Population structure and genetic diversity of Streptococcus suis isolates obtained from the United States

Diseases caused by the zoonotic pathogen Streptococcus suis are an extensive economic problem as well as an animal welfare concern for the global swine industry. Previous studies have evaluated the genomic diversity and population structure of S. suis isolates, however, the majority of these studies utilized isolates obtained from countries other than the U.S. This study applied whole genome sequencing and cgMLST-based typing to evaluate the population structure and genetic relatedness among S. suis isolates obtained within the U.S. The established high-resolution phylogenomic framework revealed extensive genomic variation and diversity among the sampled S. suis isolates, with isolates from the U.S. and from countries outside the U.S. found interspersed in the phylogeny. S. suis isolates obtained within the U.S. did not cluster by state or geographic location, however, isolates with similar serotypes, both obtained from within and outside the U.S., generally clustered together. Average nucleotide identity (ANI) values determined for the S. suis genomes were extensively broad, approaching the recommended species demarcation value, and correlated with the phylogenetic group distribution of the cgMLST-based tree. Numerous antimicrobial resistance (AMR) elements were identified among both U.S. and non-U.S. isolates with ble, tetO, and ermB genes identified as the most prevalent. The epf, mrp, and sly genes, historically used as markers for virulence potential, were also observed in the genomes of isolates that grouped together forming a subclade of clonal complex 1 (CC1) isolates. Collectively, the data in this report provides critical information needed to address potential biosurveillance needs and insights into the genetic diversity and population structure of S. suis isolates obtained within the U.S

Performance of Coupled Product Development Activities with a Deadline

This paper explores the performance of coupled development tasks subject to a deadline constraint by proposing a performance generation model (PGM). The goal of the PGM is to develop insights about optimal strategies (i.e. sequential, concurrent, or overlapped) to manage coupled design tasks that share fixed amount of engineering resources subject to performance and deadline constraints. Model analysis characterizes the solution space for the coupled development problem. The solution space is used to explore the generation of product performance and the associated dynamic forces affecting concurrent development practices. We use these forces to explain conditions under which concurrency is a desirable strategy

Responsible design : recognising the impact of how we design

We live in a period during which human activity is the dominant influence on climate and the environment. Schoolchildren across the world take to the streets calling on all of us –individuals, businesses, and governments – to act responsibly for the future of humanity. University students demand education on doing well by doing good. Member states of the United Nations have committed to the 2030 Agenda for Sustainable Development. Realising sustainable futures inclusive of a good information society certainly sounds attractive to students, business, and governments alike, and we certainly notice heightened awareness and a sense of urgency to tackle large-scale and interconnected challenges such as clean energy, global health, and well-being. Yet, the focus on complex, global challenges has perhaps made it more difficult for individual designers, engineers, and entrepreneurs to feel that their own actions can have a direct impact. Having said this, we also notice that many individuals, businesses, and governments are beginning to ask how might we foresee the impact of the physical and digital products, services, experiences and systems solutions we create. The term "responsible design" is now gaining traction. Still, we can all think of examples of 'successful design' that is harmful to people, damaging to the environment, or bad for our society. We need to recognise our responsibility to change this trajectory, learn from examples of responsible design, and accept the responsibility to make the necessary changes in our own design practice, to achieve system-level change, creating solutions that are regenerative in nature. Many of us are familiar with the 3-point design challenge — desirability, feasibility, and viability. These terms conveniently map to three critical disciplines closely associated with successful design — market, technical, and business. We believe that responsible design is now critical for successful design and thus needs to be part of our community’s discussion, curriculum, and practice. To achieve this goal, we must articulate what responsible design might mean and how we can act accordingly. We offer a definition of responsible design that focuses on responsible behaviour, environmental responsibility, and social responsibility. We illustrate these principles using examples drawn from energy, health care, food, automobile, consumer electronics, and other domains. We propose responsible design as a collective journey that requires the actions of individual designers and design teams. This transition will also affect the nature of innovation processes and the structure of organisations where they take place. The practice of responsible design offers the possibility to engage generations of designers, engineers, entrepreneurs, business, and policy makers to make a difference in our own society through our future products, services, and system solutions. Finally, based on a survey conducted in the lead-up to the conference, in this keynote, we provide a glimpse into how members of the worldwide Design Society are working with the Sustainable Development Goals in research, teaching, and societal outreach

Coordination Implications of Software Coupling in Open Source Projects

The effect of software coupling on the quality of software has been studied quite widely since the seminal paper on software modularity by Parnas [1]. However, the effect of the increase in software coupling on the coordination of the developers has not been researched as much. In commercial software development environments there normally are coordination mechanisms in place to manage the coordination requirements due to software dependencies. But, in the case of Open Source software such coordination mechanisms are harder to implement, as the developers tend to rely solely on electronic means of communication. Hence, an understanding of the changing coordination requirements is essential to the management of an Open Source project. In this paper we study the effect of changes in software coupling on the coordination requirements in a case study of a popular Open Source project called JBoss

Monitoring of lung edema by microwave reflectometry during lung ischemia-reperfusion injury in vivo

It is still unclear whether lung edema can be monitored by microwave reflectometry and whether the measured changes in lung dry matter content (DMC) are accompanied by changes in PaO(2) and in pro-to anti-inflammatory cytokine expression (IFN-gamma and IL-10). Right rat lung hili were cross-clamped at 37 degrees C for 0, 60, 90 or 120 min ischemia followed by 120 min reperfusion. After 90 min (DMC: 15.9 +/- 1.4%; PaO(2): 76.7 +/- 18 mm Hg) and 120 min ischemia (DMC: 12.8 +/- 0.6%; PaO(2): 43 +/- 7 mm Hg), a significant decrease in DMC and PaO(2) throughout reperfusion compared to 0 min ischemia (DMC: 19.5 +/- 1.11%; PaO(2): 247 +/- 33 mm Hg; p < 0.05) was observed. DMC and PaO(2) decreased after 60 min ischemia but recovered during reperfusion (DMC: 18.5 +/- 2.4%; PaO(2) : 173 +/- 30 mm Hg). DMC values reflected changes on the physiological and molecular level. In conclusion, lung edema monitoring by microwave reflectometry might become a tool for the thoracic surgeon. Copyright (c) 2006 S. Karger AG, Basel

Observational reinforcement learning in children and young adults

Observational learning is essential for the acquisition of new behavior in educational practices and daily life and serves as an important mechanism for human cognitive and social-emotional development. However, we know little about its underlying neurocomputational mechanisms from a developmental perspective. In this study we used model-based fMRI to investigate differences in observational learning and individual learning between children and younger adults. Prediction errors (PE), the difference between experienced and predicted outcomes, related positively to striatal and ventral medial prefrontal cortex activation during individual learning and showed no age-related differences. PE-related activation during observational learning was more pronounced when outcomes were worse than predicted. Particularly, negative PE-coding in the dorsal medial prefrontal cortex was stronger in adults compared to children and was associated with improved observational learning in children and adults. The current findings pave the way to better understand observational learning challenges across development and educational settings

Exploring the interplay between intellectual property models and sustainability transitions: A multi‐level analysis

This is the author accepted manuscript. The final version is available from Wiley via the DOI in this recordResearch on international technology transfer and partnership agreements provides a comprehensive understanding of country-level impacts of intellectual property (IP) rights on sustainability transitions. However, firm-level studies on how firms use and share their IP to support sustainability practices remains limited. The paper disentangles the relationship between firm-level IP models and sustainability practices drawing from a cross-case analysis of 28 firms offering sustainable innovations across four sectors. Analysis of firms' year-wise data collected from 854 documents (typically 1996–2021) and 58 in-depth interviews exploring linkage between IP models and sustainability practices of firms engaged in sustainable innovation provide six key findings: (a) emphasis on safeguarding registered and unregistered IP assets among firms with sustainable innovations; (b) widespread adoption of selectively open inbound IP models coupled with diverse IP sharing mechanisms; (c) a preference for collaborative (joint) IP ownership among internally driven firms, contrasting with a tendency for exclusive in-licensing among those reacting to external pressures; (d) a divergence in outbound IP models, with internally motivated firms favouring selectively open approaches and externally driven firms favouring closed IP models; (e) the adoption of fully open outbound IP models democratize sustainable innovation diffusion; and (f) leveraging broadly open outbound IP models alongside closed or selectively open models balances widespread use with access control and achieves significant social sustainability. A framework is hence developed to guide technology-sharing policies and procedures. Therefore, the paper creates a platform for prescribing sustainable IP incentives for encouraging firms to share IP for wider diffusion of sustainable innovations.Belmont ForumNew Opportunities for Research Funding Agency Cooperation in Europe (NORFACE)Bundesministerium für Bildung und ForschungDeutsches Zentrum für Luft- und RaumfahrtGlobal Challenges Research Fund (GCRF)Economic and Social Research Council (ESRC)European Union Horizon 202

Pathogenomes and virulence profiles of representative big six non-O157 serogroup Shiga toxin-producing Escherichia coli

Shiga toxin (Stx)-producing Escherichia coli (STEC) of non-O157:H7 serotypes are responsible for global and widespread human food-borne disease. Among these serogroups, O26, O45, O103, O111, O121, and O145 account for the majority of clinical infections and are colloquially referred to as the “Big Six.” The “Big Six” strain panel we sequenced and analyzed in this study are reference type cultures comprised of six strains representing each of the non-O157 STEC serogroups curated and distributed by the American Type Culture Collection (ATCC) as a resource to the research community under panel number ATCC MP-9. The application of long- and short-read hybrid sequencing yielded closed chromosomes and a total of 14 plasmids of diverse functions. Through high-resolution comparative phylogenomics, we cataloged the shared and strain-specific virulence and resistance gene content and established the close relationship of serogroup O26 and O103 strains featuring flagellar H-type 11. Virulence phenotyping revealed statistically significant differences in the Stx-production capabilities that we found to be correlated to the strain’s individual stx-status. Among the carried Stx1a, Stx2a, and Stx2d phages, the Stx2a phage is by far the most responsive upon RecA-mediated phage mobilization, and in consequence, stx2a + isolates produced the highest-level of toxin in this panel. The availability of high-quality closed genomes for this “Big Six” reference set, including carried plasmids, along with the recorded genomic virulence profiles and Stx-production phenotypes will provide a valuable foundation to further explore the plasticity in evolutionary trajectories in these emerging non-O157 STEC lineages, which are major culprits of human food-borne disease

Extension of internationalisation models: drivers and processes for the globalisation of product development – a comparison of Danish and Chinese engineering firms

This paper develops an extension to established production- and supply chain management focused internationalisation models. It applies explorative case studies in Danish and Chinese engineering firms to discover how the globalisation process of product development differs from Danish and Chinese perspectives. The paper uses internationalisation and global product development theory to explain similarities and differences in the approaches. Grounded in case study results, a new model for internationalisation is proposed. The new model expands the internationalisation process model to include steps of product development and collaborative distributed development beyond sourcing, sales and production elements. The paper then provides propositions for how to further develop the suggested model, and how western companies can learn from the Chinese approaches, and globalise their product development activities from the front end of the value chain rather than from the back-end