249 research outputs found

    Designing multivalent nanoplatforms using antiviral peptides for therapy and diagnosis

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    Mode-hop-free tuning over 135 GHz of external cavity diode lasers without anti-reflection coating

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    We report an external cavity diode laser (ECDL), using a diode whose front facet is not antireflection (AR) coated, that has a mode-hop-free (MHF) tuning range greater than 135 GHz. We achieved this using a short external cavity and by simultaneously tuning the internal and external modes of the laser. We find that the precise location of the pivot point of the grating in our laser is less critical than commonly believed. The general applicability of the method, combined with the compact portable mechanical and electronic design, makes it well suited for both research and industrial applications.Comment: 5 pages, 5 figure

    A Laser System for the Spectroscopy of Highly-Charged Bismuth Ions

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    We present and characterize a laser system for the spectroscopy on highly-charged ^209Bi^82+ ions at a wavelength of 243.87 nm. For absolute frequency stabilization, the laser system is locked to a near-infra-red laser stabilized to a rubidium transition line using a transfer cavity based locking scheme. Tuning of the output frequency with high precision is achieved via a tunable rf offset lock. A sample-and-hold technique gives an extended tuning range of several THz in the UV. This scheme is universally applicable to the stabilization of laser systems at wavelengths not directly accessible to atomic or molecular resonances. We determine the frequency accuracy of the laser system using Doppler-free absorption spectroscopy of Te_2 vapour at 488 nm. Scaled to the target wavelength of 244 nm, we achieve a frequency uncertainty of \sigma_{244nm} = 6.14 MHz (one standard deviation) over six days of operation.Comment: Contribution to the special issue on "Trapped Ions" in "Applied Physics B

    Linewidth of a quantum-cascade laser assessed from its frequency noise spectrum and impact of the current driver

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    We report on the measurement of the frequency noise properties of a 4.6-μm distributed-feedback quantum-cascade laser (QCL) operating in continuous wave near room temperature using a spectroscopic set-up. The flank of the R(14) ro-vibrational absorption line of carbon monoxide at 2196.6cm−1 is used to convert the frequency fluctuations of the laser into intensity fluctuations that are spectrally analyzed. We evaluate the influence of the laser driver on the observed QCL frequency noise and show how only a low-noise driver with a current noise density below {\approx} 1~\mbox{nA/}\sqrt{}\mbox{Hz} allows observing the frequency noise of the laser itself, without any degradation induced by the current source. We also show how the laser FWHM linewidth, extracted from the frequency noise spectrum using a simple formula, can be drastically broadened at a rate of {\approx} 1.6~\mbox{MHz/}(\mbox{nA/}\sqrt{}\mbox{Hz}) for higher current noise densities of the driver. The current noise of commercial QCL drivers can reach several \mbox{nA/}\sqrt{}\mbox{Hz} , leading to a broadening of the linewidth of our QCL of up to several megahertz. To remedy this limitation, we present a low-noise QCL driver with only 350~\mbox{pA/}\sqrt{}\mbox{Hz} current noise, which is suitable to observe the ≈550kHz linewidth of our QC

    The rise of policy coherence for development: a multi-causal approach

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    In recent years policy coherence for development (PCD) has become a key principle in international development debates, and it is likely to become even more relevant in the discussions on the post-2015 sustainable development goals. This article addresses the rise of PCD on the Western donors’ aid agenda. While the concept already appeared in the work of Organisation for Economic Co-operation and Development (OECD) in the early 1990s, it took until 2007 before PCD became one of the Organisation’s key priorities. We adopt a complexity-sensitive perspective, involving a process-tracing analysis and a multi-causal explanatory framework. We argue that the rise of PCD is not as contingent as it looks. While actors such as the EU, the DAC and OECD Secretariat were the ‘active causes’ of the rise of PCD, it is equally important to look at the underlying ‘constitutive causes’ which enabled policy coherence to thrive well

    Stranding of larval nase (Chondrostoma nasus L.) depending on bank slope, down-ramping rate and daytime

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    Rapid water level decreases due to hydropeaking are known to negatively affect riverine biota, mainly due to the stranding of organisms in the river bank area that becomes regularly dewatered. Even though studies of the last decades have focused on salmonid fish, also cyprinids may be affected. However, limited knowledge is available of this fish family. Therefore, we conducted mesocosm experiments under semi-natural conditions, simulating single hydropeaking events at two different lateral bank slopes (2% and 5%) with varying down-ramping rates (0.7–3.0 cm min−1) during day and night. As a response parameter, we quantified stranding rates of different larval stages (III-IV and V) of common nase (Chondrostoma nasus L.). The experiments revealed that lower sloped banks exhibited distinctly higher stranding rates than steeper ones. Daytime revealed a similar pattern, with more fish becoming stranded at night than during the day, and this was consistent for all down-ramping rates. The data also indicate increased stranding with higher down-ramping rates, particularly at low sloped riverbanks, and interaction effects between the tested parameters. Overall, this study, for the first time, quantifies the consequences of flow down-ramping on nase larvae, also revealing differences between larval stages. The gained information will, therefore, advance the ongoing discussion on hydropeaking mitigation by providing a deeper understanding of the effects of artificial sub-daily flow fluctuations on the early life stages of cyprinid fish. Our results can inform management and policy to sharpen existing mitigation concepts and fine-tune hydropower operations to reduce negative effects on riverine ecosystems

    A distinct CD38+CD45RA+ population of CD4+, CD8+, and double-negative T cells is controlled by FAS.

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    The identification and characterization of rare immune cell populations in humans can be facilitated by their growth advantage in the context of specific genetic diseases. Here, we use autoimmune lymphoproliferative syndrome to identify a population of FAS-controlled TCRαβ+ T cells. They include CD4+, CD8+, and double-negative T cells and can be defined by a CD38+CD45RA+T-BET- expression pattern. These unconventional T cells are present in healthy individuals, are generated before birth, are enriched in lymphoid tissue, and do not expand during acute viral infection. They are characterized by a unique molecular signature that is unambiguously different from other known T cell differentiation subsets and independent of CD4 or CD8 expression. Functionally, FAS-controlled T cells represent highly proliferative, noncytotoxic T cells with an IL-10 cytokine bias. Mechanistically, regulation of this physiological population is mediated by FAS and CTLA4 signaling, and its survival is enhanced by mTOR and STAT3 signals. Genetic alterations in these pathways result in expansion of FAS-controlled T cells, which can cause significant lymphoproliferative disease
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