Międzynarodowa konferencja "Dzieło Oskara Kolberga jako dziedzictwo narodowe i europejskie"

"Honorowy patronat nad konferencją objął Prezydent Rzeczypospolitej Polskiej Bronisław Komorowski. Została ona zorganizowana przez Instytut im. Oskara Kolberga w Poznaniu we współpracy z Komitetem Nauk Etnologicznych PAN, Instytutem Etnologii i Antropologii Kulturowej UAM i Polskim Towarzystwem Ludoznawczym, przy fi nansowym wsparciu Ministerstwa Nauki i Szkolnictwa Wyższego, a także dotacji Urzędu Marszałkowskiego Województwa Wielkopolskiego." [...] (fragm.

Low-vacuum filtration as an alternative extracellular vesicle concentration method : a comparison with ultracentrifugation and differential centrifugation

Recent years have brought great focus on the development of drug delivery systems based on extracellular vesicles (EVs). Considering the possible applications of EVs as drug carriers, the isolation process is a crucial step. To solve the problems involved in EV isolation, we developed and validated a new EV isolation method—low-vacuum filtration (LVF)—and compared it with two commonly applied procedures—differential centrifugation (DC) and ultracentrifugation (UC). EVs isolated from endothelial cell culture media were characterized by (a) Transmission Electron Microscopy (TEM), (b) Nanoparticle Tracking Analysis (NTA), (c) Western blot and (d) Attenuated Total Reflection Fourier-Transform Infrared Spectroscopy (ATR-FTIR). Additionally, the membrane surface was imaged with Environmental Scanning Electron Microscopy (ESEM). We found that LVF was a reproducible and efficient method for EV isolation from conditioned media. Additionally, we observed a correlation between ATR-FTIR spectra quality and EV and protein concentration. ESEM imaging confirmed that the actual pore diameter was close to the values calculated theoretically. LVF is an easy, fast and inexpensive EV isolation method that allows for the isolation of both ectosomes and exosomes from high-volume sources with good repeatability. We believe that it could be an efficient alternative to commonly applied methods

Stan polskiej gospodarki żywnościowej po przystąpieniu do Unii Europejskiej. Raport 5

Seria: Program Wieloletni 2005-2009. Ekonomiczne i Społeczne Uwarunkowania Rozwoju Polskiej Gospodarki Żywnościowej po Wstąpieniu Polski do Unii Europejskiej, nr 109Makroekonomiczne i rynkowe uwarunkowania rozwoju polskiej gospodarki żywnościowej: wskaźniki makroekonomiczne, ceny, wsparcie finansowe. Popyt na żywność i produkty rolno-spożywcze. Rozwój produkcji i przemiany strukturalne w rolnictwie i przemyśle spożywczym: produkcja rolnictwa, produkcja przetwórstwa, globalizacja, przemiany strukturalne. Wyniki i sytuacja ekonomiczno-finansowa rolnictwa i przemysłu spożywczego: przychody, zaopatrzenie w środki produkcji, sytuacja przedsiębiorstw rolnych, sytuacja przedsiębiorstw usługowych, sytuacja przemysłu spożywczego.Kamila Sobieck

Relationship between echocardiographic parameters and exercise test duration in patients after myocardial infarction

Background: The interdependence between echocardiographic parameters of left ventricle function, severity of heart failure (HF) and exercise test duration has not been thoroughly examined. Methods: We compared echocardiographic data in patients after myocardial infarction divided according to NYHA class to mild (class I and II, group 1 = 24 subjects) and advanced HF group (III and IV, group 2 = 36) and assessed their correlation with exercise duration (ED) in a symptom-limited treadmill test. Then we tried to determine independent predictors of ED. Results: The group with advanced HF had lower left ventricle ejection fraction, shorter duration and deceleration times of both mitral inflow waves (Et and At, Edt and Adt) and higher E/A ratio (1.4 &#177; 1.1 vs. 0.9 &#177; 0.4; p < 0.05) with more frequent restriction and pseudonormalization pattern (56% vs. 12%). Also early wave propagation (21 &#177; 7 vs. 29 &#177; 11 cm/s; p < 0.001) and all tissue Doppler velocities were lower, but ratio of early wave peak velocity to early diastolic velocity of mitral annulus was higher (E/E&#8217; 10.5 &#177; 5 vs. 6.1 &#177; 1.3 for velocity ratio; p < 0.001) in subjects with more severe clinical symptoms. Significant negative correlation with ED was observed for difference between duration of pulmonary vein atrial reversal flow and atrial wave of mitral inflow (&#8710;At; r = &#8211;0.54) and for E/E&#8217; ratio (r = &#8211;0.48), the highest positive correlation for left ventricular ejection fraction and duration of mitral inflow atrial phase (EF; r = 0.48, At; r = 0.46). In multivariate stepwise regression analysis two independent predictors of ED were identified: age and &#8710;At (Art&#8211;At). Conclusions: Diastolic parameters showing the strongest correlation with ED (&#8710;At and E/E&#8217;) are connected with restrictive left ventricle physiology. The only independent predictors of exercise duration in patients after myocardial infarction were: age and &#8710;At

Relationship between the CHA2DS2-VASc score and atrial fibrillation in patients hospitalised due to heart failure

Background: Heart failure (HF) has become an epidemic. A similar situation is also observed for atrial fibrillation (AF). The  CHA2DS2-VASc score is one of the most useful tools for thromboembolic risk assessment.  Aims: The aim of the study was to assess the prevalence of AF in patients with decompensated HF, who were divided into subgroups according to the CHA2DS2-VASc score.  Methods: We analysed the prevalence of AF in a group of 1108 patients (327 women) hospitalised due to HF decompensa- tion in medical centres of different referral levels. Twenty-one patients refused to participate in the registry. The data were collected from Polish centres included in the European Society of Cardiology Heart Failure Long-Term Registry. The recruit- ment period was from 2011 to 2014. The data were analysed retrospectively. Patients were divided into groups according to the CHA2DS2-VASC score.  Results: The study sample was characterised by a high occurrence of AF (44.3%), with the highest prevalence in patients with a CHA2DS2-VASC score ≥ 6 (61.3%, p = 0.01).  Conclusions: The CHA2DS2-VASc score may be a useful tool for detecting patients with HF characterised by the highest risk of AF.