The Queen\u27s Software: A Personal Exploration on Failure, Discovery and a Commitment to Not Knowing

I am interested in how failure, promiscuity, irrelevance and delight can live simultaneously in a process based space. Dancing lives in between knowing exactly what and not knowing at all. Dance is my middle, my way of being even when disjointed, discombobulated and on the brink of failure; dancing enchants my inner freak. I actively try to cultivate a process as a place of exploration, research and investigation with permission to change, evolve and develop into something very different from the initial starting place. How my process can live in between pleasure and function, meaningfulness and abstraction, investigation and direction is relevant in keeping a process complex and stimulating. Searching for what is revealed through process and not what is given to benefit the product is where I seek to depart from dissecting a work for its content. I am interested in work that raises questions, feelings and ponderings without clear answers. I don’t know what I want to make, but that does not mean I can’t find out along the way. It is here that I find vitality, it is here that I begin

Prospectus, April 25, 2019

STUDENT WINS BOSTON MARATHON WHEELCHAIR DIVISION!, Humans of Parkland: Mo Belakhoua, The Notre Dame Fire: A Historical Tragedy, 5 fun things to do in the Spring!, Final Examinations-Spring semester, 2019, Krazy Noodle Massacre, No. 5 Cobras Earn Series Win Against No. 16 Heartlandhttps://spark.parkland.edu/prospectus_2019/1012/thumbnail.jp

Prospectus, April 25, 2019

STUDENT WINS BOSTON MARATHON WHEELCHAIR DIVISION!, Humans of Parkland: Mo Belakhoua, The Notre Dame Fire: A Historical Tragedy, 5 fun things to do in the Spring!, Final Examinations-Spring semester, 2019, Krazy Noodle Massacre, No. 5 Cobras Earn Series Win Against No. 16 Heartlandhttps://spark.parkland.edu/prospectus_2019/1012/thumbnail.jp

Prospectus, April 18, 2019

STUDENT GOVERNMENT PARTICIPATES IN ITS FIRST ADVOCACY DAY, Empathy and Love at Roger Ebert Feat 2019, Parkland Celebrates Earth Day with Sustainability Fair, Work by Our Creative Cobras Showcased, Vet Tech students washing dogs for $10!, Krazy Noodle Massacre, Parkland Golf Takes Second at Small College Championship, Cobras Silence Snappers on Saturday DH Routehttps://spark.parkland.edu/prospectus_2019/1011/thumbnail.jp

BIOTINYLATED AND CHELATED POLY-L-LYSINE AS EFFECTOR FOR PRETARGETING IN CANCER THERAPY AND IMAGING

Objective: The aim of this study was to synthesise and evaluate polylysine-based effectors for pretargeted radioimmunotherapy and imaging. These molecules can readily be size-modified and charge-modified to decrease the renal uptake of radioactivity, which is often a major problem for small radiolabeled molecules. Several chelators and biotin molecules (for antibody-streptavidin-binding in vivo) are also easily incorporated into one structure because of the polylysine.Methods: The effectors were synthesised using poly-L-lysine, NHS-LC-biotin, CHX-A''-DTPA or p-SCN-Bn-DOTA and succinic anhydride. They were characterised, labelled with 213Bi for targeted α therapy, 68Ga for PET and 111In for SPECT, and evaluated in vitro. A kidney uptake study was performed as well with two different-sized 213Bi-labeled effectors, to evaluate how the difference in size affects the renal filtration.Results: Radiochemical purities between 97.4±0.6 % and 99.6±0.1 % and decay-corrected yields of 80.2±2.4 % after purification were achieved with the radiolabeled molecules, as well as a specific activity of 7.6 × 103GBq/µmol. The avidin binding capacity was 94.4±1.9%. The kidney uptake study demonstrated a reduction of renal absorbed dose by 80% when modifying the molecular size and charge.Conclusion: The synthesised polylysine-based effectors show potential for further in vivo evaluation in pretargeted radioimmunotherapy and imaging

Enhancing spectral quality in complex environmental matrices: Supporting suspect and non-target screening in zebra mussels with ion mobility

Identification of bioaccumulating contaminants of emerging concern (CECs) via suspect and non-target screening remains a challenging task. In this study, ion mobility separation with high-resolution mass spectrometry (IM-HRMS) was used to investigate the effects of drift time (DT) alignment on spectrum quality and peak annotation for screening of CECs in complex sample matrices using data independent acquisition (DIA). Data treatment approaches (Binary Sample Comparison) and prioritisation strategies (Halogen Match, co-occurrence of features in biota and the water phase) were explored in a case study on zebra mussel (Dreissena polymorpha) in Lake Ma center dot laren, Sweden's largest drinking water reservoir. DT alignment evidently improved the fragment spectrum quality by increasing the similarity score to reference spectra from on average (+/- standard deviation) 0.33 +/- 0.31 to 0.64 +/- 0.30 points, thus positively influencing structure elucidation efforts. Thirty-two features were tenta-tively identified at confidence level 3 or higher using MetFrag coupled with the new PubChemLite database, which included predicted collision cross-section values from CCSbase. The implementation of predicted mobility data was found to support compound annotation. This study illustrates a quantitative assessment of the benefits of IM-HRMS on spectral quality, which will enhance the performance of future screening studies of CECs in complex environmental matrices

The Human and Mouse Islet Peptidome: Effects of Obesity and Type 2 Diabetes, and Assessment of Intraislet Production of Glucagon-like Peptide-1.

To characterize the impact of metabolic disease on the peptidome of human and mouse pancreatic islets, LC-MS was used to analyze extracts of human and mouse islets, purified mouse alpha, beta, and delta cells, supernatants from mouse islet incubations, and plasma from patients with type 2 diabetes. Islets were obtained from healthy and type 2 diabetic human donors, and mice on chow or high fat diet. All major islet hormones were detected in lysed islets as well as numerous peptides from vesicular proteins including granins and processing enzymes. Glucose-dependent insulinotropic peptide (GIP) was not detectable. High fat diet modestly increased islet content of proinsulin-derived peptides in mice. Human diabetic islets contained increased content of proglucagon-derived peptides at the expense of insulin, but no evident prohormone processing defects. Diabetic plasma, however, contained increased ratios of proinsulin and des-31,32-proinsulin to insulin. Active GLP-1 was detectable in human and mouse islets but 100-1000-fold less abundant than glucagon. LC-MS offers advantages over antibody-based approaches for identifying exact peptide sequences, and revealed a shift toward islet insulin production in high fat fed mice, and toward proglucagon production in type 2 diabetes, with no evidence of systematic defective prohormone processing

Expanding distribution of lethal amphibian fungus Batrachochytrium salamandrivorans in Europe

Emerging fungal diseases can drive amphibian species to local extinction. During 2010-2016, we examined 1,921 urodeles in 3 European countries. Presence of the chytrid fungus Batrachochytrium salamandrivorans at new locations and in urodeles of different species expands the known geographic and host range of the fungus and underpins its imminent threat to biodiversity

Ambient observations of dimers from terpene oxidation in the gas phase : Implications for new particle formation and growth

We present ambient observations of dimeric monoterpene oxidation products (C16-20HyO6-9) in gas and particle phases in the boreal forest in Finland in spring 2013 and 2014, detected with a chemical ionization mass spectrometer with a filter inlet for gases and aerosols employing acetate and iodide as reagent ions. These are among the first online dual-phase observations of such dimers in the atmosphere. Estimated saturation concentrations of 10(-15) to 10(-6)mu gm(-3) (based on observed thermal desorptions and group-contribution methods) and measured gas-phase concentrations of 10(-3) to 10(-2)mu gm(-3) (similar to 10(6)-10(7)moleculescm(-3)) corroborate a gas-phase formation mechanism. Regular new particle formation (NPF) events allowed insights into the potential role dimers may play for atmospheric NPF and growth. The observationally constrained Model for Acid-Base chemistry in NAnoparticle Growth indicates a contribution of similar to 5% to early stage particle growth from the similar to 60 gaseous dimer compounds. Plain Language Summary Atmospheric aerosol particles influence climate and air quality. We present new insights into how emissions of volatile organic compounds from trees are transformed in the atmosphere to contribute to the formation and growth of aerosol particles. We detected for the first time over a forest, a group of organic molecules, known to grow particles, in the gas phase at levels far higher than expected. Previous measurements had only measured them in the particles. This finding provides guidance on how models of aerosol formation and growth should describe their appearance and fate in the atmosphere.Peer reviewe