Cryptic receptors for insulin-like growth factor II in the plasma membrane of rat adipocytes — a possible link to cellular insulin resistance

AbstractTo further elucidate the mechanisms for short-term regulation of the receptor for insulin-like growth factor II (IGF-II), we investigated effects of insulin, cAMP and phosphatase inhibitors on cell surface 125I-IGF-II binding in rat adipocytes. Preincubation with the serine/threonine phosphatase inhibitor okadaic acid (OA, 1 μMM) or the non-hydrolysable cAMP analogue N6-mbcAMP (4 mM) markedly impaired insulin-stimulated 125I-IGF-II binding. Furthermore, addition of OA enhanced the inhibitory effect exerted by N6-mbcAMP. N6-mbcAMP also induced an insensitivity to insulin which was normalized by concomitant addition of the tyrosine phosphatase inhibitor vanadate (0.5 mM). In contrast, vanadate did not affect the impairment in maximal insulin-stimulated 25I-IGF-II binding produced by either OA or N6-mbcAMP. Phospholipase C (PLC), which cleaves phospholipids at the cell surface, markedly enhanced cell surface 125I-IGF-II binding in a concentration-dependent manner. Scatchard analysis demonstrated that the effect of PLC was due to an increased number of binding sites suggesting that ‘cryptic’ IGF-II receptors are associated with the plasma membrane (PM). PLC (5 U/ml) also reversed the N6-mbcAMP-induced decrease of 125I-IGF-II binding at a low insulin concentration (10 μU/ml). Taken together, these data indicate that CAMP, similar to its effects on the glucose transporter GLUT 4 and the insulin receptor, may increase the proportion of functionally cryptic IGF-II receptors in the PM through mechanisms involving serine phosphorylation, possibly of a docking or coupling protein. Tyrosine phosphorylation appears to exert an opposite effect promoting the full cell surface expression of receptors

Continuous downstream processing of a monoclonal antibody using Periodic Counter Current Chromatography (PCC) and Straight Through Processing (STP)

There is an increased interest to perform process intensification in order to reduce costs and improve throughput in the development and production of monoclonal antibodies (MAbs). One solution to these demands can be to implement continuous or semi-continuous downstream processing. New emerging technologies such as periodic counter-current (PCC) chromatography and straight through processing (STP) are entering the market. Here, these two technologies were evaluated in a continuous three step chromatography MAb process. The capture step was performed with protein A media (resin) on a 3 column PCC chromatography system followed by two polishing steps which were connected in series with an in line conditioning step in between. The three step process was performed using MAb from fed-batch cell culture. Results will also be presented based on the purification of MAb from a perfusion cell culture using PCC setup for the capture step

Distriktssköterskans upplevelse av att använda motiverande samtal i sitt dagliga arbete

Ohälsosamma levnadsvanor leder till hälsoproblem. En stor del av distriktssköterskans arbete handlar om hälsofrämjande arbete. Motiverande samtal (MI) är en samtalsmetodik där distriktssköterskan i samarbete med patienten kan locka fram patientens inre motivation till förändring. Syftet var att beskriva distriktssköterskans upplevelse av att använda MI i det dagliga arbetet på vårdcentral. Intervjuer gjordes med tolv distriktssköterskor i Skåne. Resultatet analyserades med innehållsanalys. Resultatet visade att distriktssköterskorna upplevde MI som ett bra reskap samtidigt som det framkom svårigheter att använda sig av metodens verktyg. MI upplevdes som användbart för olika patientgrupper. Dock efterfrågades mer träning och fortbildning i MI. Slutsats är att MI ger goda förutsättningar att lyckas motivera patienter. Dock efterfrågas ett bättre samarbete mellan yrkeskategorier för ett gemensamt förhållningssätt gentemot patienten i förändringsprocessen

Technology to Support Children\u27s Social Care: Opportunities and Challenges

The potential for information and communication technology (ICT) to support the delivery of social services, and the possible benefits afforded, have been acknowledged in numerous studies. The many obstacles to the adoption and integration of ICT into social services have also been documented. This paper provides a summary of those issues as the backdrop to the description of a study conducted to understand the adoption of a specific technology (OmMej) in the context of children’s social care in Sweden. This study looks at the perceived benefits provided through the use of OmMej, particularly in terms of the opportunity for children to have a voice in their care and the impact on this technology on social work practice. The study also identifies barriers to the successful deployment of the tool, and some lessons learned that can inform other implementation efforts. drawing to explore international student experience in Scotland. Historically rich pictures are difficult to interpret and are often used to gain a holistic understanding of a system of concern and thus are disregarded in terms of providing in-depth qualitative data. We will explore the use of inter-coder content analysis to gain a deep understanding of group thinking. In the context of this study, using content analysis, our findings revealed a detailed understanding of Scottish culture and traditions from the perspective of international students. We determine that visuals have a vast capacity to communicate, irrespective of possible language, culture and education barriers, and thus offer unique insight into a complex system of stakeholder understanding

Plan&Do - A Technology Probe Supporting Children with Intellectual Disabilities in Leisure Activities

In this paper, we discuss possibilities for how digital tools can support children with intellectual disabilities to carry out leisure activities. Leisure activities play an important role for physiological and psychological well-being, but children with intellectual disabilities carry out less leisure activities than others. In order to investigate what factors are important for supporting children with intellectual disabilities to carry out leisure activities, we have developed a mobile application, Plan&Do, acting as a technology probe. The contribution of this paper is based on results from a situated evaluation, and consist of a raised awareness of the many challenges parents and children face when choosing and preparing for taking part in a leisure activity, as well as early results from how this can be supported, and directions for future work

In-hospital medication reviews reduce unidentified drug-related problems

Purpose To examine the impact of a new model of care, in which a clinical pharmacist conducts structured medication reviews and a multi-professional team collates systematic medication care plans, on the number of unidentified DRPs in a hospital setting. Methods In a prospective two-period study, patients admitted to an internal medicine ward at the University Hospital of Lund, Sweden, were included if they were >= 65 years old, used >= 3 medications on a regular basis and had stayed on the ward for >= 5 weekdays. Intervention patients were given the new model of care and control patients received conventional care. DRPs were then retrospectively identified after study completion from blinded patient records for both intervention and control patients. Two pairs of evaluators independently evaluated and classified these DRPs as having been identified/unidentified during the hospital stay and according to type and clinical significance. The primary endpoint was the number of unidentified DRPs, and the secondary endpoints were the numbers of unidentified DRPs within each type and clinical significance category. Results The study included a total of 141 (70 intervention and 71 control) patients. The intervention group benefited from a reduction in the total number of unidentified DRPs per patient during the hospital stay: intervention group median 1 (1st-3rd quartile 0-2), control group 9 (6-13.5) (p < 0.001), and also in the number of medications associated with unidentified DRPs per patient: intervention group 1 (0-2), control group 8 (5-10) (p < 0.001). All sub-categories of DRPs that were frequent in the control group were significantly reduced in the intervention group. Similarly, the DRPs were less clinically significant in the intervention group. Conclusions A multi-professional team, including a clinical pharmacist, conducting structured medication reviews and collating systematic medication care plans proved very effective in reducing the number of unidentified DRPs for elderly in-patients

An autocrine sphingosine-1-phosphate signaling loop enhances NF-kappaB-activation and survival

Peer reviewe

Pitkäaikaissairaiden näkemyksiä omasta ja terveydenhuollon ammattilaisten osallistumisesta lääkehoitonsa toteutukseen

English summaryPeer reviewe

Маркеры воспаления при атеросклеротических изменениях сосудистой стенки у пациентов с ишемической болезнью сердца

ишемическая болезнь сердцаатеросклерозцитокин