Data from: miR-9a modulates maintenance and ageing of Drosophila germline stem cells by limiting N-cadherin expression

Ageing is characterized by a decline in stem cell functionality leading to dampened tissue regeneration. While the expression of microRNAs across multiple species is markedly altered with age, the mechanism by which they govern stem cell-sustained tissue regeneration is unknown. We report that in the Drosophila testis, the conserved miR-9a is expressed in germline stem cells and its levels are significantly elevated during ageing. Transcriptome and functional analyses show that miR-9a directly regulates the expression of the adhesion molecule N-cadherin (N-cad). miR-9a null mutants maintain a higher number of stem cells even in the aged tissue. Remarkably, this rise fails to improve tissue regeneration and results in reduced male fertility. Similarly, overexpression of N-cad also results in elevated stem cell number and decreased regeneration. We propose that miR-9a downregulates N-cad to enable adequate detachment of stem cells toward differentiation, thus providing the necessary directionality toward terminal differentiation and spermatogenesis

RNA-Seq - w1118 30 days Replicate #1 Lane 1 Read 1

RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #1, Lane 1, Read

RNA-Seq - mir9a KO 1 day Replicate #2 Lane 1 Read 1

RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #2, Lane 1, Read

RNA-Seq - mir9a KO 30 days Replicate #2 Lane 1 Read 1

RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #2, Lane 1, Read

RNA-Seq - w1118 30 days Replicate #2 Lane 1 Read 1

RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2, Lane 1, Read

RNA-Seq - w1118 30 days Replicate #2 Lane 2 Read 1

RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2, Lane 2, Read

RNA-Seq - mir9a KO 1 day Replicate #2 Lane 1 Read 2

RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 1 day, Replicate #2, Lane 1, Read

RNA-Seq - mir9a KO 30 days Replicate #1 Lane 2 Read 1

RNA-Sequencing data from Drosophila testes, Genotype mir9a knockout, Age 30 days, Replicate #1, Lane 2, Read

RNA-Seq - w1118 30 days Replicate #2 Lane 1 Read 2

RNA-Sequencing data from Drosophila testes, Genotype w1118, Age 30 days, Replicate #2, Lane 1, Read