Investigating the Role of Phox2B-expressing Glutamatergic Parafacial Zone Neurons in Sleep Wake Control

Inhibitory GABAergic neurons in the parafacial zone (PZGABA) are essential for slow wave sleep (SWS). Since existing literature about the heterogenous population of PZ neurons is lacking, questions remain regarding the non-GABAergic sleep active PZ neurons. This study seeks to determine if glutamatergic PZ neurons expressing the transcription factor Phox2B (PZPhox2B) participate in sleep-wake control. Phox2B-IRES-Cre mice received injections of adeno-associated virus containing Cre-dependent diphtheria toxin subunit A (DTA) DNA into the PZ (PZPhox2B-DTA). Analysis of injection sites revealed transfection covering the PZ and the locus coeruleus, also known to express Phox2B. We recorded the sleep-wake cycle of PZPhox2B-DTA mice and compared them with control mice, analyzing their sleep-wake quantity, fragmentation, and power spectral distribution. We found total amounts and cortical power for wakefulness, SWS, and REM sleep of PZPhox2B-DTA mice were unaffected. There was fragmentation in wakefulness during the active period for PZPhox2B-DTA mice, seen as a significant reduction in the amount of time and number of episodes spent in the longest bout; however, wakefulness during the rest period was not significantly altered. No significant change was found in the bout numbers and amounts for SWS and REM sleep of PZPhox2B-DTA mice. I was unable to confirm targeted ablation of PZPhox2B-DTA neurons due to a lack of reliable antibody staining. Therefore, it remains possible that ablation of PZPhox2B neurons was incomplete and the wakeful fragmentation is due to neuronal ablation outside of the PZ, such as in the neighboring LC

Direct combination: a new user interaction principle for mobile and ubiquitous HCI

Direct Combination (DC) is a recently introduced user interaction principle. The principle (previously applied to desktop computing) can greatly reduce the degree of search, time, and attention required to operate user interfaces. We argue that Direct Combination applies particularly aptly to mobile computing devices, given appropriate interaction techniques, examples of which are presented here. The reduction in search afforded to users can be applied to address several issues in mobile and ubiquitous user interaction including: limited feedback bandwidth; minimal attention situations; and the need for ad-hoc spontaneous interoperation and dynamic reconfiguration of multiple devices. When Direct Combination is extended and adapted to fit the demands of mobile and ubiquitous HCI, we refer to it as Ambient Combination (AC) . Direct Combination allows the user to exploit objects in the environment to narrow down the range of interactions that need be considered (by system and user). When the DC technique of pairwise or n-fold combination is applicable, it can greatly lessen the demands on users for memorisation and interface navigation. Direct Combination also appears to offers a new way of applying context-aware information. In this paper, we present Direct Combination as applied ambiently through a series of interaction scenarios, using an implemented prototype system

Novel steady state of a microtubule assembly in a confined geometry

We study the steady state of an assembly of microtubules in a confined volume, analogous to the situation inside a cell where the cell boundary forms a natural barrier to growth. We show that the dynamical equations for growing and shrinking microtubules predict the existence of two steady states, with either exponentially decaying or exponentially increasing distribution of microtubule lengths. We identify the regimes in parameter space corresponding to these steady states. In the latter case, the apparent catastrophe frequency near the boundary was found to be significantly larger than that in the interior. Both the exponential distribution of lengths and the increase in the catastrophe frequency near the cell margin is in excellent agreement with recent experimental observations.Comment: 8 pages, submitted to Phys. Rev.

Surface Roughness Dominated Pinning Mechanism of Magnetic Vortices in Soft Ferromagnetic Films

Although pinning of domain walls in ferromagnets is ubiquitous, the absence of an appropriate characterization tool has limited the ability to correlate the physical and magnetic microstructures of ferromagnetic films with specific pinning mechanisms. Here, we show that the pinning of a magnetic vortex, the simplest possible domain structure in soft ferromagnets, is strongly correlated with surface roughness, and we make a quantitative comparison of the pinning energy and spatial range in films of various thickness. The results demonstrate that thickness fluctuations on the lateral length scale of the vortex core diameter, i.e. an effective roughness at a specific length scale, provides the dominant pinning mechanism. We argue that this mechanism will be important in virtually any soft ferromagnetic film.Comment: 4 figure

Parallel developmental genetic features underlie stickleback gill raker evolution.

BackgroundConvergent evolution, the repeated evolution of similar phenotypes in independent lineages, provides natural replicates to study mechanisms of evolution. Cases of convergent evolution might have the same underlying developmental and genetic bases, implying that some evolutionary trajectories might be predictable. In a classic example of convergent evolution, most freshwater populations of threespine stickleback fish have independently evolved a reduction of gill raker number to adapt to novel diets. Gill rakers are a segmentally reiterated set of dermal bones important for fish feeding. A previous large quantitative trait locus (QTL) mapping study using a marine × freshwater F2 cross identified QTL on chromosomes 4 and 20 with large effects on evolved gill raker reduction.ResultsBy examining skeletal morphology in adult and developing sticklebacks, we find heritable marine/freshwater differences in gill raker number and spacing that are specified early in development. Using the expression of the Ectodysplasin receptor (Edar) gene as a marker of raker primordia, we find that the differences are present before the budding of gill rakers occurs, suggesting an early change to a lateral inhibition process controlling raker primordia spacing. Through linkage mapping in F2 fish from crosses with three independently derived freshwater populations, we find in all three crosses QTL overlapping both previously identified QTL on chromosomes 4 and 20 that control raker number. These two QTL affect the early spacing of gill raker buds.ConclusionsCollectively, these data demonstrate that parallel developmental genetic features underlie the convergent evolution of gill raker reduction in freshwater sticklebacks, suggesting that even highly polygenic adaptive traits can have a predictable developmental genetic basis

Capturing an Evolving Nebular Environment: A Petrographic and Geochemical Study of a Type A, B & C CAI

Calcium, Aluminum-rich Inclusions (CAIs) were the first formed solids in our Solar System, with mineral assemblages reflecting the first phases predicted to condense out of a hot nebular gas of Solar composition. Geochemical, textural and crystallographic information in CAIs can be used to constrain the temperature, pressure, and composition (e.g., oxygen fugacity) of the gaseous reservoir(s) from which they formed, as well as any secondary (nebular and parent body) processes they underwent. Coordinated geochemical and textural analyses provide information on nebular conditions (i.e., astrophysical environments and dynamics of nebular gas reservoirs) in which these CAIs formed. In order to better understand the evolution of nebular reservoirs at the time of CAI formation, we analyzed a Type A, B and C CAI using Electron Probe Micro-Analyzer (EPMA) and Electron BackScatter Diffraction (EBSD) at NASA Johnson Space Center (JSC)

Constraining the Temperature of Impact Melt from the Mistastin Lake Impact Structure Using Zircon Crystal Structures

Impact melt is a product of hyper-velocity impact events formed by the instantaneous melting of near-surface target rocks. Constraining the temperature of impact melt is vital to understanding its prograde heating and cooling history, which can have implications for inferring the environment of early Earth ~4.0 billion years ago when microbial life potentially arose. To date, only one datum on the initial impact melt temperature has been derived by Timms et al. These authors studied zirconia microstructures and crystallographic orientations that revealed the former presence of cubic zirconia, found in a black impact glass at the Mistastin Lake impact structure, Canada. The presence of cubic zirconia indicates a minimum temperature for the impact melt of >2370C from the dissociation temperature of zircon to cubic zirconia and liquid SiO2. With only one temperature datum, it is still difficult to constrain the entire temperature range experienced during the impact melting process; from its instantaneous formation to thermal equilibrium with the cold clasts collected along the crater floor and walls. In addition, obtaining a temperature value from only one type of impactite limits the inferred temperature range, because each impactite experiences a different cooling history. In this study, we present a preliminary investigation of 61 zircon crystals, 14 of which are similar to those studied by Timms et al., from the Mistastin Lake impact structure. To acquire a more accurate temperature profile representative of impact melt, zircon crystals were collected from different types of impactites containing impact melt, including additional samples of the black impact glass studied by Timms et al

Radio Recombination Lines at Decametre Wavelengths: Prospects for the Future

This paper considers the suitability of a number of emerging and future instruments for the study of radio recombination lines (RRLs) at frequencies below 200 MHz. These lines arise only in low-density regions of the ionized interstellar medium, and they may represent a frequency-dependent foreground for next-generation experiments trying to detect H I signals from the Epoch of Reionization and Dark Ages ("21-cm cosmology"). We summarize existing decametre-wavelength observations of RRLs, which have detected only carbon RRLs. We then show that, for an interferometric array, the primary instrumental factor limiting detection and study of the RRLs is the areal filling factor of the array. We consider the Long Wavelength Array (LWA-1), the LOw Frequency ARray (LOFAR), the low-frequency component of the Square Kilometre Array (SKA-lo), and a future Lunar Radio Array (LRA), all of which will operate at decametre wavelengths. These arrays offer digital signal processing, which should produce more stable and better defined spectral bandpasses; larger frequency tuning ranges; and better angular resolution than that of the previous generation of instruments that have been used in the past for RRL observations. Detecting Galactic carbon RRLs, with optical depths at the level of 10^-3, appears feasible for all of these arrays, with integration times of no more than 100 hr. The SKA-lo and LRA, and the LWA-1 and LOFAR at the lowest frequencies, should have a high enough filling factor to detect lines with much lower optical depths, of order 10^-4 in a few hundred hours. The amount of RRL-hosting gas present in the Galaxy at the high Galactic latitudes likely to be targeted in 21-cm cosmology studies is currently unknown. If present, however, the spectral fluctuations from RRLs could be comparable to or exceed the anticipated H I signals.Comment: 9 pages; Astron. & Astrophys., in pres