Miokarditis vezan uz slinavku i šap u sisajuće teladi.

Foot-and-mouth disease (FMD) can lead to myocarditis in young animals, but the age distributions of calves with myocarditis have not been described, nor the biochemical profile in these calves. In an area endemic with foot-and-mouth disease, calves less than 6 months of age in infected farms were examined for clinical lesions and abnormalities in respiratory rate, heart rate and heart rhythm. In total, 53 calves were identified to be suspected of having foot-and-mouth disease infection. In 6 calves myocarditis was suspected based on tachypnea, tachycardia and gallop rhythm. In these 6 calves, cardiac troponin-I (cTnI) and aspartate aminotransferase (AST) were significantly higher (P<0.0001), but the levels of Creatinine Kinase MB (CK-MB) and Lactate dehydrogenase (LDH) were not. These 6 calves died within 2 days and histopathology confirmed myocarditis. All calves with myocarditis were younger than 2-months old, suggesting that myocarditis caused by FMD is mainly found in very young suckling calves.Slinavka i šap može dovesti do miokarditisa u mladih životinja. Dosada nije opisana dobna raspodjela miokarditisa ni biokemijski profil u teladi oboljele od slinavke i šapa. U jednom području gdje se slinavka i šap javlja endemijski, telad mlađa od šest mjeseci bila je na zaraženim farmama klinički pretražena posebice na poremećaje u frekvenciji bila, disanja i srčanog ritma. Ukupno su 53 teleta bila sumnjiva na slinavku i šap. Sumnja na miokarditis postavljena je u šest teladi i to na osnovi tahipneje, tahikardije i galopirajućeg ritma. U te su teladi razine srčanog troponina-I (cTnI) i aspartat-aminotransferaze (AST) bile značajno više (P<0,0001), dok razine kreatinin-kinaze MB (CK-MB) i laktat-dehidrogenaze (LDH) nisu. Tih šest teleta uginulo je unutar dva dana te je u njih miokarditis bio potvrđen patohistološki. Sva telad s miokarditisom bila je mlađa od dva mjeseca, što upućuje na zaključak da se miokarditis uzrokovan virusom slinavke i šapa pretežito javlja u sisajuće teladi najranije dobi

Citološka analiza endometrija kod krava s akutnim i kroničnim endometritisom.

The aim of the present study was to evaluate the findings of endometrial cytology in cows with acute and chronic endometritis. For this purpose samples were collected from 217 Brown Swiss and Holstein cows, housed on the Atatürk University Dairy Research Farm between the years 2010 and 2012, and they were stained with Giemsa for cytological examination. In the evaluation, overall 100 cells were counted in the microscopic area and the cells were classified as polymorph nuclear leukocyte, macrophage, lymphocyte and epithelial cells. The cytopathological classification was done according to the percentages of inflammatory cells. Briefly, 126 (58.06 %) samples had extensive inflammatory cells, and of the uterine samples 91 (41.94 %) had normal exfoliation. According to the cellular density results, acute, and chronic and subacute endometritis were described in 68 (31.33 %), 23 (10.60 %) and 35 (16.13 %) cases, respectively. In conclusion, endometrial cytology was found to be an applicable and reliable diagnostic method in diagnosis and diffentiation of acute and chronic endometritis.Cilj istraživanja bio je procijeniti citološke nalaze u endometriju krava s akutnim i kroničnim endometritisom. U tu su svrhu između 2010. i 2012. godine bili prikupljeni uzorci od 217 krava smeđe i holštajnske pasmine, uzgajanih na pokusnoj mliječnoj farmi na Sveučilištu Atatürk. Uzorci su bili obojeni Giemsinim bojenjem. Pri procjeni je u vidnom polju mikroskopa bilo izbrojeno ukupno 100 stanica kojesu razvrstane u polimorfononuklearne leukocite, makrofage, limfocite i epitelne stanice. Citopatološko razvrstavanje bilo je provedeno na osnovi postotka upalnih stanica. Ukratko, upalne stanice bile su ustanovljene u 126 (58,06 %) uzoraka, dok je u 91 (41,94 %) uzorku maternice ustanovljeno normalno ljuštenje stanica. Na osnovi stanične gustoće, akutni endometritis bio je dokazan u 68 (31,33 %) uzoraka, kronični u 23 (10,60 %), a subakutni u 35 (16,13 %) uzoraka. Zaključno, citologija endometrija pokazala se primjenjljivom i pouzdanom metodom u dijagnostici odnosno razlikovanju akutnog od kroničnog endometritisa

Is It Possible to Treat Equine Papillomatosis with Ivermectin Given Orally?

This case report aims to evaluate the effect of ivermectin in the treatment of a horse with papillomatosis. The horse was a 14-month-old mare, with cutaneous papillomatosis diagnosed by clinical and histopahological examinations. The history revealed that papillomas had developed and progressed in the last 2 months. A single shot of ivermectin (Eqvalan paste(R), Topkim. Turkey) at a dose of 200 mcg/kg was orally administered to the horse. The horse was observed at 7(th), 15(th) and 30(th) days and no other treatment during the recovery period. Papillomas regressed clearly on the 7(th) day after the administration of ivermectin treatment and regressed nearly 90% on the 15(th) day and, showed complete remission on the 30(th) day. The results obtained in this case indicated that ivermectin might be effect in the treatment of equine papillomatosis

Protective Effect of Grape Seed Extract on Doxorubicin-Induced Nephrotoxicity and Hepatotoxicity in Albino Mice

ORUC, Ertan/0000-0003-4234-8219WOS: 000284394200003The aim of this study was to evaluate the possible nephroprotective and hepatoprotective effects of grape seed extract (GSE), an antioxidant agent, against doxorubicin (DOX)-induced nephrotoxicity and hepatotoxicity in mice using biochemical and histopathological approaches. Swiss albino mice were randomly divided into six groups, each consisting of six animals: control; DOX (2.5 mg/kg body weight, 6-days cumulative dosage 15 mg/kg b. wt); GSE (50 mg/kg b. wt); GSE (150 mg/kg b. wt); GSE (50 mg/kg b. wt) + DOX; and GSE (150 mg/kg b. wt) + DOX. Serum aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen and creatinine levels were determined to assess the liver and kidney functions, respectively. Besides, the liver and kidney samples were taken for the determination of tissue malondialdehyde and glutathione levels, as well as histopathological changes in the tissues. The results indicated that DOX-induced oxidative damage caused a significant decrease in glutathione levels, but a significant increase in malondialdehyde levels of the liver and kidney tissues. Each dose of GSE provided significant protection against DOX-induced toxicity, and the strongest effect was observed at a dose of 150 mg/kg body weight. Histopathological studies showed that DOX caused some structural damages, such as vascular congestion, hepatocyte and tubular epithelial degeneration, hemorrhage, glomerular atrophy and necrosis, hyaline casts in kidney, and fibrosis in the liver and kidney. In vivo results showed that GSE extract is a potent protector against DOX-induced nephrotoxicity and hepatotoxicity

Protective Effect of Royal Jelly on Some Biochemical Parameters in Cadmium-Treated Albino Mice

ORUC, Ertan/0000-0003-4234-8219WOS: 000284394200005In the present study, the protective role of Royal Jelly (RJ) on cadmium-induced toxicity in albino mice was investigated. The mice were divided into six groups. The control group received tap water, mice of treatment groups received 2 mg/kg body weight cadmium and two different doses of RJ (100 and 250 mg/kg b. wt) for 6 weeks by oral gavage. The levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine were evaluated. In addition, histopathological changes in liver and kidneys were investigated. Serum levels of AST, ALT, ALP, BUN and creatinine in mice receiving cadmium alone significantly increased compared to the other groups (p<0.05). Treatment with RJ produced amelioration in serum biochemical indices according to cadmium group. Each dose of RJ provided protection against cadmium toxicity, and its strongest protective effect was observed at a dose of 250 mg/kg b. wt. In conclusion, RJ can act as a protector agent against cadmium-induced damages of liver and kidney.Giresun University Scientific Research Projects DepartmentGiresun UniversityThis study was supported by grants from Giresun University Scientific Research Projects Department

Diagnosis of Campylobacteriosis in the Aborted Bovine Foetuses by Pathological, Immunohistochemical, Microbiological and Real Time PCR

WOS: 000278340800026In this study; 105 aborted bovine fetuses were examined for Campylobacteriosis and pathologic findings were compared with microbiologic, immunohistochemical and real time polimerase chain reaction (Real Time-PCR) methods. Although, Campylobacter spp. was microbiologically detected in only 4 cases, this was detected in 5 cases by immunohistochemical method and by Real Time-PCR in 7 cases. It is concluded that 6.6% (7/105) of abortion cases were caused by Campylobacteriosis and Real Time PCR more sensitive and usefull for the the diagnosis of the disease and immunohistochemical method is as sensitive as microbiologic methods for diagnosis in unsufficient labarotories

Myocarditis associated with foot-and-mouth disease in suckling calves

Foot-and-mouth disease (FMD) can lead to myocarditis in young animals, but the age distributions of calves with myocarditis have not been described, nor the biochemical profile in these calves. In an area endemic with foot-and-mouth disease, calves less than 6 months of age in infected farms were examined for clinical lesions and abnormalities in respiratory rate, heart rate and heart rhythm. In total, 53 calves were identified to be suspected of having foot-and-mouth disease infection. In 6 calves myocarditis was suspected based on tachypnea, tachycardia and gallop rhythm. In these 6 calves, cardiac troponin-I (cTnI) and aspartate aminotransferase (AST) were significantly higher (PFoot-and-mouth disease (FMD) can lead to myocarditis in young animals, but the age distributions of calves with myocarditis have not been described, nor the biochemical profile in these calves. In an area endemic with foot-and-mouth disease, calves less than 6 months of age in infected farms were examined for clinical lesions and abnormalities in respiratory rate, heart rate and heart rhythm. In total, 53 calves were identified to be suspected of having foot-and-mouth disease infection. In 6 calves myocarditis was suspected based on tachypnea, tachycardia and gallop rhythm. In these 6 calves, cardiac troponin-I (cTnI) and aspartate aminotransferase (AST) were significantly higher (P&lt;0.0001), but the levels of Creatinine Kinase MB (CK-MB) and Lactate dehydrogenase (LDH) were not. These 6 calves died within 2 days and histopathology confirmed myocarditis. All calves with myocarditis were younger than 2-months old, suggesting that myocarditis caused by FMD is mainly found in very young suckling calves.</p

The effects of grape seed extract against toxicity of benzene on liver and kidney tissues of albino mice: biochemical evaluation

ORUC, Ertan/0000-0003-4234-8219WOS: 000351543800007Objective: The objective of the present study was to investigate the possible effects of grape seed extract (GSE) against benzene-induced toxicity in Swiss albino mice. Methods: The animals were randomly divided into six groups each containing six mice. Group I, treated with distilled water; Group II and III orally treated with 50 mg/kg and 150 mg/kg body weight GSE, respectively. Group IV, orally treated with 250 mg/kg body weight benzene by using feeding cannula; Group V, orally treated with 50 mg/kg body weight GSE + 250 mg/kg body weight benzene; Group VI, orally treated with 150 mg/kg body weight GSE + 250 mg/kg of body weight benzene for 50 consecutive days. At the end of experimental period all mice were sacrificed; blood, liver and kidney tissues were removed after post-mortem examination. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine levels were analyzed from serum. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were analyzed from isolated tissues. And also histopathological examinations of liver and kidney tissues were investigated. Results: Serum AST, ALT, ALP, BUN and creatinine levels were slightly increased in Group IV compared with the other tested groups (p<.05). Benzene-induced toxicity caused a significant decrease in GSH levels and a significant rise in MDA levels of liver and kidney tissues. Oral treatment with GSE significantly ameliorated the indices of hepatotoxicity, nephrotoxicity and lipid peroxidation induced by benzene. Both doses of GSE provided significant protection and the strongest effects were observed at the dose level of 150 mg/kg. Conclusion: Consequently, it was found that GSE has a significant positive effect in benzene-induced toxicity, and its GSE effect is dose dependent

The Protective Effects of Peganum harmala Extract on Lung and Kidney in Sepsis Induced by Cecal Ligation and Perforation in Rats

Sepsis is characterized by multiple organ dysfunction, tissue damage and hyper-inflammation. Peganum harmala (PH) is a plant considered for its antibacterial, antioxidant, anticarcinogen and antiinflammatory properties. This study was aimed to evaluate the protective effects of PH extract on tissues and cytokines in sepsis induced by cecal ligation and perforation (CLP) in rats. Forty rats were divided into five groups. Groups were sham-operated (control), CLP, 90 mg/kg PH-treated CLP, 180 mg/kg PH-treated CLP and 180 mg/kg PH-treated control healthy. Animals were sacrificed at the 16th h of the study. Biochemical and histopathological analyses were performed in lung, kidney and blood samples. Both 90 mg/kg and 180 mg/kg doses of PH decreased the level of interleukin-1 beta (IL-1 beta) and interleukin-10 (IL-10), and high dose of PH reduced the tumor necrosis factor-alpha (TNF-alpha) in the serum compared to CLP group. The PH also increased the activity of superoxide dismutase (SOD) and the total levels of glutathione (GSH) in the lung and kidney tissues of septic rats. The level of malondialdehyde (MDA) in the lung and kidney tissues was reduced in both PH treated CLP groups. The histopathological results were in accordance with the biochemical results. The CLP + 180 mg/kg PH group had the lowest inflammation score in the lung. In conclusion, the administration of PH has prevented the oxidative stress, the cytokine response and the inflammation in CLP-induced septic rats

Cadmium Induced Changes on Growth Performance, Some Biochemical Parameters and Tissue in Broilers: Effects of Vitamin C and Vitamin E

ORUC, Ertan/0000-0003-4234-8219;WOS: 000294653200005This study was performed to determine the effects of vitamin C (vit C) and vitamin E (vit E) on growth performance, some biochemical parameters and tissue changes in broiler chickens exposed to cadmium (Cd). One hundred twenty, 1-day-old, Ross broiler chicks were divided into 4 groups with 3 replicates and fed with a basal diet (control) or the basal diet supplemented with 60 mg kg(-1) Cd, 60 mg kg(-1) Cd+400 mg kg(-1) vit C, or 60 mg kg(-1) Cd+250 mg kg(-1) vit E for 42 days. Body weight (BW), Body Weight Gain (BWG) and Feed Consumption (FC) were recorded and feed conversion ratio (FCR) was calculated. Plasma were analysed for aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyltransferase (GGT) activities and total protein, total cholesterol and creatinine levels. Cadmium significantly decreased the BW (p<0.001), BWG (p<0.001), FC (p<0.001) and FCR (p<0.05) compared with the control group. Regarding the same groups plasma and kidney Cd (p<0.001) and AST (p<0.05) and GGT activities (p<0.01) were increased. While growth performance were not affected in vit C and vit E supplementated group compared with the Cd-only group, these treatments reduced plasma Cd and GGT activities. Furthermore vit C was more effective in reducing Cd accumulation in kidneys and liver.Kirikkale UniversityKirikkale University [2005/10]This study was supported by the Kirikkale University Research Fund, Project No: 2005/10. Some part of the data of this article was presented as an abstract at the 3 Veterinary Biochemistry and Clinic Biochemistry Congress. Konya, Turkey