The Macquenoise sandstone : a suitable Lochkovian raw material for ancient millstones : quarries, properties, manufacture and distribution (Belgium-France)

International audienceSince a few years a French-Belgian team of archeologists and geologists work on the characterization of ancient quern stones and millstones (mainly from the Antique period) linked with the identification of their stratigraphical and geographical provenances.This fruitful collaboration aims at reconstructing the ancient commercial roads and economic organization of territories during that period.In addition to the better-known volcanic rocks from the Eifel area, our study revealed the frequent occurrence of particular Devonian conglomerates and sandstones from the Ardenne area, as raw materials for the manufacturing of querns and millstones. The latter sandstones as well as the related antique millstone quarries, form an important and until now undervalued geological and cultural heritage in Belgium.Near the Ardenne border between France and Belgium, the earliest Devonian (Lochkovian) sediments deposited on the Caledonian substrate are conglomerates, which are interpreted as continental alluvial fans. The first marine sediments are littoral sandstones/quartzites or shales/slates. A sandstone formation, formally defined as the “Arkose d’Haybes” is closely linked to the former Lochkovian conglomerates (called also the “Poudingue de Fépin”). The outcrops of this particular formation around the reference localities of Haybes, Fépin and Hargnies (Ardennes, France), show a partly recrystallized, well-sorted grey to greenish sandstone (turbidite facies) with wine-red coloured joints. Another important area displaying old quarries in the same formation is located between Hirson (Aisne, France) and Macquenoise (Hainaut, Belgium): here, a more homogenous grey coarse sandstone facies occurs, with a better consistency and small amounts of dark green tourmaline crystals.These detrital formations were locally quarried, not only for building stones but also for the production of querns and millstones. The huge Proto-historical and Roman millstone quarries in Lochkovian conglomerates and sandstones from Macquenoise are known since the 19th century. Even if the lack of recent archeological excavations do not allow yet to precise the exact age of their quarrying, the dating of the various products derived from these quarries allow to consider the duration of the active extraction and how far the millstones from Macquenoise area were transported into the northern Gallo-Roman provinces

Assessing the influence of DEM source on derived streamline and catchment boundary accuracy

Accurate DEM-derived streamlines and catchment boundaries are essential for hydrological modelling. Due to the popularity of hydrological parameters derived mainly from free DEMs, it is essential to investigate the accuracy of these parameters. This study compared the spatial accuracy of streamlines and catchment boundaries derived from available digital elevation models in South Africa. Two versions of Stellenbosch University DEMs (SUDEM5 and DEMSA2), the second version of the 30 m advanced spaceborne thermal emission and reflection radiometer global digital elevation model (ASTER GDEM2), the 30 and 90 m shuttle radar topography mission (SRTM30 and SRTM90 DEM), and the 90 m Water Research Commission DEM (WRC DEM) were considered. As a reference, a 1 m GEOEYE DEM was generated from GeoEye stereo images. Catchment boundaries and streamlines were extracted from the DEMs using the Arc Hydro module. A reference catchment boundary was generated from the GEOEYE DEM and verified during field visits. Reference streamlines were digitised at a scale of 1:10 000 from the 1 m orthorectified GeoEye images. Visual inspection, as well as quantitative measures such as correctness index, mean absolute error, root mean squares error and figure of merit index were used to validate the results. The study affirmed that high resolution (<30 m) DEMs produce more accurate parameters and that DEM source and resampling techniques also play a role. However, if high resolution DEMs are not available, the 30 m SRTM DEM is recommended as its vertical accuracy was relatively high and the quality of the streamlines and catchment boundary was good. In addition, it was found that the novel Euclidean distancebased MAE and RMSE proposed in this study to compare reference and DEM-extracted raster datasets of different resolutions is a more reliable indicator of geometrical accuracy than the correctness and figure of merit indices.Keywords: hydrology, catchment delineation, digital elevation model, correctness index, figure of merit index, Euclidean distance inde

The immune response against Chlamydia suis genital tract infection partially protects against re-infection

International audienceThe aim of the present study was to reveal the characteristic features of genital Chlamydia suis infection and re-infection in female pigs by studying the immune response, pathological changes, replication of chlamydial bacteria in the genital tract and excretion of viable bacteria. Pigs were intravaginally infected and re-infected with C. suis strain S45, the type strain of this species. We demonstrated that S45 is pathogenic for the female urogenital tract. Chlamydia replication occurred throughout the urogenital tract, causing inflammation and pathology. Furthermore, genital infection elicited both cellular and humoral immune responses. Compared to the primo-infection of pigs with C. suis, re-infection was characterized by less severe macroscopic lesions and less chlamydial elementary bodies and inclusions in the urogenital tract. This indicates the development of a certain level of protection following the initial infection. Protective immunity against re-infection coincided with higher Chlamydia-specific IgG and IgA antibody titers in sera and vaginal secretions, higher proliferative responses of peripheral blood mononuclear cells (PBMC), higher percentages of blood B lymphocytes, monocytes and CD8+ T cells and upregulated production of IFN-γ and IL-10 by PBMC

Linkage and continuity of care after release from prison : an evaluation of Central Registration Points for drug users in Belgium

Purpose This paper aims to report the findings of an evaluation study concerning the Central Registration Points (CRPs) for drug users in Belgian prisons. CRPs support drug users to link with community-based services. Design/methodology/approach The study applied a multi-method approach that involved an exploratory literature review; a secondary analysis of the CRPs' databases; a qualitative study of the perceptions of a diverse sample of stakeholders with regard to the functioning of CRPs; and a prospective registration study. Findings One-third of the clients never attended an outpatient or residential substance abuse service before prison entry. This illustrates that the CRPs managed to reach clients who were not previously reached by (substance abuse) treatment services. All interviewed actors emphasized the added value of the CRPs in terms of informing, contacting, motivating and referring prisoners with a substance abuse problem. Practical implications Based on the research findings, two issues seem to be of paramount importance in the successful practice of CRPs: the confidentiality and specific expertise on (substance abuse) treatment. Given the complex situation of drug users in prison, an independent positioning and categorical assistance with drug-specific expertise seem to be essential. Originality/value CRPs can be considered to be one of the "building blocks" that contribute to high-quality care and continuity of care for drugs users in detention

Pharmacokinetic and urinary profiling reveals the prednisolone/cortisol ratio as a valid biomarker for prednisolone administration

Background: In Europe, synthetic corticosteroids are not allowed in animal breeding for growth-promoting purposes. Nevertheless, a high prevalence of non-compliant urine samples was recently reported for prednisolone, however, without any indication of unauthorized use. Within this context, 20 beta-dihydroprednisolone and the prednisolone/cortisol ratio have been suggested as potential tools to discriminate between exogenous and endogenous urinary prednisolone. In this study, the validity of these strategies was verified by investigating the plasma pharmacokinetic and urinary excretion profiles of relevant glucocorticoids in bovines, subjected to exogenous prednisolone treatment or tetracosactide hexaacetate administration to induce endogenous prednisolone formation. Bovine urine and plasma samples were analysed by liquid chromatography and mass spectrometry. Results: Based on the plasma pharmacokinetics and urinary profiles, 20 beta-dihydroprednisolone was confirmed as the main prednisolone-derived metabolite, being detected in the biological fluids of all 12 bovines (plasma AUC(0-inf) of 121 h mu g L-1 and urinary concentration > 0.695 mu g L-1). However, this metabolite enclosed no potential as discriminative marker as no significant concentration differences were observed upon exogenous prednisolone treatment or tetracosactide hexaacetate administration under all experimental conditions. As a second marker tool, the prednisolone/cortisol ratios were assessed along the various treatments, taking into account that endogenous prednisolone formation involves the hypothalamic-pituitary-adrenal axis and is associated with an increased cortisol secretion. Significantly lower ratios were observed in case of endogenous prednisolone formation (i.e. ratios ranging from 0.00379 to 0.129) compared to the exogenous prednisolone treatment (i.e. ratios ranging from 0.0603 to 36.9). On the basis of these findings, a discriminative threshold of 0.260 was proposed, which allowed classification of urine samples according to prednisolone origin with a sensitivity of 94.2% and specificity of 99.0%. Conclusion: The prednisolone/cortisol ratio was affirmed as an expedient strategy to discriminate between endogenous and exogenous prednisolone in urine. Although the suggested threshold value was associated with high specificity and sensitivity, a large-scale study with varying experimental conditions is designated to optimize this value

The macquenoise sandstone (Devonian – Lochkovian), a suitable raw material for ancient querns and millstones : quarries, properties, manufacture and distribution in France and Belgium

For some years, a French-Belgian team of archaeologists and geologists is investigating the provenance of ancient quern-stones and millstones. Their study revealed the frequent occurrence of particular coarse sandstones derived from Lower Devonian strata in the Ardenne region, known as either the “Arkose of Haybes” by geologists or the “Arkose of Macquenoise” by archaeologists. Material for Late Iron Age and Roman quern-stones and millstones was quarried from open pits located west of the border between France and Belgium, between the Belgian village of Macquenoise (Commune of Momignies, Province of Hainaut) and the French town of Hirson (Aisne Department, Hauts-de-France region). This paper describes the raw materials, presents the different types of grindstones produced through historical times and provides a detailed diffusion map of the millstones

Chlamydia trachomatis L2c infection in a porcine model produced urogenital pathology and failed to induce protective immune responses against re-infection

The current study was designed to evaluate the pathogenesis, pathology and immune response of female genital tract infection with Chlamydia trachomatis L2c, the most recently discovered lymphogranuloma venereum strain, using a porcine model of sexually transmitted infections. Pigs were mock infected, infected once or infected and re-infected intravaginally, and samples were obtained for chlamydial culture, gross and microscopic pathology, and humoral and cell-mediated immunity. Intravaginal inoculation of pigs with this bacterium resulted in an infection that was confined to the urogenital tract, where inflammation and pathology were caused that resembled what is seen in human infection. Re-infection resulted in more severe gross pathology than primary infection, and chlamydial colonization of the urogenital tract was similar for primary infected and re- infected pigs. This indicates that primary infection failed to induce protective immune responses against re-infection. Indeed, the proliferative responses of mononuclear cells from blood and lymphoid tissues to C. trachomatis strain L2c were never statistically different among groups, suggesting that C. trachomatis-specific lymphocytes were not generated following infection or re-infection. Nevertheless, anti-chlamydial antibodies were elicited in sera and vaginal secretions after primary infection and re-infection, clearly resulting in a secondary systemic and mucosal antibody response. While primary infection did not protect against reinfection, the porcine model is relevant for evaluating immune and pathogenic responses for emerging and known C. trachomatis strains to advance drug and/or vaccine development in humans

The most likely time and place of introduction of BTV8 into belgian ruminants

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