481 research outputs found
Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia
BACKGROUND: Adeno-associated virus type 2 (AAV-2) attachment and internalization is thought to be mediated by host cell membrane-associated heparan sulfate proteoglycans (HSPG). Lack of HSPG on the apical membrane of respiratory epithelial cells has been identified as a reason for inefficient rAAV-2 infection in pulmonary applications in-vivo. The aim of this investigation was to determine the necessity of cell membrane HSPG for efficient infection by rAAV-2. RESULTS: Rates of transduction with rAAV2-CMV-EGFP3 in several different immortalized airway epithelial cell lines were determined at different multiplicities of infection (MOI) before and after removal of membrane HSPG by heparinase III. Removal of HSPG decreased the efficacy of infection with rAAV2 by only 30â35% at MOI †100 for all of respiratory cell lines tested, and had even less effect at an MOI of 1000. Studies in mutant Chinese Hamster Ovary cell lines known to be completely deficient in surface HSPG also demonstrated only moderate effect of absence of HSPG on rAAV-2 infection efficacy. However, mutant CHO cells lacking all membrane proteoglycans demonstrated dramatic reduction in susceptibility to rAAV-2 infection, suggesting a role of membrane glycosaminoglycans other than HSPG in mediating rAAV-2 infection. CONCLUSION: Lack of cell membrane HSPG in pulmonary epithelia and other cell lines results in only moderate decrease in susceptibility to rAAV-2 infection, and this decrease may be less important at high MOIs. Other cell membrane glycosaminoglycans can play a role in permitting attachment and subsequent rAAV-2 internalization. Targeting alternative membrane glycosaminoglycans may aid in improving the efficacy of rAAV-2 for pulmonary applications
Parton Energy Loss with Detailed Balance
Stimulated gluon emission and thermal absorption in addition to induced
radiation are considered for an energetic parton propagating inside a quark-
gluon plasma. In the presence of thermal gluons, stimulated emission reduces
while absorption increases the parton's energy. The net effect is a reduction
of the parton energy loss. Though decreasing asymptotically as with the
parton energy, the relative reduction is found to be important for intermediate
energies. The modified energy dependence of the energy loss will affect the
shape of suppression of moderately high hadrons due to jet quenching in
high-energy heavy-ion collisions.Comment: 4 pages in RevTex with 1 postscript figure. Some typos are corrected
and new comments are adde
A study on the anomaly of over ratios in collisions with jet quenching
The ratios of at large transverse momentum in central
collisions at RHIC are studied in the framework of jet quenching based on a
next-to-leading order pQCD parton model. It is shown that theoretical
calculations with a gluon energy loss larger than the quark energy loss will
naturally lead to a smaller ratios at large transverse momentum in
collisions than those in collisions at the same energy. Scenarios
with equal energy losses for gluons and quarks and a strong jet conversion are
both explored and it is demonstrated in both scenarios ratios at high
in central collisions are enhanced and the calculated ratios of
protons over pions approach to the experimental measurements. However, in the latter scenario is found to fit data better than that in the
former scenario.Comment: 20 pages, 13 figures; revised version; accepted for publication in
Journal of Physics
The MUSE-Wide Survey: Survey Description and First Data Release
We present the MUSE-Wide survey, a blind, 3D spectroscopic survey in the
CANDELS/GOODS-S and CANDELS/COSMOS regions. Each MUSE-Wide pointing has a depth
of 1 hour and hence targets more extreme and more luminous objects over 10
times the area of the MUSE-Deep fields (Bacon et al. 2017). The legacy value of
MUSE-Wide lies in providing "spectroscopy of everything" without photometric
pre-selection. We describe the data reduction, post-processing and PSF
characterization of the first 44 CANDELS/GOODS-S MUSE-Wide pointings released
with this publication. Using a 3D matched filtering approach we detected 1,602
emission line sources, including 479 Lyman- (Lya) emitting galaxies
with redshifts . We cross-match the emission line
sources to existing photometric catalogs, finding almost complete agreement in
redshifts and stellar masses for our low redshift (z < 1.5) emitters. At high
redshift, we only find ~55% matches to photometric catalogs. We encounter a
higher outlier rate and a systematic offset of z0.2 when
comparing our MUSE redshifts with photometric redshifts. Cross-matching the
emission line sources with X-ray catalogs from the Chandra Deep Field South, we
find 127 matches, including 10 objects with no prior spectroscopic
identification. Stacking X-ray images centered on our Lya emitters yielded no
signal; the Lya population is not dominated by even low luminosity AGN. A total
of 9,205 photometrically selected objects from the CANDELS survey lie in the
MUSE-Wide footprint, which we provide optimally extracted 1D spectra of. We are
able to determine the spectroscopic redshift of 98% of 772 photometrically
selected galaxies brighter than 24th F775W magnitude. All the data in the first
data release - datacubes, catalogs, extracted spectra, maps - are available on
the website https://musewide.aip.de. [abridged]Comment: 25 pages 15+1 figures. Accepted, A&A. Comments welcom
Photo-astrometric distances, extinctions, and astrophysical parameters for Gaia DR2 stars brighter than G = 18
Combining the precise parallaxes and optical photometry delivered by Gaia's
second data release (Gaia DR2) with the photometric catalogues of PanSTARRS-1,
2MASS, and AllWISE, we derive Bayesian stellar parameters, distances, and
extinctions for 265 million stars brighter than G=18. Because of the wide
wavelength range used, our results substantially improve the accuracy and
precision of previous extinction and effective temperature estimates. After
cleaning our results for both unreliable input and output data, we retain 137
million stars, for which we achieve a median precision of 5% in distance, 0.20
mag in V-band extinction, and 245 K in effective temperature for G<14,
degrading towards fainter magnitudes (12%, 0.20 mag, and 245 K at G=16; 16%,
0.23 mag, and 260 K at G=17, respectively). We find a very good agreement with
the asteroseismic surface gravities and distances of 7000 stars in the Kepler,
the K2-C3, and the K2-C6 fields, with stellar parameters from the APOGEE
survey, as well as with distances to star clusters. Our results are available
through the ADQL query interface of the Gaia mirror at the Leibniz-Institut
f\"{u}r Astrophysik Potsdam (gaia.aip.de) and as binary tables at data.aip.de.
As a first application, in this paper we provide distance- and
extinction-corrected colour-magnitude diagrams, extinction maps as a function
of distance, and extensive density maps, demonstrating the potential of our
value-added dataset for mapping the three-dimensional structure of our Galaxy.
In particular, we see a clear manifestation of the Galactic bar in the stellar
density distributions, an observation that can almost be considered a direct
imaging of the Galactic bar.Comment: 25 pages, 23 figures + appendix, accepted for publication in A&A.
Data (doi:10.17876/gaia/dr.2/51) are available through ADQL queries at
gaia.aip.d
Pathway to the Square Kilometre Array - The German White Paper -
The Square Kilometre Array (SKA) is the most ambitious radio telescope ever
planned. With a collecting area of about a square kilometre, the SKA will be
far superior in sensitivity and observing speed to all current radio
facilities. The scientific capability promised by the SKA and its technological
challenges provide an ideal base for interdisciplinary research, technology
transfer, and collaboration between universities, research centres and
industry. The SKA in the radio regime and the European Extreme Large Telescope
(E-ELT) in the optical band are on the roadmap of the European Strategy Forum
for Research Infrastructures (ESFRI) and have been recognised as the essential
facilities for European research in astronomy.
This "White Paper" outlines the German science and R&D interests in the SKA
project and will provide the basis for future funding applications to secure
German involvement in the Square Kilometre Array.Comment: Editors: H. R. Kl\"ockner, M. Kramer, H. Falcke, D.J. Schwarz, A.
Eckart, G. Kauffmann, A. Zensus; 150 pages (low resolution- and colour-scale
images), published in July 2012, language English (including a foreword and
an executive summary in German), the original file is available via the MPIfR
homepag
Cold Nuclear Matter Effects on Dijet Productions in Relativistic Heavy-ion Reactions at LHC
We investigate the cold nuclear matter(CNM) effects on dijet productions in
high-energy nuclear collisions at LHC with the next-to-leading order
perturbative QCD. The nuclear modifications for dijet angular distributions,
dijet invariant mass spectra, dijet transverse momentum spectra and dijet
momentum imbalance due to CNM effects are calculated by incorporating EPS, EKS,
HKN and DS param-etrization sets of parton distributions in nucleus . It is
found that dijet angular distributions and dijet momentum imbalance are
insensitive to the initial-state CNM effects and thus provide optimal tools to
study the final-state hot QGP effects such as jet quenching. On the other hand,
the invariant mass spectra and the transverse momentum spectra of dijet are
generally enhanced in a wide region of the invariant mass or transverse
momentum due to CNM effects with a feature opposite to the expected suppression
because of the final-state parton energy loss effect in the QGP. The difference
of EPS, EKS, HKN and DS parametrization sets of nuclear parton distribution
functions is appreciable for dijet invariant mass spectra and transverse
momentum spectra at p+Pb collisions, and becomes more pronounced for those at
Pb+Pb reactions.Comment: 10 pages, 11 figure
A Generalized Fluctuation-Dissipation Theorem for Nonlinear Response Functions
A nonlinear generalization of the Fluctuation-Dissipation Theorem (FDT) for
the n-point Green functions and the amputated 1PI vertex functions at finite
temperature is derived in the framework of the Closed Time Path formalism. We
verify that this generalized FDT coincides with known results for n=2 and 3.
New explicit relations among the 4-point nonlinear response and correlation
(fluctuation) functions are presented.Comment: 34 pages, Revte
Spallation Neutron Production by 0.8, 1.2 and 1.6 GeV Protons on various Targets
Spallation neutron production in proton induced reactions on Al, Fe, Zr, W,
Pb and Th targets at 1.2 GeV and on Fe and Pb at 0.8, and 1.6 GeV measured at
the SATURNE accelerator in Saclay is reported. The experimental
double-differential cross-sections are compared with calculations performed
with different intra-nuclear cascade models implemented in high energy
transport codes. The broad angular coverage also allowed the determination of
average neutron multiplicities above 2 MeV. Deficiencies in some of the models
commonly used for applications are pointed out.Comment: 20 pages, 32 figures, revised version, accepted fpr publication in
Phys. Rev.
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