Processes underlying the nutritional programming of embryonic development by iron deficiency in the rat

Peer reviewedPublisher PD

The massive multiple system HD 64315

The O6 Vn star HD 64315 is believed to belong to the star-forming region known as NGC 2467, but previous distance estimates do not support this association. We explore the multiple nature of this star with the aim of determining its distance, and understanding its connection to NGC 2467. A total of 52 high-resolution spectra have been gathered over a decade. We use their analysis, in combination with the photometric data from All Sky Automated Survey and Hipparcos catalogues, to conclude that HD 64315 is composed of at least two spectroscopic binaries, one of which is an eclipsing binary. HD 64315 contains two binary systems, one of which is an eclipsing binary. The two binaries are separated by 0.09 arcsec (or 500 AU) if the most likely distance to the system, around 5 kpc, is considered. The presence of fainter companions is not excluded by current observations. The non-eclipsing binary (HD 64315 AaAb) has a period of 2.70962901+/-0.00000021 d. Its components are hotter than those of the eclipsing binary, and dominate the appearance of the system. The eclipsing binary (HD 64315 BaBb) has a shorter period of 1.0189569+/-0.0000008 d. We derive masses of 14.6+-2.3 M$_\odot$ for both components of the BaBb system. They are almost identical; both stars are overfilling their respective Roche lobes, and share a common envelope in an overcontact configuration. The non-eclipsing binary is a detached system composed of two stars with spectral types around O6 V with minimum masses of 10.8 M$_\odot$ and 10.2 M$_\odot$, and likely masses aprox. 30 M$_\odot$. HD 64315 provides a cautionary tale about high-mass star isolation and multiplicity. Its total mass is likely above 90 M$_\odot$,but it seems to have formed without an accompanying cluster. It contains one the most massive overcontact binaries known, a likely merger progenitor in a very wide multiple system.Comment: 14 pages, 13 figures, 8 Table

Body composition and behaviour in adult rats are influenced by maternal diet, maternal age and high-fat feeding

Fetal exposure to maternal undernutrition has lifelong consequences for physiological and metabolic function. Maternal low-protein diet is associated with an age-related phenotype in rats, characterised by a period of resistance to development of obesity in early adulthood, giving way to an obesity-prone, insulin-resistant state in later adulthood. Offspring of rats fed a control (18 % casein) or low-protein (9 % casein; LP) diet in pregnancy were challenged with a high-fat diet at 9 months of age. To assess whether other maternal factors modulated the programming effects of nutrition, offspring were studied from young (2–4 months old) and older (6–9 months old) mothers. Weight gain with a high-fat diet was attenuated in male offspring of older mothers fed LP (interaction of maternal age and diet; P = 0·011) and adipose tissue deposition was lower with LP feeding in both males and females (P < 0·05). Although the resistance to weight gain and adiposity was partially explained by lower energy intake in offspring of LP mothers (P < 0·001 males only), it was apparent that energy expenditure must be influenced by maternal diet and age. Assessment of locomotor activity indicated that energy expenditure associated with physical activity was unlikely to explain resistance to weight gain, but showed that offspring of older mothers were more anxious than thoseof younger mothers, with more rearing observed in a novel environment and on the elevated plus-maze. The data showed that in addition to maternal undernutrition, greater maternal age may influence development and long-term body composition in the rat

The impact of maternal protein restriction during rat pregnancy upon renal expression of angiotensin receptors and vasopressin-related aquaporins

<p>Abstract</p> <p>Background</p> <p>Maternal protein restriction during rat pregnancy is known to impact upon fetal development, growth and risk of disease in later life. It is of interest to understand how protein undernutrition influences the normal maternal adaptation to pregnancy. Here we investigated the mechanisms regulating renal haemodynamics and plasma volume during pregnancy, in the context of both normal and reduced plasma volume expansion. The study focused on expression of renal angiotensin receptors (ATR) and vasopressin-related aquaporins (AQP), hypothesising that an alteration in the balance of these proteins would be associated with pregnancy <it>per se </it>and with compromised plasma volume expansion in rats fed a low-protein diet.</p> <p>Methods</p> <p>Female Wistar rats were mated and fed a control (18% casein) or low-protein (9% casein) diet during pregnancy. Animals were anaesthetised on days 5, 10, 15 and 20 of gestation (n = 8/group/time-point) for determination of plasma volume using Evans Blue dye, prior to euthanasia and collection of tissues. Expression of the ATR subtypes and AQP2, 3 and 4 were assessed in maternal kidneys by PCR and western blotting. 24 non-pregnant Wistar rats underwent the same procedure at defined points of the oestrous cycle.</p> <p>Results</p> <p>As expected, pregnancy was associated with an increase in blood volume and haemodilution impacted upon red blood cell counts and haemoglobin concentrations. Expression of angiotensin II receptors and aquaporins 2, 3 and 4 was stable across all stages of the oestrus cycle. Interesting patterns of intra-renal protein expression were observed in response to pregnancy, including a significant down-regulation of AQP2. In contrast to previous literature and despite an apparent delay in blood volume expansion in low-protein fed rats, blood volume did not differ significantly between groups of pregnant animals. However, a significant down-regulation of AT₂R protein expression was observed in low-protein fed animals alongside a decrease in creatinine clearance.</p> <p>Conclusion</p> <p>Regulatory systems involved in the pregnancy-induced plasma volume expansion are susceptible to the effects of maternal protein restriction.</p

Early life programming of health and disease: The long-term consequences of obesity in pregnancy

The prevalence of overweight and obesity is rising in all parts of the world and, among young women, it presents a very clear danger during pregnancy. Women who are overweight or who gain excessive weight during pregnancy are at greater risk of complications in pregnancy and labour, and are more likely to lose their child to stillbirth or die themselves during pregnancy. This narrative review considers the evidence that, in addition to increasing risk of poor pregnancy outcomes, obesity has the capacity to programme foetuses to be at greater risk of cardiometabolic disorders later in life. An extensive body of evidence from prospective and retrospective cohorts, as well as record linkage studies, demonstrates associations of maternal obesity and/or gestational diabetes with cardiovascular disease, as well as type 1 and type 2 diabetes. Studies in animals suggest that these associations are underpinned by adaptations that occur in foetal life, which remodel the structures of major organs, including the brain, kidney and pancreas

Solution phase, solid state, and theoretical investigations on the MacMillan imidazolidinone

A combination of soln. phase NMR, X-ray crystallog. studies, and DFT calcns. provide a consistent structural conformation for iminium ions derived from the MacMillan imidazolidinone

Setting targets leads to greater long-term weight losses and ‘unrealistic’ targets increase the effect in a large community-based commercial weight management group

Background. Setting personal targets is an important behavioural component in weight management programmes. Normal practice is to encourage ‘realistic’ weight loss but the under-pinning evidence base for this is limited and controversial. This study investigates the effect of number and size of weight loss targets on long-term weight loss in a large community sample of adults. Methods. Weight change, attendance and target weight data for all new UK members, joining January to March 2012 was extracted from a commercial slimming organisation’s electronic database. Results. Of the 35 380 members who had weight data available at 12 months after joining, 69.1% (n=24 447)had a starting BMI≥30kg/m2. Their mean weight loss was 12.9±7.8% and for both sexes, weight loss at 12 months was greater for those who set targets (p25% was 7.6±4.0 kg/m2. A higher percentage of obese members did not set targets (p<0.001) compared to those with a BMI below 30kg/m2. Conclusions. Much of the variance in achieved weight loss in this population was explained by the number of targets set and the size of the first target. Whilst obese people were less likely to set targets, doing so increased the likelihood of achieving clinically significant weight loss and for some ‘unrealistic’ targets improved results

Asymmetric quantum error correction via code conversion

In many physical systems it is expected that environmental decoherence will exhibit an asymmetry between dephasing and relaxation that may result in qubits experiencing discrete phase errors more frequently than discrete bit errors. In the presence of such an error asymmetry, an appropriately asymmetric quantum code - that is, a code that can correct more phase errors than bit errors - will be more efficient than a traditional, symmetric quantum code. Here we construct fault tolerant circuits to convert between an asymmetric subsystem code and a symmetric subsystem code. We show that, for a moderate error asymmetry, the failure rate of a logical circuit can be reduced by using a combined symmetric asymmetric system and that doing so does not preclude universality.Comment: 5 pages, 8 figures, presentation revised, figures and references adde

The Effects of Prenatal Protein Restriction on β-Adrenergic Signalling of the Adult Rat Heart during Ischaemia Reperfusion

A maternal low-protein diet (MLP) fed during pregnancy leads to hypertension in adult rat offspring. Hypertension is a major risk factor for ischaemic heart disease. This study examined the capacity of hearts from MLP-exposed offspring to recover from myocardial ischaemia-reperfusion (IR) and related this to cardiac expression of β-adrenergic receptors (β-AR) and their associated G proteins. Pregnant rats were fed control (CON) or MLP diets (n = 12 each group) throughout pregnancy. When aged 6 months, hearts from offspring underwent Langendorff cannulation to assess contractile function during baseline perfusion, 30 min ischemia and 60 min reperfusion. CON male hearts demonstrated impaired recovery in left ventricular pressure (LVP) and dP/dtmax (P < 0.01) during reperfusion when compared to MLP male hearts. Maternal diet had no effect on female hearts to recover from IR. MLP males exhibited greater membrane expression of β2-AR following reperfusion and urinary excretion of noradrenaline and dopamine was lower in MLP and CON female rats versus CON males. In conclusion, the improved cardiac recovery in MLP male offspring following IR was attributed to greater membrane expression of β2-AR and reduced noradrenaline and dopamine levels. In contrast, females exhibiting both decreased membrane expression of β2-AR and catecholamine levels were protected from IR injury

Human Resource Needs and Demand for Post-Secondary Education in the Canadian Secondary Wood Products Industry

Skilled labor shortages in manufacturing industries are being reported in a number of countries. The extent to which such shortages are affecting Canadian wood manufacturing industries is not known. The aim of this study was to survey the skills and educational needs of Canadian wood manufacturing industries, the status, capacity, and challenges that post-secondary education institutes face in meeting industries' human resource needs, and finally the attractiveness of the industry to high school job-seekers. The majority (83%) of wood manufacturing companies in Canada are experiencing problems in hiring skilled tradespeople, and 54.5% of the companies face difficulties in hiring and retaining professionals and supervisory personnel. Skilled staff shortages and competitive pressures were nominated by companies as the two most important factors restricting their growth. The skills needs of companies have changed over the last 10 years, and companies now place a much higher premium on leadership and communication skills from management, and attitude and advanced technical skills from tradespeople. Most companies offered some kind of in-house training to redress skills gaps, but less than half were likely to use the Internet to deliver in-house training. The majority of high school students were not interested in pursuing a career in the wood manufacturing industry because of its association with unsustainable forestry practices and manual labor, and the availability of more attractive career options. Lack of student interest in wood manufacturing is affecting the institutions offering relevant vocational and professional training, and nine of the thirteen institutes suffer from under-enrollment in their wood manufacturing programs. Comprehensive strategies are required to address the labor market imbalances currently affecting Canada's wood manufacturing industries