1,420 research outputs found
Characterizing iron-nitrogen colimitation interactions in a chemostat model of freshwater environments
Nitrogen limitation in freshwater lakes is more common than previously understood, suggesting that nitrogen fixation rates by cyanobacteria are often insufficient to alleviate nitrogen deficiency. Nitrogen fixation rates may be restricted by iron bioavailability in pelagic freshwater environments. Continuous culturing techniques allow investigations of phytoplankton physiology at steady state, but simultaneous limitation by two or more nutrients has rarely been explored in continuous culture experiments. Here we report continuous chemostat cultures of Anabaena sp. with various low concentrations of nitrate and iron and demonstrate that iron concentrations below ca. 100 nM are limiting to growth. Addition of nitrate resulted in decreased biomass-specific nitrogen fixation rates and stimulated additional biomass accumulation, confirming that the cultures were iron-nitrogen colimited. Chemostat cultures of cyanobacteria provide an attractive new laboratory model to explore colimitation with great value for measuring the factors restricting nitrogen fixation rates and causing apparent nitrogen limitation in freshwater pelagic environments
WNK Kinase Signaling in Ion Homeostasis and Human Disease
This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.WNK kinases, along with their upstream regulators (CUL3/KLHL3) and downstream targets (the SPAK/OSR1 kinases and the cation-Cl- cotransporters [CCCs]), comprise a signaling cascade essential for ion homeostasis in the kidney and nervous system. Recent work has furthered our understanding of the WNKs in epithelial transport, cell volume homeostasis, and GABA signaling, and uncovered novel roles for this pathway in immune cell function and cell proliferation.This work was supported by a NIHNRCDP grant (K.T.K.), Simons Foundation grant #400947 (K.T.K.), March of Dimes Basil O’Connor Award (K.T.K.), and NIH grant DK93501 to E.D
How Sales Taxes Affect Customer and Firm Behavior: The Role of Search on the Internet
When a multichannel retailer opens its first retail store in a state, the firm is obligated to collect sales taxes on all Internet and catalog orders shipped to that state. This article assesses how opening a store affects Internet and catalog demand. The authors analyze purchase behavior among customers who live far from the retail store but must now pay sales taxes on catalog and Internet purchases. A comparable group of customers in a neighboring state serves as a control. The results show that Internet sales decrease significantly, but catalog sales are unaffected. Further investigation indicates that the difference in these outcomes is partly attributable to the ease with which customers can search for lower prices at competing retailers. The authors extend the analysis to a panel of multichannel firms and show that retailers that earn a large proportion of their revenue from direct channels avoid opening a first store in high-tax states. They conclude that current U.S. sales taxes laws have significant effects on both customer and firm behavior
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Foundation species loss affects vegetation structure more than ecosystem function in a northeastern USA forest
Loss of foundation tree species rapidly alters ecological processes in forested ecosystems. Tsuga canadensis, an hypothesized foundation species of eastern North American forests, is declining throughout much of its range due to infestation by the nonnative insect Adelges tsugae and by removal through pre-emptive salvage logging. In replicate 0.81-ha plots, T. canadensis was cut and removed, or killed in place by girdling to simulate adelgid damage. Control plots included undisturbed hemlock and mid-successional hardwood stands that represent expected forest composition in 50–100 years. Vegetation richness, understory vegetation cover, soil carbon flux, and nitrogen cycling were measured for two years prior to, and five years following, application of experimental treatments. Litterfall and coarse woody debris (CWD), including snags, stumps, and fallen logs and branches, have been measured since treatments were applied. Overstory basal area was reduced 60%–70% in girdled and logged plots. Mean cover and richness did not change in hardwood or hemlock control plots but increased rapidly in girdled and logged plots. Following logging, litterfall immediately decreased then slowly increased, whereas in girdled plots, there was a short pulse of hemlock litterfall as trees died. CWD volume remained relatively constant throughout but was 3–4× higher in logged plots. Logging and girdling resulted in small, short-term changes in ecosystem dynamics due to rapid regrowth of vegetation but in general, interannual variability exceeded differences among treatments. Soil carbon flux in girdled plots showed the strongest response: 35% lower than controls after three years and slowly increasing thereafter. Ammonium availability increased immediately after logging and two years after girdling, due to increased light and soil temperatures and nutrient pulses from leaf-fall and reduced uptake following tree death. The results from this study illuminate ecological processes underlying patterns observed consistently in region-wide studies of adelgid-infested hemlock stands. Mechanisms of T. canadensis loss determine rates, magnitudes, and trajectories of ecological changes in hemlock forests. Logging causes abrupt, large changes in vegetation structure whereas girdling (and by inference, A. tsugae) causes sustained, smaller changes. Ecosystem processes depend more on vegetation cover per se than on species composition. We conclude that the loss of this late-successional foundation species will have long-lasting impacts on forest structure but subtle impacts on ecosystem function.Organismic and Evolutionary Biolog
Dietary Linolenic Acid and Adjusted QT and JT Intervals in the National Heart, Lung, and Blood Institute Family Heart Study
OBJECTIVES The goal of this study was to examine whether higher consumption of total linolenic acid was associated with rate-adjusted QT and JT intervals (QTrr and JTrr, respectively). BACKGROUND Higher intake of fish omega-3 fatty acids and plant omega-3 such as alpha-linolenic acid is associated with lower risk of myocardial infarction. While long-chain omega-3 can inhibit ventricular arrhythmia, it is not known whether alpha-linolenic acid influences ventricular repolarization. METHODS We studied 3,642 subjects from the National Heart, Lung, and Blood Institute Family Heart study who were free of myocardial infarction, left ventricular hypertrophy, pacemaker, and with QRS <120 ms. We used the 95th percentile of the gender-specific distribution of QTrr and JTrr to define abnormally prolonged repolarization. Within each gender, we created age-and energy-adjusted tertiles of linolenic acid and used regression models for analyses. RESULTS Mean age was 50 years, and average intake of total linolenic acid was 0.74 g/day. There was an inverse association between consumption of linolenic acid and QTrr and JTrr (p for trend 0.001 and 0.0005, respectively). From the lowest (reference) to the highest gender-, age-, and energy-adjusted tertile of linolenic acid, multivariable adjusted odds ratios for prolonged QTrr were 1.0, 0.74 (95% confidence interval [CI] 0.57 to 0.96), and 0.59 (95% CI 0.44 to 0.77), respectively (p for trend 0.0003). Corresponding values for JTrr were 1.0, 0.73 (95% CI 0.52 to 1.03), and 0.59 (95% CI 0.40 to 0.87), respectively (p for trend 0.009). Exclusion of subjects taking drugs known to influence QT did not influence this association. CONCLUSIONS Higher intake of dietary linolenic acid might be associated with a reduced risk of abnormally prolonged repolarization in men and women
Ribbon worms (phylum Nemertea) from Bodega Bay, California: A largely undescribed diversity
The diversity of nemerteans along the Pacific coast of the United States is regarded as well characterized, but there remain many cryptic, undescribed, and “orphan” species (those known only in their larval form). Recent sampling of nemerteans in Oregon and Washington has begun to fill in these taxonomic gaps, but nemertean diversity in California has received relatively little attention over the past 60 years. During the summers of 2019 and 2020, nemertean specimens were collected from 20 locations in the Bodega Bay region of northern California, USA, including rocky intertidal shores, sandy beaches, mudflats, and other habitats. Based on morphological assessment and DNA sequence analysis (partial Cytochrome Oxidase I and 16S rRNA genes), our surveys identified 34 nemertean species. Only 13 of these (38%) can be confidently assigned to described species. Another 11 represent species that are new to science, including members of the genera Riserius, Nipponnemertes, Poseidonemertes, Zygonemertes, Nemertellina, Oerstedia, and three species of uncertain affiliation. The remaining ten species include undescribed or cryptic species of uncertain status that have been found previously along the Pacific Coast of North America. Our surveys also document extensions of known geographic ranges for multiple species, including the first records in California of Antarctonemertes phyllospadicola, Cephalothrix hermaphroditica, and Maculaura oregonensis. This is the first report of the genus Nemertellina in the northeast Pacific and Riserius in California. Overall, our findings highlight how much remains to be learned about the diversity and distribution of nemerteans in the northeast Pacific
The Pittsburgh Sloan Digital Sky Survey MgII Quasar Absorption-Line Survey Catalog
We present a catalog of intervening MgII quasar absorption-line systems in
the redshift interval 0.36 <= z <= 2.28. The catalog was built from Sloan
Digital Sky Survey Data Release Four (SDSS DR4) quasar spectra. Currently, the
catalog contains > 17,000 measured MgII doublets. We also present data on the
~44,600 quasar spectra which were searched to construct the catalog, including
redshift and magnitude information, continuum-normalized spectra, and
corresponding arrays of redshift-dependent minimum rest equivalent widths
detectable at our confidence threshold. The catalog is available on the web. A
careful second search of 500 random spectra indicated that, for every 100
spectra searched, approximately one significant MgII system was accidentally
rejected. Current plans to expand the catalog beyond DR4 quasars are discussed.
Many MgII absorbers are known to be associated with galaxies. Therefore, the
combination of large size and well understood statistics makes this catalog
ideal for precision studies of the low-ionization and neutral gas regions
associated with galaxies at low to moderate redshift. An analysis of the
statistics of MgII absorbers using this catalog will be presented in a
subsequent paper.Comment: AJ, in pres
A SPAK Isoform Switch Modulates Renal Salt Transport and Blood Pressure
SummaryThe renal thick ascending limb (TAL) and distal convoluted tubule (DCT) play central roles in salt homeostasis and blood pressure regulation. An emerging model suggests that bumetanide- and thiazide-sensitive NaCl transporters (NKCC2 and NCC) along these segments are phosphorylated and activated by WNK kinases, via SPAK and OSR1. Here, we show that a kidney-specific SPAK isoform, which lacks the kinase domain, inhibits phosphorylation of NCC and NKCC2 by full-length SPAK in vitro. Kidney-specific SPAK is highly expressed along the TAL, whereas full-length SPAK is more highly expressed along the DCT. As predicted from the differential expression, SPAK knockout in animals has divergent effects along TAL and DCT, with increased phosphorylated NKCC2 along TAL and decreased phosphorylated NCC along DCT. In mice, extracellular fluid volume depletion shifts SPAK isoform abundance to favor NaCl retention along both segments, indicating that a SPAK isoform switch modulates sodium avidity along the distal nephron
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