Accurate and precise viral quantification for rapid vaccine development in- process production monitoring using Radiance® Laser Force Cytology\u3csup\u3eTM

The biopharmaceutical world is evolving rapidly, bringing with it the need for technologies to support this fast-paced and changing environment. Trends in biomanufacturing are moving towards shortened development cycles as companies balance increased productivity requirements with the goal of reducing costs while at the same time ensuring production consistencies are met and batch out of specification (OOS) and failure events are minimized. LumaCyte’s Radiance® instrument using Laser Force Cytology™ (LFC), a combination of advanced optics and microfluidics to rapidly analyze single cells based upon their intrinsic biochemical and biophysical cellular properties and without the need for antibodies or labels. Subtle cellular changes can be precisely captured with Radiance’s automated workflow enabling new capabilities for measuring real-time product quality attributes to support R&D, process development and manufacturing needs across the biopharmaceutical industry. In this poster, LumaCyte demonstrates how tedious infectivity assays such as plaque and TCID50 can be replaced by Radiance’s rapid viral infectivity quantification assay to provide significant shorter time to result (TTR), reduced labor, and improved data quality and consistency. In addition, the bioproduction of vaccines, viral vectors or VLPs can be monitored in real-time, enabling rapid optimization of key processes and increasing process knowledge. As a result, product yield can be increased using the same inputs and the likelihood of OOS events can be reduced. Radiance applications in oncolytic virus research and neutralization assays are presented as well. Overall, LFC delivers faster TTR and improved data quality for vaccine analytics from R&D to manufacturing

Laser force cytology for rapid quantification of viral infectivity

The quantification of viral infectivity is an integral step at multiple stages in the process of virally producing recombinant protein, studying the mechanism of viral infection, and developing vaccines. Accurate measurements of infectivity allow for consistent infection and expansion, maximum yield, and assurance that time or environmental conditions have not degraded product quality. Traditional methods to assess infectivity, including the end-point dilution assay (TCID50) and viral plaque assay, are slow, labor intensive, and can vary depending upon the skill and experience of the user. Application of Laser Force Cytology (LFC) for the rapid detection and quantification of viral infection will be presented and discussed for several viral systems in the context of improving the development and production of vaccines. LumaCyte’s Radiance™ instrument is an automated cell analyzer and sorter that measures the optical force, size, shape, and deformability and captures images of single cells. By measuring the intrinsic properties of single cells, cellular changes due to viral infection can be rapidly and objectively quantitated. LFC is very sensitive to agents that perturb cellular structures or change biochemical composition. High quality viral infectivity measurements can be made in a fraction of the time, labor, and cost of traditional assays such as plaque or endpoint dilution. For in-process automated bioreactor monitoring, infectivity can be measured by Radiance in near real-time throughout the process, allowing critical feedback control and optimization. The measurement speed and data quality of LFC / Radiance serve to enhance vaccine development, process optimization/scale-up, and manufacturing to ultimately improve the delivery of vaccines to patients

Kontrol Diri Dan Kecemasan Komunikasi Interpersonal Pada Pramuwisata

This study aim to analysis relationship of self-control and anxiety of interpersonal communicationin a tourist guide. Product moment corelation analysis (rxy) yields for 0905, to provethe relationship between self-control and interpersonal communication anxiety in a tourist guide.These results indicate a negative relationship between the variables X (Self Control) and variableY (Interpersonal Communication Anxiety in the guides). Social Learning Theory became atheories used in this study, where most of the individual behavior acquired partly the result oflearning through observation of behavior displayed other individuals whose became the model.Elaboration Likelihood Theory which states that each individual will interpret the message orinformation they receive, in accordance with the information they have and their beliefs aboutsomething related to the message. Self-control on the tour guides in Yogyakarta based on thefive major aspects of self-control, that has a very high level of measurement. Includes the abilityto anticipate events, the ability to interpret events and the ability to take decisions. Meanwhile,two other aspects of self control shows the results of measurements at very low category, whichconsists of aspects of behavior and stimulus control. While anxiety based on the three aspects ofinterpersonal communication, which consists of inhibition of the ability to express themselves,lack of interest in communicating and social interaction is interrupted, it indicates the levelmeasurement at very low category. Thus, interpersonal communication anxiety on tour guidesin Yogyakarta can be said to be very low

Long-Ranged Correlations in Sheared Fluids

The presence of long-ranged correlations in a fluid undergoing uniform shear flow is investigated. An exact relation between the density autocorrelation function and the density-mometum correlation function implies that the former must decay more rapidly than $1/r$, in contrast to predictions of simple mode coupling theory. Analytic and numerical evaluation of a non-perturbative mode-coupling model confirms a crossover from $1/r$ behavior at ''small'' $r$ to a stronger asymptotic power-law decay. The characteristic length scale is $\ell \approx \sqrt{\lambda_{0}/a}$ where $$ is the sound damping constant and $a$ is the shear rate.Comment: 15 pages, 2 figures. Submitted to PR

Planning for Sustainability in Small Municipalities: The Influence of Interest Groups, Growth Patterns, and Institutional Characteristics

How and why small municipalities promote sustainability through planning efforts is poorly understood. We analyzed ordinances in 451 Maine municipalities and tested theories of policy adoption using regression analysis.We found that smaller communities do adopt programs that contribute to sustainability relevant to their scale and context. In line with the political market theory, we found that municipalities with strong environmental interests, higher growth, and more formal governments were more likely to adopt these policies. Consideration of context and capacity in planning for sustainability will help planners better identify and benefit from collaboration, training, and outreach opportunities

Moyal star product approach to the Bohr-Sommerfeld approximation

The Bohr-Sommerfeld approximation to the eigenvalues of a one-dimensional quantum Hamiltonian is derived through order $\hbar^2$ (i.e., including the first correction term beyond the usual result) by means of the Moyal star product. The Hamiltonian need only have a Weyl transform (or symbol) that is a power series in $\hbar$, starting with $\hbar^0$, with a generic fixed point in phase space. The Hamiltonian is not restricted to the kinetic-plus-potential form. The method involves transforming the Hamiltonian to a normal form, in which it becomes a function of the harmonic oscillator Hamiltonian. Diagrammatic and other techniques with potential applications to other normal form problems are presented for manipulating higher order terms in the Moyal series.Comment: 27 pages, no figure

Chaotic hypothesis: Onsager reciprocity and fluctuation-dissipation theorem

It is shown that the "chaoticity hypothesis", analogous to Ruelle's principle for turbulence and recently introduced in statistical mechanics, implies the Onsager reciprocity and the fluctuation dissipation theorem in various models for coexisting transport phenomena.Comment: 16 pages, postscrip

Edge-control and surface-smoothness in sub-aperture polishing of mirror segments

This paper addresses two challenges in establishing a new process chain for polishing hexagonal segments for extremely large telescopes:- i) control of edge and corner profiles in small-tool polishing of hexagons, and ii) achieving the required smoothness of the bulk aspheric form. We briefly describe the performance of a CNC-grinding process used to create the off-axis asphere, which established the input-quality for subsequent processing. We then summarize processes for smoothing ground mid-spatials and pre- and corrective polishing using Zeeko CNC machines. The impact of two cases is considered; i) all processing stages are performed after the segment is cut hexagonal, and ii) final rectification of a hexagon after cutting from an aspherised roundel, as an alternative to ionfiguring. We then report on experimental results on witness samples demonstrating edges and corners close to the EELT segment specification, and results on a full-aperture spherical segment showing excellent surface smoothness. © 2012 SPIE

The Complement of Projection Neurons Activated Determines the Type of Feeding Motor Program in Aplysia

Multiple projection neurons are often activated to initiate behavior. A question that then arises is, what is the unique functional role of each neuron activated? We address this issue in the feeding system of Aplysia. Previous experiments identified a projection neuron [cerebral buccal interneuron 2 (CBI-2)] that can trigger ingestive motor programs but only after it is repeatedly stimulated, i.e., initial programs are poorly defined. As CBI-2 stimulation continues, programs become progressively more ingestive (repetition priming occurs). This priming results, at least in part, from persistent actions of peptide cotransmitters released from CBI-2. We now show that in some preparations repetition priming does not occur. There is no clear seasonal effect; priming and non-priming preparations are encountered throughout the year. CBI-2 is electrically coupled to a second projection neuron, cerebral buccal interneuron 3 (CBI-3). In preparations in which priming does not occur, we show that ingestive activity is generated when CBI-2 and CBI-3 are coactivated. Programs are immediately ingestive, i.e., priming is not necessary, and a persistent state is not induced. Our data suggest that dynamic changes in the configuration of activity can vary and be determined by the complement of projection neurons that trigger activity

Use of Antiviral Drugs to Reduce Household Transmission of Pandemic (H1N1) 2009, United Kingdom1

TOC Summary: Early treatment of primary case-patients and prophylaxis of household contacts provides effective protection