Perancangan Sistem Dan Denah Bazaar Dengan Memanfaatkan Canvas HTML5 Berbasis Website Dan Android

At present, almost every organizers holds an attractive bazaar due to the growing number of enthusiasts who want to join bazaar. Bazaar can be an alternative place to sell new product and introduce the local product to the general society. Booking booth process in bazaar still using manual system, which tenant fills the order form and then the form is scanned to be sent to the organizer of the event via e-email. Once the booking is received, event organizer has to check and make a registration list of tenants whom will join the bazaar. Therefore there is a need to have an information system to help both eventorganizer and tenant to make a better booking process. Aplication Bazaar based on Android and website intends to help user do all booking process efficiently.Application based on mobile assist tenant in making reservation from fill form until payment order. It helps tenant to search for information about the bazaar which will be held in accordance with bazaar categories of interest and can follow event, so tenant will be easier to get bazaar information. In addition, application based on website helps event organizer to manage the whole process from booking to the payment which received from tenant order, then event organizer can edit event map in order to facilitate tenant when choose a booth at the bazaar.The test results which obatained have two modules, Mobile Application based on Android for tenant and application website for event organizer. Mobile Application can make a booking in bazaar, can get a notification, follow the event, displaying order data. Application website for the event organizer can manage the whole order and payment from tenant, and can edit map event to help tenant make an order. From the test of mobile application that have been done, 50% of respondents rate the app is overall good, and 42% of respondent rate the app overall very good. For Website Application, event organizer has satified with the existing features on the website

Evaluation of flight efficiency for Stockholm Arlanda Airport arrivals

Analysis of punctuality of airport arrivals, as well as identification of causes of the delays within transition airspace, is an important step in evaluating performance of the Terminal Maneuvering Area (TMA) Air Navigation Services: without knowing the current performance levels, it is difficult to identify which areas could be improved. Deviations from the flight plans is one of the major reasons for arrival delays. In this work, we quantified the impact of the deviations from the flight plans on the fuel burn. One of the main reasons of fuel waste is non- optimal vertical profiles during the descent phase. We calculated how much extra fuel is wasted due to vertical flight inefficiency within Stockholm TMA.Peer ReviewedPostprint (published version

Using Fluctuation Analysis to Establish Causal Relations between Cellular Events without Experimental Perturbation

AbstractExperimental perturbations are commonly used to establish causal relationships between the molecular components of a pathway and their cellular functions; however, this approach suffers inherent limitations. Especially in pathways with a significant level of nonlinearity and redundancy among components, such perturbations induce compensatory responses that obscure the actual function of the targeted component in the unperturbed pathway. A complementary approach uses constitutive fluctuations in component activities to identify the hierarchy of information flow through pathways. Here, we review the motivation for using perturbation-free approaches and highlight recent advances made in using perturbation-free fluctuation analysis as a means to establish causality among cellular events

Probabilistic modeling and analysis of the effects of extra-cellular matrix density on the sizes, shapes, and locations of integrin clusters in adherent cells

<p>Abstract</p> <p>Background</p> <p>Regulation of integrin binding to the specific complementary sites on extra-cellular matrix (ECM) proteins plays a major role in cell adhesion and migration. In addition to regulating single integrin-ligand bonds by affinity modulation, cells regulate their adhesiveness by forming integrin clusters. Although it is clear that cells exhibit different adhesion and migration behaviors on surfaces coated with different concentrations of ECM proteins, it is not clear if this response is mediated by changes in the availability of integrin binding sites or by differential intracellular signaling that may affect integrin binding and clustering.</p> <p>Results</p> <p>To quantify how the concentration of ECM affects integrin clustering, we seeded cells expressing the integrin αIIbβ3 on different concentrations of the complementary ECM protein fibrinogen (Fg) and measured the resulting integrin cluster properties. We observed heterogeneity in the properties of integrin clusters, and to characterize this population heterogeneity we use a probabilistic modeling approach to quantify changes to the distributions of integrin cluster size, shape, and location.</p> <p>Conclusions</p> <p>Our results indicate that in response to increasing ECM density cells form smaller integrin clusters that are less elongated and closer to the cell periphery. These results suggest that cells can sense the availability of ECM binding sites and consequently regulate integrin clustering as a function of ECM density.</p

Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy

AbstractThe use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical limits on the imaging field of view size. Here, we present a light sheet fluorescence microscope that achieves 390 nm isotropic resolution and high optical sectioning strength (i.e., out-of-focus blur is strongly suppressed) over large field of views, without the need for structured illumination or deconvolution-based postprocessing. We demonstrate simultaneous dual-color, high-contrast, and high-dynamic-range time-lapse imaging of migrating cells in complex three-dimensional microenvironments, three-dimensional tracking of clathrin-coated pits, and long-term imaging spanning >10 h and encompassing >2600 time points

In Chemotaxing Fibroblasts, Both High-Fidelity and Weakly Biased Cell Movements Track the Localization of PI3K Signaling

Cell movement biased by a chemical gradient, or chemotaxis, coordinates the recruitment of cells and collective migration of cell populations. During wound healing, chemotaxis of fibroblasts is stimulated by platelet-derived growth factor (PDGF) and certain other chemoattractants. Whereas the immediate PDGF gradient sensing response has been characterized previously at the level of phosphoinositide 3-kinase (PI3K) signaling, the sensitivity of the response at the level of cell migration bias has not yet been studied quantitatively. In this work, we used live-cell total internal reflection fluorescence microscopy to monitor PI3K signaling dynamics and cell movements for extended periods. We show that persistent and properly aligned (i.e., high-fidelity) fibroblast migration does indeed correlate with polarized PI3K signaling; accordingly, this behavior is seen only under conditions of high gradient steepness (>10% across a typical cell length of 50 μm) and a certain range of PDGF concentrations. Under suboptimal conditions, cells execute a random or biased random walk, but nonetheless move in a predictable fashion according to the changing pattern of PI3K signaling. Inhibition of PI3K during chemotaxis is accompanied by loss of both cell-substratum contact and morphological polarity, but after a recovery period, PI3K-inhibited fibroblasts often regain the ability to orient toward the PDGF gradient.National Science Foundation (U.S.) (0828936)National Institutes of Health (U.S.) (GM074711)National Institutes of Health (U.S.) (EB007280

Stochastic model of integrin-mediated signaling and adhesion dynamics at the leading edges of migrating cells.

Productive cell migration requires the spatiotemporal coordination of cell adhesion, membrane protrusion, and actomyosin-mediated contraction. Integrins, engaged by the extracellular matrix (ECM), nucleate the formation of adhesive contacts at the cell's leading edge(s), and maturation of nascent adhesions to form stable focal adhesions constitutes a functional switch between protrusive and contractile activities. To shed additional light on the coupling between integrin-mediated adhesion and membrane protrusion, we have formulated a quantitative model of leading edge dynamics combining mechanistic and phenomenological elements and studied its features through classical bifurcation analysis and stochastic simulation. The model describes in mathematical terms the feedback loops driving, on the one hand, Rac-mediated membrane protrusion and rapid turnover of nascent adhesions, and on the other, myosin-dependent maturation of adhesions that inhibit protrusion at high ECM density. Our results show that the qualitative behavior of the model is most sensitive to parameters characterizing the influence of stable adhesions and myosin. The major predictions of the model, which we subsequently confirmed, are that persistent leading edge protrusion is optimal at an intermediate ECM density, whereas depletion of myosin IIA relieves the repression of protrusion at higher ECM density