Expression site attenuation mechanistically links antigenic variation and development in Trypanosoma brucei

We have discovered a new mechanism of monoallelic gene expression that links antigenic variation, cell cycle, and development in the model parasite Trypanosoma brucei. African trypanosomes possess hundreds of variant surface glycoprotein (VSG) genes, but only one is expressed from a telomeric expression site (ES) at any given time. We found that the expression of a second VSG alone is sufficient to silence the active VSG gene and directionally attenuate the ES by disruptor of telomeric silencing-1B (DOT1B)-mediated histone methylation. Three conserved expression-site-associated genes (ESAGs) appear to serve as signal for ES attenuation. Their depletion causes G1-phase dormancy and reversible initiation of the slender-to-stumpy differentiation pathway. ES-attenuated slender bloodstream trypanosomes gain full developmental competence for transformation to the tsetse fly stage. This surprising connection between antigenic variation and developmental progression provides an unexpected point of attack against the deadly sleeping sickness

Standard Solar models in the Light of New Helioseismic Constraints II. Mixing Below the Convective Zone

In previous work, we have shown that recent updated standard solar models cannot reproduce the radial profile of the sound speed at the base of the convective zone (CZ) and fail to predict the Li7 depletion. In parallel, helioseismology has shown that the transition from differential rotation in the CZ to almost uniform rotation in the radiative solar interior occurs in a shallow layer called the tachocline. This layer is presumably the seat of large scale circulation and of turbulent motions. Here, we introduce a macroscopic transport term in the structure equations, which is based on a hydrodynamical description of the tachocline proposed by Spiegel and Zahn, and we calculate the mixing induced within this layer. We discuss the influence of different parameters that represent the tachocline thickness, the Brunt-Vaissala frequency at the base of the CZ, and the time dependence of this mixing process along the Sun's evolution. We show that the introduction of such a process inhibits the microscopic diffusion by about 25%. Starting from models including a pre-main sequence evolution, we obtain: a) a good agreement with the observed photospheric chemical abundance of light elements such as He3, He4, Li7 and Be9, b) a smooth composition gradient at the base of the CZ, and c) a significant improvement of the sound speed square difference between the seismic sun and the models in this transition region, when we allow the phostospheric heavy element abundance to adjust, within the observational incertitude, due to the action of this mixing process. The impact on neutrino predictions is also discussed.Comment: 15 pages, 7 figures, to be published in ApJ (used emulateapj style for latex2e). New email for A. S. Brun: [email protected]

Screening enhancement factors for laboratory CNO and rp astrophysical reactions

Cross sections of laboratory CNO and rp astrophysical reactions are enhanced due to the presence of the multi-electron cloud that surrounds the target nuclei. As a result the relevant astrophysical factors are overestimated unless corrected appropriately. This study gives both an estimate of the error committed if screening effects are not taken into account and a rough profile of the laboratory energy thresholds at which the screening effect appears. The results indicate that, for most practical purposes, screening corrections to past relevant experiments can be disregarded. Regarding future experiments, however, screening corrections to the CNO reactions will certainly be of importance as they are closely related to the solar neutrino fluxes and the rp process. Moreover, according to the present results, screening effects will have to be taken into account particularly by the current and future LUNA experiments, where screened astrophysical factors will be enhanced to a significant degree.Comment: 6 RevTex pages + 2 ps figures. (Revised version). Accepted for publication in Journal of Physics

Macroscopic Self-Evolution of Dynamic Hydrogels to Create Hollow Interiors

A solid-to-hollow evolution in macroscopic structures is challenging in synthetic materials. A fundamentally new strategy is reported for guiding macroscopic, unidirectional shape evolution of materials without compromising the material's integrity. This strategy is based on the creation of a field with a “swelling pole” and a “shrinking pole” to drive polymers to disassemble, migrate, and resettle in the targeted region. This concept is demonstrated using dynamic hydrogels containing anchored acrylic ligands and hydrophobic long alkyl chains. Adding water molecules and ferric ions (Fe3+) to induce a swelling–shrinking field transforms the hydrogels from solid to hollow. The strategy is versatile in the generation of various closed hollow objects (for example, spheres, helix tubes, and cubes with different diameters) for different applications

Screening of Nuclear Reactions in the Sun and Solar Neutrinos

We quantitatively determine the effect and the uncertainty on solar neutrino production arising from the screening process. We present predictions for the solar neutrino fluxes and signals obtained with different screening models available in the literature and by using our stellar evolution code. We explain these numerical results in terms of simple laws relating the screening factors with the neutrino fluxes. Futhermore we explore a wider range of models for screening, obtained from the Mitler model by introducing and varying two phenomenological parameters, taking into account effects not included in the Mitler prescription. Screening implies, with respect to a no-screening case, a central temperat reduction of 0.5%, a 2% (8%) increase of Beryllium (Boron)-neutrino flux and a 2% (12%) increase of the Gallium (Chlorine) signal. We also find that uncertainties due to the screening effect ar at the level of 1% for the predicted Beryllium-neutrino flux and Gallium signal, not exceeding 3% for the Boron-neutrino flux and the Chlorine signal.Comment: postscript file 11 pages + 4 figures compressed and uuencoded we have replaced the previous paper with a uuencoded file (the text is the same) for any problem please write to [email protected]

Atomic effects in astrophysical nuclear reactions

Two models are presented for the description of the electron screening effects that appear in laboratory nuclear reactions at astrophysical energies. The two-electron screening energy of the first model agrees very well with the recent LUNA experimental result for the break-up reaction $He3(He3,2p)He^{4}$, which so far defies all available theoretical models. Moreover, multi-electron effects that enhance laboratory reactions of the CNO cycle and other advanced nuclear burning stages, are also studied by means of the Thomas-Fermi model, deriving analytical formulae that establish a lower and upper limit for the associated screening energy. The results of the second model, which show a very satisfactory compatibility with the adiabatic approximation ones, are expected to be particularly useful in future experiments for a more accurate determination of the CNO astrophysical factors.Comment: 14 RevTex pages + 2 ps (revised) figures. Phys.Rev.C (in production

Astrophysical factors:Zero energy vs. Most effective energy

Effective astrophysical factors for non-resonant astrophysical nuclear reaction are invariably calculated with respect to a zero energy limit. In the present work that limit is shown to be very disadvantageous compared to the more natural effective energy limit. The latter is used in order to modify the thermonuclear reaction rate formula so that it takes into account both plasma and laboratory screening effects.Comment: 7 RevTex pages. Accepted for publication in Phys.Rev.

Metadata schema and mapping service for FIB/SEM serial-sectioning and computed tomography

Tomographic methods on different length scales using different physical principles are well established tools in Materials Science and Engineering (MSE). One of the challenges from a research data management (RDM) perspective in this field is that the tomography process, i.e. data acquisition, is only one part of the entire experimental workflow of materials characterization, which starts with a sample and ends with a reconstructed 3D model. Each step, from sample preparation to data acquisition to post-processing and 3D reconstruction, takes place in its own ecosystem of hardware and software, and there is little standardization in the MSE community on how to collect and use metadata to describe the workflow. This ultimately hinders the (re-)use of data according to the FAIR principles. In an attempt to improve this situation, we present an excerpt of the ongoing work in Participant Project 13 "Tomography and microstructure-based modeling" of the NFDI-MatWerk consortium. In a collaboration of domain and metadata experts, metadata schemas for the representation of the data acquisition process in FIB/SEM serial sectioning and computed tomography have been developed and implemented using the FAIR Digital Object concept. Furthermore, an automated extraction and mapping service for extracting metadata from existing FIB/SEM tomography datasets and mapping them to the terms of the metadata schema is presented, which provides a one-click solution to collect tomography metadata scattered across different files and write it into a JSON metadata document according to the developed schema. The final result is a FAIR Digital Object pointing to both data and metadata resources. This poster covers both the technical aspects and the researcher\u27s perspective

Radiation correction to astrophysical fusion reactions and the electron screening problem

We discuss the effect of electromagnetic environment on laboratory measurements of the nuclear fusion reactions of astrophysical interest. The radiation field is eliminated using the path integral formalism in order to obtain the influence functional, which we evaluate in the semi-classical approximation. We show that enhancement of the tunneling probability due to the radiation correction is extremely small and does not resolve the longstanding problem that the observed electron screening effect is significantly larger than theoretical predictions.Comment: 9 pages, 1 eps figure

Predicting Quality of Clinical Performance From Cardiology Fellowship Applications

Variables in cardiology fellowship applications have not been objectively analyzed against applicants\u27 subsequent clinical performance. We investigated possible correlations in a retrospective cohort study of 65 cardiology fellows at the Mayo Clinic (Rochester, Minn) who began 2 years of clinical training from July 2007 through July 2013. Application variables included the strength of comparative statements in recommendation letters and the authors\u27 academic ranks, membership status in the Alpha Omega Alpha Honor Medical Society, awards earned, volunteer activities, United States Medical Licensing Examination (USMLE) scores, advanced degrees, publications, and completion of a residency program ranked in the top 6 in the United States. The outcome was clinical performance as measured by a mean of faculty evaluation scores during clinical training. The overall mean evaluation score was 4.07 ± 0.18 (scale, 1-5). After multivariable analysis, evaluation scores were associated with Alpha Omega Alpha designation (β=0.13; 95% CI, 0.01-0.25; P=0.03), residency program reputation (β=0.13; 95% CI, 0.05-0.21; P=0.004), and strength of comparative statements in recommendation letters (β=0.08; 95% CI, 0.01-0.15; P=0.02), particularly in letters from residency program directors (β=0.05; 95% CI, 0.01-0.08; P=0.009). Objective factors to consider in the cardiology fellowship application include Alpha Omega Alpha membership, residency program reputation, and comparative statements from residency program directors