Evaluation of metabolic profiles of Saanen goats in the transition period

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗHealthy Saanen goats (n=30) in periparturient period were used in the present study. Blood samples were collected 21, 14 and 7 days before parturition, at the time of birth and postpartum days 7, 14 and 21. Non-esterifiedfatty acids (NEFA), betahydroxy butyric acid (BHBA), Total protein (TP), albumin (ALB), blood urea nitrogen (BUN), creatinine (CREA) aspartate amino transferase (AST), gamma glutamyl transferase (GGT), sorbitol dehydrogenase (SDH), glucose (GLU), cholesterol (CHOL), triglyceride (TG), calcium (Ca), phosphorus (P), and magnesium (Mg) levels were evaluated. During the study period, NEFA, SDH, CREA levels increased and CHOL and TG levels decreased at time of parturition. When the changes of parameters in prepartum and postpartum period were compared, the concentrations of NEFA, CHOL, GLU, TG, Ca were higher (p <0.001) in prepartum period;however BHBA, Mg, ALB, GGT, AST, TP, P, BUN, SDH and CREA concentrations were detected to be higher (p <0.001) in postpartum period. Metabolic Profile Test based on biochemical parameters evaluated in our study would be beneficial for diagnosis, prevention and control of diseases such as pregnancy toxemia, hypocalcemia, infertility in goats

Prevalence, symptoms and management of uterine fibroids: an international internet-based survey of 21,746 women

<p>Abstract</p> <p>Background</p> <p>In 2009 the Uterine Bleeding and Pain Women's Research Study (UBP-WRS) was conducted interviewing 21,479 women across 8 countries in order to gain patient-based prevalence data on uterine pain and bleeding indications and investigate uterine symptoms and women's treatment experiences. This article shows relevant results of the study for the indication uterine fibroids providing data on self-reported prevalence, symptomatology and management of uterine fibroids.</p> <p>Methods</p> <p>2,500 women (USA: 4,500 women) in each country (Brazil, Canada, France, Germany, Italy, South Korea, the UK, the USA) completed an online survey. Women included were in their reproductive age (age group 15-49 years; USA: 18-49 years) and had ever experienced menstrual bleedings. Quotas were applied for age, region, level of education and household income of respondents. Variables have been analyzed descriptively and exploratory statistical tests have been performed.</p> <p>Results</p> <p>The self-reported prevalence of uterine fibroids ranged from 4.5% (UK) to 9.8% (Italy), reaching 9.4% (UK) to 17.8% (Italy) in the age group of 40-49 years. Women with a diagnosis of uterine fibroids reported significantly more often about bleeding symptoms than women without a diagnosis: heavy bleedings (59.8% vs. 37.4%), prolonged bleedings (37.3% vs. 15.6%), bleeding between periods (33.3% vs. 13.5%), frequent periods (28.4% vs. 15.2%), irregular and predictable periods (36.3% vs. 23.9%). Furthermore women with diagnosed uterine fibroids reported significantly more often about the following pain symptoms: pressure on the bladder (32.6% vs. 15.0%), chronic pelvic pain (14.5% vs. 2.9%), painful sexual intercourse (23.5% vs. 9.1%) and pain occurring mid-cycle, after and during menstrual bleeding (31.3%, 16.7%, 59.7%, vs. 17.1%, 6.4%, 52.0%). 53.7% of women reported that their symptoms had a negative impact on their life in the last 12 month, influencing their sexual life (42.9%), performance at work (27.7%) and relationship & family (27.2%).</p> <p>Conclusions</p> <p>Uterine fibroid is a common concern in women at fertile age causing multiple bleeding and pain symptoms which can have a negative impact on different aspects in women's life.</p

The Movember Global Action Plan 1 (GAP1): Unique Prostate Cancer Tissue Microarray Resource

BackgroundThe need to better understand the molecular underpinnings of the heterogeneous outcomes of patients with prostate cancer is a pressing global problem and a key research priority for Movember. To address this, the Movember Global Action Plan 1 Unique tissue microarray (GAP1-UTMA) project constructed a set of unique and richly annotated tissue microarrays (TMA) from prostate cancer samples obtained from multiple institutions across several global locations.MethodsThree separate TMA sets were built that differ by purpose and disease state.ResultsThe intended use of TMA1 (Primary Matched LN) is to validate biomarkers that help determine which clinically localized prostate cancers with associated lymph node metastasis have a high risk of progression to lethal castration-resistant metastatic disease, and to compare molecular properties of high-risk index lesions within the prostate to regional lymph node metastases resected at the time of prostatectomy. TMA2 (Pre vs. Post ADT) was designed to address questions regarding risk of castration-resistant prostate cancer (CRPC) and response to suppression of the androgen receptor/androgen axis, and characterization of the castration-resistant phenotype. TMA3 (CRPC Met Heterogeneity)'s intended use is to assess the heterogeneity of molecular markers across different anatomic sites in lethal prostate cancer metastases.ConclusionsThe GAP1-UTMA project has succeeded in combining a large set of tissue specimens from 501 patients with prostate cancer with rich clinical annotation.ImpactThis resource is now available to the prostate cancer community as a tool for biomarker validation to address important unanswered clinical questions around disease progression and response to treatment.</p

Distribution and molecular detection of apple mosaic virus in apple and hazelnut in Turkey

Apple mosaic virus (ApMV) is one of the most important diseases limiting the production of hazelnut and apple in Turkey and the objectives of this research were to determine the convenient and reliable method for RNA isolation and also to determine primer pair for real time polymerase chain reaction (RT-PCR) detection of coat protein gene for Turkish ApMV isolates. Apple mosaic virus isolates were collected in 2007 to 2010 and the presence of the pathogen was detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and RT-PCR tests. Six different RNA extraction protocols and three primer pairs were applied in RT-PCR amplifications and 44 hazelnut and 15 apple ApMV isolates were obtained. All of the amplicons were subjected to enzymatic digestion with restriction endonuclease enzymes and phylogenetic analysis were performed according to the digestion profiles.Keywords: Apple mosaic virus, coat protein gene, hazelnutAfrican Journal of Biotechnology, Vol 13(31) 3144-314

Molecular variability of zucchini yellow mosaic virus (ZYMV) isolates from Turkey

Cucurbits are widely grown in different provinces of Turkey. However their production is restricted by plant viruses. ZYMV is one of the most economically important viruses of cucurbit crops worldwide, including in Turkey. Several strains have been defined based on their biological, serological and molecular variability –particularly in the coat protein (CP) coding region. One molecular cluster (A1) appears highly prevalent worldwide, but other clusters (A4, A5) have been observed recently in several European and Mediterranean countries, probably in relation to recent introductions. However the situation in Turkey remains unknown. The aim of this research was thus to characterize the molecular variability of ZYMV in Turkey and look for the presence of emerging strains

Batch process monitoring and its application to polymerization systems

Slight changes in raw material properties or operating conditions during critical periods of operation of batch and semi-batch polymerization reactors may have a strong influence on reaction mechanism and impact final product quality. Online process monitoring, fault detection, fault diagnosis, and product quality prediction in real-time ensure safe reactor operation and warn operators about excursions from normal operation that may lead to deterioration in product properties. Multivariate statistical process monitoring and quality prediction using multiway principal components analysis and multiway partial least squares have been successful in detecting abnormalities in process operation and product quality. When abnormal process operation is detected, fault diagnosis tools are used to determine the source cause of the deviation. Illustrative case studies are presented via simulated polyvinyl acetate polymerization.Endnote format citation for DOI:10.1002/masy.20045021

Histopathological changes of cervical tissue in women with systemic sclerosis

PubMedID: 15482565Systemic sclerosis is a connective tissue disease that can affect almost any organ of the body. The clinical aspects of systemic sclerosis on the reproductive system have been studied in large series, and an increased rate of cesarean section has been reported. For this reason, in the present study the histopathological features of cervical specimens of hysterectomyzed women with systemic sclerosis were evaluated. An increased frequency of vascular and stromal abnormalities in cervical specimens of women with systemic sclerosis were observed. Vascular medial hypertrophy, intimal thickening, and fibrosis were more often encountered in the cervical specimens of the patients with systemic sclerosis. Some of the histopathological features also showed correlation with the clinical profile of the disease. The patients with vascular medial hypertrophy in their cervical specimens were older, had a higher Rodnan score, and had longer duration of the disease. In contrast to vascular medial hypertrophy, periadventitial edema was found in the cervical specimens of the patients who were younger, had a lower Rodnan score, and had shorter duration of the disease. It was concluded that the problems that are seen in common obstetric and gynecological practices in patients with systemic sclerosis may be explained by these tissue abnormalities

Identification of different phytoplasmas infecting grapevine in Turkey

Grapevines with severe redness and inward curling of leaves were collected from the main viticulture production areas, Aegean, Central Anatolia and Western Anatolian regions of Turkey. Collected samples were subjected to nucleic acid extraction followed by nested PCR/RFLP analyses that allow to identify different phytoplasmas in symptomatic grapevines. The majority of samples were infected with phytoplasmas belonging to 16SrXII group and referable to the \u2018bois noir\u2019 phytoplasmas, while in some sam-ples 16SrIX or 16SrI-B phytoplasmas were identified. The 16SrIX phytoplasmas are firstly reported in grapevine