Determination of allergenic pollens in the atmosphere of Trabzon province by volumetric method

Bu çalışmada, Trabzon ili atmosferinde bulunan polenler 01 Ocak 2016 – 31 Aralık 2016 tarihleri arasında volumetrik yöntemle VPSS 2000 (Lanzoni) cihazı kullanılarak araştırıldı. Bir yıllık çalışma süresince 45 taksona ait toplam 29150 polen m-3 Trabzon atmosferinde kaydedildi. Tespit edilen bu taksonların 24’ünün odunsu bitkilere (%69.78), 21’inin otsu bitkilere (%30.00) ve tanımlanamayan (%0.23) polenlere ait olduğu belirlendi. Çalışılan bölgede yoğun olarak saptanan odunsu taksonlar; Alnus sp. (%23.06), Cupressaceae-Taxaceae (%19.29), Pinaceae (%11.59), Betula sp. (%5.91), Platanus sp. (%3.38), Quercus sp. (%1.67), Ulmus sp. (%1.16) ve Fraxinus sp. (%1.05) olarak saptandı. Otsu bitkiler ise; Poaceae (%13.56), Urticaceae (%7.10), Boraginaceae (%2.12), Rumex sp. (%1.92), Mercurialis sp. (%1.36) ve Artemisia sp. (%1.25) olduğu belirlendi. Çalışılan bölgede polen yoğunluğunun en fazla olduğu aylar Şubat (%38.39), Mart (%11.88) ve Nisan (%15.67) olarak belirlendi.: In this study, airborne pollen grains of Trabzon were investigated using a volumetric trap VPSS 2000 (Lanzoni) from 01st January 2016 to 31st December 2016. During one-year study period, a total of 29150 polen m-3 belonging to 45 taxa were recorded. From identified taxa, 24 belong to arboreal (69.78%) and 21 taxa to non-arboreal (30.00%) and unidentified pollen grains (0.23%). In the investigated area, from arboreal plant taxa Alnus sp. (23.06%), Cupressaceae-Taxaceae (19.29%), Pinaceae (11.59%), Betula sp. (5.91%), Platanus sp. (3.38%), Quercus sp. (1.67%), Ulmus sp. (1.16%), Fraxinus sp. (1.05%) and from non-arboreal plant taxa Poaceae (13.56%), Urticaceae (7.10%), Boraginaceae (2.12%), Rumex sp. (1.92%), Mercurialis sp. (1.36%), and Artemisia sp. (1.25%) were responsible for the greatest amounts of pollen grains during the study period. The maximum pollen concentration of investigated area is recorded in February (38.39%), March (11.88%) and April (15.67%)

The Impact of Obesity and Insulin Resistance on Iron and Red Blood Cell Parameters: A Single Center, Cross-Sectional Study

OBJECTIVE: Obesity and iron deficiency (ID) are the 2 most common nutritional disorders worldwide causing significant public health implications. Obesity is characterized by the presence of low-grade inflammation, which may lead to a number of diseases including insulin resistance (IR) and type 2 diabetes. Increased levels of acute-phase proteins such as C-reactive protein (CRP) have been reported in obesity-related inflammation. The aim of this study was to investigate the impact of obesity/IR on iron and red blood cell related parameters. METHODS: A total of 206 patients and 45 control subjects of normal weight were included in this crosssectional study. Venous blood samples were taken from each patient to measure hemoglobin (Hb), serum iron (Fe), ironbinding capacity (IBC), ferritin, CRP, fasting blood glucose, and fasting insulin. Body mass index (BMI) and waist-to-hip ratio (WHR) were calculated for each patient. IR was determined using the HOMA-IR formula. RESULTS: Subjects were divided into 3 groups according to BMI. There were 152 severely obese (BMI: 42.6+-10.1), 54 mildly obese (BMI: 32.4+-2.1), and 45 normal-weight (BMI: 24.3+-1.3) patients. Hb levels in severely obese patients and normal controls were 12.8+-1.3 g/dL and 13.6+-1.8 g/dL, respectively. We found decreasing Fe levels with increasing weight (14.9+-6.9 μmol/L, 13.6+-6.3 μmol/L, and 10.9+-4.6 μmol/L for normal controls and mildly and severely obese patients, respectively). Hb levels were slightly lower in patients with higher HOMA-IR values (13.1+-1.5 g/dL vs. 13.2+-1.2 g/dL; p=0.36). Serum iron levels were significantly higher in the group with low HOMA-IR values (13.6+-5.9 μmol/L vs. 11.6+-4.9 μmol/L; p=0.008). IBC was found to be similar in both groups (60.2+-11.4 μmol/L vs. 61.9+-10.7 μmol/L; p=0.23). Ferritin was slightly higher in patients with higher HOMA-IR values (156.1+-209.5 pmol/L vs. 145.3+-131.5 pmol/L; p=0.62). CONCLUSION: Elevated BMI and IR are associated with lower Fe and hemoglobin levels. These findings may be explained by the chronic inflammation of obesity and may contribute to obesity-related co-morbidities. People with IR may present with ID without anemia

The Impact of Obesity and Insulin Resistance on Iron and Red Blood Cell Parameters: A Single Center, Cross-Sectional Study

OBJECTIVE: Obesity and iron deficiency (ID) are the 2 most common nutritional disorders worldwide causing significant public health implications. Obesity is characterized by the presence of low-grade inflammation, which may lead to a number of diseases including insulin resistance (IR) and type 2 diabetes. Increased levels of acute-phase proteins such as C-reactive protein (CRP) have been reported in obesity-related inflammation. The aim of this study was to investigate the impact of obesity/IR on iron and red blood cell related parameters. METHODS: A total of 206 patients and 45 control subjects of normal weight were included in this crosssectional study. Venous blood samples were taken from each patient to measure hemoglobin (Hb), serum iron (Fe), ironbinding capacity (IBC), ferritin, CRP, fasting blood glucose, and fasting insulin. Body mass index (BMI) and waist-to-hip ratio (WHR) were calculated for each patient. IR was determined using the HOMA-IR formula. RESULTS: Subjects were divided into 3 groups according to BMI. There were 152 severely obese (BMI: 42.6±10.1), 54 mildly obese (BMI: 32.4±2.1), and 45 normal-weight (BMI: 24.3±1.3) patients. Hb levels in severely obese patients and normal controls were 12.8±1.3 g/dL and 13.6±1.8 g/dL, respectively. We found decreasing Fe levels with increasing weight (14.9±6.9 μmol/L, 13.6±6.3 μmol/L, and 10.9±4.6 μmol/L for normal controls and mildly and severely obese patients, respectively). Hb levels were slightly lower in patients with higher HOMA-IR values (13.1±1.5 g/dL vs. 13.2±1.2 g/dL; p=0.36). Serum iron levels were significantly higher in the group with low HOMA-IR values (13.6±5.9 μmol/L vs. 11.6±4.9 μmol/L; p=0.008). IBC was found to be similar in both groups (60.2±11.4 μmol/L vs. 61.9±10.7 μmol/L; p=0.23). Ferritin was slightly higher in patients with higher HOMA-IR values (156.1±209.5 pmol/L vs. 145.3±131.5 pmol/L; p=0.62). CONCLUSION: Elevated BMI and IR are associated with lower Fe and hemoglobin levels. These findings may be explained by the chronic inflammation of obesity and may contribute to obesity-related co-morbidities. People with IR may present with ID without anemia

Akut Koroner Sendromlu Hastalarda Serum Gama Glutamiltransferaz, Kalsiyum, Fosfor Değerlerinin Erken Mortalite ile İlişkisi

Amaç: GGT, LDL-kolesterolün oksidasyon basamaklarındaki rolünden dolayı proaterojenik bir moleküldür; ayrıca glutatyon metabolizması ile ilişkili olup oksidatif stres için bir biomarkır olarak düşünülebilir. Serum inorganik fosfor düzeyleri de kardiyovasküler olay ve mortalite için bağımsız bir risk faktörü olşarak kabul edilmektedir. Biz de çalışmamızda akut koroner sendromlu hastalarda erken mortalite ile serum GGT, Ca, P değerlerinin ilişkisini araştırdık. Yöntemler: Çalışmamıza akut koroner sendrom tanısıyla Koroner Yoğun Bakım Ünitesine yatırılan kadın, erkek toplam 200 hasta alındı. Hepatobiliyer sistemle ilgili, kronik metabolik kemik hastalığı, paratiroid ve ciddi sistemik hastalığı olmayan, GFR 'si >60 mL/dk olan olgular çalışmaya alındı. Hastaların biyokimyasal verileri ile taburcu olduktan sonraki bir aylık dönemdeki kardiyak kaynaklı mortalitelerinin ilişkisi değerlendirildi. Serum GGT düzeyinin normal referans aralığı erkekler için 12-64 U/L, kadınlarda ise 9-36 U/L, kalsiyum düzeylerinin normal aralığı 8,4-10,2 mg/dL idi ve inorganik fosfor için üst sınır <=4,5 mg/dL olarak belirlendi. Tanımlayıcı istatistiksel metotların yanı sıra student's t test, Mann Whitney u test ve ki-kare testi kullanıldı. Bulgular: Bir aylık sürecin sonunda mortalite izlenen hastalarda (n=23) serum GGT, fosfor ve CaxP değerlerini (32,96 U/L, 3,66 mg/dL, 35,93 mg2 / dL2 ) mortalite izlenmeyen hastalarla karşılaştırdığımızda (n=177; 24,16 U/L, 3,27 mg/dL, 31,57 mg2 /dL2 ; p<0,001, p<0,001, p<0,001, sırasıyla) anlamlı olarak daha yüksek bulduk. Sonuç: Referans aralığındaki serum GGT aktivitesinin, fosfor ve CaxP dü- zeylerinin geleneksel kardiyovasküler hastalık risk faktörlerinden (Diabetes mellitus, hipertansiyon, iskemik kalp hastalığı öyküsü gibi) bağımsız olarak, kısa dönemde de prognostik değer taşıdığını gösterdik. Çalışmamız yüksek riskli populasyonlarda, hastaların risk açısından sınıflandırılmasına yardımcı olmak, agresiv tedavi yaklaşımları geliştirerek gelecekteki istenmeyen kardiyak ölümleri önlemek amacıyla GGT ve fosfor düzeyleri için yeni referans değerleri belirlenmesi gerekliliğini desteklemektedir.Objective: Gamma-glutamyltransferase (GGT) may be considered as a biomarker of &quot;oxidative stress&quot; associated with glutathione metabolism and a possible &quot;proatherogenic&quot; marker because of its indirect relationship with the biochemical steps in the oxidation of low density lipoprotein cholesterol. Serum inorganic phosphorus (P) level is also suggested as an independent risk factor for cardiac events and mortality in the long term. We aimed to observe the relationship of serum GGT, calcium (Ca), and P levels with 1 months' mortality after myocardial infarction. Methods: Our retrospective study included 200 patients (124 men and 76 women) with acute coronary syndrome (ACS) who were admitted to our hospital. We excluded subjects with severe systemic illness, hepatobiliary disease, alcohol consumption, chronic metabolic bone disease, malignancy, parathyroid disease, and patients who had a glomerular filtration rate (GFR) <60 mL/min. Fasting blood samples were taken in the first 24 h of admission to the coronary care unit (CCU). Reference values for GGT (9-36 U/L, for women; 12-64 U/L, for men), Ca (8.4-10.2 mg/dL), and inorganic P (<=4.5 mg/dL) were used. When the serum albumin level was <4.0 g/dL, corrected Ca levels were calculated using the equation [corrected Ca=measured Ca+(0.8&times;(4-serum albumin)]. Statistical analysis was performed using SPSS for Windows 10.0. Descriptive statistical analysis, Student's t-test, Mann-Whitney U-test, and chi-square test were used. Results: At the end of 1 month, we found significantly higher levels of blood GGT, P, and Ca&times;P products in patients who did not survive (n=23; 32.9 U/L, 3.66 mg/dL, 35.93 mg2 /dL2 ) than in survivors (n=177; 24.16 U/L, 3.27 mg/dL, 31.57 mg2 /dL2 ; p<0.001, p<0.001, p<0.001, respectively). Conclusion: Serum GGT, P, and Ca&times;P levels, even in the reference intervals, had a prognostic value in the short-term mortality apart from traditional risk factors such as diabetes mellitus, hypertension, and ischemic heart disease. This study also suggests constituting new reference values for this high risk population in stratifying patient risk and in assessing the intensity of appropriate treatment, with hopes of preventing cardiac deaths

Evaluation of MDRD, cockcroft-gault, and CICD-EPI formulas in the estimated glomerular filtration rate

WOS: 000350097600014PubMed: 25651715Background: The aim of this study was to compare the use of the ideal weight with the use of the patient's actual weight in the C-G (Cockcroft-Gault) formula for the measurement of the GFR (Glomerular Filtration Rate). We also aimed to compare the results of the calculations explained above with the results of the MDRD (Modification of Diet in Renal Disease) formula and CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) results of the classical 24-hour creatinine clearance method. Methods: Creatinine clearance values, which were obtained from 24-hour urine collection, were compared with the values from the C-G formula in which each patient's ideal weight was used, with the values from the C-G formula in which each patient's actual body weight was used, and with the MDRD Formula and CKD-EPI. Results: The correlation analysis between 24-hour creatinine clearance and the GFR obtained from the C-G formula with adjusted ideal weight in the Control group, Group I (patients with diabetes mellitus) and Group II resulted in values of r = 0.526, 0.576, and 0.850 (p < 0.0001), respectively. The correlation analysis between 24-hour creatinine clearance and the MDRD formula among the same groups resulted in r = 0.814, 0.682, and 0.861 (p < 0.0001), respectively. The correlation analysis between creatinine clearance and the CKD-EPI formula among the same groups resulted in r = 0.821, 0.679, and 0.871 (p < 0.0001), respectively. Conclusions: The results of the CKD-EPI formula were the most compatible with the results of 24-hour urine creatinine clearance which is used in clinical practice, especially in the control and diabetic group.Istanbul Education and Research HospitalIstanbul Training & Research HospitalThis work was supported by Istanbul Education and Research Hospita

ALIŞILMADIK BİR HODGKİN DIŞI LENFOMA PREZENTASYONU: DİZDE LENFOMA

Agresif Hodgkin dışı lenfoma (HDL) olgularının %40’ını diffüz büyük B hücreli lenfoma (DBBHL) alt grubu oluşturur. DBBHL başlığı altında, klinik seyir, prognoz ve tedavi yanıtı bakımından farklılıklar gösteren bir dizi hastalık toplanmıştır; bunlar klinikte değişik bulgularla karşımıza çıkabilirler. Aşağıda sol dizindeki şişlik nedeniyle araştırılırken agresif HDL tanısı alan bir olgu sunulmuştur

MECP2 Spektrumundan Etkilenmiş 27 Olgunun Klinik ve Moleküler Bulguları

Giriş: MECP2-ilişkili fenotipler dişilerde tipik/atipik Rett sendromundan (RTT) hafif öğrenme güçlüğüne kadar değişkenlikgöstermekte, erkeklerde genellikle neonatal ensefalopati veya ağır sendromik/nonsendromik bilişsel gerilik ile seyretmektedir.RTT seyrinde sırasıyla regresyon, durağanlık ve kötüleşme evreleri görülmektedir. Sıklığı dişi doğumlarda 1/15-20000 olup fetalletalite nedeniyle erkeklerde çok nadirdir.Materyal-Metod: Çalışmamızda 2006-2020 yıllarında değerlendirilen, tipik/atipik RTT ve epileptik ensefalopati kliniğine sahip,karyotip sonucu normal, 25 dişi ve 2 erkek olgunun MECP2 Sanger dizileme ve multipleks ligasyon-bağımlı prob amplifikasyonu(MLPA) analizi sonuçları retrospektif değerlendirildi.Bulgular: Dişi olguların 3’ü atipik, 22’si tipik RTT, erkek olgular ise tipik RTT ve epileptik ensefalopati fenotiplerindeydi. Ortalama ilkbulgu yaşı 12 ay (0-19 ay), moleküler tanı yaşı 42/12 yaştı (22 ay-1710/12 yaş). Durağan dönemdeki 2 atipik, kötüleşme dönemindeki3 tipik RTT dişi olgu ve epileptik ensefalopatisi olan erkek olgu haricinde olgular regresyon döneminde tanı almışlardı. Olguların13’ünde anlamsız, 7’sinde yanlış anlamlı, 6’sında delesyon, 1’inde insersiyon-delesyon tipi değişim saptanmıştı. İki olguda 2novel değişim saptandı.Sonuç: Atipik RTT olguları ve 2 erkek olgunun geç tanı almasından yola çıkarak otizm spektrum bozukluğu ve özellikle erkeklerdeepileptik ensefalopati kliniğinde MECP2 spektrumunun akılda tutulmasının tanıdaki gecikmeyi önleyeceği düşünülmektedir.Anahtar Kelimeler: MECP2, Rett Sendrom