Bcl-2 proteins and mitochondria—Specificity in membrane targeting for death

AbstractThe localization and control of Bcl-2 proteins on mitochondria is essential for the intrinsic pathway of apoptosis. Anti-apoptotic Bcl-2 proteins reside on the outer mitochondrial membrane (OMM) and prevent apoptosis by inhibiting the activation of the pro-apoptotic family members Bax and Bak. The Bcl-2 subfamily of BH3-only proteins can either inhibit the anti-apoptotic proteins or directly activate Bax or Bak. How these proteins interact with each other, the mitochondrial surface and within the OMM are complex processes we are only beginning to understand. However, these interactions are fundamental for the transduction of apoptotic signals to mitochondria and the subsequent release of caspase activating factors into the cytosol. In this review we will discuss our knowledge of how Bcl-2 proteins are directed to mitochondria in the first place, a crucial but poorly understood aspect of their regulation. This article is part of a Special Issue entitled Mitochondria: the deadly organelle

Late Holocene onset of intensive cultivation and introduction of the falaj irrigation system in the Salut oasis (Sultanate of Oman)

This paper discusses the time and steps of the introduction of intensive agriculture and evolution of irrigation systems to sustain crops in the palaeo-oasis of Salut in the northern Sultanate of Oman. Various geoarchaeological methods allow reconstructing the exploitation of the natural resources of the region and technological development of irrigation methods since the Mid-Holocene. Intensive agriculture started during the Bronze Age and continued with some spatial and intensity fluctuations up to the Islamic period. Cultivations were initially sustained by surface irrigation systems and later replaced by a dense net of aflaj, the typical surface/underground system adopted in the Levant, Arabian Peninsula and western Asia to collect water from deep piedmont aquifers and redistribute it to the fields located in the lowlands. Our results indicate that the aflaj were in use for a long period in the palaeo-oasis formed along Wadi Sayfam and surrounding the citadel of Salut. Uranium-Thorium dating of calcareous tufa formed in the underground tunnels of the aflaj suggests that they were used between ∼540 BCE and ∼1150 CE. After ∼1150 CE Wadi Sayfam were abandoned and the size of the oasis shrank substantially. During the late Islamic period, a surface aqueduct descending from the piedmont of Jabal Shams secured water supply. Our work confirms that in arid lands archaeological and historical communities were able to actively modulate their response to climate changes by using a variety of technological strategies

Alpha proteobacterial ancestry of the [Fe-Fe]-hydrogenases in anaerobic eukaryotes

Eukaryogenesis, a major transition in evolution of life, originated from the symbiogenic fusion of an archaea with a metabolically versatile bacterium. By general consensus, the latter organism belonged to α proteobacteria, subsequently evolving into the mitochondrial organelle of our cells. The consensus is based upon genetic and metabolic similarities between mitochondria and aerobic α proteobacteria but fails to explain the origin of several enzymes found in the mitochondria-derived organelles of anaerobic eukaryotes such as Trichomonas and Entamoeba. These enzymes are thought to derive from bacterial lineages other than α proteobacteria, e.g., Clostridium - an obligate anaerobe. [FeFe]-hydrogenase constitues the characteristic enzyme of this anaerobic metabolism and is present in different types also in Entamoeba and other anaerobic eukaryotes. Here we show that α proteobacteria derived from metagenomic studies possess both the cytosolic and organellar type of [FeFe]-hydrogenase, as well as all the proteins required for hydrogenase maturation. These organisms are related to cultivated members of the Rhodospirillales order previously suggested to be close relatives of mitochondrial ancestors. For the first time, our evidence supports an α proteobacterial ancestry for both the anaerobic and the aerobic metabolism of eukaryotes. Reviewers: This article was reviewed by William Martin and Nick Lane, both suggested by the Authors. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13062-016-0136-3) contains supplementary material, which is available to authorized users

Challenge 8: Digital Citizenship

Digital relations are deeply transforming our lives : from the nature of political participation to the relationship between digital and non-digital environments ; from the reorganization of the public sphere to the ethics of responsibility, transparency or inclusiveness. We are witnessing fundamental changes in the infrastructures of democracy and the emergence of new forms of digital citizenship.Peer reviewe