25 research outputs found
Changes in the Polyphenolic Profile and Antioxidant Activity of Wheat Bread after Incorporating Quinoa Flour
This research was funded by grant PID2019-107650RB-C21 funded by MCIN/AEI/ 10.13039/501100011033 (Agencia Estatal de Investigacion del Ministerio de Ciencia e Innovacion, Spain), and by grant 119RT0S67 funded by CYTED (Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo).Quinoa is a trend and a promising functional food ingredient. Following previous research
into the impact of incorporating quinoa flour on the polyphenol content and antioxidant activity of
bread, this study aimed to bridge an existing gap about the qualitative and quantitative polyphenolic
profiles of such bread. The UPLC-MS/MS analysis showed that quinoa bread, made with 25%
quinoa flour of a black variety, presented more compounds than refined-wheat bread, and levels
were remarkably higher in many cases. Consequently, the quinoa bread presented clearly improved
polyphenolic content than the wheat bread (12.8-fold higher considering the sum of extractable
and hydrolyzable polyphenols), as supported by greater antioxidant activity (around 3-fold). The
predominant compounds in the extractable fraction of quinoa bread were p-hydroxybenzoic acid
and quercetin (50- and 64-fold higher than in wheat bread, respectively) and rutin (not detected in
wheat bread), while ferulic and sinapic acids were the most abundant compounds in the hydrolyzable
fraction (7.6- and 13-fold higher than in wheat bread, respectively). The bread-making impact was
estimated, and a different behavior for phenolic acids and flavonoids was observed. Extractable
phenolic acids were the compounds that decreased the most; only 2 of 12 compounds were enhanced
(p-hydroxybenozoic and rosmarinic acid with increments of 64% and 435%, respectively). Flavonoids
were generally less affected, and their concentrations considerably rose after the bread-making
process (7 of the 13 compounds were enhanced in the extractable fraction) with especially noticeably
increases in some cases; e.g., apigenin (876%), kaempferol (1304%), luteolin (580%) and quercetin
(4762%). Increments in some extractable flavonoids might be explained as a consequence of the
release of the corresponding hydrolyzable forms. The present study provides new information on the
suitability of quinoa-containing bread as a suitable vehicle to enhance polyphenols intake and, hence,
the antioxidant activity in daily diets.Agencia Estatal de Investigacion del Ministerio de Ciencia e Innovacion, Spain PID2019-107650RB-C21
MCIN/AEI/ 10.13039/501100011033CYTED (Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo) 119RT0S6
An Olive-Derived Extract 20% Rich in Hydroxytyrosol Prevents beta-Amyloid Aggregation and Oxidative Stress, Two Features of Alzheimer Disease, via SKN-1/NRF2 and HSP-16.2 in Caenorhabditis elegans
The authors gratefully acknowledge the funding support of FEDER/Junta de AndaluciaConsejeria de Economia y Conocimiento, Grant B-AGR-193-UGR18. Also grant PID2019-106778RBI00, funded by MCIN/AEI/10.13039/501100011033 FEDER "Una manera de hacer Europa".Olive milling produces olive oil and different by-products, all of them very rich in different bioactive compounds like the phenolic alcohol hydroxytyrosol. The aim of the present study was to investigate the effects of an olive fruit extract 20% rich in hydroxytyrosol on the molecular mechanisms associated with Alzheimer disease features like A beta- and tau- induced toxicity, as well as on oxidative stress in Caenorhabditis elegans. Moreover, characterization of the extracts, regarding the profile and content of phenolics, as well as total antioxidant ability, was investigated. The study of lethality, growth, pharyngeal pumping, and longevity in vivo demonstrated the lack of toxicity of the extract. One hundred mu g/mL of extract treatment revealed prevention of oxidative stress and a delay in A beta-induced paralysis related with a lower presence of A beta aggregates. Indeed, the extract showed the ability to avoid a certain degree of proteotoxicity associated with aggregation of the tau protein. According to RNAi tests, SKN-1/NRF2 transcription factor and the overexpression of HSP-16.2 were mechanistically associated in the observed effects.FEDER/Junta de AndaluciaConsejeria de Economia y Conocimiento B-AGR-193-UGR18
PID2019-106778RBI00
MCIN/AEI/10.13039/50110001103
A Comparison of Changes in the Fatty Acid Profile of Human Milk of Spanish Lactating Women during the First Month of Lactation Using Gas Chromatography-Mass Spectrometry. A Comparison with Infant Formulas
Breastfeeding is the ideal way to provide infants with the nutrients they need for healthy
growth and development. Milk composition changes throughout lactation, and fat is one of the
most variable nutrients in human milk. The aim of this study was to determine the main differences
between the fatty acid (FA) profile of human milk samples (colostrum, transitional, and mature
milk group) and infant formulas. Human milk samples were provided by lactating women from
Granada. Moreover, different commercial infant formulas were analyzed. FAs were determined using
gas chromatography coupled with mass spectrometry. According to the results, oleic acid was the
predominant monounsaturated fatty acid (41.93% in human milk and 43.53% in infant formulas),
while palmitic acid was the most representative saturated fatty acid (20.88% in human milk and
23.09% in infant formulas). Significant differences were found between human milk groups and infant
formulas, mainly in long-chain polyunsaturated FAs (LC-PUFAs). The content of araquidonic acid
(AA) and docoxahexaenoic acid (DHA) was higher in human milk (0.51% and 0.39%, respectively)
than in infant formulas (0.31% and 0.22%, respectively). Linoleic acid (LA) percentage (15.31%) in
infant formulas was similar to that found in human milk (14.6%). However, alpha-linolenic acid (ALA)
values were also much higher in infant formulas than in human milk (1.64% and 0.42%, respectively).This project was supported by the Spanish Ministry of Science, Innovation and Universities (Project
FIS-ISCIII PI17/02305) and the AGR-279 research group of the Department of Nutrition and Bromatology (University
of Granada)
Amyloid β-but not Tau-induced neurotoxicity is suppressed by Manuka honey via HSP-16.2 and SKN-1/Nrf2 pathways in an in vivo model of Alzheimer’s disease
Alzheimer’s is a chronic degenerative disease of the central nervous system considered the leading cause
of dementia in the world. It is characterized by two etiopathological events related to oxidative stress: the
aggregation of β-amyloid peptide and the formation of neurofibrillary tangles of hyperphosphorylated
Tau protein in the brain. The incidence of this disease increases with age and has been associated with
inadequate lifestyles. Some natural compounds have been shown to improve the hallmarks of the disease.
However, despite its potential, there is no scientific evidence about Manuka honey (MH) in this regard. In
the present work we evaluated the effect of MH on the toxicity induced by Aβ aggregation and Tau in a
Caenorhabditis elegans model. Our results demonstrated that MH was able to improve indicators of oxidative
stress and delayed Aβ-induced paralysis in the AD model CL4176 through HSP-16.2 and SKN-1/
NRF2 pathways. Nevertheless, its sugar content impaired the indicators of locomotion (an indicator of tau
neurotoxicity) in both the transgenic strain BR5706 and in the wild-type N2 worms.MCIN/AEI FPU2017/04358FSE "El FSE invierte en tu futuro" FPU2018/05301JdC-I post-doctoral contract - NextGenerationEU IJC2020-043910-IFEDER/Junta de Andalucia-Consejeria de Economia y Conocimiento B-AGR-193-UGR1
Aproximaciones analíticas –in vitro e –in vivo para evaluar la liberación del aroma en condiciones de consumo de vino
Por su importancia en el aroma de los vinos, una gran mayoría de trabajos científicos se ha centrado primero en identificar los compuestos que integran la fracción volátil del vino empleando técnicas basadas en la cromatografía de gases (GC-FID, GC-MS), y en segundo lugar, en conocer su significado sensorial mediante el empleo de técnicas olfatoméricas (GC-O). Sin embargo, durante el
consumo de alimentos, los compuestos del aroma se liberan en el interior de la cavidad oral y faríngea, pudiendo estar expuestos a diferentes transformaciones químicas y bioquímicas debidas a factores relacionados con la fisiología oral (enzimas de la saliva, temperatura, cambios de pH, adsorción a las mucosas orales, etc). Todos estos factores pueden modificar la composición del aroma original del vino y condicionar la cantidad y tipo de compuestos que finalmente interaccionarán con los órganos olfativos. Entender cómo influyen todos estos procesos requiere de una metodología analítica específicamente diseñada para monitorizar el aroma liberado en el propio individuo durante el consumo (métodos in vivo), o bien el desarrollo de dispositivos que mimeticen los procesos fisiológicos que tienen lugar durante la ingestión del vino (métodos in vitro). Todas estas aproximaciones analíticas y algunas de las aplicaciones en las que se han empleado en el caso del vino serán revisadas en este artículo.Peer reviewe
Differences in the Phenolic Profile by UPLC Coupled to High Resolution Mass Spectrometry and Antioxidant Capacity of Two Diospyros kaki Varieties
Background: phenolic compounds are bioactive chemical species derived from fruits and
vegetables, with a plethora of healthy properties. In recent years, there has been a growing interest in persimmon (Diospyros kaki L.f.) due to the presence of many different classes of phenolic
compounds. However, the analysis of individual phenolic compounds is difficult due to matrix
interferences. Methods: the aim of this research was the evaluation of individual phenolic compounds
and antioxidant capacity of the pulp of two varieties of persimmon (Rojo Brillante and Triumph) by
an improved extraction procedure together with a UPLC-Q-TOF-MS platform. Results: the phenolic
compounds composition of persimmon was characterized by the presence of hydroxybenzoic and hydroxycinnamic acids, hydroxybenzaldehydes, dihydrochalcones, tyrosols, flavanols, flavanones, and
flavonols. A total of 31 compounds were identified and 17 compounds were quantified. Gallic acid was
the predominant phenolic compounds found in the Rojo Brillante variety (0.953 mg/100 g) whereas the
concentration of p-hydroxybenzoic acid was higher in the Triumph option (0.119 mg/100 g). Conclusions: the results showed that the Rojo Brillante variety had higher quantities of phenolic compounds
than the Triumph example. These data could be used as reference in future phenolic compound
databases when individual health effects of phenolic compounds become available.Spanish Ministry of Economy and Competitiveness
AGL2014-53895-REuropean Union (EU
Interactions among odorants, phenolic compounds, and oral components and their effects on wine aroma volatility
This article belongs to the Special Issue Food Oral Processing and Flavour.To determine the impact of oral physiology on the volatility of typical wine aroma compounds, mixtures of a synthetic wine with oral components (centrifuged human saliva (HS), artificial saliva with mucin (AS), and buccal epithelial cells (BC)) were prepared. Each wine type was independently spiked with four relevant wine odorants (guaiacol, β-phenyl ethanol, ethyl hexanoate, and β-ionone). Additionally, the impact of four types of phenolic compounds (gallic acid, catechin, grape seed extract, and a red wine extract) on aroma volatility in the HS, AS, and BC wines was also assessed. Static headspace was measured at equilibrium by solid phase microextraction–GC/MS analysis. Results showed a significant impact of oral components on the volatility of the four tested odorants. Independently of the type of aroma compound, aroma volatility was in general, higher in wines with BC. Moreover, while guaiacol and ethyl hexanoate volatility was significantly lower in wines with HS compared to wines with AS, β-ionone showed the opposite behavior, which might be related to metabolism and retention of mucin, respectively. Phenolic compounds also showed a different effect on aroma volatility depending on the type of compound and wine. Gallic acid had little effect on polar compounds but it enhanced the volatility of the most hydrophobic ones (ethyl hexanoate and β-ionone). In general, flavonoid type polyphenols significantly reduced the volatility of both polar (guaiacol and β-phenyl ethanol) and hydrophobic compounds (β-ionone in HS and BC wines), but through different mechanisms (e.g., π–π interactions and hydrophobic binding for polar and apolar odorants respectively). On the contrary, flavonoids enhanced the volatility of ethyl hexanoate, which might be due to the inhibition exerted on some salivary enzymes (e.g., carboxyl esterase) involved in the metabolism of this odorant molecule.This work was funded by the Spanish MINECO through the AGL201678936-R (AEI/FEDER, UE) Project. M.P-J and C.M.-G. thank the FPI and JdC programs (MINECO) for their PhD and postdoctoral contracts respectively
Aroma release in the oral cavity after wine intake is influenced by wine matrix composition
International audienceThe aim of this study has been to investigate if wine matrix composition might influence the interaction between odorants and oral mucosa in the oral cavity during a "wine intake-like" situation. Aroma released after exposing the oral cavity of three individuals to different wines (n = 12) previously spiked with six target aromas was followed by an -in vivo intra-oral SPME approach. Results showed a significant effect of wine matrix composition on the intra-oral aroma release of certain odorants. Among the wine matrix parameters, phenolic compounds showed the largest impact. This effect was dependent on their chemical structure. Some phenolic acids (e.g. hippuric, caffeic) were associated to an increase in the intra-oral release of certain odorants (e. g. linalool, beta-ionone), while flavonoids showed the opposite effect, decreasing the intra-oral release of aliphatic esters (ethyl hexanoate). This work shows for the first time, the impact of wine composition on oral-mucosa interactions under physiological conditions
Strawberry (Fragaria × ananassa cv. Romina) methanolic extract attenuates Alzheimer’s beta amyloid production and oxidative stress by SKN-1/NRF and DAF-16/FOXO mediated mechanisms in C. elegans
Maria D. Navarro-Hortal and Jose M. Romero-Marquez are FPU fellows from the Spanish Ministry of Educacion y Formacion Profesional. Funding for open access charge: Universidad de Granada/CBUA.Bioactive compounds from strawberries have been associated with multiple healthy benefits. The present study
aimed to assess chemical characterization of a methanolic extract of the Romina strawberry variety in terms of
antioxidant capacity, polyphenols profile and chemical elements content. Additionally, potential toxicity, the
effect on amyloid-β production and oxidative stress of the extract was in vivo evaluated in the experimental model
Caenorhabditis elegans. Results revealed an important content in phenolic compounds (mainly ellagic acid and
pelargonidin-3-glucoside) and minerals (K, Mg, P and Ca). The treatment with 100, 500 or 1000 μg/mL of
strawberry extract did not show toxicity. On the contrary, the extract was able to delay amyloid β-protein
induced paralysis, reduced amyloid-β aggregation and prevented oxidative stress. The potential molecular
mechanisms present behind the observed results explored by RNAi technology revealed that DAF-16/FOXO and
SKN-1/NRF2 signaling pathways were, at least partially, involved.Universidad de Granada/CBU
Intra-oral adsorption and release of aroma compounds following in-mouth wine exposure
Wine >after-odour> defined as the long lasting aroma perception that remains after wine swallowing is an outstanding characteristic in terms of wine quality but a relatively unstudied phenomenon. Among the different parameters that might affect wine after-odour, the adsorption of odorants by the oral mucosa could be important but has been little explored. In this work, the impact of the chemical characteristics of aroma compounds on intra-oral adsorption was assessed by an in vivo approach that determined the amounts of odorants remaining in expectorated wine samples. In addition, the subsequent aroma release after in-mouth wine exposure was studied by means of intra-oral SPME/GC-MS using three different panellists. Oral adsorption of the aroma compounds added to the wines ranged from 6% to 43%, depending on their physicochemical characteristics. A progressive intra-oral aroma decrease at different decay rates depending on compound type and panellist was also found. The strength of the aroma-oral mucosa interactions seems to explain these results more than the amount of compound adsorbed by the oral mucosa.The study was supported by project AGL2012-40172-C02-01 from the Spanish Ministry of Economy and Competitiveness (MINECO). A.E.-F thanks the Spanish MINECO and C.M.-G. thanks the European Social Fund and Jae-Predoc program (CSIC) for their respective research contracts.Peer Reviewe