Quantification of adenovirus serotype B3 in supernatant or pellet of macrophage THP-1 (A) or <i>A</i>. <i>castellanii</i> (B).

<p>All values correspond to mean of six replicates. Values with stars are significantly different (p < 0.05 for * and p < 0.01 for ** Mann-Whitney test). Similar results were obtained after incubation of the co-culture <i>A</i>. <i>castellanii</i> / HAdV B3 at 37°C.</p

Transmission electron microscopy images of (A, B) macrophages THP-1 and (C) <i>A</i>. <i>castellanii</i> trophozoïtes infected by adenovirus serotype B3 after 72 h of co-culture adenovirus elements are indicated by arrows.

<p>Scalebar 1 μm A, C and 0,2 μm B.</p

Immunofluorescence results (A-E) macrophages THP-1, (F-J) <i>A</i>. <i>castellanii</i>, (A,F) mock-infected, (B,G) 1h, (C,H)24h, (D,I) 48h, and (E,J) 72h post-infection.

<p>Adenovirus antigens are stained in green in phagocytics cells counterstained in red.</p