflex, negative geotaxis, cliff aversion, locomotor activity, anxiety, motor coordination, and recognition memory. Overall, the study demonstrates that intranasal administration of preconditioned MSC-S is a novel therapeutic strategy that prevents the long-term effects of perinatal asphyxia. © 2020 by the authors.https://www.mdpi.com/1422-0067/21/20/780