A Qualitative Examination of Surviving Homeless in Alaska

The homeless population in Anchorage, Alaska faces many unique challenges. Over the past several winters, a number of homeless individuals have succumbed to the effects of exposure despite available cold weather services. This study investigated individual experiences within the homeless population of Anchorage, Alaska during times of inclement winter weather. Self-determination theory was used to explore motivations of behaviors of the population and to uncover the reasons why this population does not use cold weather services offered by the Municipality of Anchorage. The research questions addressed participant awareness of available cold weather services, survival strategies during inclement winter weather, and barriers to cold weather service use. This phenomenological study examined those lived experiences. Purposeful sampling was used to recruit eligible individuals (n = 14) at a local soup kitchen, and participants engaged in semistructured interviews. Data analysis procedures used McCormack\u27s lenses and horizontalization to reveal emerging themes. Key findings included a lack of knowledge of emergency services, various survival strategies, and self-imposed barriers to services. The implications of these emerging revelations may positively influence public health providers to modify education delivery methods and interventions used to reach the homeless population in Anchorage, Alaska, with the ultimate goal of preventing wintertime mortalities

Kaposi’s sarcoma-associated herpesvirus (KSHV) LANA prevents KSHV episomes from degradation

Protein knockdown with an inducible degradation system is a powerful tool for studying proteins of interest in living cells. Here, we adopted the auxin-inducible degron (AID) approach to detail Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) function in latency maintenance and inducible viral lytic gene expression. We fused the mini-auxin-inducible degron (mAID) tag at the LANA N-terminus with KSHV bacterial artificial chromosome 16 recombination, and iSLK cells were stably infected with the recombinant KSHV encoding mAID-LANA. Incubation with 5-phenyl-indole-3-acetic acid, a derivative of natural auxin, rapidly degraded LANA within 1.5 h. In contrast to our hypothesis, depletion of LANA alone did not trigger lytic reactivation but rather decreased inducible lytic gene expression when we stimulated reactivation with a combination of ORF50 protein expression and sodium butyrate. Decreased overall lytic gene induction seemed to be associated with a rapid loss of KSHV genomes in the absence of LANA. The rapid loss of viral genomic DNA was blocked by a lysosomal inhibitor, chloroquine. Furthermore, siRNA-mediated knockdown of cellular innate immune proteins, cyclic AMP-GMP synthase (cGAS) and simulator of interferon genes (STING), and other autophagy-related genes rescued the degradation of viral genomic DNA upon LANA depletion. Reduction of the viral genome was not observed in 293FT cells that lack the expression of cGAS. These results suggest that LANA actively prevents viral genomic DNA from sensing by cGAS-STING signaling axis, adding novel insights into the role of LANA in latent genome maintenance.IMPORTANCESensing of pathogens' components is a fundamental cellular immune response. Pathogens have therefore evolved strategies to evade such cellular immune responses. KSHV LANA is a multifunctional protein and plays an essential role in maintaining the latent infection by tethering viral genomic DNA to the host chromosome. We adopted the inducible protein knockdown approach and found that depletion of LANA induced rapid degradation of viral genomic DNA, which is mediated by innate immune DNA sensors and autophagy pathway. These observations suggest that LANA may play a role in hiding KSHV episome from innate immune DNA sensors. Our study thus provides new insights into the role of LANA in latency maintenance

Relationship between parenting styles and emotional stability of senior high school students of De La Salle Health Sciences Institute

This study used descriptive correlational, non-experimental design. Purposive and convenience sampling were used to select 200 respondents. The research questionnaire used were adapted from the parenting style tool used by Anies, Martinez, Rapal and Soriano (2007) entitled The relationship between perceived parenting styles and emotional intelligence among BSN students of Batch 2009 in De La Salle Health Sciences Campus, which was guided by Diana Baumrind\u27s theory on parenting styles. Data was analyzed through frequency distribution, percentage distribution, mean, standard deviation and chi-square. The study found that, 1) Most senior high school students of the De La Salle Health Sciences Institute Batch 2017-2018 who were raised by their parent/s were 17-18 years old, male, Grade 12, youngest child, had an employed mother and father and belonged to a nuclear family; 2) Majority of the respondents\u27 parents used the authoritative parenting style for child rearing; 3) There was a normal level of emotional stability when responding to academic, interpersonal and family related stressors; 4) Age, gender, grade level, ordinal position, parent/s employment status, and family structure are not associated with the parenting style of the parent’s respondents except for religion; 5) Age, gender, grade level, ordinal position, parent/s employment status, and family structure and religion are not associated with the emotional stability of the respondents; 6) There was no significant relationship between the parenting style and emotional stability of the respondents

An FPGA-in-the-Loop Simulation of a Neural Network-based Optimization of Greenhouse Supplemental Illumination

—Greenhouse crops depend on sufficient amount of light to achieve photosynthetic efficiency resulting to their full development and growth. So supplemental illumination is needed when insufficient light has accumulated throughout the day to help achieve the required Daily Light Integral (DLI) for the crops. The lighting schedule and the energy usage are the top concerns of the crop growers in their supplemental lighting operations. In this study, an FPGA-based system was developed to manage the operations of the supplemental illumination, giving the crop growers the option to set the lighting schedule and the target DLI supplementation by selecting the desired energy saving mode. The designed system was able to control the lighting operation within the lighting schedule set by the user. Its capability of saving energy is dependent on the daytime lighting. In testing, the system was able to save energy when high to average energy saving mode selected for low to average target DLI, but not when selecting low energy saving mode for high target DLI supplementation. The system was capable of achieving the target DLI value based on the chosen target DLI requirement for the crops

Obstructive sleep apnea: Underestimated risk factor in sudden cardiac death in schizophrenia

Fundação de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Univ Fed Sao Paulo, Disciplina Neurociencia, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Psicobiol, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilUniv Fed Sao Paulo, Disciplina Neurociencia, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Psicobiol, Escola Paulista Med UNIFESP EPM, Sao Paulo, BrazilWeb of Scienc