Consider the workhorse: Nonhomologous end-joining in budding yeast

DNA double strand breaks (DSBs) are dangerous sources of genome instability and must be repaired by the cell. Nonhomologous end joining (NHEJ) is an evolutionarily conserved pathway to repair DSBs by direct ligation of the ends, with no requirement for a homologous template. While NHEJ is the primary DSB repair pathway in mammalian cells, conservation of the core NHEJ factors throughout eukaryotes make the pathway attractive for study in model organisms. The budding yeast, Saccharomyces cerevisiae, has been used extensively to develop a functional picture of NHEJ. In this review, we will discuss the current understanding of NHEJ in S. cerevisiae. Topics include canonical end-joining, alternative end-joining, and pathway regulation. Particular attention will be paid to the NHEJ mechanism involving core factors, including Yku70/80, Dnl4, Lif1, and Nej1, as well as the various factors implicated in the processing of the broken ends. The relevance of chromatin dynamics to NHEJ will also be discussed. This review illustrates the use of S. cerevisiae as a powerful system to understand the principles of NHEJ, as well as in pioneering the direction of the field.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Good beginnings: getting it right in the early years

This publication is a compendium of three papers commissioned as part of the Stewardship Dialogues for Aboriginal and Torres Strait Islander Health, a project of the Lowitja Institute. The first two were written to inform the discussion during the Stewardship Dialogues and the third was written after the Dialogues to address an identified need for decision makers to have access to a more systematic review of the evidence about the effectiveness of different interventions. These papers review the evidence on the importance of a healthy start to life and on interventions to promote good beginnings. The Stewardship Dialogues for Aboriginal and Torres Strait Islander Health were established to test if an open exploration of underlying barriers to better progress in Aboriginal and Torres Strait Islander health policy and programs can  generate new ways to approach some of the ‘wicked problems’ of policy and implementation. Dialogue participants identified that education and early years interventions, implemented in collaboration with Aboriginal and Torres Strait Islander communities and properly adapted to their settings, held the potential to produce significant long-term effects on health and wellbeing

Cost accounting: managerial use of accounting data/ Henke

xv, 943 hal.: ill.; 23 cm