254 research outputs found
ENHANCING DISSOLUTION RATE OF INDOMETHACIN BY IN SITU CRYSTALIZATION; DEVELOPMENT OF ORALLY DISINTEGRATING TABLETS
Objective: The main objective of this study was to investigate the potential of in situ crystallization of indomethacin, in presence or absence of hydrophilic materials, to improve drug dissolution with the goal of developing fast disintegrating tablets.Methods: Indomethacin crystals were prepared by bottom up approach. Water containing hydrophilic additive (polymer or/and surfactant) was added to ethanolic solution of indomethacin while stirring. The selected polymers were hydroxylpropylmethyl cellulose E5 (HPMC E5), polyethylene glycol 6000 (PEG6000) and polyvinylpyrrolidone K40 (PVP K40). The surfactants used were Tween80 and Glucire 44/14. The precipitated particles were collected and air dried. Solid state characterization were performed in addition to in vitro release studies in both acidic (0.1 N HCL) and alkaline medium (phosphate buffer pH 6.8). Optimized formulation was selected to develop fast disintegrating tablets.Results: Thermal behavior suggested modulation in crystalline nature with reduction in particle size that was confirmed by X-ray diffraction results. Infrared spectroscopy excluded any interaction between drug and hydrophilic excipients. Drug dissolution in acid media showed slight improve in drug release, while marked increase was observed in the alkaline media. Combination between Tween80 and HPMC (F7) showed the best dissolution parameters with 5-folds enhancement in release efficiency (RE) compared to pure drug. Formula F7 was successively used to formulate fast disintegrating tablets with prompted release of 58% of the loaded dose and RE of 83%.Conclusion: In situ crystallization of indomethacin is a good approach for enhanced dissolution rate with the presence of hydrophilic additives during precipitation process improving the efficiency
A genomewide association study in divergently selected lines in rabbits reveals novel genomic regions associated with litter size traits
[EN] Uterine capacity (UC), defined as the total number of kits from unilaterally ovariectomized does at birth, has a high genetic correlation with litter size. The aim of our research was to identify genomic regions associated with litter size traits through a genome-wide association study using rabbits from a divergent selection experiment for UC. A high-density SNP array (200K) was used to genotype 181 does from a control population, high and low UC lines. Traits included total number born (TNB), number born alive (NBA), number born dead, ovulation rate (OR), implanted embryos (IE), and embryo, foetal and prenatal survivals at second parity. We implemented the Bayes B method and the associations were tested by Bayes factors and the percentage of genomic variance (GV) explained by windows. Different genomic regions associated with TNB, NBA, IE, and OR were found. These regions explained 7.36%, 1.27%, 15.87%, and 3.95% of GV, respectively. Two consecutive windows on chromosome 17 were associated with TNB, NBA, and IE. This genomic region accounted for 6.32% of GV of TNB. In this region, we found the BMP4, PTDGR, PTGER2, STYX and CDKN3 candidate genes which presented functional annotations linked to some reproductive processes. Our findings suggest that a genomic region on chromosome 17 has an important effect on litter size traits. However, further analyses are needed to validate this region in other maternal rabbit lines.The work was funded by project AGL2014-55921-C2-1-P from the National Programme for Fostering Excellence in Scientific and Technical Research Project I+D. B. Samuel Sosa-Madrid was supported by a grant from the National Secretariat of Science, Technology, and Innovation of Panama (SENACYT) for a master's degree (the first stage of this study): BECA-2199-40-2012. Also, he was supported by an FPI grant from the Ministry of Economy and Competitiveness of Spain (the second stage of this study): BES-2015-074194.Sosa-Madrid, BS.; Santacreu Jerez, MA.; Blasco Mateu, A.; Fontanesi, L.; Pena, R.; Ibáñez-Escriche, N. (2020). A genomewide association study in divergently selected lines in rabbits reveals novel genomic regions associated with litter size traits. Journal of Animal Breeding and Genetics. 137(2):123-138. https://doi.org/10.1111/jbg.12451S1231381372Aken, B. L., Ayling, S., Barrell, D., Clarke, L., Curwen, V., Fairley, S., … Searle, S. M. J. (2016). The Ensembl gene annotation system. Database, 2016, baw093. doi:10.1093/database/baw093Al-Samerria, S., Al-Ali, I., McFarlane, J. R., & Almahbobi, G. (2015). The impact of passive immunisation against BMPRIB and BMP4 on follicle development and ovulation in mice. REPRODUCTION, 149(5), 403-411. doi:10.1530/rep-14-0451Argente, M. J., Merchán, M., Peiró, R., García, M. L., Santacreu, M. A., Folch, J. 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Power Analysis of Artificial Selection Experiments Using Efficient Whole Genome Simulation of Quantitative Traits. Genetics, 199(4), 991-1005. doi:10.1534/genetics.115.175075Konno, T., Pinho Melo, E., Lopes, C., Mehmeti, I., Lenzen, S., Ron, D., & Avezov, E. (2015). ERO1-independent production of H2O2 within the endoplasmic reticulum fuels Prdx4-mediated oxidative protein folding. Journal of Cell Biology, 211(2), 253-259. doi:10.1083/jcb.201506123Laborda, P., Mocé, M. L., Blasco, A., & Santacreu, M. A. (2012). Selection for ovulation rate in rabbits: Genetic parameters and correlated responses on survival rates1. Journal of Animal Science, 90(2), 439-446. doi:10.2527/jas.2011-4219Laborda, P., Mocé, M. L., Santacreu, M. A., & Blasco, A. (2011). Selection for ovulation rate in rabbits: Genetic parameters, direct response, and correlated response on litter size1. 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Stimulated perturbation on the neutron flux distribution in the mutually-dependent source-to-absorber geometry
The complexity of the neutron transport phenomenon throws its shadows on
every physical system wherever neutron is produced or absorbed. The Monte Carlo
N-Particle Transport Code (MCNP) was used to investigate the flux perturbations
in the neutron field caused by an absorber. The geometry of the present
experiment was designed to reach a simulation of an isotopic neutron field. The
neutron source was a AmBe with the production physics of neutrons is
dependent only on alpha-beryllium interaction and is independent of what
happened to the neutron after it was generated. The geometries have been
designed to get a volume of uniform neutron densities within a spherical volume
of radius 15 cm in every neutron energy group up to 10 MeV. Absorbers of
different dimensions were placed within the volume to investigate the field
perturbation. Different neutron absorbers were used to correlate the phenomenon
to the integral cross-section of the absorber. Flux density inside and outside
the absorber samples was determined, while the spatial neutron flux
distribution produced by the AmBe source without an absorber was taken as a
reference. This study displayed that absorbers of various dimensions perturb
the neutron field in a way that is dependent on the absorption and scattering
cross-sections, particularly in the neutron resonance region. Unlike the simple
picture of reducing the number density of neutrons, the perturbation was found
to influence the moderation of neutrons in the medium, significantly above 1
MeV.Comment: 10 pages, 13 figures, 26 reference
Social Media Usage and Women's Empowerment in Qatar: Evidence from a National Representative Survey
Introduction Social media usage has grown in importance over the past decade, particularly in Middle East. During this period, it has come to be recognized as more than a tool for social connections between individuals, with research demonstrating how it can empowering people socially, politically and economically[1]. Past research has demonstrated the impact of social media in areas as diverse as such as healthcare provision, political participation, and marketing[2]. However, how and to what extent social media usage contributes to women's empowerment in the Arab region has not been thoroughly examined. Academic and non-academic studies alike have focused heavily on a few well-known cases of social media activism and woman's rights, such as, women driving in Saudi Arabia[3] or the Arab Spring in Egypt[4]. These studies raise a number of relevant questions. Is social media potent in the population more broadly, or is it just a tool for activists and polemics? If it does empower women, does it do so by shaping deeply rooted attitudes and norms or does it mere reflect the value of the general population? How can we understand the role of traditional education in shaping attitudes and values in light of the large volumes of readily available information provided by social media? The current paper examines social media usage in Qatar and its influences on attitudes toward women's empowerment and considers the complex role of education. We use a national representative survey data from a 2017 survey of Qatari nationals to test our expectations. In the last twenty years, Qatar has experienced a significant shift in economic development, and education accessibility. The State of Qatar has widely sought to include women in public life, particularly in the process of decision-making. Yet, the debate about the role of social media in increasing women's awareness of their equal rights in holding positions of power and contributing to their political and economic empowerment is not widely discussed in Qatar. Thus, Qatar is a case where researchers might expect to find changing attitudes in spite of the absence of major social media feminist activists. The paper also explores a number of trends emerging in the analysis of social media usage, across demographic groups such as age, gender, marital status and socio-economic differences including education levels and household income. Methodology A questionnaire was initially designed in English and then translated into Arabic by the Social and Economic Survey Research Institute (SESRI) to collect all necessary information related to the study. In this survey, the target population included people who are 18 years or older and live in residential housing units in Qatar during the survey reference period (May 6th-27th, 2017). It includes groups of Qataris and expatriates, though the analysis in this paper focuses on Qataris. It is important to note that while preparing the sample for the survey, the Qatari population sub-group was over sampled in order to ensure that Qataris were well represented in the survey. The survey was administered in CAPI (computer assisted data collection) method for face to face interviews. The interviews were conducted at the home of the respondent. In terms of data analysis, all individual interviews were merged and saved in a single BLAISE data file. This dataset was then cleaned, coded and saved in STATA formats for analysis. Analysis of the data included order logistical regression with appropriate calculation of interactive effects and predicted probabilities for interpretation. Findings and Implications Data from the 2017 survey are newly available and thus the findings discussed here are preliminary. We find that social media usage in the examined population is related to increased support for woman in community leadership among Qatari females but not males. This finding is robust to a number of statistical controls, including for the respondent's level of education. In fact, a respondent's level of formal education has much less predictive power than social media usage among female Qataris. Furthermore, we find that social media usage is only weakly related to political variables, such as interest in politics. This suggests that social media may be working to empower women first through changing gender attitudes and only secondly through engagement with the political sphere. These findings are relevant to both academics who are seeking to understand the mechanisms through which social media may empower women and policymakers in Qatar who are concerned with advancing their welfare. It shows that woman can use social media to find their voice and engage with the public sphere, even where political activism has not been common. [1] Dubai School of Government, "Arab Social Media Report, Vol 1, No. 3, November 2011. [2] TNS, "Arab Social Media Report", First Report 2015. [3] Begum, Rothna, "The Brave Female Activists Who Fought to Lift Saudi Arabia's Driving Ban", News Deeply, September 29th, 2017. [4] Tufekci, Zeynep and Wilson, Christopher, "Social Media and the Decision to Participate in Political Protest: Observations From Tahrir Square", Journal of Communication, 62 (2012) 363-37.qscienc
Genomic and serum tumor markers in Egyptian females with and without family cancer history
OBJECTIVE: Multiple variables affect the probability of development of cancer. The present study aimed to screen Egyptian females for early prognostic cancer markers such as carcinoembryonic antigen (CEA), the soluble form of transmembrane mucin protein (CA15-3), MUC1 and important sex hormones (Progesteron, Oestrogen, and Prolactin) and three germline BRCA1/2 founder mutations.
PATIENTS AND METHODS: Forty-five DNA samples were screened for 185delAG and 5382insC in the BRCA1 and 6174delT in the BRCA2 genes using polymerase chain reaction (PCR)-directed mutagenesis. Each sample of the 185delAG and the 6174delT mutations was confirmed using Restriction Fragment Length Polymorphism (RFLP) analysis. Nine suspected PCR products of 185delAG and the forty-five amplicons of 6174delT mutations were further confirmed using Sanger sequencing. Sex hormones (Progesteron, Oestrogen, and Prolactin) and cancer antigens (CA 15-3 and CEA) concentrations were quantitatively determined in serum samples using ELISA.
RESULTS: We found significant associations only for oestrogen (p-value=0.036), while non-significant (p-value= 0.123) hyperprolactinemia with cancer history. But none of the individuals carried the BRCA1/2 studied mutations while new variants were detected; (delA) in position 93865, deletion (delA) or substitution of A by G (A/G) in position 93858 and (insA) in position 93844 with frequency of 50%, 50%, 25% and 25%, respectively, in subjects with cancer history.
CONCLUSIONS: The serum level of oestrogen could be a useful non-invasive cancer marker while significant association of hyperprolactinemia and the new BRCA1/2 variants with cancer needs extra study
Integrating production scheduling and transportation procurement through combinatorial auctions
This study uses the winner determination problem (WDP) to integrate auction transportation procurement with decisions related to production scheduling. The basic problem arises when a manufacturer has to clear a combinatorial auction to decide whether to cover transportation needs by using the in-house fleet or to procure transportation through auction. Thus, the manufacturer should include an additional decision level by integrating the WDP with production scheduling to gain efficiency and achieve savings in the logistics system. To the best of our knowledge, this is the first time production and transportation procurement problems are being solved simultaneously in an integrated manner. The study proposes a mathematical formulation and develops two heuristic approaches for solving the integrated problem. Extensive computational experiments and sensitivity analyses are reported to validate the model, assess the performance of the heuristics, and show the effect of integration on total cost. © 2020 The Authors. Networks published by Wiley Periodicals LLC
Criticality Analysis of Activity Networks under Interval Uncertainty
Dedicated to the memory of Professor Stefan Chanas - The extended abstract version of this paper has appeared in Proceedings of 11th International Conference on Principles and Practice of Constraint Programming (CP2005) ("Interval Analysis in Scheduling", Fortin et al. 2005)International audienceThis paper reconsiders the Project Evaluation and Review Technique (PERT) scheduling problem when information about task duration is incomplete. We model uncertainty on task durations by intervals. With this problem formulation, our goal is to assert possible and necessary criticality of the different tasks and to compute their possible earliest starting dates, latest starting dates, and floats. This paper combines various results and provides a complete solution to the problem. We present the complexity results of all considered subproblems and efficient algorithms to solve them
Equilibrium pricing and ordering policies in a two-echelon supply chain in the presence of strategic customers
A mathematical model for the multi-mode resource-constrained project scheduling problem with mode dependent time lags
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