Antitumor activity of the novel pyridine derivative

The study aim was to explore a toxicological property and antitumor action of the novel pyridine derivative LHT-17-19 in cell culture and on experimental models of lung cancer in mic

Метанефральная аденома почки: клинический случай и обзор литературы

Metanephric adenoma is a rare benign kidney tumor with a favorable prognosis. These neoplasms are often interpreted as malignant at the preoperative stage, since they do not have specific characteristics showing during instrumental imaging methods, which leads to excessive surgical treatment in the context of nephrectomy volume. Therefore, histological verification is the gold standard for diagnosis. The morphological features of metanephric adenoma are in many ways similar to such kidney tumors as papillary renal cell carcinoma and nephroblastoma (Wilms’ tumor), so sometimes additional research methods are required, including immunohistochemical and molecular genetic ones. In this article, we report a rare clinical case of metanephric adenoma in a forty-seven-year-old woman. The results of clinical and instrumental exams, as well as morphological features of the tumor are presented. A review  of the literature on the clinical, morphological, and molecular aspects of this tumor is also presented.Метанефральная аденома – редкая доброкачественная опухоль почки, характеризующаяся благоприятным прогнозом. Эти новообразования часто интерпретируются на дооперационном этапе как злокачественные, так как не имеют специфических признаков по данным инструментальных методов визуализации, что приводит к избыточному оперативному лечению в объеме нефрэктомии. Следовательно, гистологическая верификация является «золотым стандартом» диагностики. Морфологическая картина метанефральной аденомы во многом схожа с такими опухолями почки, как папиллярный почечно-клеточный рак и нефробластома (опухоль Вильмса), поэтому иногда требуется проведение дополнительных методов исследования, включая иммуногистохимические и молекулярно-генетические. В данной статье мы сообщаем о редком клиническом случае метанефральной аденомы у женщины 47 лет. Представлены результаты клинико-инструментального исследования, морфологические особенности опухоли. Также представлен обзор литературы, посвященной клиническим, морфологическим и молекулярным аспектам данной опухоли

SYSTEMATIZATION AND ANALYSIS OF PARTIALLY AND FULLY HOMOMORPHIC CRYPTOSYSTEM

In this article provides an overview of the known partially and fully homomorphic cryptosystem, such as: RSA, ElGamal, Paillier, Gentry and Halevi. Justified the homomorphic properties of the considered cryptosystems. The comparative analysis of the homomorphic encryption algorithms has been committe

The Combination of In Vitro Assessment of Stress Tolerance Ability, Autoaggregation, and Vitamin B-Producing Ability for New Probiotic Strain Introduction

The health benefits of probiotics are beyond doubt. The positive effects of lactobacilli and bifidobacteria on the function of many body systems have been repeatedly proven by various studies. To completely realize the potential of probiotic microorganisms, the strains should be tested by the greatest combination of characteristics that contribute to the wellness of the host. In this work, for the first time, a combined assessment of the probiotic properties and vitamin B-producing potential of various species and strains of bifidobacteria and lactobacilli was carried out. The presence of an additional advantage, such as vitamin-producing ability, can prevent vitamin deficiency both at the level of the consumption of fermented foods, when the enrichment will occur naturally on the spot, and during colonization by these intestinal strains, when synthesis will occur in vivo. To select potential probiotics, the stress tolerance ability of 16 lactic acid bacteria and bifidobacteria strains to low pH values, bile, and proteolytic enzymes, as well as their ability to autoaggregate, were studied under conditions of modeling the gastrointestinal tract in vitro. The ability of the strains to extracellularly accumulate water-soluble B vitamins was evaluated by capillary electrophoresis. Among the tested strains of bifidobacteria, B. adolescentis VKPM AC-1662 is of interest; it was characterized by the greatest stress tolerance ability and the ability to autoaggregate, in addition to the extracellular synthesis of riboflavin and pyridoxine. Among lactic acid bacteria, L. sakei VKPM B-8936 demonstrated the greatest tolerance to low pH, L. plantarum VKPM B–11007 to duodenal conditions, L. acidophilus VKPM B-2213 to pepsin, and L. salivarius VKPM B–2214 to pancreatin. The highest percentage of autoaggregation was observed in L. salivarius VKPM B-2214, which also accumulated the largest amount of pantothenic acid, but it was sensitive to stress conditions. The obtained results could be used to create new products enriched with probiotics and B vitamins

A thermophilic L-lactic acid producer of high optical purity: isolation and identification

Biodegradable polymers, specifically polylactide, are an important part of food packaging and medical devices. Microbiological synthesis uses cheap renewable raw materials and industrial waste to produce a high yield of lactic acid, the monomer of polylactide. This method needs new effective lactic acid producing strains, e.g., thermophilic bacteria. The research involved thermophilic bacterial strains isolated from soil and compost samples. Their ability to produce organic acids and extracellular enzymes was tested using the method of high-performance liquid chromatography (HPLC) and microbiological tests respectively. The real-time polymerase chain reaction method (PCR) detected L-lactate dehydrogenase structural genes of L-lactate dehydrogenase of Bacillaceae. Strain T7.1 was fermented using glucose and yeast extract as carbon and nitrogen sources, respectively. The optical purity of lactic acid was evaluated using quantitative gas chromatography on a chiral column to separate lactate isomers. The molecular genetic analysis of the 16S rRNA gene sequence was applied to identify strain T7.1. The chromatographic analysis proved that 10 out of 13 isolated thermophilic strains were effective lactic acid producers. They demonstrated proteolytic, amylolytic, or cellulase activities. During the fermentation, strain T7.1 produced 81 g/L of lactic acid with a peak productivity at 1.58 g/(L·h). The optical purity of the product exceeded 99.9% L-lactate. The genetic analysis identified strain T7.1 as Weizmannia coagulans (Bacillus coagulans). The research revealed a promising thermophilic producer of optically pure L-lactic acid. Further research is needed to optimize the cultivation conditions, design an effective and cheap nutrient medium, and develop engineering and technological solutions to increase the yield

Expression of p53 protein associates with anti-pd-l1 treatment response on human-derived xenograft model of gata3/cr5/6-negative recurrent nonmuscular invasive bladder urothelial carcinoma

Background: The possible involvement of p53 signaling, FGFR3 expression, and FGFR3 mutation rates in the prediction of the NMIBC anti-PD-L1 treatment response needs to be clarified. The main aim of our study was to explore predictive value of p53 expression, FGFR3 expression, and its gene mutation status for the therapeutic success of anti-PD-L1 treatment in the patient-derived murine model of recurrent high-PD-L1(+) GATA3(-)/CR5/6(-) high-grade and low-grade NMIBC. Methods: twenty lines of patient-derived xenografts (PDXs) of relapsed high-PD-L1(+) double-negative NMIBC were developed, of which 10 lines represented high-grade tumors and the other ones—low-grade bladder cancer. Acceptors of each grade-related branch received specific anti-PD-L1 antibodies. Animals’ survival, tumor-doubling time, and remote metastasis were followed during the post-interventional period. PD-L1, GATA3, CR5/6, and p53 protein expressions in engrafted tumors were assessed by immunohistochemistry. The FGFR3 expression and FGFR3 mutations in codons 248 and 249 were detected by real-time polymerase chain reaction. Results: The expression of p53 protein is an independent factor affecting the animals’ survival time [HR = 0.036, p = 0.031] of anti-PD-L1-treated mice with low-grade high-PD-L1(+) double-negative NMIBC PDX. The FGFR3 expression and FGFR3 mutation rate have no impact on the anti-PD-L1 treatment response in the interventional groups. Conclusions: p53 expression may be considered as a prognostic factor for the anti-PD-L1 treatment efficacy of low-grade high-PD-L1-positive GATA3(-)/CR5/6(-)relapsed noninvasive bladder cancer. © 2021 by the authors. Licensee MDPI, Basel, Switzerland