Impact of multiple radar reflectivity data assimilation on the numerical simulation of a flash flood event during the HyMeX campaign

An analysis to evaluate the impact of multiple radar reflectivity data with a three-dimensional variational (3-D-Var) assimilation system on a heavy precipitation event is presented. The main goal is to build a regionally tuned numerical prediction model and a decision-support system for environmental civil protection services and demonstrate it in the central Italian regions, distinguishing which type of observations, conventional and not (or a combination of them), is more effective in improving the accuracy of the forecasted rainfall. In that respect, during the first special observation period (SOP1) of HyMeX (Hydrological cycle in the Mediterranean Experiment) campaign several intensive observing periods (IOPs) were launched and nine of which occurred in Italy. Among them, IOP4 is chosen for this study because of its low predictability regarding the exact location and amount of precipitation. This event hit central Italy on 14 September 2012 producing heavy precipitation and causing several cases of damage to buildings, infrastructure, and roads. Reflectivity data taken from three C-band Doppler radars running operationally during the event are assimilated using the 3-D-Var technique to improve high-resolution initial conditions. In order to evaluate the impact of the assimilation procedure at different horizontal resolutions and to assess the impact of assimilating reflectivity data from multiple radars, several experiments using the Weather Research and Forecasting (WRF) model are performed. Finally, traditional verification scores such as accuracy, equitable threat score, false alarm ratio, and frequency bias - interpreted by analysing their uncertainty through bootstrap confidence intervals (CIs) - are used to objectively compare the experiments, using rain gauge data as a benchmark

Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma

BACKGROUND: Growing evidence is showing that metastatic cell populations are able to transfer their characteristics to less malignant cells. Exosomes (EXOs) are membrane vesicles of endocytic origin able to convey their cargo of mRNAs, microRNAs (miRs), proteins and lipids from donors to proximal as well as distant acceptor cells. Our previous results indicated that miR-221&222 are key factors for melanoma development and dissemination. The aim of this study was to verify whether the tumorigenic properties associated with miR-222 overexpression can be also propagated by miR-222-containing EXOs. METHODS: EXOs were isolated by UltraCentrifugation or Exoquick-TC(®) methods. Preparations of melanoma-derived vesicles were characterized by using the Nanosight™ technology and the expression of exosome markers analyzed by western blot. The expression levels of endogenous and exosomal miRNAs were examined by real time PCR. Confocal microscopy was used to evaluate transfer and uptake of microvesicles from donor to recipient cells. The functional significance of exosomal miR-222 was estimated by analyzing the vessel-like process formation, as well as cell cycle rates, invasive and chemotactic capabilities. RESULTS: Besides microvesicle marker characterization, we evidenced that miR-222 exosomal expression mostly reflected its abundance in the cells of origin, correctly paralleled by repression of its target genes, such as p27Kip1, and induction of the PI3K/AKT pathway, thus confirming its functional implication in cancer. The possible differential significance of PI3K/AKT blockade was assessed by using the BKM120 inhibitor in miR-222-transduced cell lines. In addition, in vitro cultures showed that vesicles released by miR-222-overexpressing cells were able to transfer miR-222-dependent malignancy when taken-up by recipient primary melanomas. Results were confirmed by antagomiR-221&222 treatments and by functional observations after internalization of EXOs devoid of these miRs

Towards a RISC-V Open Platform for Next-generation Automotive ECUs

The complexity of automotive systems is increasing quickly due to the integration of novel functionalities such as assisted or autonomous driving. However, increasing complexity poses considerable challenges to the automotive supply chain since the continuous addition of new hardware and network cabling is not considered tenable. The availability of modern heterogeneous multi-processor chips represents a unique opportunity to reduce vehicle costs by integrating multiple functionalities into fewer Electronic Control Units (ECUs). In addition, the recent improvements in open-hardware technology allow to further reduce costs by avoiding lock-in solutions. This paper presents a mixed-criticality multi-OS architecture for automotive ECUs based on open hardware and open-source technologies. Safety-critical functionalities are executed by an AUTOSAR OS running on a RISC-V processor, while the Linux OS executes more advanced functionalities on a multi-core ARM CPU. Besides presenting the implemented stack and the communication infrastructure, this paper provides a quantitative gap analysis between an HW/SW optimized version of the RISC-V processor and a COTS Arm Cortex-R in terms of real-time features, confirming that RISC-V is a valuable candidate for running AUTOSAR Classic stacks of next-generation automotive MCUs.Comment: 8 pages, 2023 12th Mediterranean Conference on Embedded Computing (MECO

Pathologist's approach to paediatric and neonatal eosinophilic gastrointestinal disorders

Children are not simply miniature adults. The evaluation of their gastrointestinal disorders is therefore different from that in full-grown adults and requires a particular clinical/pathologic approach. : Different studies have tried to assess the normal eosinophil distribution in the gastrointestinal tract in adults while very few studies have investigated the paediatric population, consequently complicating the pathologist's ability in identifying an abnormal number of eosinophils in this setting of patients. : When evaluating gastrointestinal tract biopsies with eosinophilia, eosinophilic count must be considered along with other histological features like eosinophil distribution in the gastrointestinal wall, their degranulation, cryptitis and crypt abscesses, other accompanying inflammatory cells, apoptotic bodies, foreign material or microorganisms; these findings, although rarely specific, may be a useful aid for diagnosis. : Reports should not include a diagnosis of primary eosinophilic gastrointestinal disorders (EoGID) if clinical data and test results do not rule out other forms of gastrointestinal eosinophilia. A more descriptive definition like "with eosinophilic pattern" should be favoured over a specific diagnosis of "eosinophilic disorder" in order to avoid potential confusion between different entities

The comparative analysis of Mediterranean coastal communities: six case studies

The aim of this study is to promote cooperation and actions for the benefit of coastal communities on the Southern and Eastern shores of the Mediterranean region by adopting an approach that integrates environmental, economic and social dimensions. These areas are traditionally based on Fisheries, especially small-scale fisheries (SSFs), which contribute to strengthen social cohesion, in that the seafood value chains still represent the backbone of the coastal economy. The six coastal communities analysed in this paper are located in Algiers port - Casbah (Algeria), Marsa Matrouh (Egypt), Tricase (Italy), Tyre (Lebanon), Nador Lagoon (Morocco), Zarzis (Tunisia). Conclusions emphasise the need to develop a comprehensive reference system for dialogue, cooperation and capacity building both at national and regional level. The cluster approach can help create a favourable cooperation and competition environment, generating income and employment opportunities for local communities.Le but de cette \ue9tude est de promouvoir la coop\ue9ration et des actions en faveur des communaut\ue9s c\uf4ti\ue8res sur les rives Sud et Est de la M\ue9diterran\ue9e, en adoptant une approche qui int\ue8gre les dimensions environnementale, \ue9conomique et sociale. Cette r\ue9gion est traditionnellement ax\ue9e sur la p\ueache, et notamment la petite p\ueache, qui contribue \ue0 renforcer la coh\ue9sion sociale, car les cha\ueenes de valeur des produits de la mer constituent encore la colonne vert\ue9brale de l\u2019\ue9conomie c\uf4ti\ue8re. Les six communaut\ue9s c\uf4ti\ue8res examin\ue9es dans ce travail sont situ\ue9es \ue0 la Casbah- port d\u2019Alger (Alg\ue9rie), \ue0 Marsa Matrouh (Egypte), \ue0 Tricase (Italie), Tyre (Liban), dans la lagune de Nador (Maroc) et \ue0 Zarzis (Tunisie). Dans les conclusions, l\u2019accent est mis sur l\u2019importance de structurer un syst\ue8me de r\ue9f\ue9rence global pour encourager le dialogue, la coop\ue9ration et le d\ue9veloppement des capacit\ue9s \ue0 l\u2019\ue9chelle nationale et r\ue9gionale. Le mod\ue8le du cluster pourrait contribuer \ue0 cr\ue9er un environnement de coop\ue9ration et comp\ue9tition favorable, g\ue9n\ue9rant des revenus et des emplois au niveau des communaut\ue9s locales

Automatic Detection of Myotonia using a Sensory Glove with Resistive Flex Sensors and Machine Learning Techniques

This paper deals with the automatic detection of Myotonia from a task based on the sudden opening of the hand. Data have been gathered from 44 subjects, divided into 17 controls and 27 myotonic patients, by measuring a 2-point articulation of each finger thanks to a calibrated sensory glove equipped with a Resistive Flex Sensor (RFS). RFS gloves are proven to be reliable in the analysis of motion for myotonic patients, which is a relevant task for the monitoring of the disease and subsequent treatment. With the focus on a healthy VS pathological comparison, customized features were extracted, and several classifications entailing motion data from single fingers, single articulations and aggregations were prepared. The pipeline employed a Correlation-based feature selector followed by a SVM classifier. Results prove that it’s possible to detect Myotonia, with aggregated data from four fingers and upper/lower articulations providing the most promising accuracies (91.1%

Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post‐Mortem Brain of Schizophrenia, Parkinson’s and Alzheimer’s Disease Patients

Schizophrenia (SCZ) is a mental illness characterized by aberrant synaptic plasticity and connectivity. A large bulk of evidence suggests genetic and functional links between postsynaptic abnormalities and SCZ. Here, we performed quantitative PCR and Western blotting analysis in the dorsolateral prefrontal cortex (DLPFC) and hippocampus of SCZ patients to investigate the mRNA and protein expression of three key spine shapers: the actin‐binding protein cyclase‐associated protein 2 (CAP2), the sheddase a disintegrin and metalloproteinase 10 (ADAM10), and the synapse-associated protein 97 (SAP97). Our analysis of the SCZ post‐mortem brain indicated increased DLG1 mRNA in DLPFC and decreased CAP2 mRNA in the hippocampus of SCZ patients, compared to non‐psychiatric control subjects, while the ADAM10 transcript was unaffected. Conversely, no differences in CAP2, SAP97, and ADAM10 protein levels were detected between SCZ and control individuals in both brain regions. To assess whether DLG1 and CAP2 transcript alterations were selective for SCZ, we also measured their expression in the superior frontal gyrus of patients affected by neurodegenerative disorders, like Parkinson’s and Alzheimer’s disease. Interestingly, also in Parkinson’s disease patients, we found a selective reduction of CAP2 mRNA levels relative to controls but unaltered protein levels. Taken together, we reported for the first time altered CAP2 expression in the brain of patients with psychiatric and neurological disorders, thus suggesting that aberrant expression of this gene may contribute to synaptic dysfunction in these neuropathologies

Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post‐Mortem Brain of Schizophrenia, Parkinson’s and Alzheimer’s Disease Patients

Schizophrenia (SCZ) is a mental illness characterized by aberrant synaptic plasticity and connectivity. A large bulk of evidence suggests genetic and functional links between postsynaptic abnormalities and SCZ. Here, we performed quantitative PCR and Western blotting analysis in the dorsolateral prefrontal cortex (DLPFC) and hippocampus of SCZ patients to investigate the mRNA and protein expression of three key spine shapers: the actin‐binding protein cyclase‐associated protein 2 (CAP2), the sheddase a disintegrin and metalloproteinase 10 (ADAM10), and the synapse-associated protein 97 (SAP97). Our analysis of the SCZ post‐mortem brain indicated increased DLG1 mRNA in DLPFC and decreased CAP2 mRNA in the hippocampus of SCZ patients, compared to non‐psychiatric control subjects, while the ADAM10 transcript was unaffected. Conversely, no differences in CAP2, SAP97, and ADAM10 protein levels were detected between SCZ and control individuals in both brain regions. To assess whether DLG1 and CAP2 transcript alterations were selective for SCZ, we also measured their expression in the superior frontal gyrus of patients affected by neurodegenerative disorders, like Parkinson’s and Alzheimer’s disease. Interestingly, also in Parkinson’s disease patients, we found a selective reduction of CAP2 mRNA levels relative to controls but unaltered protein levels. Taken together, we reported for the first time altered CAP2 expression in the brain of patients with psychiatric and neurological disorders, thus suggesting that aberrant expression of this gene may contribute to synaptic dysfunction in these neuropathologies