Simultaneous Determination of Pesticides in Fruits by Using Second-Order Fluorescence Data Resolved by Unfolded Partial Least-Squares Coupled to Residual Bilinearization

In the present work, a chemometric-assisted spectrofluorimetric method has been developed for the simultaneous determination of natural fluorescent pesticides, carbaryl, carbendazim, and thiabendazole, in orange and banana. Only a simple extraction with methanol was required as sample pretreatment. Emission-excitation fluorescence matrices were obtained and resolved by using a second-order multivariate calibration method based on unfolded partial least-squares combined with residual bilinearization (U-PLS/RBL) for achieving “second-order advantage.” In this way, pesticides were determined in fruits even in the presence of inner filter effects, background interactions, strong spectral overlapping, and unexpected components. U-PLS can cope with effects that cause trilinearity loss such as, inner filter effects, including background in the calibration set; meanwhile, RBL allows to resolve the presence of unexpected components. The extraction technique was validated against a commonly applied technique based on the use of ethyl acetate and sodium sulfate. Besides, results obtained for real samples were statistically compared with those obtained by using HPLC. LODs of 0.038, 0.054, and 0.018 mg•kg−1 and 0.044, 0.072, and 0.020 mg•kg−1 were obtained for carbaryl, carbendazim, and thiabendazole in banana and orange samples, respectively; values were in accordance with the MRLs (Maximum Residue Limits) established by different official control organizations such as National Food Safety and Quality Service (SENASA), Codex Alimentarius (based on Food and Agriculture Organization (FAO) of the United Nations and World Health Organization (WHO), and Environmental Protection Agency (EPA).National University of Rosario, Faculty of Biochemical and Pharmaceutical Sciences, Rosario, Argentina BIO 253 BIO 415Argentine Catholic University, Faculty of Chemistry and Engineering, Rosario, ArgentinaCONICET (National Centre of Scientific and Technical Research)ANPCyT (National Agency of Scientific and Technological Promotion

Protocol Proposal For The Care Of The Person With Venous Ulcer

Objective: To propose a care protocol for the care of the person with a venous ulcer in highly complex services. Methods and results: This is a methodological study, in three stages: literature review, validation of content and validation in the clinical context. The literature review was carried out from June to August/2011, being the basis for the construction of the Protocol for Venous Ulcers. The content validation included 53 judges (44 nurses, 8 physicians and 1 physiotherapist) selected through the Lattes platform to evaluate the items of the protocol. Validation in the clinical context occurred at the University Hospital Onofre Lopes, in Natal/RN with four judges (nurses), who worked in pairs, evaluating 32 patients with venous ulcers. The protocol was validated with 15 categories: sociodemographic data; anamnesis; examinations; ulcer characteristics; care with the lesion and perilesional area; medicines used to treat the lesion; evaluation and treatment of pain; surgical treatment of chronic venous disease; recurrence prevention (clinical and educational strategies); reference; counter-reference; and quality of life. Conclusion: The validated protocol regarding content and clinical context was applicable. Its implementation is a viable measure that assists in the reorientation of the team in high complexity services, aiming at wound healing and restoration of the patient´s integral health. Keywords: Varicose ulcer; Tertiary Health Care; Protocols; Validation studies

Núcleos de Ensino da Unesp: artigos 2007

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Núcleos de Ensino da Unesp: artigos 2008

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

NEOTROPICAL ALIEN MAMMALS: a data set of occurrence and abundance of alien mammals in the Neotropics

Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal-central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus, Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. (n = 37,782), Sus scrofa (n = 6,730), and Canis familiaris (n = 10,084); 17 species were represented by only one record (e.g., Syncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans). Primates have the highest number of species in the data set (n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata, and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation-related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data