The pine wood nematode, Bursaphelenchus xylophilus, in Portugal: possible introductions and spread routes of a serious biological invasion revealed by molecular methods

The pine wood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (PWD), is a major world-wide pathogen and pest of pine, with impacts on forest health, natural ecosystem stability and international trade. In Portugal, PWN was first diagnosed in 1999, the first occurrence also for Europe. The disease was recently detected on the island ofMadeira and in northern Spain. In an attempt to search for more reliable and robust molecular markers that enable the study of intraspecific variability of B. xylophilus from different geographic locations, the intergenic spacer (IGS) region of the 5S rRNA gene and inter-simple sequence repeats (ISSR) analysis were used to determine the genetic relationships among 43 B. xylophilus isolates from Portugal, China, Japan, South Korea and USA. IGS sequence analysis showed that this region can only be used to establish interspecific relationships, since no differences were detected among Portuguese isolates from different geographic locations. Fingerprints obtained with ISSR show high genetic variability among Portuguese isolates, except for the ones obtained prior to 2008. The ISSR dendrogram suggests the spread of the disease inside continental Portugal and to Madeira. Until 2008, B. xylophilus populations found in continental Portugal showed low genetic diversity, pointing to a single introduction, probably from Asia, whereas recent populations from continental Portugal (2009-2010) and Madeira show high genetic diversity, suggesting multiple introductions from different origins

Plan de negocio: Creasur Eventos S.L

Universidad de Sevilla. Grado en Publicidad y Relaciones Pública

Caso clínico sobre el tratamiento fisioterapéutico de un paciente con Atrofia Multisistémica.

Introducción: La Atrofia Multisistémica es una enfermedad degenerativa, que cursa con una disfunción del sistema nervioso autónomo y del sistema motor. El objetivo de este estudio es proponer un programa de tratamiento fisioterapéutico para un paciente diagnosticado de Atrofia Multisistémica. Presentación del caso: se describe la valoración de la capacidad funcional y las necesidades del paciente, los objetivos y un programa de tratamiento adaptado. Resultados: El presente estudio presenta varias limitaciones, una de ellas es la insuficiencia de evidencia científica encontrada y otra escasez de tiempo para el desarrollo del programa de tratamiento. Aun con estas limitaciones los resultados obtenidos son favorables, de acuerdo con las escalas validadas. Es muy importante la realización de una buena exploración fisioterapéutica objetiva, ya que es la herramienta que nos permite establecer un diagnóstico fisioterapéutico y, a partir de él, un programa de tratamiento que incluya todas las necesidades del paciente, proporcionar un pronóstico funcional y realizar un seguimiento. Conclusión: Considero que el programa de ejercicios propuestos son acordes con las necesidades del paciente y el Concepto Bobath aporta una amplia gama de posibilidades para trabajar con estos pacientes. Se debería ahondar en la investigación Atrofia Multisistémica y en su abordaje fisioterapéutico.Introduction: Multiple System Atrophy is a degenerative desease that causes dysfunction of the autonomic nervous system and motor system. The aim of this study is to propose a program of physiotherapy treatment for a patient diagnosed with multiple system atrophy. Case Presentation: We describe the assessment of functional capacity and patient needs, goals and a treatment program tailored. Results: This study presents several limitations, one of them is found insufficient scientific evidence and a shortage of time for development of a treatment program. Even with these limitations the results are favorable, according to validated scales. It is very important to produce a good scan physiotherapy objective, since it is the tool that allows us to establish a physical therapy diagnosis, and from it, a treatment program that includes all the patient's needs, provide a functional outcome and conduct a monitoring. Conclusion: I believe that the proposed exercise program are consistent with the needs of the patient and the Bobath Concept brings a wide range of possibilities for working with these patients. Should be more research in Multiple System Atrophy and physiotherapy for their treatmentGrado en Fisioterapi

Caso clínico sobre el tratamiento fisioterapéutico de un paciente con Atrofia Multisistémica.

Introducción: La Atrofia Multisistémica es una enfermedad degenerativa, que cursa con una disfunción del sistema nervioso autónomo y del sistema motor. El objetivo de este estudio es proponer un programa de tratamiento fisioterapéutico para un paciente diagnosticado de Atrofia Multisistémica. Presentación del caso: se describe la valoración de la capacidad funcional y las necesidades del paciente, los objetivos y un programa de tratamiento adaptado. Resultados: El presente estudio presenta varias limitaciones, una de ellas es la insuficiencia de evidencia científica encontrada y otra escasez de tiempo para el desarrollo del programa de tratamiento. Aun con estas limitaciones los resultados obtenidos son favorables, de acuerdo con las escalas validadas. Es muy importante la realización de una buena exploración fisioterapéutica objetiva, ya que es la herramienta que nos permite establecer un diagnóstico fisioterapéutico y, a partir de él, un programa de tratamiento que incluya todas las necesidades del paciente, proporcionar un pronóstico funcional y realizar un seguimiento. Conclusión: Considero que el programa de ejercicios propuestos son acordes con las necesidades del paciente y el Concepto Bobath aporta una amplia gama de posibilidades para trabajar con estos pacientes. Se debería ahondar en la investigación Atrofia Multisistémica y en su abordaje fisioterapéutico.Introduction: Multiple System Atrophy is a degenerative desease that causes dysfunction of the autonomic nervous system and motor system. The aim of this study is to propose a program of physiotherapy treatment for a patient diagnosed with multiple system atrophy. Case Presentation: We describe the assessment of functional capacity and patient needs, goals and a treatment program tailored. Results: This study presents several limitations, one of them is found insufficient scientific evidence and a shortage of time for development of a treatment program. Even with these limitations the results are favorable, according to validated scales. It is very important to produce a good scan physiotherapy objective, since it is the tool that allows us to establish a physical therapy diagnosis, and from it, a treatment program that includes all the patient's needs, provide a functional outcome and conduct a monitoring. Conclusion: I believe that the proposed exercise program are consistent with the needs of the patient and the Bobath Concept brings a wide range of possibilities for working with these patients. Should be more research in Multiple System Atrophy and physiotherapy for their treatmentGrado en Fisioterapi

Empirical vs. Susceptibility-Guided Treatment of Helicobacter pylori Infection: A Systematic Review and Meta-Analysis

Background: Treating Helicobacter pylori infection according to antibiotic resistance has been frequently recommended. However, information on its real effectiveness is scarce. Aim: The aim of this study is to perform a meta-analysis comparing empirical vs. susceptibility-guided treatment of H. pylori. Methods: Selection of studies: Studies comparing empirical versus susceptibility-guided treatment were selected. Search strategy: electronic and manual up to August 2021. Data synthesis: by intention-to-treat (random-effects model). Results: Overall, 54 studies were included (6,705 patients in the susceptibility-guided group and 7,895 in the empirical group). H. pylori eradication rate was 86 vs. 76%, respectively (RR: 1.12; 95% CI: 1.08–1.17; I2: 83%). Similar results were found when only RCTs were evaluated (24 studies; RR: 1.16; 95% CI: 1.11–1.22; I2: 71%) and when susceptibility testing was assessed by culture (RR: 1.12; 95% CI: 1.06–1.18) or PCR (RR: 1.14; 95% CI: 1.05–1.23). For first-line treatments (naïve patients; 30 studies), better efficacy results were obtained with the susceptibility-guided strategy (RR: 1.15; 95% CI: 1.11–1.20; I2: 79%). However, for empirical first-line quadruple regimens, in particular (both with and without bismuth, excluding the suboptimal triple therapies), not based on CYP2C19 gene polymorphism, no differences in efficacy were found compared with the susceptibility-guided group (RR: 1.04; 95% CI: 0.99–1.09); this lack of difference was confirmed in RCTs (RR: 1.05; 95% CI: 0.99–1.12). For rescue therapies (13 studies, most 2nd-line), similar results were demonstrated for both strategies, including all studies (RR: 1.09; 95% CI: 0.97–1.22; I2: 82%) and when only RCTs were considered (RR: 1.15; 95% CI: 0.97–1.36). Conclusion: The benefit of susceptibility-guided treatment over empirical treatment of H. pylori infection could not be demonstrated, either in first-line (if the most updated quadruple regimens are prescribed) or in rescue therapie

Perguntas: projetos de criação artística dos alunos de Teatro, ramo de Design de Cena Licenciatura + Mestrado

O projeto PERGUNTAS assenta na convicção de que o ensino artístico é um ensino experimental, que encontra a sua metodologia no regime laboratorial. É um projeto de criação artística dos estudantes de teatro, ramo de Design de Cena Licenciatura + Mestrado.Projecto financiado no âmbito da 6ª edição do concurso de projectos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa IPL/2021/ECCIC_ESTC.info:eu-repo/semantics/publishedVersio

Falso: projectos de criação artística dos alunos de Teatro, ramo de Design de Cena Licenciatura + Mestrado

Inserido no projeto Polifonia e Contraponto - Criação e Reflexão no Espaço CénicoCatálogo do projecto de criação que parte do tema o FALSO e integra alunos do primeiro, segundo e terceiro ano da Licenciatura em Teatro – ramo de Design de Cena e do Mestrado em Teatro – especialização em Design de Cena que, trabalhando em pequenos grupos, com o apoio do conjunto de professores de Design de Cena, criam objectos e/ou intervenções destinadas à exposição pública.info:eu-repo/semantics/publishedVersio

Adolescentes con alteraciones graves de conducta. ¿Cómo se interviene a nivel educativo en los Institutos de Enseñanza Secundaria?

ABSTRACT Most of the research on severe behavioral disorders (SBD) and school conflicts carried out in our country has focused on peer violence and the scenarios in which it occurs. The aims of the present research are to study the ways in which students who show SBD in high schools of Castilla-La Mancha (Spain) are being dealt with and to check if the obtained results comply the basic prevention criteria provided by the State Study on School Coexistence in Secondary Education (Díaz-Aguado, 2010). A transversal descriptive study is carried out. The participants are the population of educational counselors (N=217) of the high school of the region. An ad hoc questionnaire of interjudge reliability is provided with the aim of responding to research question through 6 categories: process of incorporation into the family, student’s knowledge, individual educational response, educational center response, external services and global assessment. The results indicate that there are three key elements to be highlighted: the need for protocols of educational activities and interventions to be carried out in the educational center and with those students, the lack of teacher training and to improve the coordination with Mental Health and the Social Services. The results determine that the interventions that are currently being carried out are insufficient and should be revised in order to be efficient. This way, a more specific response could be given to the needs of the students with SBD to improve their integral development and the school coexistenceRESUMENLa mayoría de las investigaciones sobre alteraciones graves de conducta (AGC) y conflictividad escolar que se han producido en nuestro país se centran en la violencia entre iguales y los escenarios en los que se produce. En este trabajo se estudia cómo se está interviniendo con el alumnado que presenta AGC en los Institutos públicos de Enseñanza Secundaria Obligatoria (IES) de Castilla-La Mancha (España) y se observa si los datos obtenidos siguen los criterios básicos de prevención que aporta el Estudio Estatal sobre la Convivencia Escolar en Educación Secundaria (Díaz-Aguado, 2010). Se lleva a cabo un estudio descriptivo transversal, con la población de orientadores (N=217) de los IES de la región. Se administra un cuestionario elaborado ad hoc, através de una metodología de validación inter-jueces, con el objetivo de dar respuesta a las preguntas de investigación a través de 6 categorías; proceso de incorporación y familia, conocimiento del alumnado, respuesta educativa individual, respuesta educativa del centro, servicios externos y valoración global. Los resultados indican que hay tres elementos clave a destacar: la necesidad de protocolos de las actividades e intervenciones educativas a realizar en el centro educativo y con estos alumnos, la formación del profesorado y la mayor coordinación con Salud Mental y Servicios Sociales. Los datos determinan que la intervención que se desarrolla en el momento actual es insuficiente y debería ser revisada para dar una respuesta más específica a las necesidades del alumnado con AGC para una mejora de su desarrollo integral y de la convivencia escolar.ABSTRACT Most of the research on severe behavioral disorders (SBD) and school conflicts carried out in our country has focused on peer violence and the scenarios in which it occurs. The aims of the present research are to study the ways in which students who show SBD in high schools of Castilla-La Mancha (Spain) are being dealt with and to check if the obtained results comply the basic prevention criteria provided by the State Study on School Coexistence in Secondary Education (Díaz-Aguado, 2010). A transversal descriptive study is carried out. The participants are the population of educational counselors (N=217) of the high school of the region. An ad hoc questionnaire of interjudge reliability is provided with the aim of responding to research question through 6 categories: process of incorporation into the family, student’s knowledge, individual educational response, educational center response, external services and global assessment. The results indicate that there are three key elements to be highlighted: the need for protocols of educational activities and interventions to be carried out in the educational center and with those students, the lack of teacher training and to improve the coordination with Mental Health and the Social Services. The results determine that the interventions that are currently being carried out are insufficient and should be revised in order to be efficient. This way, a more specific response could be given to the needs of the students with SBD to improve their integral development and the school coexistenc

Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe oleracea Mart.) Using an Experimental Design Methodology. Part 3: Microwave-Assisted Extraction

In this work, two methods based on microwave-assisted extraction techniques for the extraction of both anthocyanins and total phenolic compounds from acai have been developed. For that, a full factorial design (Box-Behnken design) has been used to optimize the following four variables: solvent composition (25-75% methanol in water), temperature (50-100 degrees C), pH (2-7), and sample/solvent ratio (0.5 g: 10 mL-0.5 g: 20 mL). The anthocyanins and total phenolic compounds content have been determined by ultra high-pressure liquid chromatography and Folin-Ciocalteu method, respectively. The optimum conditions for the extraction of anthocyanins were 38% MeOH in water, 99.63 degrees C, pH 3.00, at 0.5 g: 10 mL of ratio, while for the extraction of total phenolic compounds they were 74.16% MeOH in water, 99.14 degrees C, pH 5.46, at 0.5 g: 20 mL of ratio. Both methods have shown a high repeatability and intermediate precision with a relative standard deviation lower than 5%. Furthermore, an extraction kinetics study was carried out using extraction periods ranging from 2 min until 25 min. The optimized methods have been applied to acai-containing real samples. The results with such real samples have confirmed that both methods are suitable for a rapid and reliable extraction of anthocyanins and total phenolic compounds

Extraction of anthocyanins and total phenolic compounds from açai (euterpe oleracea mart.) using an experimental design methodology. part 1: Pressurized liquid extraction

Currently, açai is one of the most important fruits present in the world. Several studies have demonstrated its high content in phenolic compounds and anthocyanins. Both of them are responsible of interesting properties of the fruit such as anti-inflammatory, antioxidant or anticancer. In the present study, two optimized pressurized liquid extraction (PLE) methods have been developed for the extraction of anthocyanins and total phenolic compounds from açai. A full factorial design (Box-Behnken design) with six variables (solvent composition (25%-75% methanol-in-water), temperature (50-100°C), pressure (100-200 atm), purge time (30-90 s), pH (2-7) and flushing (50%-150%)) were employed. The percentage of methanol in the extraction solvent was proven to be the most significant variable for the extraction of anthocyanins. In the case of total phenolic compounds, the extraction temperature was the most influential variable. The developed methods showed high precision, with relative standard deviations (RSD) of less than 5%. The applicability of the methods was successfully evaluated in real samples. In conclusion, two rapid and reliable PLE extraction methods to be used for laboratories and industries to determine anthocyanins and total phenolic compounds in açai and its derived products were developed in this work