Underwater Ant House

The project seeks to design and test and submersible enclosure capable of sustaining oxygen breathing life. A mesh covered in a superhydrophobic coating can both restrain water up to a maximum pressure and retain a direct interface between air and water. Oxygen can transfer over this boundary if there is a sufficient difference in concentrations between the water and air. Multiple designs have been produced with the intent of testing and demonstrating these theories. 3D printing allowed for quick and customizable production of every component. Enclosures were made for testing the maximum allowable pressure and to fit an oxygen sensor. These mostly consisted of a rectangular frame with slotted to fit removable mesh slides. Oxygen concentration experiments were conducted with crickets as live subjects and were designed to analyze the transfer of oxygen through the mesh. The team ran tests to investigate additional factors including coating application and quality, the influence of the mesh substrate material on hydrophobic and coating properties, and mesh sizing influence. Testing indicates that there is sufficient oxygen transfer for small animals to survive. The enclosure can be submerged to approximately four centimeters before failure. This project demonstrates the feasibility of maintaining breathable air using a hydrophobic mesh enclosure and creates the opportunity for further investigation into possible uses of this technology.https://scholarscompass.vcu.edu/capstone/1212/thumbnail.jp

Materials Characterization of Additively Manufactured Components for Rocket Propulsion

To advance Additive Manufacturing (AM) technologies for production of rocket propulsion components the NASA Glenn Research Center (GRC) is applying state of the art characterization techniques to interrogate microstructure and mechanical properties of AM materials and components at various steps in their processing. The materials being investigated for upper stage rocket engines include titanium, copper, and nickel alloys. Additive manufacturing processes include laser powder bed, electron beam powder bed, and electron beam wire fed processes. Various post build thermal treatments, including Hot Isostatic Pressure (HIP), have been studied to understand their influence on microstructure, mechanical properties, and build density. Micro-computed tomography, electron microscopy, and mechanical testing in relevant temperature environments has been performed to develop relationships between build quality, microstructure, and mechanical performance at temperature. A summary of GRC's Additive Manufacturing roles and experimental findings will be presented

Rotation Distributions around the Kraft Break with TESS and Kepler: The Influences of Age, Metallicity, and Binarity

Stellar rotation is a complex function of mass, metallicity, and age and can be altered by binarity. To understand the importance of these parameters in main sequence stars, we have assembled a sample of observations that spans a range of these parameters using a combination of observations from The Transiting Exoplanet Survey Satellite (TESS) and the Kepler Space Telescope. We find that while we can measure rotation periods and identify other classes of stellar variability (e.g., pulsations) from TESS lightcurves, instrument systematics prevent the detection of rotation signals longer than the TESS orbital period of 13.7 days. Due to this detection limit, we also utilize rotation periods constrained using rotational velocities measured by the APOGEE spectroscopic survey and radii estimated using the Gaia mission for both TESS and Kepler stars. From these rotation periods, we 1) find we can track rotational evolution along discrete mass tracks as a function of stellar age, 2) find we are unable to recover trends between rotation and metallicity that were observed by previous studies, and 3) note that our sample reveals that wide binary companions do not affect rotation, while close binary companions cause stars to exhibit more rapid rotation than single stars.Comment: 19 pages, 13 figures, Accepted for publication in the Astrophysical Journa

Association between recent overdose and chronic pain among individuals in treatment for opioid use disorder

Chronic pain increases risk for opioid overdose among individuals with opioid use disorder. The purpose of this study is to evaluate the relationship between recent overdose and whether or not chronic pain is active. 3,577 individuals in treatment for opioid use disorder in 2017 or 2018 were surveyed regarding recent overdoses and chronic pain. Demographics from the 2017 Treatment Episode Data Set, which includes all U.S. facilities licensed or certified to provide substance use care, were used to evaluate the generalizability of the sample. χ2 tests and logistic regression models were used to compare associations between recent overdoses and chronic pain. Specifically, active chronic pain was associated with opioid overdose among people in treatment for opioid use disorder. Individuals with active chronic pain were more likely to have had a past month opioid overdose than those with no history chronic pain (adjusted OR = 1.55, 95% CI 1.16-2.08, p = 0.0003). In contrast, individuals with prior chronic pain, but no symptoms in the past 30 days, had a risk of past month opioid overdose similar to those with no history of chronic pain (adjusted OR = 0.88, 95% CI 0.66-1.17, p = 0.38). This suggests that the incorporation of treatment for chronic pain into treatment for opioid use disorder may reduce opioid overdoses

Restricting Bunk Space Allotments to 6 or 10 Inches has Minimal Impact on Growth Performance in Limit-Fed Receiving Cattle

Objective:The objective of our experiment was to determine if bunk allotments of 6, 10, 14, or 18 in per head in pens containing 18 to 28 head impacts growth performance of growing calves limit-fed a high-energy dietbased on corn and corn co-products. Study Description:A total of 332 crossbred heifers were blocked by source, stratified by individual arrival weight, and assigned to a pen. Pens were randomly assigned to one of four treatments: 6, 10, 14, and 18 in of bunk space per head. Pens contained 18 to 28 head per pen. Heifers were limit-fed once daily at 2.0% of body weight (BW) [dry matter (DM) basis] for a 56-day period. Results:Final BW and average daily gains (ADG) following the 56-day period did not differ (P≥ 0.20) among treatments. The standard deviation of ADG responded quadratically (P= 0.05) where variation in weight gain was greater in calves allotted 14 in of bunk compared with calves allotted 6, 10, or 18 in of bunk. The Bottom Line:Bunk allotments as low as 6 in per head did not reduce the growth performance of limit-fed growing cattle during a 56-day receiving period

Personalized ctDNA micro-panels can monitor and predict clinical outcomes for patients with triple-negative breast cancer

Circulating tumor DNA (ctDNA) in peripheral blood has been used to predict prognosis and therapeutic response for triple-negative breast cancer (TNBC) patients. However, previous approaches typically use large comprehensive panels of genes commonly mutated across all breast cancers. Given the reduction in sequencing costs and decreased turnaround times associated with panel generation, the objective of this study was to assess the use of custom micro-panels for tracking disease and predicting clinical outcomes for patients with TNBC. Paired tumor-normal samples from patients with TNBC were obtained at diagnosis (T0) and whole exome sequencing (WES) was performed to identify somatic variants associated with individual tumors. Custom micro-panels of 4-6 variants were created for each individual enrolled in the study. Peripheral blood was obtained at baseline, during Cycle 1 Day 3, at time of surgery, and in 3-6 month intervals after surgery to assess variant allele fraction (VAF) at different timepoints during disease course. The VAF was compared to clinical outcomes to evaluate the ability of custom micro-panels to predict pathological response, disease-free intervals, and patient relapse. A cohort of 50 individuals were evaluated for up to 48 months post-diagnosis of TNBC. In total, there were 33 patients who did not achieve pathological complete response (pCR) and seven patients developed clinical relapse. For all patients who developed clinical relapse and had peripheral blood obtained ≤ 6 months prior to relapse (n = 4), the custom ctDNA micro-panels identified molecular relapse at an average of 4.3 months prior to clinical relapse. The custom ctDNA panel results were moderately associated with pCR such that during disease monitoring, only 11% of patients with pCR had a molecular relapse, whereas 47% of patients without pCR had a molecular relapse (Chi-Square; p-value = 0.10). In this study, we show that a custom micro-panel of 4-6 markers can be effectively used to predict outcomes and monitor remission for patients with TNBC. These custom micro-panels show high sensitivity for detecting molecular relapse in advance of clinical relapse. The use of these panels could improve patient outcomes through early detection of relapse with preemptive intervention prior to symptom onset

Advanced Manufacturing Technology: Low Cost Upper Stage-Class Propulsion

No abstract availabl

Resistance to Wheat streak mosaic virus identified in synthetic wheat lines

Citation: Shoup Rupp, J. L., Simon, Z. G., Gillett-Walker, B., & Fellers, J. P. (2014). Resistance to Wheat streak mosaic virus identified in synthetic wheat lines. Retrieved from http://krex.ksu.eduWheat streak mosaic virus (WSMV) is an important pathogen in wheat that causes significant yield losses each year. WSMV is typically controlled using cultural practices such as the removal of volunteer wheat. Genetic resistance is limited. Until recently, no varieties have been available with major resistance genes to WSMV. Two resistance genes have been derived from Thinopyrum intermedium through chromosome engineering, while a third gene was transferred from bread wheat through classical breeding. New sources of resistance are needed and synthetic wheat lines provide a means of accessing genetic variability in wheat progenitors. A collection of wheat synthetic lines was screened for WSMV resistance. Four lines, 07-SYN-27, -106, -164, and -383 had significant levels of resistance. Resistance was effective at 18 °C and virus accumulation was similar to the resistant control, WGGRC50 containing Wsm1. At 25 °C, resistance was no longer effective and virus accumulation was similar to the susceptible control, Tomahawk

Neuropilin-1 antagonism in human carcinoma cells inhibits migration and enhances chemosensitivity

BACKGROUND: Neuropilin-1 (NRP1) is a non-tyrosine kinase receptor for vascular endothelial growth factor (VEGF) recently implicated in tumour functions.METHODS: In this study we used a specific antagonist of VEGF binding to the NRP1 b1 domain, EG3287, to investigate the functional roles of NRP1 in human carcinoma cell lines, non-small-cell lung A549, kidney ACHN, and prostate DU145 cells expressing NRP1, and the underlying mechanisms involved.RESULTS: EG3287 potently displaced the specific binding of VEGF to NRP1 in carcinoma cell lines and significantly inhibited the migration of A549 and ACHN cells. Neuropilin-1 downregulation by siRNA also decreased cell migration. EG3287 reduced the adhesion of A549 and ACHN cells to extracellular matrix (ECM), and enhanced the anti-adhesive effects of a beta 1-integrin function-blocking antibody. EG3287 increased the cytotoxic effects of the chemotherapeutic agents 5-FU, paclitaxel, or cisplatin on A549 and DU145 cells, through inhibition of integrin-dependent cell interaction with the ECM.CONCLUSIONS: These findings indicate that NRP1 is important for tumour cell migration and adhesion, and that NRP1 antagonism enhances chemosensitivity, at least in part, by interfering with integrin-dependent survival pathways. A major implication of this study is that therapeutic strategies targeting NRP1 in tumour cells may be particularly useful in combination with other drugs for combating tumour survival, growth, and metastatic spread independently of an antiangiogenic effect of blocking NRP1. British Journal of Cancer (2010) 102, 541-552. doi:10.1038/sj.bjc.6605539 www.bjcancer.com Published online 19 January 2010 (C) 2010 Cancer Research U