Viabilitas Sel Bakteri Dengan Cryoprotectant Agents Berbeda (Sebagai Acuan Dalam Preservasi Culture Collection di Laboratorium Mikrobiologi)

The aim of this research was to determine the viability of bacterial cells on different cryoprotectant agents. Three kind of cryoprotectant agents and two types of mixed cryprotectant were applied to evaluate the ability of bacteria to growth on Agar medium. Four isolates were used namely Escherichia coli ATCC 35218, Staphylococcus aureus ATCC 25923, Shigella flexneri ATCC 12022 and Bacillus subtilis ATCC 6051. All strains were preserved in 10% skimmed milk, 10% glycerol, 10% DMSO, 10% glycerol:10% skimmed milk and 10% DMSO:10% skimmed milk at -80°C for sixty days. Immediately after preserved, the bacteria were tested their viability at day 30 and 60 during storage. The enumeration of samples taken from cultures in NA media. Better survival of bacterial cells after freezing was shown in all tested cryprotectant agents. Highest microbial viability was shown in 10% skimmed milk, 10% glycerol and mixed 10%DMSO:10% skimmed milk. Hence, those three types of cryoprotectant agents can be recommended to preserve bacterial cell for long term storage

EVALUASI PENERAPAN PRINSIP KESEHATAN DAN KESELAMATAN KERJA (K3) PADA PELAKSANAAN KEGIATAN PRAKTIKUM MIKROTEKNIK DI LABORATORIUM BIOLOGI FAKULTAS SAINS DAN TEKNOLOGI UIN SUNAN KALIJAGA

The aim of this research was to determine the implementation of the principles of health and safety in Microtechnic Laboratory Practice. This research was a qualitative study with descriptive quantitative data analysis. The data were obtained using several instruments: questionnaires, field observation and interview sheets to the lecturers and laboratory assistants. The application of the principles of health and safety, especially in using personal protective equipments is not maximized implemented. Found some students did not wear the personal protective equipments while Microtechnic laboratory practice lasted. The students awareness of the importance of health and safety in the work place needs special attention from the laboratory practices manager for safety and comfort during laboratory activities

Public appraisal of government efforts and participation intent in medico-ethical policymaking in Japan: a large scale national survey concerning brain death and organ transplant

BACKGROUND: Public satisfaction with policy process influences the legitimacy and acceptance of policies, and conditions the future political process, especially when contending ethical value judgments are involved. On the other hand, public involvement is required if effective policy is to be developed and accepted. METHODS: Using the data from a large-scale national opinion survey, this study evaluates public appraisal of past government efforts to legalize organ transplant from brain-dead bodies in Japan, and examines the public's intent to participate in future policy. RESULTS: A relatively large percentage of people became aware of the issue when government actions were initiated, and many increasingly formed their own opinions on the policy in question. However, a significant number (43.3%) remained unaware of any legislative efforts, and only 26.3% of those who were aware provided positive appraisals of the policymaking process. Furthermore, a majority of respondents (61.8%) indicated unwillingness to participate in future policy discussions of bioethical issues. Multivariate analysis revealed the following factors are associated with positive appraisals of policy development: greater age; earlier opinion formation; and familiarity with donor cards. Factors associated with likelihood of future participation in policy discussion include younger age, earlier attention to the issue, and knowledge of past government efforts. Those unwilling to participate cited as their reasons that experts are more knowledgeable and that the issues are too complex. CONCLUSIONS: Results of an opinion survey in Japan were presented, and a set of factors statistically associated with them were discussed. Further efforts to improve policy making process on bioethical issues are desirable

PENGARUH MINAT PEMANFAATAN SISTEM INFORMASI DAN PENGGUNAAN SISTEM INFORMASI TERHADAP KINERJA INDIVIDU (Studi Pada PT. SAMATOR GAS INDUSTRI)

Technological developments from the impact of globalization is so rapid now accompanied with the development of technology-based information systems. Along with the development of information technology, every organization now considers that the information system is essential to the survival of the company. Information Systems can be defined as a set of formal procedures in which the data are collected, processed into information, and distributed to users. Development of Information Systems are intimately associated with the operational accounting useful for every organization. The purpose of this study was to obtain empirical evidence of the influence of performance expectations, expectations of business, social influences on interest in the utilization of partial information systems, to obtain empirical evidence of the influence of interest in the utilization of information systems against the use of information systems, to obtain empirical evidence of the influence of the use of Information Systems on the performance of individual . The population in this study were all employees of PT. Samator Gas Industry, while 47 respondents sample taken with the sampling technique of purposive sampling. Data used primary data using questionnaire data collection method. In this research, there are five hypotheses that have been processed using the Partial Least System. Consisting of three hypotheses received namely, the existence of a positive relationship between Performance Expectancy with Intention of Information System, Intention of Information System with Information System Usage, Information System Usage with Individual Performance. While the two hypotheses are rejected namely, the absence of a positive relationship between Effort Expectancy with Intention of Information System , Social Influence with Intention of Information System

Penyimpangan Hak Kewalian Pernikahan: Studi kasus di Kantor Urusan Agama Kec. Lowokwaru Malang

ABSTRACT Marrage is a contract that permit association and unit righ, and obligation, and helping between a man an awomanthat both of them are not forbidden, A manage can be called as legal when it has full filed requirement and basic principles that have been determined in religion. And law of state, such as in constituition No. 1 year 1974 chaper 2 text 1. The reqwirementsand the bassic principles in islam they are: 1. future husband and future wife, both of them are moslems, 2. contract (sighat) 3. a guardian (male relative of bride, 4. two witnesses. KHI as the constitution of moslem that folow Syafi’i trend use guardian as part of the requirements of marrage. The are sequences to defermine the men have righ to be guardians. The farians of guardins can be devided nto family guardians and a judge guardian (if the are no the first). Besed and Islamic law, if there are no family guardians or if they refuse to be, so a judge guardian replace position of family guardians. But, in KA Lowokwaru distric there is a case that unsuitable with Islamic law. It was a step father could tie a guardian while family guardian was still alive. The aim of this research is to know factors that raise step fother as one of the guardians in KA Lowokwaru district Malang and the reasons why the institution allow, step father to be a guardian to search the problem, the seaches use description anlyze methode with quantity approach. The searches limit the problem of guardian in merrage. The result of searching shows that the reasons raise step father as guardian are 1. own father doesn’t give love to his daughteer, 2. own father never give expenses to his daughter since her bith, 3. there is an agreement from own father and there is desire from the bride, 4. the lack of transparation on part of person conemedto KUA and and the lack of accuracy from KUA

„Die Metaphysik der evolutionären Ökonomik“, in: Erwägen, Wissen, Ethik (EWE) vormals Ethik und Sozialwissenschaften (EuS): Streitforum für Erwägungs- kultur

Köllmann C. Die Metaphysik der evolutionären Ökonomik. In: Erwägen, Wissen, Ethik(EWE) vormals Ethik und Sozialwissenschaften (EuS) ., ed. Streitforum fürErwägungskultur. Vol 15. 2004: 80-82