The 1996 outburst of GRO J1655-40: disc irradiation and enhanced mass transfer

We show that the 1996 outburst of the X-ray binary transient system GRO J1655-40 can be explained by the standard dwarf-nova type disc instability, followed by an episode of enhanced mass transfer from the secondary if the mass transfer rate in GRO J1655-40 is within a factor < 10 of the stability limit. We argue that irradiation of the secondary during the onset of the outburst driven by the thermal instability in the outer disc can increase the mass transfer rate above the minimum value required for stable accretion. This will then produce the period of near-constant X-ray emission seen in this system. This scenario can also explain the observed anti-correlation between the optical and X-ray fluxes. It is generally accepted that optical emission in low-mass X-ray binaries is produced by irradiation of the outer disc by X-rays. There is also strong circumstantial evidence that in order for the outer disc to see the irradiating flux, it must be warped. Depending on the warp propagation mechanism, either a burst of mass from the secondary or viscous decay are likely to decrease the degree of warping, thereby causing the decrease in the observed optical flux while the X-ray flux remains constant or even increases, exactly as observed in GRO J1655-40. Finally, the decrease of the disc warping and, therefore, irradiation will cause the disc to become unstable once again, terminating the outburst.Comment: Astronomy and Astrophysics - in pres

Modulation of Biological Responses to 2 ns Electrical Stimuli by Field Reversal

Nanosecond bipolar pulse cancellation, a recently discovered Phenomenon, is modulation of the effects of a unipolar electric pulse exposure by a second pulse of opposite polarity. This attenuation of biological response by reversal of the electric field direction has been reported with pulse durations from 60 ns to 900 ns for a wide range of endpoints, and it is not observed with conventional electroporation pulses of much longer duration (\u3e 100 mu s) where pulses are additive regardless of polarity. The most plausible proposed mechanisms involve the field-driven migration of ions to and from the membrane interface (accelerated membrane discharge). Here we report 2 ns bipolar pulse cancellation, extending the scale of previously published results down to the time required to construct the permeabilizing lipid electropores observed in molecular simulations. We add new cancellation endpoints, and we describe new bipolar pulse effects that are distinct from cancellation. This new data, which includes transport of cationic and anionic permeability indicators, fluorescence of membrane labels, and patterns of entry into permeabilized cells, is not readily explained by the accelerated discharge mechanism. We suggest that multi-step processes that involve first charged species movement and then responses of cellular homeostasis and repair mechanisms are more likely to explain the broad range of reported results

Dihydroethidium-Derived Fluorescence in Electrically Stressed Cells Indicates Intracellular Microenvironment Modifications Independent of ROS

Intracellular reactive oxygen species (ROS) generation is widely suggested as a trigger for biological consequences of electric field exposures, such as those in electroporation applications. ROS are linked with membrane barrier function degradation, genetic damage, and complex events like immunological cell death. Dihydroethidium (DHE) is commonly used to monitor ROS in cells. DHE is linked to intracellular ROS by a primary oxidation product, Ethidium (Eth+), that shows increased fluorescence upon binding to polynucleotides. We observed changes in DHE-derived fluorescence in Chinese hamster ovary (CHO) cells post 300-ns electric pulse exposures, comparing them to tert-butyl-hydroperoxide (t-BHP) induced oxidative stress. Immediate intracellular fluorescence changes were noted in both cases, but with distinct localization patterns. After electrical stress, cytosolic DHE-derived fluorescence intensity decreases, and nucleolar intensity increases. Conversely, t-BHP exposure increases DHE-derived fluorescence uniformly across the cell. Surprisingly, fluorescence patterns after electrical stress in Eth+-loaded cells is identical to those in DHE-loaded cells, in kinetics and localization patterns. These findings indicate that DHE-derived fluorescence changes after pulsed electric field stress are not due to intracellular ROS generation leading to DHE oxidation, but rather indicate stress-induced intracellular microenvironment alterations affecting Eth+ fluorescence

BVRI Light Curves for 22 Type Ia Supernovae

We present 1210 Johnson/Cousins B,V,R, and I photometric observations of 22 recent type Ia supernovae (SNe Ia): SN 1993ac, SN 1993ae, SN 1994M, SN 1994S, SN 1994T, SN 1994Q, SN 1994ae, SN 1995D, SN 1995E, SN 1995al, SN 1995ac, SN 1995ak, SN 1995bd, SN 1996C, SN 1996X, SN 1996Z, SN 1996ab, SN 1996ai, SN 1996bk, SN 1996bl, SN 1996bo, and SN 1996bv. Most of the photometry was obtained at the Fred Lawrence Whipple Observatory (FLWO) of the Harvard-Smithsonian Center for Astrophysics in a cooperative observing plan aimed at improving the data base for SN Ia. The redshifts of the sample range from $cz$=1200 to 37000 km s$^{-1}$ with a mean of $cz$=7000 km s$^{-1}$.Comment: Accepted to the Astronomical Journal, 41 pages, 8 figure

Microflares in accretion disks

We have investigated the phenomenon of explosive chromospheric evaporation from an accretion disk as a mechanism for fast variability in accreting sources such as low mass X-ray binaries and active galactic nuclei. This has been done in the context of advection dominated accretion flows, allowing both high and low states to be considered. This mechanism can in principle produce sub-millisecond timescales in binaries and sub-minute timescales in active galaxies. However, even considering the possibility that large numbers of these microflares may be present simultaneously, the power emitted from these microflares probably amounts to only a small fraction of the total X-ray luminosity.Comment: 5 pages, 1 figure, uses older A&A class file; accepted for publication in A&

Asymmetric Patterns of Small Molecule Transport After Nanosecond and Microsecond Electropermeabilization

Imaging of fluorescent small molecule transport into electropermeabilized cells reveals polarized patterns of entry, which must reflect in some way the mechanisms of the migration of these molecules across the compromised membrane barrier. In some reports, transport occurs primarily across the areas of the membrane nearest the positive electrode (anode), but in others cathode-facing entry dominates. Here we compare YO-PRO-1, propidium, and calcein uptake into U-937 cells after nanosecond (6 ns) and microsecond (220 µs) electric pulse exposures. Each of the three dyes exhibits a different pattern. Calcein shows no preference for anode- or cathode-facing entry that is detectable with our measurement system. Immediately after a microsecond pulse, YO-PRO-1 and propidium enter the cell roughly equally from the positive and negative poles, but transport through the cathode-facing side dominates in less than 1 s. After nanosecond pulse permeabilization, YO-PRO-1 and propidium enter primarily on the anode-facing side of the cell

Transport of Charged Small Molecules after Electropermeabilization - Drift and Diffusion

Background: Applications of electric-field-induced permeabilization of cells range from cancer therapy to wastewater treatment. A unified understanding of the underlying mechanisms of membrane electropermeabilization, however, has not been achieved. Protocols are empirical, and models are descriptive rather than predictive, which hampers the optimization and expansion of electroporation-based technologies. A common feature of existing models is the assumption that the permeabilized membrane is passive, and that transport through it is entirely diffusive. To demonstrate the necessity to go beyond that assumption, we present here a quantitative analysis of the post-permeabilization transport of three small molecules commonly used in electroporation research-YO-PRO-1, propidium, and calcein-after exposure of cells to minimally perturbing, 6 ns electric pulses. Results: Influx of YO-PRO-1 from the external medium into the cell exceeds that of propidium, consistent with many published studies. Both are much greater than the influx of calcein. In contrast, the normalized molar efflux of calcein from pre-loaded cells into the medium after electropermeabilization is roughly equivalent to the influx of YO-PRO-1 and propidium. These relative transport rates are correlated not with molecular size or cross-section, but rather with molecular charge polarity. Conclusions: This comparison of the kinetics of molecular transport of three small, charged molecules across electropermeabilized cell membranes reveals a component of the mechanism of electroporation that is customarily taken into account only for the time during electric pulse delivery. The large differences between the influx rates of propidium and YO-PRO-1 (cations) and calcein (anion), and between the influx and efflux of calcein, suggest a significant role for the post-pulse transmembrane potential in the migration of ions and charged small molecules across permeabilized cell membranes, which has been largely neglected in models of electroporation

Quantitative Limits on Small Molecule Transport via the Electropermeome - Measuring and Modeling Single Nanosecond Perturbations

The detailed molecular mechanisms underlying the permeabilization of cell membranes by pulsed electric fields (electroporation) remain obscure despite decades of investigative effort. To advance beyond descriptive schematics to the development of robust, predictive models, empirical parameters in existing models must be replaced with physics- and biology-based terms anchored in experimental observations. We report here absolute values for the uptake of YO-PRO-1, a small-molecule fluorescent indicator of membrane integrity, into cells after a single electric pulse lasting only 6 ns. We correlate these measured values, based on fluorescence microphotometry of hundreds of individual cells, with a diffusion-based geometric analysis of pore-mediated transport and with molecular simulations of transport across electropores in a phospholipid bilayer. The results challenge the drift and diffusion through a pore model that dominates conventional explanatory schemes for the electroporative transfer of small molecules into cells and point to the necessity for a more complex model

Spitzer Space Telescope Observations of Circumbinary Dust Disks around Polars

We present Spitzer Space Telescope IRAC photometry of the magnetic cataclysmic variables EF Eri, MR Ser, VV Pup, V834 Cen, GG Leo and V347 Pav. When we combine our results with the 2MASS data, we find that at least five of the polars have flux densities in the mid-IR in excess of the emission expected from the stellar components alone. We are unable to model this mid-IR excess with cyclotron emission, but we can recreate the observed spectral energy distributions with the inclusion of a simple circumbinary dust disk model. Importantly, we find that the masses of our modelled disks are approximately 12 orders of magnitude lower than required to significantly affect CV evolution. The accretion disk-less polars are ideal places to search for these disks, since the luminous accretion disk in most CVs would drown out the faint IR signature of the cooler, dimmer circumbinary disks

Scattered Emission from A Relativistic Outflow and Its Application to Gamma-Ray Bursts

We investigate a scenario of photons scattering by electrons within a relativistic outflow. The outflow is composed of discrete shells with different speeds. One shell emits radiation for a short duration. Some of this radiation is scattered by the shell(s) behind. We calculate in a simple two-shell model the observed scattered flux density as a function of the observed primary flux density, the normalized arrival time delay between the two emission components, the Lorentz factor ratio of the two shells and the scattering shell's optical depth. Thomson scattering in a cold shell and inverse Compton scattering in a hot shell are both considered. The results of our calculations are applied to the Gamma-Ray Bursts and the afterglows. We find that the scattered flux from a cold slower shell is small and likely to be detected only for those bursts with very weak afterglows. A hot scattering shell could give rise to a scattered emission as bright as the X-ray shallow decay component detected in many bursts, on a condition that the isotropically equivalent total energy carried by the hot electrons is large, $\sim 10^{52-56}$ erg. The scattered emission from a faster shell could appear as a late short $\gamma$-ray/MeV flash or become part of the prompt emission depending on the delay of the ejection of the shell.Comment: 13 pages, 3 figures, MNRAS in press; a short intuitive estimation is added before detailed calculations; references update