Recent advances in production of lignocellulolytic enzymes by solid-state fermentation of agro-industrial wastes

Agricultural, forestry, and food industries produce large amounts of lignocellulosic wastes every year. Land disposal of these residues without proper treatment leads to environmental pollution and negative health effects. The recent advances in valorization of agro-industrial wastes by the production of lignocellulolytic enzymes under solid-state fermentation (SSF) are reviewed. SSF is a promising technology to produce lignocellulolytic enzymes. However, the large-scale feasibility is the main challenge of SSF being the control of operational parameters and adequate reactor design the first locks. The current and future trends of SSF bioreactors for lignocellulolytic enzyme production are summarized. SSF allows the production of lignocellulolytic enzymes with high stability at different temperatures and pH, improving their applicability in different industrial settings.This work was supported by the project ‘Development of innovative sustainable protein and omega-3 rich feedstuffs for aquafeeds, from local agroindustrial by-products,’ reference POCI-01-0145-FEDER-030377, funded by European Regional Development Fund (ERDF). JMS was supported by contract from this project. This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. PL was supported by the PhD grant (SFRH/BD/114777/2016) funded by FCT. DS was supported by the doctoral programme ‘Agricultural Production Chains e from fork to farm’ (PD/ 00122/2012). HF was supported by the PhD grant SFRH/BD/131219/2017, and MF was funded by SFRH/BD/143614/2019, both funded by FCT. MG and DF were supported by project InovFeed (ref. MAR-02.01.01-FEAMP0111; Mar2020).info:eu-repo/semantics/publishedVersio