An in-vivo pilot study into the effects of FDG-mNP in cancer in mice

Purpose Previously, fluorodeoxy glucose conjugated magnetite nanoparticles (FDG-mNPs) injected into cancer cells in conjunction with the application of magnetic hyperthermia have shown promise in new FDG-mNPs applications. The aim of this study was to determine potential toxic or unwanted effects involving both tumour cells and normal tissue in other organs when FDG-mNPs are administered intravenously or intratumourally in mice. Materials and methods FDG-mNPs were synthesized. A group of six prostate-tumour bearing mice were injected with 23.42 mg/ml FDG-mNPs (intravenous injection, n = 3; intratumoural injection into the prostate tumour, n = 3). Mice were euthanized and histological sampling of tissue was conducted for the prostate tumour, as well as for lungs, lymph nodes, liver, kidneys, spleen, and brain, at 1 hour (n = 2) and 7 days (n = 4) post-injection. A second group of two normal (non-cancerous) mice received the same injection intravenously into the tail vein and were euthanised at 3 and 6 months post-injection, respectively, to investigate if FDG-mNPs remained in organs at those time points. Results In prostate-tumour bearing mice, FDG-mNPs concentrated in the prostate tumour, while relatively small amounts were found in the organs of other tissues, particularly the spleen and the liver; FDG-mNP concentrations decreased over time in all tissues. In normal mice, no detrimental effects were found in either mouse at 3 or 6 months. Conclusion Intravenous or intratumoural FDG-mNPs can be safely administered for effective cancer cell destruction. Further research on the clinical utility of FDG-mNPs will be conducted by applying hyperthermia in conjunction with FDG-mNPs in mice

IN VIVO BONE AND SOFT TISSUE RECONSTRUCTION OF IN-SITU INJECTABLE METHACRYLATED GELATIN (GEL-MA)-NANOHYDROXYAPATITE (nHAp) AND MESENCHYMAL STEM CELL (MSCs) SYSTEMS

AbstractIn order to treat the missing teeth in the jaws with dental implants, adequate amount and quality of bone tissue is needed in the maxillo-mandibular area. In order for the treatment to be successful and to function in the mouth for a long time, alveolar bone must be adequate size. Extraction of the teeth initiates the resorption process in the alveolar bone and causes a decrease in the height and/or width of the existing bone. This study was carried out todetermine the effect of methacrylate gelatin (Gel-MA), nanoHydroxyapatite (nHAp) and mesenchymal stem cell (MSCs) systems placed in tooth extraction sockets of rats on 3D bone healing and regeneration. These systems, which are used for bone regeneration, are easy to apply and biocompatible. It has also osteoconductive and osteoinductive properties. Thirty-six Sprague-Dawley rats were randomly assigned to control/blank defect (group I, n=12), Gel- MA+nHAp (group II, n=12) and Gel-MA+ nHAp+MSCs (group III, n=12) in the study. n=12) were divided into 3 experimental groups. Immediately after the extraction of the left maxillary 1st and 2nd molars of the rats, Gel-MA+ nHAp and Gel-MA+nHAp+MSCs were placed in the extraction sockets of the rats in Group II and Group III, respectively. Hydrogel form was created by applying UV light (395-480nm, 30 sec) through the mouth on the placed biomaterials. Half of the animals in all groups were sacrificed at the 4th week and the other half at the 8th week, and tissue samples were taken from the maxilla. Macroscopic and microtomographic (micro-CT) evaluation was performed. According to the results of micro- CT analysis taken from the 4th and 8th week samples of Group III, bone volume percent (BVP) was statistically significant and had the highest value compared to the other groups. In the 4th week micro-CT analysis of Group III, the mean BVP was 81.02±3.05%, and the 8th week mean BVP value was 85.86±1.74% (p &amp;lt;0.001). According to the results of micro-CT analysis taken from tissue samples at the 4th week, a significant difference was found between group I and group III and between group II and group III (p&amp;lt;0.001, p&amp;lt;0.01). Statistically similar differences were found between the same groups at week 8 (p&amp;lt;0.001, p&amp;lt;0.05). In conclusion, it has been shown that Gel-MA+nHAp+MSCs systems are highly effective in 3D bone reconstruction in jaw bones. As a result, it was determined that Gel- MA+nHAp+MSCs systems are very effective in 3D bone reconstruction in jaw bones.Keywords: Alveolar bone, resorption, mesenchymal stem cell, methacrylated gelatin, nanoHydroxyapatite, reconstruction.</p

A promising radiolabeled drug delivery system for methotrexate: synthesis and in vitro evaluation of Tc-99m labeled drug loaded uniform mesoporous silica nanoparticles

In present study, we describe a promising radiolabeled drug delivery system for Methotrexate (MTX), an anticancer drug used in the treatment of breast cancer. Uniform and re-dispersible MSN were synthesized with a particle size of as 42.55 +/- 1.45 nm. Then, MTX was loaded into the surface modified MSN with DTPA over 95% loading capacity. Subsequently, MTX loaded MSN carrier system was radiolabeled with Tc-99 m (Tc-99m-MTX-MSN) with 92.20 +/- 0.8% radiolabeling yield. Furthermore, in vitro evaluation on estrogen positive (ER +) MCF7 and estrogen negative (ER-) A549 cells lines were performed for determining apoptotic and cytotoxic effects of MTX-MSN, and incorporation behavior of Tc-99m-MTX-MSN. Drug loaded MSN particles were exhibit higher uptake in MCF7 cells than A549 cells.Ege University Scientific Research Projects Coordination Unit [2017NBE006]The authors gratefully acknowledge the support by Ege University Scientific Research Projects Coordination Unit (Funding Number: 2017NBE006)

Examination of the Association Between 3,4-Divanillyltetrahydrofuran Lignan (Urtica dioica Origin) and Prostate Cancer Cells by I-131 Radiolabeling

WOS: 000586257000001PubMed: 32453606Background: Prostate cancer is the most common type of cancer for men in many countries. One of the various prostate cancer therapy methods is hormone therapy, and explaining the association between androgen hormones and prostate cancer is a critical role for successful prostate cancer treatment. Materials and Methods: in the current study, the behavior of 3,4- divanillyltetrahydrofuran (DTH) was examined against prostate cancer cells, which have androgen sensitivity differences [LNCaP (+), PC3 (-)]. For this aim, DTH was obtained by extraction of Urtica dioica roots. the molecular structure of isolated compound was confirmed as DTH by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy analyses. To evaluate the association of androgen sensitivity, DTH was radiolabeled with I-131, and cell uptake assay was performed by using I-131-radiolabeled DTH. Also, cytotoxicity (WST-1) assay of DTH was performed against LNCaP and PC3 cells to determinate the toxic effects of DTH on different androgen mechanisms. Results: the results of assays on cells have shown that DTH lignan behaves different like being more toxic to LNCaP cells than PC3 cells, depending on androgen sensitivity. Conclusion: the results may contribute both the research topics of phytolignan prostate cancer and androgen-sensitive prostate cancer

Preparation and characterization of radiolabeled magnetic nanoparticles as an imaging agent

Magnetic nanoparticles were prepared by a reduction-precipitation method and coated with an amino silane coupling agent. Guanine (Gua) was conjugated to the magnetic nanoparticles (MNPs) using glutaraldehyde as a cross-linker. Common techniques (Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and vibrating electron microscopy) were used to assess the properties of the particles. Structural investigations showed that amino silane-coated MNPs had a particle size of about 40-60 nm in diameter with a spherical morphology. The guanine-conjugated MNPs were radiolabeled with Tc-99m(I)-tricarbonyl core (Tc-99m(CO)(3)-MNP-Gua) with a labeling yield of 72 +/- 4 %. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of Tc-99m(CO)(3)-MNP-Gua was 98 +/- 2 % in the final solution. The biologic distribution of guanine MNPs was assessed in New Zealand rabbits using a gamma camera. In the in vivo experiment, a high level of radioactivity was observed in the lungs and liver soon after intravenous administration of Tc-99m(CO)(3)-MNP-Gua