256 research outputs found
Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida
Genetic modification of the flavonoid pathway has been used to produce novel colours and colour patterns in ornamental plants as well as to modify the nutritional and pharmaceutical properties of food crops. It has been suggested that co-ordinate control of multiple steps of the pathway with the help of regulatory genes would lead to a more predictable control of metabolic flux. Regulation of anthocyanin biosynthesis has been studied in a common ornamental plant, Gerbera hybrida (Asteraceae). An R2R3-type MYB factor, GMYB10, shares high sequence similarity and is phylogenetically grouped together with previously characterized regulators of anthocyanin pigmentation. Ectopic expression of GMYB10 leads to strongly enhanced accumulation of anthocyanin pigments as well as to an altered pigmentation pattern in transgenic gerbera plants. Anthocyanin analysis indicates that GMYB10 specifically induces cyanidin biosynthesis in undifferentiated callus and in vegetative tissues. Furthermore, in floral tissues enhanced pelargonidin production is detected. Microarray analysis using the gerbera 9K cDNA array revealed a highly predicted set of putative target genes for GMYB10 including new gene family members of both early and late biosynthetic genes of the flavonoid pathway. However, completely new candidate targets, such as a serine carboxypeptidase-like gene as well, as two new MYB domain factors, GMYB11 and GMYB12, whose exact function in phenylpropanoid biosynthesis is not clear yet, were also identified
Mass measurements in the vicinity of the doubly-magic waiting point 56Ni
Masses of 56,57Fe, 53Co^m, 53,56Co, 55,56,57Ni, 57,58Cu, and 59,60Zn have
been determined with the JYFLTRAP Penning trap mass spectrometer at IGISOL with
a precision of dm/m \le 3 x 10^{-8}. The QEC values for 53Co, 55Ni, 56Ni, 57Cu,
58Cu, and 59Zn have been measured directly with a typical precision of better
than 0.7 keV and Coulomb displacement energies have been determined. The Q
values for proton captures on 55Co, 56Ni, 58Cu, and 59Cu have been measured
directly. The precision of the proton-capture Q value for 56Ni(p,gamma)57Cu,
Q(p,gamma) = 689.69(51) keV, crucial for astrophysical rp-process calculations,
has been improved by a factor of 37. The excitation energy of the proton
emitting spin-gap isomer 53Co^m has been measured precisely, Ex = 3174.3(10)
keV, and a Coulomb energy difference of 133.9(10) keV for the 19/2- state has
been obtained. Except for 53Co, the mass values have been adjusted within a
network of 17 frequency ratio measurements between 13 nuclides which allowed
also a determination of the reference masses 55Co, 58Ni, and 59Cu.Comment: 14 pages, 13 figures, submitted to Phys. Rev.
Electron-capture branch of 100Tc and tests of nuclear wave functions for double-beta decays
We present a measurement of the electron-capture branch of Tc. Our
value, , implies that the
Mo neutrino absorption cross section to the ground state of Tc
is roughly one third larger than previously thought. Compared to previous
measurements, our value of prevents a smaller disagreement with
QRPA calculations relevant to double- decay matrix elements
Using the EU Erasmus lifelong learning programme (LLP) for international education in horticulture: the intensive programme āBiosushort: potentials of using biodiversity for achieving a sustainable horticultureā
Total Absorption Spectroscopy Study of Rb Decay: A Major Contributor to Reactor Antineutrino Spectrum Shape
The antineutrino spectra measured in recent experiments at reactors are
inconsistent with calculations based on the conversion of integral beta spectra
recorded at the ILL reactor. Rb makes the dominant contribution to the
reactor spectrum in the 5-8 MeV range but its decay properties are in question.
We have studied Rb decay with total absorption spectroscopy. Previously
unobserved beta feeding was seen in the 4.5-5.5 region and the GS to GS feeding
was found to be 87.5(25)%. The impact on the reactor antineutrino spectra
calculated with the summation method is shown and discussed.Comment: 6 pages, 3 figure
Benign afebrile cluster convulsions with gastroenteritis: an observational study
BACKROUND: The occurrence of afebrile seizures in association with viral gastroenteritis, without dehydration or electrolyte imbalance, is virtually unknown outside Asia. They are reported to have a benign prognosis and not to require specific investigations or therapy. METHODS: We report the occurrence of such afebrile convulsions in association with viral gastroenteritis without dehydration or electrolyte imbalance, over a 3-year period, in a cohort of 14 British children. RESULTS: The children (5 males and 9 females, 10 Caucasians and 4 Asians) were aged 9 to 60 months (median 14.5 months). All 14 had a normal neurological examination and normal serum biochemistry. Twelve children had generalised seizures and 2 had, in addition, absence seizures. The number of seizures per child ranged from 1 to 8. Most convulsions were short with 85.7% of children having the longest seizure not longer than 4 minutes. The longest duration for a seizure was 10 minutes and occurred in 2 children. Convulsions did not recur after the first day in 10 children, 3 children had recurrences the second day and one child on the fourth day. No convulsions recurred after 4 days. Cerebrospinal fluid studies, computed tomography and electroencephalogram (EEG) were performed on two children who had prolonged seizures and the results were normal. No pathogenic bacteria were grown in any of the stools. Enzyme immunoassay detection of Rotavirus in the stools was positive in 7 of the 10 children where it was tested. All 14 children recovered spontaneously within a few days. On long-term follow of up to 31 months (median 16 months), none had further convulsions and all had normal development milestones. CONCLUSIONS: Afebrile seizures in association with viral gastroenteritis do also occur outside Asia. Recognition of this entity should lead to reassurance of the parents. As in previously published series, investigations such as lumbar puncture, neuroimaging and EEG are usually normal and may not be necessary in most cases. Likewise, published data indicate that long-term anticonvulsant therapy is not usually warranted and the prognosis seems to be reassuring
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