1,981 research outputs found
Articular cartilage collagen: an irreplaceable framework?
Adult articular cartilage by dry weight is two-thirds collagen. The collagen has a unique molecular phenotype. The nascent type II collagen fibril is a heteropolymer, with collagen IX molecules covalently linked to the surface and collagen XI forming the filamentous template of the fibril as a whole. The functions of collagens IX and XI in the heteropolymer are far from clear but, evidently, they are critically important since mutations in COLIX and COLXI genes can result in chondrodysplasia syndromes. Here we review what is known of the collagen assembly and present new evidence that collagen type III becomes covalently added to the polymeric fabric of adult human articular cartilage, perhaps as part of a matrix repair or remodelling process
A novel approach for exploring climatic factors limiting current pest distributions: A case study of Bemisia tabaci in north-west Europe and assessment of potential future establishment in the United Kingdom under climate change
This is the final version. Available on open access from Public Library of Science via the DOI in this recordData Availability: All relevant data are within the manuscript and its Supporting Information files.Bemisia tabaci (the tobacco whitefly) is an important agricultural pest of global significance primarily because of its ability to transmit multiple damaging plant viruses. To date, UK outbreaks of the whitefly have been restricted to glasshouses and there are no records of the whitefly establishing outdoors during the summer. This is despite the fact that annual degree-day models (that estimate accumulated warmth over the year above the development threshold), indicate that B. tabaci has the thermal potential for multiple summer generations in the UK. A set of 49 climate indices calculated using the present day climate (1986â2015) were therefore compared between the UK and the south of France, where B. tabaci is able to establish outdoors, to identify the factors limiting its establishment. The number of cold days and nights in summer, as well as the time spent within the whiteflyâs optimum temperature range, were most significantly different between the two areas. These indices may impact the development of B. tabaci and offer an explanation for the absence of the whitefly outdoors in the UK during the summer. Further analyses undertaken with climate projections suggest that in a 2â4°C warmer world this pest could pose a risk to outdoor UK crops in July and August. A clear south-north gradient can be demonstrated for these indices. Linking any possible northwards spread of B. tabaci populations outdoors in France with changes in these indices could therefore provide an important indicator of any change in the risks of outdoor populations of this species developing in the UK. The effectiveness of climate indices in pest risk analysis is compellingly demonstrated, and it is recommended that in-depth comparisons of climatic indices between areas of pest presence and absence are conducted in other situations where forecasting the risks of pest establishment are complex and challenging.Joint UK BEIS/Defra Met Office Hadley Centre Climate Programm
Hysteresis Between Coral Reef Calcification and the Seawater Aragonite Saturation State
Some predictions of how ocean acidification (OA) will affect coral reefs assume a linear functional relationship between the ambient seawater aragonite saturation state (Ωa) and net ecosystem calcification (NEC). We quantified NEC in a healthy coral reef lagoon in the Great Barrier Reef during different times of the day. Our observations revealed a diel hysteresis pattern in the NEC versus Ωa relationship, with peak NEC rates occurring before the Ωa peak and relatively steady nighttime NEC in spite of variable Ωa. Net ecosystem production had stronger correlations with NEC than light, temperature, nutrients, pH, and Ωa. The observed hysteresis may represent an overlooked challenge for predicting the effects of OA on coral reefs. If widespread, the hysteresis could prevent the use of a linear extrapolation to determine critical Ωa threshold levels required to shift coral reefs from a net calcifying to a net dissolving state
Groundwater and Porewater as Major Sources of Alkalinity to a Fringing Coral Reef Lagoon (Muri Lagoon, Cook Islands)
To better predict how ocean acidification will affect coral reefs, it is important to understand how biogeochemical cycles on reefs alter carbonate chemistry over various temporal and spatial scales. This study quantifies the contribution of shallow porewater exchange (as quantified from advective chamber incubations) and fresh groundwater discharge (as traced by 222Rn) to total alkalinity (TA) dynamics on a fringing coral reef lagoon along the southern Pacific island of Rarotonga over a tidal and diel cycle. Benthic alkalinity fluxes were affected by the advective circulation of water through permeable sediments, with net daily flux rates of carbonate alkalinity ranging from â1.55 to 7.76 mmol mâ2 dâ1, depending on the advection rate. Submarine groundwater discharge (SGD) was a source of TA to the lagoon, with the highest flux rates measured at low tide, and an average daily TA flux of 1080 mmol mâ2 dâ1 at the sampling site. Both sources of TA were important on a reef-wide basis, although SGD acted solely as a delivery mechanism of TA to the lagoon, while porewater advection was either a sink or source of TA dependent on the time of day. This study describes overlooked sources of TA to coral reef ecosystems that can potentially alter water column carbonate chemistry. We suggest that porewater and groundwater fluxes of TA should be taken into account in ocean acidification models in order to properly address changing carbonate chemistry within coral reef ecosystems
Assessment of the ergogenic effect of caffeine supplementation on mood, anticipation timing, and muscular strength in older adults
The effect of caffeine to promote improvements in mood, cognition, and exercise performance has been well established in young and athletic adults. However, little is known about whether such nutritional ergogenic aids are effective in enhancing psychological well-being, physiological or cognitive performance in older adults. This study assesses the ergogenic effect of caffeine on mood, perceptual-motor coupling, and muscular strength in an older human population. Following a familiarization session, 12 apparently healthy volunteers (nine females and three males; 69 ± 6 years) completed two laboratory visits. "Pre ingestion" trials of mood state Brunel Mood State Inventory (BRUMS) and coincidence anticipation performance (Bassin anticipation timer) at slow (3 mph) and fast (8 mph) stimulus speeds were completed on both visits. Using a randomized, double-blind, cross-over design, participants consumed either caffeine (3 mg/kg body mass) or a placebo. Sixty minutes postingestion participants repeated the trials before completing a set of 10 consecutive repetitions of maximal knee extension using isokinetic dynamometry. Rating of perceived exertion (RPE) was assessed following the fifth and final repetition. Caffeine ingestion significantly improved mood state scores for vigor by 17% (P = 0.009) and reduced absolute error by 35% (P = 0.045) during coincidence anticipation assessment at 8 mph compared to placebo. There were no other significant effects. Caffeine ingestion failed to augment maximal voluntary contraction of the knee extensors and RPE did not prove to be significantly different to from placebo (P > 0.33 in each case). Acute caffeine ingestion may not be an effective ergogenic aid for improving muscular strength in older adults but could possibly be used as a nutrition supplement for enhancing mood and improving cognitive performance in daily living tasks where interceptive timing skills are required
Carbon Cycling Hysteresis in Permeable Carbonate Sands over a Diel Cycle: Implications for Ocean Acidification
Dissolved inorganic carbon, dissolved oxygen, H+, and alkalinity fluxes from permeable carbonate sediments at Heron Island (Great Barrier Reef) were measured over one diel cycle using benthic chambers designed to induce advective poreâwater exchange. A complex hysteretic pattern between carbonate precipitation and dissolution in sands and the aragonite saturation state (ΩAr) of the overlying chamber water was observed throughout the incubations. During the day, precipitation followed a hysteretic pattern based on the incidence of photosynthetically active radiation with lower precipitation rates in the morning than in the afternoon. The observed diel hysteresis seems to reflect a complex interaction between photosynthesis and respiration rather than ΩAr of the overlying water as the main driver of carbonate precipitation and dissolution within these permeable sediments. Changes in flux rates over a diel cycle demonstrate the importance of taking into account the shortâterm variability of benthic metabolism when calculating net daily flux rates. Based on one diel cycle, the sediments were a net daily source of alkalinity to the water column (5.13 to 8.84 mmol mâ2 dâ1, depending on advection rates), and advection had a net stimulatory effect on carbonate dissolution. The enhanced alkalinity release associated with benthic metabolism and poreâwater advection may partially buffer shallow coral reef ecosystems against ocean acidification on a local scale
Types I and II collagens in intervertebral disc. Interchanging radial distributions in annulus fibrosus
The distribution of different molecular species of collagen in fibrous, elastic and hyaline cartilages of the pig
Increase in degraded collagen type II in synovial fluid early in the rabbit meniscectomy model of osteoarthritis
SummaryObjectiveThe objective of this study was to determine whether collagen type II breakdown products in synovial fluid (SF), detected by an enzyme-linked immunoassay, represent a useful marker for early events in osteoarthritis (OA) in the rabbit medial meniscectomy model.DesignComplete medial meniscectomy was performed on the right knee joints of 32 rabbits. Balanced groups of rabbits were then sacrificed at 2, 4, 8, and 12 weeks post-surgery. An additional 8 unoperated and 11 sham-operated animals served as controls. SF lavages were performed on right and left knee joints of the same animals at sacrifice. The proteolytic epitope of type II collagen was monitored using an enzyme-linked immunoassay.ResultsMacroscopically visible surface fibrillation and focal erosions appeared as early as 2 weeks after meniscectomy in the femorotibial joint (P<0.01). OA developed gradually during the later observation period, and then predominantly on the medial tibial plateau and medial femur. Significant histological alterations in cartilage, including a loss of proteoglycans, surface irregularities, and clefts, were detected at 2 weeks after meniscectomy (P<0.01). Collagen type II epitope levels in SF lavage samples were elevated peaking at 2 weeks after meniscectomy (P<0.02). Levels decreased at later time points, but they were still raised at 12 weeks (Pâ€0.05). Highly significant correlations were found between the SF collagen type II epitope levels and the macroscopic and microscopic scoring results (Spearman rho correlation coefficient, macroscopyâcollagen type II epitope r=0.222, P=0.025; microscopyâcollagen type II epitope r=0.436, Pâ€0.01).ConclusionIn this rabbit model of medial meniscectomy, levels of type II collagen fragments in SF appear to provide a useful marker of the early degenerative changes
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