Uloga kompleksa germanija s L-cysteinom i α-tokoferolom kao stimulatora antioksidativnog obrambenog sustava štakora izloženih gama-zračenju

This study was conducted to evaluate the potency of the newly prepared germanium L-cysteine α-tocopherol complex [germanium dichloro tetrakis (L-cysteinyl-α-tocopherol amide) dichloride] as a protective agent against γ-irradiation-induced free radicals production and liver toxicity. Male Swiss albino rats were injected intraperitoneally with the germanium complex in a concentration of 75 mg kg-1 body mass per dose, for 6 successive doses, last dose administered twenty minutes pre-exposure to a single dose of whole body γ-irradiation of 6.5 Gy. Lipid peroxidation (LPx), nitric oxide (NO), glutathione (GSH) levels, and activity of the antioxidant enzymes glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) were estimated in blood and liver, and blood total protein, cholesterol, triglyceride and α-tocopherol content were estimated as well. The results revealed that administration of germanium complex pre-irradiation resulted in significant (p < 0.001) improvement compared to the irradiated group in the level of hepatic and blood LPx. Hepatic GSH revealed a significant increase (p < 0.001), while its level showed no significant variation in blood. Also, the level of NO in blood and liver increased significantly (p < 0.001). On the other hand, pretreatment with the germanium complex normalized the activities of SOD, GPx and CAT in blood and liver when compared to the irradiated group. The study also documents a marked decrease in the blood triglyceride and cholesterol (p < 0.001); and significant increase (p < 0.001) of -tocopherol and total protein contents in blood. These biochemical changes were associated with marked improvement of histological status. Therefore, the germanium L-cysteine α-tocopherol complex may be a good candidate for ameliorating the changes induced by irradiation, which indicates the beneficial radio-protective role of this antioxidant agent.U radu je procjenjivan kompleks germanija s L-cisteinom i α-tokoferolom [germanijev diklortetrakis (L-cisteinil-α-tokoferol amid) diklorid] kao zaštitno sredstvo protiv slobodnih radikala induciranih γ-zračenjem hepatotoksičnosti. Mužjacima švicarskih albino štakora davan je intraperitonealno kompleks germanija, u 6 sukcesivnih doza po 75 mg kg-1 tjelesne mase, posljednja doza dana je dvadeset minuta prije izlaganja cijelog organizma jednokratnoj dozi γ-ozračivanja od 6,5 Gy. U krvi i jetri praćena je razina lipidne peroksidacije (LPx), dušikovog(II) oksida (NO), glutationa (GSH), aktivnost antioksidativnih enzima glutation peroksidaze (GPx), superoksid dismutaze (SOD) i katalaze (CAT), te količina ukupnih proteina u krvi, kolesterola, triglicerida i α-tokoferola. Rezultati su pokazali da je primjena germanijevog kompleksa značajno (p < 0,001) poboljšala koncentraciju jetrenih i krvnih LPx. Koncentracija GSH u jetri je značajno porasla (p < 0,001), dok se njegova razina u krvi nije značajno promijenila. Koncentracija NO u krvi i jetri značajno se smanjila (p < 0,001). S druge strane, prethodna obrada s kompleksom germanija normalizirala je aktivnost SOD, GPx i CAT u krvi i jetri u odnosu na ozračenu skupinu. Istraživanja su također pokazala značajno smanjenje triglicerida i kolesterola (p < 0,001) i značajno povećanje (p < 0,001) alfa-tokoferola i ukupnih proteina u krvi. Te biokemijske promjene su povezane s izraženim poboljšanjem histoloških promjena u odnosu na ozračenu skupinu. Opisani kompleks germanija mogao bi se kao antioksidativno sredstvo potencijalno upotrijebiti za spriječavanje promjena uzrokovanih zračenjem

Oncogenic challenge of bromocriptine and L-arginine versus conventional antidiabetics on diethyl nitrosamine-induced liver tumorigenesis in diabetic rats: focus on AMPK activation

Background: Diabetes mellitus (DM) is associated with a spectrum of cancers where the metabolic antecedents, consequences, and therapy might affect cancer risk. The association between hepatocellular carcinoma (HCC) and DM had been confirmed. Approaches to HCC prevention focus on the molecular regulators of the disease process defined as the inflammation-fibrosis-cancer axis. The AMP-activated protein kinase (AMPK) is an interesting metabolic tumor suppressor and a promising target for cancer prevention and therapy. This study aimed to investigate the effects of bromocriptine mesylate and L-arginine on hepatic carcinogenesis on a rat model of hepatic neoplasia induced by diethyl nitrosamine (DENA) and promoted by type-2 DM in contrast to the conventional antidiabetics.Methods: One hundred male Wistar rats were randomly assigned into two sets; control set (normal, HCC, DM, and combined HCC/DM) and treated set where rats received one of the following drugs for another 5 weeks: insulin glargine, glimepiride, metformin, pioglitazone, bromocriptine mesylate, or L-arginine. Bodyweight changes, blood glucose level, liver functions tests, serum C-peptide and alpha-fetoprotein (AFP), and hepatic activated AMPK were assessed beside the hepatic histopathological changes.Results: Equivalent to metformin, bromocriptine and L-arginine treatment significantly reduced AFP, despite their minor glycemic control. L-arginine induced AMPK activation, yet less than metformin. Histopathologic examination revealed a reduction in hepatic intra-lobular chronic inflammatory cell infiltration, steatosis and necrosis by metformin, bromocriptine, and L-arginine. Hepatic necro-inflammatory changes were most prominent in insulin-treated rats.Conclusions: L-arginine and bromocriptine mesylate prevent early neoplastic changes almost equivalent to metformin at least partially via hepatic AMPK activation

Evaluation of some phenolic extracts against aphids (Aphis craccivora) Koch under laboratory conditions

Local farmers worldwide have complained in recent years that insect pests have become resistant to the majority of insecticides, owing to pesticide abuse. In addition, highly poisonous and harmful substances may cause health and environmental dangers. Friendly alternatives such as plant extracts are the main targets as substituents to synthetic pesticides. The present study aimed to extract total phenols from some plants and evaluate their efficacy on aphids, Aphis craccivora, under laboratory conditions. Four methanolic plant extracts from Punica granatum, Lantana camara, Portulaca oleracea and Ziziphus jujuba, containing phenolic components were evaluated against A. craccivora through: slide dipping, spraying, and leaf dipping techniques. Generally, positive relationships between the concentrations of the tested phenolic extracts and their mortality percentages were noticed in the case of slide dipping and spraying techniques. Conversely, no biological efficacy was found using the leaf dipping technique. The descending order of effectiveness of the tested extracts depending on their EC50 values was 0.017, 0.321, 1.142 and 16.114 ppm for Z. jujuba, P. oleraceae P. granatum and L. comara, respectively, in the case of the slide dipping technique. In contrast, P. granatum, L. camara, P. oleraceae and Z. jujuba had EC50 values of 0.0023, 0.017, 0.321 and 2.3409 ppm, respectively, in the case of the spraying technique. Additionally, a direct proportion was found between mortality percentages and treatment period for plant extracts under study with both techniques. After formulation and completion of additional essential field research, phenols isolated from the plants under study could be employed to combat A. craccivora

Kemijski sastav sojeva plijesni Monascus ruber

Chemical profile of Monascus ruber strains has been studied using gas chromatography-mass spectrometry (GC/MS) analysis. The colour intensity of the red pigment and secondary metabolic products of two M. ruber strains (AUMC 4066 and AUMC 5705) cultivated on ten different media were also studied. Metabolic products can be classified into four categories: anticholesterol, anticancer, food colouring, and essential fatty acids necessary for human health. Using GC/MS, the following 88 metabolic products were detected: butyric acid and its derivatives (25 products), other fatty acids and their derivatives (19 products), pyran and its derivatives (22 products) and other metabolites (22 products). Among these, 32 metabolites were specific for AUMC 4066 strain and 34 for AUMC 5705 strain, whereas 22 metabolites were produced by both strains on different tested substrates. Production of some metabolites depended on the substrate used. High number of metabolites was recorded in the red pigment extract obtained by both strains grown on malt broth and malt agar. Also, 42 aroma compounds were recorded (4 alcohols, 2 benzaldehydes, 27 esters, 3 lactones, 1 phenol, 1 terpenoid, 3 thiol compounds and acetate-3-mercapto butyric acid). Thin layer chromatography and GC/MS analyses revealed no mycotoxin citrinin in any media used for the growth of the two M. ruber strains.Svrha je rada bila proučiti kemijski sastav sojeva plijesni Monascus ruber pomoću plinske kromatografije i masene spektrometrije. Ispitan je i intenzitet crvenog pigmenta te sekundarni produkti metabolizma dvaju sojeva plijesni M. ruber (AUMC 4066 i AUMC 5705) uzgojenih na deset različitih podloga. Produkti metabolizma mogu se razvrstati u 4 skupine: spojevi koji snižavaju razinu kolesterola, antikancerogeni spojevi, prehrambene boje i esencijalne masne kiseline neophodne za ljudsko zdravlje. Plinskom je kromatografijom i masenom spektrometrijom pronađeno 88 produkata metabolizma, a među njima maslačna kiselina i njezini derivati (25), ostale masne kiseline i njihovi derivati (19), piran i njegovi derivati (22), te ostali metaboliti (22). Od ukupnog broja produkata, njih 33 je specifično za soj AUMC 4066, a 34 za soj AUMC 5705, dok su oba soja proizvela 22 produkta na različitim podlogama. Proizvodnja je produkata ovisila o upotrijebljenoj podlozi. Velik je broj metabolita pronađen u ekstraktima crvenog pigmenta iz oba soja uzgojena na podlogama od slada. Pronađena su 42 aromatska spoja, i to 4 alkohola, 27 estera, 3 laktona, 1 fenol, 1 terpenoid, 3 tiola i 1 merkapto spoj. Tankoslojnom i plinskom kromatografijom te masenom spektrometrijom nije pronađen mikotoksin citrinin ni u jednoj podlozi na kojoj su rasla ova dva soja plijesni M. ruber

The serum thyroid hormone profile in mechanically ventilated children: Does euthyroid sick syndrome exist?

Background: The term "euthyroid sick syndrome" refers to alterations in thyroid function testing during critical illness. Mechanically ventilated children's thyroid hormone levels may be altered, although the reason for this remains a mystery.Objective: This study was aimed to evaluate thyroid hormone profile in mechanically ventilated children and their correlation with mortality.Patients and Methods: Thirty-four mechanically ventilated children were enrolled in a prospective cohort study. On the first and third days of mechanical ventilation, serum TSH, FT3, FT4 and reverse T3 were measured.Results: The mean age of the studied patients was of 31.06 ± 35.94 months. After three days of mechanical ventilation, the serum levels of FT3 and FT4 in the blood were significantly lower than the serum levels on the first day. The serum reverse T3 levels increased significantly on the third day of mechanical ventilation compared to the first day. There was a statistically significant increase in the number of patients with low FT3 and FT4 serum levels on the third day compared to the first day. Twenty-two patients (64.7%) died, and the frequency of low FT3 among dead patients was signiisignificantlycantly higher than among surviving patients.Conclusions: It could be concluded that the mechanically ventilated children had signs of euthyroid sick syndrome, shown by low levels of FT3 and FT4 and a rise in rT3 without a compensatory rise in TSH. Serum FT3 and FT4 decreased, but reverse T3 increased, on the third day of mechanical ventilation compared to the first day. Patients who had low FT3 levels died at a higher rate

Bone Mineral Density Changes in Patients with Recent-Onset Rheumatoid Arthritis

Background Osteoporosis and related fragility fractures are one of the most common complications seen in patients with rheumatoid arthritis (RA) and dramatically affect quality of life. Objective To evaluate changes in bone mineral density in patients with recent onset rheumatoid arthritis (< 1 year) and its correlation if any with a modified DAS-28 score and simple erosion narrowing score (SENS). Methods This study included 30 patients with recent-onset rheumatoid arthritis fulfilling the new American College of Rheumatology/European League Against Rheumatism diagnostic criteria for rheumatoid arthritis and 20 healthy volunteers as controls. All were subjected to a complete blood count, erythrocyte sedimentation rate, C-reactive protein, liver function tests, renal function tests, rheumatoid factor, and plain x-rays of the hands and feet. Dual-energy x-ray absorptiometry DEXA was used to measure bone mineral density (BMD) of the left proximal femur, lumbar spine (L1–L4), and lower distal radius at the time of recruitment. Results In the RA patients, 13.3% had osteoporosis, 50% had osteopenia, and 36.7% had normal BMD. The most common site of osteoporosis was the lumbar spine (four patients, 13.3%) followed by the femur (two patients, 6.6%), and forearm (only one patient, 3.3%). There was a significantly higher percentage of osteoporosis among RA males than females and the difference was statistically significant ( P = 0.009). Osteoporosis was more common in patients treated with corticosteroids and disease modifying antirheumatic drugs (DMARDs) than in patients treated with only nonsteroidal anti-inflammatory drugs ( P = 0.004). Higher disease activity (DAS-28) was found in RA patients with osteoporosis compared to RA patients with normal BMD or osteopenia, but the difference was not statistically significant. Osteoporotic RA patients were found to have a higher SENS score for radiological damage than nonosteoporotic ones. Conclusion BMD changes do occur in patients with early RA, and are not necessarily correlated with disease activity (DAS-28). However, a significant negative correlation was found between BMD and the score of radiological damage (SENS). Dual energy x-ray absorptiometry is an important investigation to assess BMD in early RA patients

Pharmaceutical Equivalence of Some Conventional Carbamazepine Tablets Marketed in Sudan

Background: Carbamazepine (CBZ) is commonly used in the treatment and control of epilepsy, seizures, and neuropathic pain. Due to its limited water solubility, CBZ have slow and variable&nbsp;absorption following oral administration. Effective CBZ plasma levels&nbsp;are achieved through multiple-dose administration of conventional CBZ tablets&nbsp; which may result in serious side effects because of its narrow therapeutic index and toxicity levels.&nbsp;Objectives: This work aimed at&nbsp;comparing four commercial brands of CBZ tablets (A, B, C and D) manufactured by multinational and national companies including the originator (A) through evaluation of their pharmaceutical equivalence using pharmacoepial and nonpharmacoepial standard tests. Methods: Model-independent approach was used for determination of dissolution efficiency (% D.E) and fit factors. &nbsp;Difference&nbsp;between brands was demonstrated through analysis of difference (f1) and similarity (f2) data. In addition various quality tests including weight variation, thickness, diameter, hardness, friability and disintegration time were carried out. Results: The study revealed that all brands complied with the USP specifications regarding weight variation, friability disintegration and drug content. The amount of drug released within 45 minutes were found satisfactory and ranged from 83.44% to 94.5%.&nbsp;Although clear differences in release profiles exist, all&nbsp;brands released about 90% of the labeled CBZ within 30 minutes which can satisfy the patient need.&nbsp;Only brand B failed to pass the nonpharmacoepial hardness test. Conclusion: Selected brands of CBZ tablets complied with all required pharmacoepial quality specifications

Performance evaluation of PI controlled series stacked power delivery architectures for high-efficiency data centers

Series-stacked architectures have been successfully deployed for data center applications at substantially higher efficiencies than conventional power delivery architectures. In the series-stacked architectures, servers are series-connected electrically to reduce the high step-down conversion stage of voltage utilized in the conventional architectures. Differential power processing converters are, therefore, used to regulate the servers’ voltages and compensate for the unpredicted mismatch between servers’ currents. The main contribution of this paper comprises novel control approaches based on PI controllers purposeful for the two architectures that have reported the highest reliability and efficiency in differential power processing namely: server-to-bus and server-to-virtual bus. Both systems employ a dual active bridge (DAB) converter to accommodate the fluctuating loads of each server. Unlike hysteresis current/voltage control commonly employed in the available literature, the proposed control approaches offer less complexity, lower harmonics, and higher immunity towards the noise, thus no need for high-quality sensors to successfully achieve voltage balance and/or optimal string current flow. Moreover, a comparative study has been structured between the investigated series-stacked architectures under the proposed PI control approaches showing the merits and the demerits of each architecture. The proposed controllers have been validated based on simulations and experimentally

Osteogenic Differentiation Potential of Human Bone Marrow and Amniotic Fluid-Derived Mesenchymal Stem Cells in Vitro & in Vivo

BACKGROUND: Cell therapies offer a promising potential in promoting bone regeneration. Stem cell therapy presents attractive care modality in treating degenerative conditions or tissue injuries. The rationale behind this is both the expansion potential of stem cells into a large cell population size and its differentiation abilities into a wide variety of tissue types, when given the proper stimuli. A progenitor stem cell is a promising source of cell therapy in regenerative medicine and bone tissue engineering. AIM: This study aimed to compare the osteogenic differentiation and regenerative potentials of human mesenchymal stem cells derived from human bone marrow (hBM-MSCs) or amniotic fluid (hAF-MSCs), both in vitro and in vivo studies. SUBJECTS AND METHODS: Human MSCs, used in this study, were successfully isolated from two human sources; the bone marrow (BM) and amniotic fluid (AF) collected at the gestational ages of second or third trimesters. RESULTS: The stem cells derived from amniotic fluid seemed to be the most promising type of progenitor cells for clinical applications. In a pre-clinical experiment, attempting to explore the therapeutic application of MSCs in bone regeneration, Rat lumbar spines defects were surgically created and treated with undifferentiated and osteogenically differentiated MSCs, derived from BM and second trimester AF. Cells were loaded on gel-foam scaffolds, inserted and fixed in the area of the surgical defect. X-Ray radiography follows up, and histopathological analysis was done three-four months post- operation. The transplantation of AF-MSCs or BM-MSCs into induced bony defects showed promising results. The AF-MSCs are offering a better healing effect increasing the likelihood of achieving successful spinal fusion. Some bone changes were observed in rats transplanted with osteoblasts differentiated cells but not in rats transplanted with undifferentiated MSCs. Longer observational periods are required to evaluate a true bone formation. The findings of this study suggested that the different sources; hBM-MSCs or hAF-MSCs exhibited remarkably different signature regarding the cell morphology, proliferation capacity and osteogenic differentiation potential CONCLUSIONS: AF-MSCs have a better performance in vivo bone healing than that of BM-MSCs. Hence, AF derived MSCs is highly recommended as an alternative source to BM-MSCs in bone regeneration and spine fusion surgeries. Moreover, the usage of gel-foam as a scaffold proved as an efficient cell carrier that showed bio-compatibility with cells, bio-degradability and osteoinductivity in vivo

The Palestinian Terrestrial Vertebrate Fauna Preserved at the Biology Exhibitions of the Universities of the Gaza Strip

The Gaza Strip (365 km2 ) of Palestine (27,000 km2 ) is home to a wealth of terrestrial vertebrate fauna. Some of these faunistic species find their ways to preservation at the local universities. Hence, the current study comes to document the Palestinian terrestrial vertebrate fauna acquired by the biology exhibitions (BEs) of Al-Azhar University, Islamic University of Gaza and Al-Aqsa University that are located at the Gaza City of the Gaza Strip. The amphibians, reptiles, birds and mammals preserved at BEs of the universities in question were surveyed and scientifically classified during a three-month period extending from January to March, 2012. The study showed that all BEs of local universities are underdeveloped, lacking attention and suffer from specimen scarcity and good preservation. The BE at Al-Azhar University is the best in the arrangement and preservation of bird specimens. A total number of 200 specimens belonging to 54 terrestrial vertebrate fauna species, 39 families and 17 orders was recorded at BEs. Reptiles constituted 40.7% of the total species recorded, followed by birds (38.9%), mammals (14.8%) and amphibians (5.6%). The Islamic University of Gaza was considered the best in terms of the number of preserved species (39.8%), followed by Al-Azhar University (36.3%) and Al-Aqsa University (23.9%). The Common Toad (Bufo viridis) was the most preserved among the amphibian species recorded. Squamata was the biggest reptilian order, comprising 20 species (8 lizards and 12 snakes), with the Syrian Black Snake (Coluber jugularis asianus) was the commonest. The Palestine Viper (Vipera palaestinae) is endemic to Palestine and most venomous and dangerous to human health. The Great White Pelican (Pelecanus onocrotalus) was the largest Palestinian bird preserved at BE of Al-Azhar University. The Egyptian Mongoose (Herpestes ichneumon) and the Common Badger (Meles meles) were the biggest mammalian specimens preserved, while the Palestine Mole-rat (Spalax leucodon ehrenbergi) was the only Palestine endemic species encountered among the preserved mammals. Finally, the improvement of BEs of local universities and the construction of a Central Museum of Natural History is highly recommended in order to change the Palestinians’ attitudes toward a sustainable ecological conservation in the Gaza Strip