Le skarn à magnétite tardi-hercynien de Diélette (Massif armoricain, France)

National audienceLe " skarn ferrifère " de Diélette (Massif armoricain) s'est développé dans l'auréole de métamorphisme de contact du massif de granodiorite hercynien de Flamanville. L'encaissant gréso-pélitique dévonien inférieur s'est transformé en un ensemble de cornéennes, les niveaux carbonatés se transformant en grenatites à grossulaire (Gr64Ad27Py9 à Gr84Ad9Py7) et en pyroxénites à diopside - hédenbergite (Di65Hd35 à Di20Hd80) accompagnés d'un peu d'anorthite. Cette transformation se réalise lors de l'étape prograde, entre 390° et 525 °C, pour une pression estimée à 1 kbar. Cette étape a aussi transformé l'hématite primaire des bancs de grés oolithiques en magnétite, par métasomatose dès 500 °C, et sous des fO2 comprises entre 10-18 et 10-24 donnant naissance à la minéralisation ferrifère économique ; ce phénomène a pu se poursuivre lors de l'étape rétrograde. L'étape rétrograde est discrète et ne semble donner que des disséminations de sulfures de Fe-Cu-Mo. Elle se réalise sous des aS2 = 10-8 à 10-13 et des aO2 = 10-25 à 10-35 pour des températures inférieures à 390°- 400 °C. Ces très faibles valeurs, associées aux fortes valeurs de δ18O de la magnétite (+ 7,4 et + 10,7), suggèrent que la formation du skarn s'est réalisée sans la participation de fluides superficiels. On peut estimer à 20 000 ans la durée de vie du système hydrothermal à l'origine du skarn, un laps de temps cohérent avec la durée de refroidissement du massif (100 kans), et le volume de silice transporté (13 Mt). Diélette n'est pas un skarn ferrifère à proprement parler dans la mesure où un thermométamorphisme a simplement provoqué la transformation par métasomatose de niveaux ferrifères préexistants. La minéralisation véritablement de type skarn se limite aux disséminations et filonnets à molybdénite - scheelite observés sporadiquement dans l'auréole de contact

A Low Molecular Weight Protein from the Sea Anemone Anemonia viridis with an Anti-Angiogenic Activity

Sea anemones are a remarkable source of active principles due to a decentralized venom system. New blood vessel growth or angiogenesis is a very promising target against cancer, but the few available antiangiogenic compounds have limited efficacy. In this study, a protein fraction, purified from tentacles of Anemonia viridis, was able to limit endothelial cells proliferation and angiogenesis at low concentration (14 nM). Protein sequences were determined with Edman degradation and mass spectrometry in source decay and revealed homologies with Blood Depressing Substance (BDS) sea anemones. The presence of a two-turn alpha helix observed with circular dichroism and a trypsin activity inhibition suggested that the active principle could be a Kunitz-type inhibitor, which may interact with an integrin due to an Arginine Glycin Aspartate (RGD) motif. Molecular modeling showed that this RGD motif was well exposed to solvent. This active principle could improve antiangiogenic therapy from existing antiangiogenic compounds binding on the Vascular Endothelial Growth Factor (VEGF)

Homonuclear 1 H-NMR assignment and structural characterization of human immunodeficiency virus type 1 Tat Mal protein

International audienceThe transacting transcriptional activator (Tat) is a viral protein essential for activation of the human immunodeficiency virus (HIV) genes, and it plays an important role in HIV induced immunodeficiency. We report the NMR structural characterization of the active Tat Mal variant that belongs to a highly virulent D-subtype HIV type-1 (HIV-1) strain (Mal) found mainly in Africa. A full Tat Mal protein (87 residues) is synthesized. This synthetic protein is active in a transactivation assay with HeLa cells infected with the HIV long terminal repeated noncoding sequences of the HIV-1 provirus (LTR) lac Z gene. Homonuclear (1)H-NMR spectra allows the sequential assignment of the Tat Mal spin systems. Simulating annealing generates 20 conformers with similar folding. The geometry of the mean structure is optimized with energy minimization to obtain a final structure. As the European variant (Tat Bru) the N-terminal region of Tat Mal constitutes the core, and there is a hydrophobic pocket composed of the conserved Trp 11 interacting with several aromatic residues. The two functional regions of Tat (basic and the cysteine-rich regions) are well exposed to the solvent. A short alpha-helix is observed in region V adjacent to the basic region. This alpha helix induces local structural variations compared to the NMR structure of Tat Bru, and it brings the cysteine-rich and basic regions closer. This study suggests that similar folding exists among Tat variants

Genesis of the Paleoproterozoic Ammassalik Intrusive Complex, south-east Greenland

The Paleoproterozoic Ammassalik Intrusive Complex of south-east Greenland is located at the margin between the Rae Craton to the north and the North Atlantic Craton to the south. The complex is part of the Nagssugtoqidian Mobile Belt emplaced at mid- to lower-crustal level and represents an eastward continuation of the Nagssugtoqidian Orogen of western Greenland. We present new whole-rock geochemical and isotopic data (whole-rock Rb-Sr, Sm-Nd, U-Pb, Lu-Hf and zircon U-Pb, Lu-Hf, O) acquired from the Ammassalik Intrusive Complex, with the aim of reassessing its petrogenesis and evolution. The intrusive rocks and surrounding host-rocks show typical calc-alkaline signatures as well as negative Ta, Nb and Ti anomalies. The new U-Pb age data indicate a protracted magmatic history over ca. 40 Ma, involving at least three successive magmatic pulses between ca. 1910 and 1870 Ma emplaced into a granulite facies biotite-garnet paragneiss with ca. ≤ 1990 Ma protolith ages. Whole-rock trace element and isotope geochemistry indicate that the magmas that formed the Ammassalik Intrusive Complex were derived from a mildly enriched (εNd0 = −0.8 to –3.8; εSr0 = +24 to +42) and deep, garnet-bearing (MREE/HREE fractionated patterns) mantle source, possibly the sub-continental lithospheric mantle. However, the observed decreasing zircon εHf values with time (εHf0 = +2.8 to −12.5) indicate increasing degrees of crustal contamination involving variably 18O-enriched crustal material (δ18O = 7–9‰) into the mafic magmas of the complex. This interpretation suggests that the mantle-derived magma do not have a deep, garnet-bearing source, but that the continental arc signature in the Ammassalik Intrusive Complex igneous rocks may have been inherited from the assimilation of partial melts of the garnet-bearing host-rocks. In addition, based on the new crystallisation ages broadly coeval with the Columbia supercontinent build up, marked by the formation of world-class Ni-Cu deposits globally, it is proposed that the Ammassalik Intrusive Complex and the south-east Greenland region were in direct continuity of the mineralised Kotalahti belt (Svecofennian Province, Finland). Together with the recent discovery of Ni-Cu mineralisation in the Ammassalik Intrusive Complex, our study further supports the high prospectivity of south-east Greenland for orthomagmatic Ni-Cu deposits

H-1-C-13 nuclear magnetic resonance assignment and structural characterization of HIV-1 Tat protein

Tat is a viral protein essential for activation of the HIV genes and plays an important role in the HIV-induced immunodeficiency. We chemically synthesized a Tat protein (86 residues) with its six glycines Ca labelled with C-13. This synthetic protein has the full Tat activity. Heteronuclear nuclear magnetic resonance (NMR) spectra and NOE back-calculation made;possible the sequential assignment of the 86 spin systems. Consequently, 915 NMR restraints were identified and 272 of them turned out to be long range ([i-j] > 4), providing structural information on the whole Tat protein. The poor spectral dispersion of Tat NMR spectra does not allow an accurate structure to be determined as for other proteins studied by 2D NMR. Nevertheless, we were able to determine the folding for Tat protein at a 1-mM protein concentration in a 100 mM, pH 4.5 phosphate buffer. The two main Tat functional regions, the basic region and the cysteine-rich region, are well exposed to solvent while a part of the N-terminal region and the C-terminal region constitute the core of Tat Bru. The basic region adopts an extended structure while the cysteine-rich region is made up of two loops. Resolution of this structure was determinant to develop a drug design approach against Tat. The chemical synthesis of the drugs allowed the specific binding and the inhibition of Tat to be verified. (C) 2000 Academie des sciences/Editions scientifiques et medicales Elsevier SAS.status: publishe

Mineralisation footprints and regional timing of the world-class Siguiri orogenic gold district (Guinea, West Africa)

Siguiri is a world-class orogenic gold district hosted in the weakly metamorphosed Upper Birimian to Lower Tarkwa Group sedimentary rocks of the Siguiri Basin (Guinea). The district is characterised by a protracted deformation history associated with four main deformation events: D1S is a N-S compression; D2S is an E-W compression progressively evolving into an early-D3S transpression and then into a late-D3S NNW-SSE transtension and D4S is a NE-SW compression. Field observations, petrography and geochemistry at three key deposits of the Siguiri district (Bidini, Sintroko PB1 and Kosise) suggest a polyphase hydrothermal history that can be subdivided into four hydrothermal events. The first hydrothermal event was associated with the development of barren bedding-parallel and en-echelon V2S quartz-dominated-(pyrite) veins. The second hydrothermal event is characterised by the development of V3A pyrite-ankerite veins late during D3S. Laser ablation-ICP-MS data show that this vein set contains high gold contents of up to 43.3 ppm, in substitution in pyrite crystal lattice, representing a minor first gold mineralisation event. The third and most prominently developed hydrothermal event is late D3S and represents the second and principal gold mineralisation event. This mineralisation event led to two distinct mineralisation textures. The first texture is best exposed in the Kosise deposit and is characterised by gold-bearing quartz-ankerite-arsenopyrite conjugate V3B veins. Although the bulk of the gold is hosted in native gold grains in V3B veins, LA-ICP-MS analyses show that gold also substitutes in the arsenopyrite crystal lattice (up to 55.5 ppm). The second mineralisation texture is best expressed in the Sanu Tinti deposit and consists of disseminated barren pyrite hosted in a polymict conglomerate. The second and third hydrothermal events are both structurally controlled by a series of early-D3S N-S, NE-SW, WNW-ESE and E-W sub-vertical incipient structures expressed as fracture zones of higher V3S vein density. A composite geochemical cross section across fracture zones from the Kosise deposit indicates that gold mineralisation in the Siguiri district is associated with enrichments in Ag, Au, As, Bi, Co, Mo, (Sb), S, Te and W relative to background. Geochemical variations associated with the ore shoots in the Siguiri district are consistent with petrographic observations and highlight an albite-carbonate-sulphide-sericite alteration. The fourth and last hydrothermal event is associated with the development of a late penetrative S4S cleavage during D4S deformation, which overprints all pre-existing hydrothermal features and is associated with the deposition of free gold, chalcopyrite and galena along fractures in V3A pyrite and V3B pyrite and arsenopyrite. Mineralogical and geochemical footprints as well as timing of the gold-mineralising events in the Siguiri district, when compared with other deposits of the West African Craton, highlight the synchronicity of gold mineralisation in Siguiri (syn-D3S and syn-D4S events) with other similar events in this part of the craton, such as the early Au-Sb-Bi-(Te-W) mineralisation at the Morila deposit in Southeast Mali. Our results support the hypothesis that late Eburnean-age gold mineralisation in the Siguiri district and in the West African Craton as a whole was polyphase