20 research outputs found
A labeled spectral dataset with cassava disease occurrences using virus titre determination protocol
In this work, we present a novel dataset composed of spectral data and images of cassava crops with and without diseases. Together with the description of the dataset, we describe the protocol to collect such data in a controlled environment and in an open field where pests are not controlled. Crop disease diagnosis has been done in the past through the analysis of plant images taken with a smartphone camera. However, in some cases, disease symptoms are not visible. Furthermore, for some cassava diseases, once symptoms have manifested on the aerial part of the plant, the root which is the edible part of the plant has been totally destroyed. The goal of collecting this multimodality of the crop disease is early intervention, following the hypothesis that diseased crops without visible symptoms can be detected using spectral information. We collected visible and near-infrared spectra captured from leaves infected with two common cassava diseases namely; Cassava Brown Streak Disease and Cassava Mosaic Disease, as well as from healthy plants. Together, we also captured leaf imagery data that corresponds to the spectral information. In our experiments, biochemical data is collected and taken as the ground truth. Finally, agricultural experts provided a disease score per plant leaf from 1 to 5, 1 representing healthy and 5 severely diseased. The process of disease monitoring and data collection took 19 and 15 consecutive weeks for screenhouse and open field, respectively, until disease symptoms were visibly seen by the human eye.</p
Phenotype based clustering, and diversity of common bean genotypes in seed iron concentration and cooking time
Common bean is the worldâs most important directly consumed legume food crop that is popular for calories, protein and micronutrients. It is a staple food in sub-Saharan Africa, and a significant source of iron for anemic people. However, several pests, soil and weather challenges still impede its production. Long cooking time, and high phytic acid and polyphenols that influence bioavailable iron also limit the health benefits. To inform population improvement strategies and selection decisions for resilient fast cooking and iron biofortified beans, the study determined diversity and population structure within 427 breeding lines, varieties, or landraces mostly from Alliance Uganda and Columbia. The genotypes were evaluated for days to flowering and physiological maturity, yield, seed iron (FESEED) and zinc (ZNSEED) and cooking time (COOKT). Data for all traits showed significant (Pâ€0.001) differences among the genotypes. Repeatability was moderate to high for most traits. Performance ranged from 52 to 87 ppm (FESEED), 23â38 ppm (ZNSEED), 36â361 minutes (COOKT), and 397â1299 kg/ha (yield). Minimal differences existed between the gene pools in the mean performance except in yield, where Mesoamerican beans were better by 117 kg/ha. The genotypes exhibited high genetic diversity and thus have a high potential for use in plant breeding. Improvement of FESEED and ZNSEED, COOKT and yield performance within some markets such as red and small white beans is possible. Hybridization across market classes especially for yellow beans is essential but this could be avoided by adding other elite lines to the population. Superior yielding and fast cooking, yellow and large white beans were specifically lacking. Adding Fe dense elite lines to the population is also recommended. The population was clustered into three groups that could be considered for specific breeding targets based on trait correlations
Near-infrared spectroscopy applications for high-throughput phenotyping for cassava and yam: a review
Open Access Article; Published online: 12 Aug 2020The review aimed to identify the different highâthroughput phenotyping (HTP) techniques that used for quality evaluation in cassava and yam breeding programmes, and this has provided insights towards the development of metrics and their application in cassava and yam improvements. A systematic review of the published research articles involved the use of NIRS in analysing the quality traits of cassava and yam was carried out, and Scopus, Science Direct, Web of Sciences and Google Scholar were searched. The results of the review established that NIRS could be used in understanding the chemical constituents (carbohydrate, protein, vitamins, minerals, carotenoids, moisture, starch, etc.) for highâthroughput phenotyping. This study provides preliminary evidence of the application of NIRS as an efficient and affordable procedure for HTP. However, the feasibility of using midâinfrared spectroscopy (MIRS) and hyperspectral imaging (HSI) in combination with the NIRS could be further studied for quality traits phenotyping
Crystalline and pasting properties of cassava starch are influenced by its molecular properties
This study was undertaken to detail starch characteristics among cassava varieties and compare them to their improved progenies. Cassava starch was extracted from roots of both the parents and progenies of two popular Ugandan local varieties (Bamunanika and Nyaraboke) and three popular elite varieties (NASE 10, TME 14 and 95/SE/00036) and their properties compared. The pasting and rheological properties showed a unique pasting curve in the progenies compared to the parents with significantly low peak viscosities among the progenies. Percentage crystallinity as determined by X-ray
crystallography was on average four points higher in parents compared to progenies. There were no
significant differences in the average amylose contents (17 - 20%) and starch contents (about 81%) in both the progenies and their parents. Significant relationships were observed between crystalline and pasting properties of the starch among the clones and parents. The above differences suggest possible variations in the amylopectin chain structure and starch molecular properties attributable to differences in the starch branching enzyme among the progenies and their parents.This work was supported under the BIO-EARN program,
with a grant from the Swedish International Cooperation
Agency/Department for Research Cooperation (Sida/
SAREC)
Physicochemical and functional characteristics of cassava starch in Ugandan varieties and their progenies
Due to poor root quality traits in improved, disease resistant cassava (Manihot esculenta Crantz.)
varieties and hence low acceptability among farmers, a study was undertaken to improve these
varieties by crossing them with disease susceptible, farmer preferred local landraces. Five improved
varieties and four local landraces were used and hybridisations among them were made in a poly-cross
nursery block. Starch isolated from the nine cassava varieties and their F1 progenies was analysed for
physicochemical and functional properties. Significant differences were observed between varieties,
progenies and within the F1 progenies. The amylose content ranging between 19.0 - 25.0% was
negatively correlated to swelling power and solubility but positively correlated to starch content.
Average starch granule sizes ranged between 7.0 - 12.0 mm, though smaller granules ranged between 2
- 6.9 mm and large granules between 13 â 20 mm. Granules were mainly truncated in shape and similar
across varieties and their progenies. Individual parents had peak viscosity, set back viscosity and
viscosity at breakdown higher than the progenies suggesting inherent genetic and biochemical
differences among parents used in the study. Variations were also observed in the parents and
progenies for starch swelling power, solubility and starch content on dry basis. Starch associated
molecules such as proteins and lipids did not vary significantly but dietary fibre significantly (P< 0.05)
varied both in parents and F1 families. Significant correlations (r > 0.45) were observed among starch
properties including swelling power and breakdown viscosity. Based on these results, selections for
lines with different starch quality and quantity properties can be made among the F1 families for future
dietary and industrial uses
Starch quality traits of improved provitamin A cassava (Manihot esculenta Crantz)
Provitamin A cassava clones were analysed for starch yield and critical starch quality attributes, to understand possible applications in the food industry. Total carotenoids content in the test clones ranged from 0.03-11.94 ÎŒg g-1 of fresh root. Starch yield ranged from 8.4-33.2 % and correlated negatively (r = -0.588, P < 0.001) with carotenoids content. Amylose content (16.4â22.1%) didn't differ significantly (P †0.05) among the cassava clones. Meanwhile, total carotenoid content had significant negative correlations (P †0.05) with starch pasting temperature, peak time, setback viscosities and peak area. The reduced peak time and pasting temperatures in high-carotenoid cassava signifies reduction in energy requirements in yellow-fleshed roots when compared to white-fleshed cassava. This attribute is desirable for the food industry as it would reduce the overall cost of processing the cassava. Furthermore, final viscosities of starch from carotenoid-rich cassava were lower than those of white-fleshed roots, making provitamin A cassava suitable for soft food processing
Early detection of plant diseases using spectral data
Early detection of crop disease is an essential step in food security. Usually, the detection becomes possible in a stage where disease symptoms are already visible on the aerial part of the plant. However, once the disease has manifested in different parts of the plant, little can be done to salvage the situation. Here, we suggest that the use of visible and near infrared spectral information facilitates disease detection in cassava crops before symptoms can be seen by the human eye. To test this hypothesis, we grow cassava plants in a screen house where they are inoculated with disease viruses. We monitor the plants over time collecting both spectra and plant tissue for wet chemistry analysis. Our results demonstrate that suitably trained classifiers are indeed able to detect cassava diseases. Specifically, we consider Generalized Matrix Relevance Learning Vector Quantization (GMLVQ) applied to original spectra and, alternatively, in combination with dimension reduction by Principal Component Analysis (PCA). We show that successful detection is possible shortly after the infection can be confirmed by wet lab chemistry, several weeks before symptoms manifest on the plants
Volatile organic compound based markers for the aroma trait of rice grain
A study was conducted to determine the volatile organic compounds (VOCs) associated with rice grain aroma in 37 commonly grown lines within Uganda, as well as elites. The aim of the study was to identify potential volatile biochemical markers, if any, for the rice grain aroma trait. Certified rice seeds were obtained from the Uganda National Crops Resources Research Institute germplasm collection. The seeds were sown into experimental plots, under field conditions and the mature paddy harvested. Polished rice grains were heated to 80 oC and the liberated VOCs subjected to untargeted metabolite analysis using gas chromatography-time-of-flight mass spectrometry. In total, nine functional groups were present; hydrocarbons, alcohols, ketones, aldehydes, N-containing compounds, S-containing compounds, esters, oxygen heterocycles and carboxylic acids. More specifically, 148 VOCs were identified across the 37 rice lines, of which 48 (32.4%) including 2-acetyl-1-pyrroline (2-AP) appeared to elucidate the difference between non-aromatic and aromatic rice. Furthermore, 41 (27.7%) VOCs were found to be significantly correlated with 2-AP abundance, the principle rice aroma compound. Amongst the 41 VOCs, only ten compounds were found to contribute highly towards variation in 2-AP abundance, indicative of their possible modulation roles in regard to rice aroma. Within the ten influential volatiles, three aroma active compounds; toluene, 1-hexanol, 2-ethyl and heptane, 2,2,4,6,6-pentamethyl- were established as the most reliable biochemical surrogates to the rice aroma trait. Thus, the aforementioned compounds may be used in rice breeding programme for enhancing development of the grain aroma trait